Review
Biotechnology & Applied Microbiology
Lucie A. Low, Christine Mummery, Brian R. Berridge, Christopher P. Austin, Danilo A. Tagle
Summary: OoCs, also known as microphysiological systems or 'tissue chips', have the potential to be informative at multiple stages of drug discovery and development, providing insights into normal human organ function and disease pathophysiology, as well as predicting the safety and efficacy of investigational drugs in humans. The field of OoCs has seen significant advances in recent years, but there are still challenges and opportunities that need to be addressed to fully realize their potential for translational research.
NATURE REVIEWS DRUG DISCOVERY
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Carly Strelez, Hannah Y. Jiang, Shannon M. Mumenthaler
Summary: Recreating "living organs" using groundbreaking organ-on-a-chip (OOC) technologies is revolutionizing drug discovery. Studies by Huh et al. and Ronaldson-Bouchard et al. highlight the advancements made over the past decade, from single organ functionality to interconnected organs, allowing for the examination of drug toxicities and disease pathogenesis in reconstituted tissues.
TRENDS IN BIOTECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Lingyu Sun, Feika Bian, Dongyu Xu, Yuan Luo, Yongan Wang, Yuanjin Zhao
Summary: This article provides an overview of the advances in biomaterials for the construction of organs-on-chips. It introduces the composition, structure, and fabrication techniques of biomaterials, and focuses on their functions and applications in organs-on-chips systems. The use of biomaterial-based organs-on-chips as alternative animal models and the challenges and prospects of further functionalization are also discussed.
MATERIALS HORIZONS
(2023)
Review
Biotechnology & Applied Microbiology
Yi Yang, Yin Chen, Liang Wang, Shihui Xu, Guoqing Fang, Xilin Guo, Zaozao Chen, Zhongze Gu
Summary: The combination of organ-on-a-chip technology and physiologically based pharmacokinetic modeling has the potential to improve drug development and personalized medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Lisette Van Os, Britta Engelhardt, Olivier T. Guenat
Summary: In order to develop new therapies for infections, understanding the human immune system during infection is crucial. Organ-on-chip models have proven to be valuable tools for tissue modeling, and incorporating an immune component is necessary to simulate complex biological responses. This tutorial review provides a practical guide on developing an organ-on-chip model to investigate immune cell migration during infection, including the design of the chip, establishment of chemotactic gradients, and incorporation of different cell types. The review also highlights the importance of the extracellular matrix in accurately modeling the interstitial space for immune cell migration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Zohreh Izadifar, Alexandra Sontheimer-Phelps, Bob A. Lubamba, Haiqing Bai, Cicely Fadel, Anna Stejskalova, Alican Ozkan, Queeny Dasgupta, Amir Bein, Abidemi Junaid, Aakanksha Gulati, Gautam Mahajan, Seongmin Kim, Nina T. LoGrande, Arash Naziripour, Donald E. Ingber
Summary: Studying mucus biology has been challenging due to the lack of physiologically relevant human in vitro models. However, recent progress has been made in the development of human organ-on-a-chip microfluidic culture models, focusing on the lung, colon, small intestine, cervix, and vagina. These models can reconstitute epithelial tissue barriers and physiologically relevant mucus layers, allowing for the study of mucus composition, mechanics, and structure in a biomimetic environment.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Cell Biology
Louis Jun Ye Ong, Xiwei Fan, Antonia Rujia Sun, Lin Mei, Yi-Chin Toh, Indira Prasadam
Summary: Osteoarthritis (OA) is a prevalent disease worldwide, affecting over 20% of the population and leading to decreased quality of life for patients. Due to the complexity of mimicking joint tissue's physiological environment, the study of OA pathophysiology primarily relies on animal models. However, recent advancements in microfluidic organ-on-chip (OoC) systems have shown promise in replicating and manipulating tissue physiological environments. By adapting these techniques, it is possible to create human-specific in vitro models that accurately capture the cellular processes involved in OA. This review provides a comprehensive summary of various microfluidic platforms used to mimic joint microenvironments, with implications for future platform design iterations.
Review
Chemistry, Multidisciplinary
Yaqing Wang, Peng Wang, Jianhua Qin
Summary: This review introduces two cutting-edge technologies, stem cell organoids and bioengineered organs-on-chips, which can construct biomimetic three-dimensional tissue or organ models that closely mimic human responses. It highlights the recent progress and potential utility of these models in virology research, particularly in addressing the COVID-19 pandemic and developing vaccines and therapies. It also discusses the future opportunities and challenges in the development of advanced human organ models for translational applications.
Review
Microbiology
Jens Puschhof, Cayetano Pleguezuelos-Manzano, Hans Clevers
Summary: In vitro co-culture models are important tools for studying interactions between microbiota and intestinal tissues. These models can replicate specific processes of microbe-epithelia interactions. By using different models of bacterial, viral, and parasitic infections, the advantages of each culture model can be highlighted.
