Review
Cell Biology
Niccolo Candelise, Francesca Santilli, Jessica Fabrizi, Daniela Caissutti, Zaira Spinello, Camilla Moliterni, Loreto Lancia, Simona Delle Monache, Vincenzo Mattei, Roberta Misasi
Summary: Despite the lack of available therapies for neurodegenerative disorders, cell therapy using stem cells from various sources, such as dental pulp stem cells, has shown promise in preclinical models. These stem cells can be reprogrammed for neural differentiation and have potential applications in the treatment of nervous system disorders. This review summarizes the advancements and differences in the maintenance, differentiation, and potential clinical application of such stem cells.
Review
Biotechnology & Applied Microbiology
Forough Mahdavi-Jouibari, Benyamin Parseh, Ezatolah Kazeminejad, Ayyoob Khosravi
Summary: Cartilage lesions are common and challenging to regenerate. Stem cell-based therapies, especially using Mesenchymal stem cells (MSCs) and Stem cells from human exfoliated deciduous teeth (SHED), offer promising solutions for cartilage regeneration. MSCs can differentiate into mature chondrocytes and engraft into the host, while SHED-derived secretome contains biomolecules that promote tissue regeneration, including cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Dua Abuarqoub, Rand Zaza, Nazneen Aslam, Hanan Jafar, Suzan Zalloum, Renata Atoom, Abdalla Awidi
Summary: This study investigates the effect of Biodentine(TM) on odonto/osteogenic differentiation potential of hDPSCs in vitro. The results show that treatment with BD significantly increases the expression of odonto/osteogenic markers, upregulates genes related to the odonto/osteogenesis pathway, and enhances calcium deposits, suggesting BD as a promising candidate for regenerative medicine in dental tissue regeneration.
APPLIED SCIENCES-BASEL
(2021)
Article
Dentistry, Oral Surgery & Medicine
Chung-Min Kang, Min Kyung Shin, Mijeong Jeon, Yong-Hyuk Lee, Je Seon Song, Jae-Ho Lee
Summary: Comparative research on the cytokine profiles secreted by stem cells from human exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSCs) revealed significant differences in the expression levels of multiple cytokines. SHED showed stronger expression of cytokines related to immunomodulation, odontogenesis, and osteogenesis, while DPSCs exhibited higher expression of angiogenesis-related cytokines. This indicates that SHED and DPSCs have distinct cytokine profiles and regenerative potential characteristics.
JOURNAL OF DENTAL SCIENCES
(2022)
Article
Cell Biology
Ning Wang, Xiao Han, Haoqing Yang, Dengsheng Xia, Zhipeng Fan
Summary: The study demonstrates that miR-6807-5p promotes odontogenic differentiation of dental pulp stem cells by silencing METTL7A, while METTL7A promotes odontogenic differentiation of dental pulp stem cells by binding with SNRNP200.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Tomoyuki Ueda, Taisei Ito, Masatoshi Inden, Hisaka Kurita, Akihito Yamamoto, Isao Hozumi
Summary: This study investigated the potential of stem cells from human exfoliated deciduous teeth (SHED) for the treatment of ALS. The study found that SHED-conditioned medium (CM) significantly reduced mutant SOD1-induced intracellular aggregates and neurotoxicity. The neuroprotective effects of SHED-CM were partly attributed to heat shock protein and the activation of insulin-like growth factor-1 receptor. In addition, SHED-CM also showed a protective effect on induced pluripotent stem cell-derived motor neurons and was effective against both familial and sporadic ALS. These findings suggest that SHED-CM could be a promising treatment for slowing the progression of ALS.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Environmental Sciences
Shiwen Deng, Caifeng Li, Junqi Chen, Zhao Cui, Tong Lei, Hongjun Yang, Peng Chen
Summary: This study evaluated the effects of TCS exposure on human stem cells and its molecular mechanisms. It was found that TCS inhibited cell proliferation and differentiation, induced senescence, mitochondrial dysfunction, ER stress, and oxidative stress, and significantly induced apoptosis and autophagy flux inhibition in high concentrations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Polymer Science
Vinna K. Sugiaman, Rudy Djuanda, Natallia Pranata, Silvia Naliani, Wayan L. Demolsky, Jeffrey
Summary: Maintaining dental pulp vitality and preventing tooth loss are challenges in endodontic treatment. Tissue engineering, especially using stem cells, has emerged as a potential alternative therapy for regenerating dental tissues.
