Article
Immunology
Eugenia A. Panova, Denis A. Kleymenov, Dmitry V. Shcheblyakov, Evgeniia N. Bykonia, Elena P. Mazunina, Alina S. Dzharullaeva, Anastasia N. Zolotar, Artem A. Derkaev, Ilias B. Esmagambetov, Ivan I. Sorokin, Evgeny V. Usachev, Anatoly N. Noskov, Igor A. Ivanov, Timofei S. Zatsepin, Sergey E. Dmitriev, Vladimir A. Gushchin, Boris S. Naroditsky, Denis Y. Logunov, Alexander L. Gintsburg
Summary: Single-domain antibodies (sdAbs, VHHs, or nanobodies) are small-sized antibodies that show promise in treating infectious and somatic diseases. Their ability to bind hard-to-reach antigenic epitopes makes them valuable for therapy. Fusion of VHH with the immunoglobulin Fc fragment enhances their neutralizing activity and half-life.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Physical
Noe Escareno, Natalia Hassan, Marcelo J. Kogan, Josue Juarez, Antonio Topete, Adrian Daneri-Navarro
Summary: Nanoparticle-based drug delivery systems combined with high-affinity targeting ligands provide a sophisticated landscape in cancer theranostics. Antibodies, due to their high diversity and specificity, are extensively used to provide bioactivity to nanoparticulate systems. However, controlled assembly of nanoparticles with targeting ligands remains a challenge.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Food Science & Technology
Sara Samadzadeh, Raphaela Brauns, Harald Hefter
Summary: This study demonstrates the diversity of responses to Botulinum toxin therapy in cervical dystonia patients, including hypersensitivity, poor response, lack of response, development of neutralizing antibodies, etc. These findings suggest that patient responses to therapy may be unexpected and require cautious monitoring and adjustment of treatment strategies.
Article
Food Science & Technology
Doris M. Snow, Ronald R. Cobb, Juan Martinez, Isaac Finger-Baker, Laura Collins, Sara Terpening, Emily S. Syar, Nancy Niemuth, Dean Kobs, Roy Barnewall, Shauna Farr-Jones, James D. Marks, Milan T. Tomic
Summary: Botulinum neurotoxins are highly potent toxins that require long-term intensive care to prevent death. Current research is focused on developing monoclonal antibodies that can neutralize the toxins, with promising results shown by a six-antibody lyophilized formulation G03-52-01 that can protect against both type A and B toxins.
Article
Food Science & Technology
Consuelo Garcia-Rodriguez, Shude Yan, Isin N. Geren, Kristeene A. Knopp, Jianbo Dong, Zhengda Sun, Jianlong Lou, Fraser Conrad, Wei-Hua Wen, Shauna Farr-Jones, Theresa J. Smith, Jennifer L. Brown, Janet C. Skerry, Leonard A. Smith, James D. Marks
Summary: This study developed a four-antibody combination targeting different botulinum neurotoxin serotypes, with the ability to high-affinity bind and neutralize multiple serotypes and their mosaic toxins. These antibodies have potential applications in therapeutics and diagnostics.
Article
Biophysics
Veronica Caratelli, Silvia Fillo, Nino D'Amore, Ornella Rossetto, Marco Pirazzini, Maria Moccia, Concetta Avitabile, Danila Moscone, Florigio Lista, Fabiana Arduini
Summary: A paper-based antibody-free sensor has been developed for rapid and reliable detection of BoNT/A and BoNT/C, with a high linear range and low detection limit. Testing with spiked orange juice samples showed that the sensor has high accuracy and reliability in practical applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Alexei Subekin, Rugiya Alieva, Vladimir Kukushkin, Ilya Oleynikov, Elena Zavyalova
Summary: Surface-enhanced Raman scattering (SERS) is a powerful technique for the detection of analytes. In this paper, a SERS-based sensor was proposed for the rapid and sensitive detection of botulinum toxin type A using a functionalized silver nanoisland substrate. The proposed sensor architecture allows for one-stage detection without additional staining, with a limit of detection of 2.4 ng/mL.