CELL HOST & MICROBE
(2021)
Review
Multidisciplinary Sciences
Jintao Li, Jie Chen, Hua Bai, Haiwei Wang, Shiping Hao, Yang Ding, Bo Peng, Jing Zhang, Lin Li, Wei Huang
Summary: Microfluidic-based organs-on-chips (OoCs) are an advanced technology in biomedical and chemical research, offering unique properties for biomedical applications. However, the amount of data generated by OoC systems has surpassed manual analysis capabilities. Deep learning, a new area in machine learning, can automatically analyze big data and has successful applications in various fields. The integration of deep learning in OoCs shows great potential for drug development, disease modeling, and personalized medicine.
Article
Biochemical Research Methods
Noa Renous, Mark D. Kiri, Ronny A. Barnea, Rossana Rauti, Yael Leichtmann-Bardoogo, Ben M. Maoz
Summary: The S-TEER organ-on-a-chip platform introduces moving electrodes for localized electrical resistance measurements to monitor cellular growth and barrier function. This system shows particular potential for studying barrier function.
Review
Pharmacology & Pharmacy
Berivan Cecen, Christina Karavasili, Mubashir Nazir, Anant Bhusal, Elvan Dogan, Fatemeh Shahriyari, Sedef Tamburaci, Melda Buyukoz, Leyla Didem Kozaci, Amir K. Miri
Summary: Organ-on-a-chip technology is utilized for testing small-molecule drugs, with a focus on simulating interactions between drugs and the body. The development of multi-organ-on-a-chip (MOC) technology aims to replicate various organs involved in drug-body interactions. Current research in MOCs is particularly concentrated on studying the dynamic processes of drug absorption, distribution, metabolism, and excretion.
Review
Genetics & Heredity
Donald E. Ingber
Summary: This Review discusses the types of single and multiple human organ-on-a-chip (organ chip) microfluidic devices and their diverse applications for disease modeling, drug development, and personalized medicine. It also addresses the challenges that must be overcome for organ chips to reach their full potential and discusses recent advances in the field.
NATURE REVIEWS GENETICS
(2022)
Article
Biophysics
Anna Grazia Monteduro, Silvia Rizzato, Giusi Caragnano, Adriana Trapani, Gianluigi Giannelli, Giuseppe Maruccio
Summary: Current in-vitro 2D cultures and animal models have limitations in recapitulating human physiology, but microphysiological systems and organ-on-chip technologies are emerging as novel tools for high-throughput research and drug development. This review provides an introduction to major themes in this field and discusses the significance, limitations, and future prospects of these technologies.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Jonathan Sabate del Rio, Jooyoung Ro, Heejeong Yoon, Tae-Eun Park, Yoon-Kyoung Cho
Summary: Organs-on-chips (OoCs) are biomimetic in vitro systems that mimic the physicochemical microenvironments, physiologies, and key functional units of specific human organs using microfluidic cell cultures. These systems have the potential to replace animal models, enable personalized medicine, and require continuous monitoring of quality parameters. Integration of biosensing technologies into OoCs allows for monitoring of their physiologies, functions, and microenvironments. Future directions involve the application of artificial intelligence for process optimization, self-regulation, and data analysis in OoCs and cyber-physical systems.
BIOSENSORS & BIOELECTRONICS
(2023)
Meeting Abstract
Cell & Tissue Engineering
N. Moore, J. Chevillet, L. Healey, C. McBrine, D. Doty, J. Santos, B. Teece, V. Mott, V. Tandon, J. T. Borenstein, J. Balestrini, K. T. Kotz
Editorial Material
Pharmacology & Pharmacy
M. Peppi, A. Marie, C. Belline, J. T. Borenstein
EXPERT OPINION ON DRUG DELIVERY
(2018)
Article
Audiology & Speech-Language Pathology
Andrew M. Ayoob, Marcello Peppi, Vishal Tandon, Robert Langer, Jeffrey T. Borenstein
Article
Pharmacology & Pharmacy
Andrew M. Ayoob, Marcello Peppi, Vishal Tandon, Robert Langer, Jeffrey T. Borenstein
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Article
Engineering, Biomedical
Ashley L. Beckwith, Luis F. Velasquez-Garcia, Jeffrey T. Borenstein
ADVANCED HEALTHCARE MATERIALS
(2019)
Article
Multidisciplinary Sciences
Nathan Moore, John R. Chevillet, Laura J. Healey, Connor McBrine, Daniel Doty, Jose Santos, Bryan Teece, James Truslow, Vienna Mott, Peter Hsi, Vishal Tandon, Jeffrey T. Borenstein, Jenna Balestrini, Kenneth Kotz
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Daniel T. Doty, Julia Schueler, Vienna L. Mott, Cassie M. Bryan, Nathan F. Moore, John C. Ho, Jeffrey T. Borenstein
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Engineering, Biomedical
Alla A. Gimbel, James C. Hsiao, Ernest S. Kim, Diana J. Lewis, Thomas F. Risoleo, Joseph N. Urban, Jeffrey T. Borenstein
Summary: Advances in microfluidics have led to the development of a new generation of microfluidic respiratory assist devices, with potential for higher gas transfer efficiency due to shallower channels and thinner membranes compared to existing hollow fiber membrane oxygenators. However, scaling up microfluidic designs to support high blood flows for adult humans presents challenges in blood distribution strategies. Innovative design and fabrication strategies will be required to minimize pressure drops while maintaining high oxygen transfer efficiencies in next-generation microfluidic oxygenators.