Article
Biochemistry & Molecular Biology
Huong Thu Vu, Mi-Ran Han, Jun-Haeng Lee, Jong-Soo Kim, Ji-Sun Shin, Ji-Young Yoon, Jeong-Hui Park, Khandmaa Dashnyam, Jonathan Campbell Knowles, Hae-Hyoung Lee, Jong-Bin Kim, Jung-Hwan Lee
Summary: This study investigates the potential of conditioned media derived from stem cells of human exfoliated deciduous teeth (SHED CM) on the regeneration of dental pulp stem cells (DPSCs). The results demonstrate that SHED CM promotes the proliferation, survival, migration, and odontoblastic differentiation of DPSCs. The analysis of SHED CM reveals the presence of various cytokines and growth factors that positively regulate cell function. These findings suggest that SHED CM could be beneficial for pulp regeneration.
Review
Cell Biology
Wei Xiong, Ye Liu, Heng Zhou, Shuili Jing, Yan He, Qingsong Ye
Summary: Alzheimer's disease is a destructive neurodegenerative disease with a lack of effective drug candidates. Dental pulp stem cells have the potential for neuronal differentiation and neuroprotection, making them a promising approach for the treatment and protection of damaged neurons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Xiaojing Yuan, Zuoying Yuan, Yuanyuan Wang, Zhuo Wan, Xiaotong Wang, Shi Yu, Jianmin Han, Jianyong Huang, Chunyang Xiong, Lihong Ge, Qing Cai, Yuming Zhao
Summary: This study presents a novel injectable system that incorporates stem cells from human exfoliated deciduous teeth (SHEDs) and simvastatin (SIM) into gelatin methacrylate (GelMA) cryogel microspheres (SMS). The system promotes SHEDs adhesion, proliferation, odontogenic differentiation, and angiogenic potential. Subcutaneous implantation of SHEDs/SMS induces vessel-rich pulp-like tissue regeneration, highlighting the potential for clinical application in endodontic regenerative dentistry.
MATERIALS TODAY BIO
(2022)
Review
Cell & Tissue Engineering
Minu Anoop, Indrani Datta
Summary: This review discusses the potential therapeutic role of stem cells derived from human dental pulp tissue (SHED) in neurodegenerative diseases. SHED has several advantages but also some limitations, which require further research and improvement.
CURRENT STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Nathalia C. Oliveira, Fabiele B. Russo, Patricia Beltrao-Braga
Summary: This study aimed to establish a method for differentiating sensory neurons from induced pluripotent stem cells (iPSCs) derived from human exfoliated deciduous teeth (SHED), which have the same embryonic origin as the peripheral nervous system (PNS). The protocol successfully differentiated neural crest and PNS cells from SHED-derived iPSCs, providing a platform for future studies on PNS pathologies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Pooja Yadav, Ravina Vats, Afsareen Bano, Ritu Namdev, Rashmi Bhardwaj
Summary: This study systematically evaluated the therapeutic potential of Stem cells from human exfoliated deciduous teeth (SHED) in various diseases and found significant therapeutic effects in periodontitis, spinal cord injury, Parkinson's disease, Alzheimer's disease, etc. It also suggested the implementation of SHED in clinical trials after conducting more preclinical studies.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Soichiro Sonoda, Koichiro Yoshimaru, Haruyoshi Yamaza, Ratih Yuniartha, Toshiharu Matsuura, Erika Yamauchi-Tomoda, Sara Murata, Kento Nishida, Yoshinao Oda, Shouichi Ohga, Tasturo Tajiri, Tomoaki Taguchi, Takayoshi Yamaza
Summary: The study found that BA-specific post-natal stem cells are not suitable as a source for treatment, possibly due to epigenetic modifications in the prenatal and perinatal BA environments. Additionally, these studies may help in understanding the molecular mechanisms underlying BA development.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Materials Science, Characterization & Testing
Imran Alam Moheet, Norhayati Luddin, Ismail Ab Rahman, Sam'an Malik Masudi, Thirumulu Ponnuraj Kannan, Nik Rozainah Nik Abd Ghani
POLYMERS & POLYMER COMPOSITES
(2020)
Article
Biochemistry & Molecular Biology
Muhammad Fuad Hilmi Yusof, Siti Nurnasihah Md Hashim, Wafa' Zahari, Hamshawagini Chandra, Khairul Bariah Ahmad Amin Noordin, Thirumulu Ponnuraj Kannan, Suzina Sheikh Abdul Hamid, Khairani Idah Mokhtar, Ahmad Azlina
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Multidisciplinary Sciences
Nasar Um Min Allah, Zurairah Berahim, Azlina Ahmad, Kannan Thirumulu Ponnuraj
Article
Polymer Science
Hii Siew Ching, Kannan Thirumulu Ponnuraj, Norhayati Luddin, Ismail Ab Rahman, Nik Rozainah Nik Abdul Ghani
Article
Polymer Science
Sami D. Aldhuwayhi, Arbaz Sajjad, Wan Zaripah W. Bakar, Dasmawati Mohamad, Thirumulu P. Kannan, Imran A. Moheet
Summary: This study investigated the effects of adding a nano zirconia-silica-hydroxyapatite composite to a conventional glass ionomer cement, showing significant improvements in fracture toughness and color stability. The results suggest that the nanoZrO(2)-SiO2-HA composite has potential as a restorative dental material.