Article
Multidisciplinary Sciences
Jean Mukherjee, Celinia A. Ondeck, Jacqueline M. Tremblay, Jacob Archer, Michelle Debatis, Alexa Foss, Junya Awata, Jesse H. Erasmus, Patrick M. McNutt, Charles B. Shoemaker
Summary: Single domain antibodies (sdAbs) have advantages in targeting multiple antigens and improving target affinity. Testing different sdAb heterohexamers has shown neutralizing effects on three Botulinum neurotoxin serotypes. Long sdAb heteromultimers as proteins or repRNA offer versatility in antibody-based therapeutic development.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Joseph D. Cleveland, Amir Taslimi, Qi Liu, Anna M. Van Keuren, Mair E. A. Churchill, Chandra L. Tucker
Summary: Proteases with reprogrammed specificity for non-native substrates are highly desired in synthetic biology and biomedicine. In this work, researchers engineered an orthogonal botulinum neurotoxin serotype B (BoNT/B) protease that recognizes an orthogonal substrate to expand the versatility of protease systems. They designed and validated an orthogonal BoNT/B protease system in mammalian cells and demonstrated its application in regulating complex transcriptional circuitry. The platform was also successfully applied for in vivo protease evolution in yeast and used to identify novel orthogonal protease/protease substrate combinations through high-throughput screening. In addition, the researchers developed new cleavage reporters for reporting botulinum toxin protease activity in mammalian cells.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Surgery
Kyu-Ho Yi, Hyung-Jin Lee, Hye-Won Hur, Kyle K. Seo, Hee-Jin Kim
Summary: This study reviewed the anatomical considerations when injecting botulinum neurotoxin and provided optimal injection sites and technique for the temporalis and masseter muscles. It also proposed external anatomical landmarks for injection in frequently injected muscles of the face.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2022)
Article
Biochemistry & Molecular Biology
Sebastien Brier, Christine Rasetti-Escargueil, Anne Wijkhuisen, Stephanie Simon, Maud Marechal, Emmanuel Lemichez, Michel R. Popoff
Summary: The study identified the unique neutralization mechanism of TA12, demonstrating that its conformational epitope recognizes the specific region at the interface of the BoNT/A1 receptor-binding domain and overlaps with the binding site for the neuronal cell surface receptors SV2C and GT1b. TA12 effectively blocks the entry of BoNT/A1 into neurons by interfering with the binding of SV2C and, to a lesser extent, GT1b, highlighting the potential of using single mAbs for the treatment of botulism type A.
Article
Food Science & Technology
Mariam Hull, Mered Parnes, Joseph Jankovic
Summary: Self-injurious behaviors are repetitive and persistent actions that cause physical harm to oneself. They are often seen in individuals with neurodevelopmental and neuropsychiatric conditions, particularly those with intellectual disability. These behaviors can be severe and life-threatening, making them difficult to treat. However, the use of botulinum neurotoxin injections has shown promise in preventing or reducing self-injury in children.
Article
Food Science & Technology
Michiel M. Harmsen, Jan C. Cornelissen, Fimme J. van der Wal, Jan H. W. Bergervoet, Miriam Koene
Summary: This study produced 30 novel and 2 earlier published llama single-domain antibodies (VHHs) against veterinary-relevant Botulinum neurotoxin serotypes C and D using yeast. These VHHs exhibited high affinity and were suitable for the development of sensitive Endopep-MS assays, as well as detection of native Botulinum neurotoxins from complex matrices. Additionally, these VHHs could be used for the development of novel therapeutics for animals or humans.