Article
Chemistry, Analytical
Jose Santos, Else M. Vedula, Weixuan Lai, Brett C. Isenberg, Diana J. Lewis, Dan Lang, David Sutherland, Teryn R. Roberts, George T. Harea, Christian Wells, Bryan Teece, Paramesh Karandikar, Joseph Urban, Thomas Risoleo, Alla Gimbel, Derek Solt, Sahar Leazer, Kevin K. Chung, Sivaprasad Sukavaneshvar, Andriy Batchinsky, Jeffrey T. Borenstein
Summary: The recent development of microfluidic extracorporeal lung support technologies provides an opportunity to enhance gas transfer efficiency and improve hemocompatibility. A single-layer prototype with high flow rates has been developed, showing promising results for future applications in acute lung injury treatment.
Review
Engineering, Biomedical
Todd L. Astor, Jeffrey T. Borenstein
Summary: The increasing prevalence of chronic lung disease and respiratory virus pandemics highlights the need for safer and more effective artificial lung support. Mechanical ventilation is commonly used but carries risks. Microfluidics-based artificial organ support offers a potential solution by improving blood circulation and gas exchange, reducing complications.
Article
Biology
Ashley L. L. Gard, Rebeccah J. J. Luu, Ryan Maloney, Madeline H. H. Cooper, Brian P. P. Cain, Hesham Azizgolshani, Brett C. C. Isenberg, Jeffrey T. T. Borenstein, Jane Ong, Joseph L. L. Charest, Else M. M. Vedula
Summary: A microfluidic model of human gingival tissue allows for the study of disease progression and evaluation of therapeutic agents for gum disease. This model demonstrates physiological tissue structure and response to inflammatory triggers, providing a platform for identification of new therapeutic targets.
COMMUNICATIONS BIOLOGY
(2023)
Article
Engineering, Biomedical
A. A. Setty, T. Chiang, J. A. Santos, B. C. Isenberg, E. Vedula, R. Keating, D. W. Sutherland, J. T. Borenstein
Summary: Researchers have successfully demonstrated simultaneous blood gas oxygenation and fluid removal in a single microfluidic circuit, which could potentially revolutionize clinical therapy for respiratory support and fluid management.
Article
Immunology
Robert Jordan, Stephanie L. Ford-Scheimer, Rodolfo M. Alarcon, Anthony Atala, Jeffrey T. Borenstein, Kyle R. Brimacombe, Sara Cherry, Hans Clevers, Mindy Davis, Simon G. P. Funnell, Lee Gehrke, Linda G. Griffith, Abigail C. Grossman, Thomas Hartung, Donald E. Ingber, Nicole C. Kleinstreuer, Calvin J. Kuo, Emily M. Lee, Christine L. Mummery, Thames E. Pickett, Sasirekha Ramani, Edwin A. Rosado-Olivieri, Evi B. Struble, Zhengpeng Wan, Mark S. Williams, Matthew D. Hall, Marc Ferrer, Sarine Markossian
Summary: The National Center for Advancing Translational Sciences (NCATS) organized a virtual workshop on 3D tissue models for antiviral drug development on June 7-8, 2022. The workshop covered critical concepts to help scientists establish robust, reproducible, and scalable 3D tissue models for studying viruses with pandemic potential. Experts from academia, industry, and government provided an overview of the utility and limitations of 3D tissue models, shared case studies and practical advice, and discussed the challenges and perspectives of using 3D tissues in antiviral drug discovery.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Cell Biology
Christine R. Fisher, Felix Mba Medie, Rebeccah J. Luu, Robert B. Gaibler, Thomas J. Mulhern, Caitlin R. Miller, Chelsea J. Zhang, Logan D. Rubio, Elizabeth E. Marr, Vidhya Vijayakumar, Elizabeth P. Gabriel, Landys Lopez Quezada, Chun-Hui Zhang, Karen S. Anderson, William L. Jorgensen, Jehan W. Alladina, Benjamin D. Medoff, Jeffrey T. Borenstein, Ashley L. Gard
Summary: This study reports SARS-CoV-2 infection and robust viral replication in the high-throughput, human primary cell-based organ-on-chip platform called PREDICT96-ALI. The unique infection kinetic profiles of lung tissue from three human donors were evaluated using immunofluorescence, RT-qPCR, and plaque assays. The antiviral efficacy of Remdesivir and MPro61 was also investigated in this system.
Review
Biochemical Research Methods
Jeffrey T. Borenstein, Gerard Cummins, Abhishek Dutta, Eyad Hamad, Michael Pycraft Hughes, Xingyu Jiang, Hyowon (Hugh) Lee, Kin Fong Lei, Xiaowu (Shirley) Tang, Yuanjin Zheng, Jie Chen
Summary: The development of micro- and nanotechnology in biomedical applications has been widely applied, especially in solving complex problems in medicine and biology. This paper reviews the latest advancements and challenges in bionanotechnology and bioMEMS, including applications in microfluidics, drug delivery, infectious disease management, and microdevice fabrication.