INTERNATIONAL JOURNAL OF POLYMER SCIENCE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Suhailiza Saharudin, Sarliza Yasmin Sanusi, Kannan Thirumulu Ponnuraj
Summary: This study aimed to conduct sequencing analysis of RUNX2 in non-syndromic patients with supernumerary teeth. Results showed no mutations in exons 5 and 6 of RUNX2 were identified through DNA sequencing analysis.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Polymer Science
Imran Alam Moheet, Norhayati Luddin, Ismail Ab Rahman, Sam'an Malik Masudi, Thirumulu Ponnuraj Kannan, Nik Rozainah Nik Abd Ghani
Summary: The study aimed to investigate the transfer of specific elements between nano-HA-SiO2-GIC and human enamel and dentine, suggesting potential increased remineralization with nano-HA-SiO2-GIC compared to conventional GIC (Fuji IX).
Article
Polymer Science
Ali Azhar Dawasaz, Rafi Ahmad Togoo, Zuliani Mahmood, Ahmad Azlina, Kannan Thirumulu Ponnuraj
Summary: Due to limitations of fluoride therapy in dental caries prevention, newer preventive agents like P11-4 peptide have been developed. P11-4 peptide can facilitate biomimetic remineralisation and has a positive effect on early carious lesions. However, better results can be achieved when it is combined with other agents.
Article
Polymer Science
Noor Huda Ismail, Raja Azman Awang, Thirumulu Ponnuraj Kannan, Zuratul Ain Abdul Hamid, Mariati Jaafar
Summary: This study investigated the physical and mechanical properties and cytotoxicity of a dental hybrid composite adhesive cement (DCAC) made from silica rice husk and different amounts of zirconia and alumina. The results showed that the addition of zirconia and alumina improved the physical and mechanical properties of DCAC, and the cytotoxicity was minimal.
Article
Chemistry, Multidisciplinary
Fayez Hussain Niazi, Norhayati Luddin, Masitah Hayati Harun, Arshad Hasan, Thirumulu Ponnuraj Kannan, Suharni Mohamad, Amer Mahmood
Summary: The purpose of this in vivo study was to compare the dentin-pulp complex response following occlusal and cervical restorations in rat molars restored with nano-HA-SiO2-GIC and c-GIC. Histological examination showed that both restorations caused disruption of the odontoblastic layer and moderate inflammation in the pulp area after one week. Nano-HA-SiO2-GIC showed superior properties in terms of tertiary dentin formation. After one month, the odontoblast layer was intact and the pulp tissue recovered, with a few cases of mild-to-moderate inflammation observed in the nano-HA-SiO2-GIC group.
APPLIED SCIENCES-BASEL
(2023)
Article
Medicine, General & Internal
Divashini Vijian, Wan Suriana Wan Ab Rahman, Kannan Thirumulu Ponnuraj, Zefarina Zulkafli, Rosnah Bahar, Norafiza Yasin, Syahzuwan Hassan, Ezalia Esa
Summary: This study aimed to determine the prevalence, haematological and molecular characterisations of alpha-thalassaemia. With a total cohort of 131 patients, the prevalence of alpha-thalassaemia was 48.9%, leaving the remaining 51.1% with potentially undetected alpha gene mutations. Haematological parameters showed significant changes among patients with deletional mutations, but not between patients with nondeletional mutations.
Review
Engineering, Multidisciplinary
Ali Azhar Dawasaz, Rafi Ahmad Togoo, Zuliani Mahmood, Azlina Ahmad, Kannan Thirumulu Ponnuraj
Summary: The objective of this article was to review the different methods of remineralizing human dentine using biomimetic agents. The authors conducted a systematic search and included 39 full-text articles in the review. The findings showed that biomimetic remineralization using NCP analogs, bioactive materials, and natural products significantly improved the extent of remineralization and elastic modulus.
Article
Dentistry, Oral Surgery & Medicine
Imran A. Moheet, Norhayati Luddin, Ismail A. Rahman, Sam'an M. Masudi, Thirumulu P. Kannan, Nik Rozainah N. Abd Ghani
JOURNAL OF INTERNATIONAL ORAL HEALTH
(2019)
Article
Materials Science, Multidisciplinary
Arbaz Sajjad, Wan Zaripah Wan Bakar, Dasmawati Mohamad, Thirumulu Ponnuraj Kannan
AIMS MATERIALS SCIENCE
(2019)
Article
Public, Environmental & Occupational Health
Imran Alam Moheet, Norhayati Luddin, Ismail Ab Rahman, Sam'an Malik Masudi, Thirumulu Ponnuraj Kannan, Nik Rozainah Nik Abd Ghani