Review
Food Science & Technology
Parisa Gazerani
Summary: There is still no approved product for the anti-itch indication of botulinum neurotoxins (BoNTs) after two decades of research. Most studies focus on the off-label use of BoNTs for various itchy conditions, with controversial results from few randomized clinical trials. To obtain approval for the anti-pruritic indication of BoNTs, higher levels of evidence are required through well-designed studies.
Review
Food Science & Technology
Flavia Antonucci, Yuri Bozzi
Summary: Botulinum neurotoxins (BoNTs) are zinc endopeptidases that can inhibit the release of neurotransmitters, potentially providing therapeutic effects in certain neurological disorders. However, their efficacy is limited in humans.
Article
Endocrinology & Metabolism
Ying Cui, Hui Huang, Wenkai Ren, Ying Xu, Xiaoming Zha, Ming Zeng, Zhanhui Gao, Shaowen Tang, Guang Yang, Yaoyu Huang, Fangyan Xu, Hanyang Qian, Wenbin Zhou, Chun Ouyang, Lina Zhang, Xueyan Gao, Jing Zhang, Jing Wang, Jing Guo, Changying Xing, Yongyue Wei, Ningning Wang
Summary: Nondipping heart rate pattern in CKD5 patients is a prognostic marker for all-cause mortality. PTX may mediate a better outcome by reversing the nondipping heart rate ratio in SHPT patients.
ENDOCRINE PRACTICE
(2022)
Article
Cell Biology
Lianju Qin, Jing Zhang, Yujie Xiao, Kang Liu, Yugui Cui, Fangyan Xu, Wenkai Ren, Yanggang Yuan, Chunyan Jiang, Song Ning, Xiaoxue Ye, Ming Zeng, Hanyang Qian, Anning Bian, Fan Li, Guang Yang, Shaowen Tang, Zhihong Zhang, Juncheng Dai, Jing Guo, Qiang Wang, Bin Sun, Yifei Ge, Chun Ouyang, Xueqiang Xu, Jing Wang, Yaoyu Huang, Hongqing Cui, Jing Zhou, Meilian Wang, Zhonglan Su, Yan Lu, Di Wu, Jingping Shi, Wei Liu, Li Dong, Yinbing Pan, Baiqiao Zhao, Ying Cui, Xueyan Gao, Zhanhui Gao, Xiang Ma, Aiqin Chen, Jie Wang, Meng Cao, Qian Cui, Li Chen, Feng Chen, Youjia Yu, Qiang Ji, Zhiwei Zhang, Mufeng Gu, Xiaojun Zhuang, Xiaolin Lv, Hui Wang, Yanyan Pan, Ling Wang, Xianrong Xu, Jing Zhao, Xiuqin Wang, Cuiping Liu, Ningxia Liang, Changying Xing, Jiayin Liu, Ningning Wang
Summary: This study reported a case of severe uremic calciphylaxis patient who showed significant improvement after receiving treatment with human amnion-derived mesenchymal stem cells (hAMSCs). The long-term intravenous combined with local treatment of hAMSCs may be a potential regenerative treatment for uremic calciphylaxis.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei Guo, Mingyong Zeng, Suqin Zhu, Shiyang Li, Yilin Qian, Haohao Wu
Summary: Phycocyanin and phycocyanobilin have been found to have anti-colitis effects and are more effective than the traditional drug mesalazine. They can alleviate colitis by protecting the intestinal epithelial barrier and inhibiting inflammation.
Article
Biochemistry & Molecular Biology
Yuan Li, Jiayin Huang, Zhiheng Hu, Mingyong Zeng, Zunying Liu, Yaqin Hu
Summary: An inclusion system of embedding ferulic acid into beta-cyclodextrin was prepared and evaluated for its physicochemical properties and release kinetics. The results confirmed the successful embedding of ferulic acid and identified different release models under various host-guest stoichiometries. The FACD inclusion complex shows promise as a bioactive material for food preservation.
Article
Immunology
Feng-Cai Zhu, Hao Zeng, Jing-Xin Li, Bin Wang, Fan-Yue Meng, Feng Yang, Jiang Gu, Hao-Yu Liang, Yue-Mei Hu, Pei Liu, Liu-Sheng Peng, Xiao-Kui Hu, Yuan Zhuang, Min Fan, Hai-Bo Li, Zhong-Ming Tan, Ping Luo, Peng Zhang, Kai Chu, Jin-Yong Zhang, Ming Zeng, Quan-Ming Zou
Summary: This study evaluated the safety and immunogenicity of a recombinant five-antigen Staphylococcus aureus vaccine in healthy adults. The vaccine was found to be safe and well-tolerated, and it elicited strong humoral and cellular immune responses. Further research and development of this vaccine are warranted.
Article
Chemistry, Physical
Yueliuting Fu, Cui-Hong Chen, Mao-Gui Huang, Jun-Yang Tao, Xu Peng, Hai-Bing Xu, Yue-Jin Liu, Ming-Hua Zeng
Summary: In this study, we report a method for the site-selective functionalization of naphthalene at the remote C5 position using tertiary phosphine-induced ruthenium-catalyzed delta-bond activation. This strategy allows for efficient installation of different functional groups at the C5 position with excellent regioselectivity. Preliminary mechanistic studies reveal the essential roles of the tertiary phosphine group in the reaction.
Article
Reproductive Biology
Jie Li, Tianliu Peng, Le Wang, Panpan Long, Ruping Quan, Hangjing Tan, Minghua Zeng, Xue Wu, Junting Yang, Hongmei Xiao, Xiaobo Shi
Summary: This study identified a novel gene possibly associated with POI through whole exome sequencing, and demonstrated its functional impact on the development of POI.
JOURNAL OF OVARIAN RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Yunan Qin, Jingpeng Xiong, Quan Li, Yuexing Zhang, Ming-Hua Zeng
Summary: In this study, a M2L4-type metal-organic nanocage with four endohedral Feringa's motor motifs was constructed, and its adaptive encapsulation towards a C-60 guest molecule was demonstrated. The structure of the cage was characterized by a combination of ESI-MS, 2D DOSY, C-13 NMR, and SAXS techniques. The motor within the cage showed similar photophysical properties to the uncoordinated one, indicating its functionality was not compromised. The nanocage exhibited good guest encapsulation ability towards C-60 and revealed a guest induced-fit behavior based on extensive SAXS analysis and molecular dynamics simulation.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Chemistry, Applied
Yuyang Ma, Jinjin Xu, Suisui Jiang, Mingyong Zeng
Summary: In this study, oyster protein hydrolysate was prepared by enzymatic hydrolysis and loaded into nanoliposomes. Chitosan coating significantly improved the physical stability and antioxidant activity retention of nanoliposomes, playing an important role in powder reconstitution process.
Article
Chemistry, Physical
Baojun Long, Peiyu Cao, Yuanmeng Zhao, Qianqian Fu, Yan Mo, Yueming Zhai, Juejing Liu, Xingyi Lyu, Tao Li, Xiaofeng Guo, Changsheng Shan, Minghua Zeng
Summary: This study reports a novel Pt single-atom catalyst for highly efficient electrochemical catalysis and sensing of glucose. The loading of Pt single atoms increases with Co doping, providing more anchoring sites for Pt SAs. The resulting Pt-1/Ni(6)Co(1)LDHs/NG exhibits low oxidative potential, high sensitivity, excellent selectivity, and great stability. The boosted performance of Pt-1/Ni(6)Co(1)LDHs/NG originates from its stronger binding energy with glucose and the synergistic effect of Pt SAs, Co doping, and NG.
Article
Chemistry, Applied
Zihao Yin, Mengwei Wang, Mingyong Zeng
Summary: In this study, aqueous protein extraction from H. pluvialis residues (APHRs) was successfully extracted, which had high content of protein and carbohydrate. APHRs showed excellent emulsifying stability and oil holding capacity, and microscopic observation revealed the presence of fibrous polysaccharides that formed stable structural cross-linking around protein particles. Compared to other residues, APHRs formed emulsions with the smallest particle size and best storage stability.
FOOD HYDROCOLLOIDS
(2023)
Article
Plant Sciences
Ming Zeng, Nicole M. van Dam, Bettina Hause
Summary: Ethylene (ET) controls various aspects of plant growth and development under both abiotic and biotic stresses. MtEIN2, a critical element in the ET signaling pathway, plays an important role in biotic interactions. However, its role in responding to abiotic stress, such as nutrient deficiency, is less understood.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Astronomy & Astrophysics
X. P. Geng, L. T. Yang, Q. Yue, K. J. Kang, Y. J. Li, H. P. An, C. Greeshma, J. P. Chang, Y. H. Chen, J. P. Chang, W. H. Dai, Z. Deng, C. H. Fang, H. Gong, Q. J. Guo, X. Y. Guo, L. He, S. M. He, J. W. Hu, H. X. Huang, T. C. Huang, H. T. Jia, X. Jiang, S. Karmakar, H. B. Li, J. M. Li, J. Li, Q. Y. Li, R. M. J. Li, X. Q. Li, Y. L. Li, Y. F. Liang, B. Liao, F. K. Lin, S. T. Lin, J. X. Liu, S. K. Liu, Y. D. Liu, Y. Liu, Y. Y. Liu, Z. Z. Liu, H. Ma, Y. C. Mao, Q. Y. Nie, J. H. Ning, H. Pan, N. C. Qi, J. Ren, X. C. Ruan, Z. She, M. K. Singh, T. X. Sun, C. J. Tang, W. Y. Tang, Y. Tian, G. F. Wang, L. Wang, Q. Wang, Y. F. Wang, Y. X. Wang, H. T. Jia, S. Y. Wu, Y. C. Wu, H. Y. Xing, R. Xu, Y. Xu, T. Xue, Y. L. Yan, N. Yi, C. X. Yu, H. J. Yu, J. F. Yue, M. Zeng, Z. Zeng, B. T. Zhang, F. S. Zhang, L. Zhang, Z. H. Zhang, Z. Y. Zhang, K. K. Zhao, M. G. Zhao, J. F. Zhou, Z. Y. Zhou, J. J. Zhu, Y. C. Wu
Summary: We investigate exotic neutrino interactions using data from the CDEX-10 experiment at the China Jinping Underground Laboratory. New constraints on the mass and couplings of new gauge bosons are presented. Two nonstandard neutrino interactions involving a UO1 thorn B-L gauge boson and a dark photon are considered.
Article
Chemistry, Physical
Lu Wu, Jin Lin, Linyuan Ren, Qianni Li, Xin Chi, Ling Luo, Yuexing Zhang, Ming-Hua Zeng
Summary: Different surface planes of various types of titanium dioxide crystals have diverse effects on the splitting of H2O and H-2, as well as the electronic structures of hydroxylated titanium dioxide. Particularly, the electronic structures of R-101, R-001, A-110, A-100, and A-001 surfaces are significantly influenced by hydroxylation.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Wen-Tao Ma, Mao-Gui Huang, Yueliuting Fu, Zhong-Hui Wang, Jun-Yang Tao, Jia-Wei Li, Yue-Jin Liu, Ming-Hua Zeng
Summary: We present a phosphine-directed ruthenium-catalyzed C8-selective alkylation of naphthalenes with alkenes. This protocol provides a straightforward approach to access a vast library of electron-rich C8-alkyl substituent 1-naphthphosphines, which demonstrate improved performance compared to common commercial phosphines and their precursors in Pd-catalyzed Suzuki-Miyaura coupling of aryl bromides with alkylboronic acid.
CHEMICAL COMMUNICATIONS
(2022)