Article
Immunology
Simone Giovannozzi, Jonas Demeulemeester, Rik Schrijvers, Rik Gijsbers
Summary: STAT1 gain-of-function (GOF) leads to primary immunodeficiency with characteristic symptoms. Different mutation-specific mechanisms are identified in vitro. GOF mutants up-regulate an additional set of genes compared to wild-type STAT1 upon IFN gamma stimulation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Jack L. McMurray, Anouk von Borstel, Taher E. Taher, Eleni Syrimi, Graham S. Taylor, Maria Sharif, Jamie Rossjohn, Ester B. M. Remmerswaal, Frederike J. Bemelman, Felipe A. Vieira Braga, Xi Chen, Sarah A. Teichmann, Fiyaz Mohammed, Andrea A. Berry, Kirsten E. Lyke, Kim C. Williamson, Michael J. T. Stubbington, Martin S. Davey, Carrie R. Willcox, Benjamin E. Willcox
Summary: Gamma delta T cells are traditionally considered innate-like lymphocytes, but a new adaptive-like compartment has now been identified. This adaptive subset undergoes pathogen-driven differentiation, similar to conventional CD8(+) T cells. Expansions of specific TCRs are detected exclusively in the effector cells, indicating a specialized response to antigens.
Article
Biochemistry & Molecular Biology
Kaja Blagotinsek Cokan, Ziga Urlep, Miha Moskon, Miha Mraz, Xiang Yi Kong, Winnie Eskild, Damjana Rozman, Peter Juvan, Tadeja Rezen
Summary: Through mouse models, it was found that various genetic models of liver fibrosis exhibit a common transcriptional program and downregulated lipid pathways, while different metabolic subtypes of liver fibrosis were proposed, aiding in improved stratification of other fibrosis-related pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Chengheng Liao, Cherise Ryan Glodowski, Cheng Fan, Juan Liu, Kevin R. Mott, Akash Kaushik, Hieu Vu, Jason W. Locasale, Samuel K. McBrayer, Ralph J. DeBerardinis, Charles M. Perou, Qing Zhang
Summary: Metabolic dysregulation in breast cancer was characterized and divided into two major metabolic groups. Genes strongly correlated with metabolic dysregulation and predicted patient prognosis were identified. Targeting metabolic dysregulation led to effective therapeutic outcomes. This study is of great significance in guiding therapeutic strategies for breast cancer subsets.
Article
Genetics & Heredity
Xianxi Huang, Wenjun Shen, Stefan Veizades, Grace Liang, Nazish Sayed, Patricia K. Nguyen
Summary: This study found differential gene expression in endothelial cells between male and female mice at 3 and 18 months, with older mice showing increased expression of genes related to inflammation. The findings suggest that age plays a role in the development of chronic diseases in endothelial cells.
FRONTIERS IN GENETICS
(2021)
Article
Medicine, General & Internal
Yingying Ding, Haijiang Lin, Xiaoxiao Chen, Bowen Zhu, Xiaohui Xu, Xiaoyi Xu, Weiwei Shen, Meiyang Gao, Na He
Summary: HIV infection and NCDs are connected through metabolic disturbances, with GPL metabolism being the common link between HIV and NCDs, followed by glutamine and glutamate metabolism.
Article
Neurosciences
Muhamed N. H. Eeza, Rico Singer, Corinna Hoefling, Joerg Matysik, Huub J. M. de Groot, Steffen Rossner, A. Alia
Summary: Metabolic profiling and immunohistochemical analysis in Tg2576 mouse model revealed significant metabolic deficits and low expression of core clock protein Bmal1 in SCN, especially in activated astrocytes. Additionally, decreased expression of GAT1 in astrocytes indicates low GABA recycling. The results suggest that redox disbalance and compromised GABA signaling play important roles in connecting neuroinflammation and clock dysfunction in AD.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Multidisciplinary Sciences
Lingzi Li, Janina Freitag, Christian Asbrand, Bogdan Munteanu, Bei-Tzu Wang, Ekaterina Zezina, Michel Didier, Gilbert Thill, Corinne Rocher, Matthias Herrmann, Nadine Biesemann
Summary: This study investigated the metabolic changes during the development of murine arthritis by integrating metabolomics and transcriptomics data. The early change in macrophage pathways, accompanied by oxidative stress, a drop in NAD+ level and induction of glucose transporters, was identified. Additionally, the dysregulation of SIRT1 in human macrophages and synovial tissues of RA patients was confirmed.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Emelia Osman, Anis Safirah Mohammad Zahariluddin, Shalisah Sharip, Zulkarnain Md Idris, Jen Kit Tan
Summary: This study found that both Toxoplasma infection and schizophrenia lead to alterations in purine catabolism. Further research is needed to investigate the specific roles of these metabolites in the pathogenesis of schizophrenia associated with Toxoplasma infection.
Article
Microbiology
Joshua M. Wang, Andrew L. Woodruff, Matthew J. Dunn, Robert J. Fillinger, Richard J. Bennett, Matthew Z. Anderson
Summary: The research on 21 clinical isolates of Candida albicans showed extensive genetic and phenotypic variation, with strain-specific gene expression patterns and linkages between gene expression and phenotypic traits. The study highlights the importance of transcriptional profiling in understanding the genetic basis for phenotypic diversity and identifies new genes and pathways associated with pathogenic processes. These findings underscore the need for personalized treatment strategies based on individual strains of infectious fungi.
Article
Parasitology
Xueyan Hu, Jie Zhao, Junhui Zhao, Ence Yang, Mozhi Jia
Summary: The study found that during the blood stage of the parasite, patients with malaria exhibit activated immune responses, aberrant metabolic processes, and tissue remodeling changes in the liver, providing a systematic outline of liver responses during Plasmodium infection.
PARASITES & VECTORS
(2023)
Article
Cell Biology
Yuanlin He, Qiuzhen Chen, Juncheng Dai, Yiqiang Cui, Chi Zhang, Xidong Wen, Jiazhao Li, Yue Xiao, Xiaoxu Peng, Mingxi Liu, Bin Shen, Jiahao Sha, Zhibin Hu, Jing Li, Wenjie Shu
Summary: The study identified a unified trajectory and regulatory mechanisms of oocyte development in the newborn ovary through RNA-seq of single germ cells. Gene Ontology term enrichment revealed key genes with dynamic changes and identified a series of genes and molecular pathways in follicle formation and development.
Article
Immunology
Panpan Liu, Guixue Hou, Yehong Kuang, Lei Li, Chao Chen, Bei Yan, Wu Zhu, Jie Li, Mingliang Chen, Juan Su, Liang Lin, Xiang Chen, Cong Peng
Summary: This study investigated the alterations of lipids in psoriatic skin lesions and found enrichments of CerP and ceramides. Consensus clustering of lipid signatures classified psoriasis patients into two subtypes, CC1 and CC2, with CC1 subtype showing more severe symptoms. Further research revealed that C1P, which was dramatically enriched in CC1 subtype, facilitated psoriasis-like inflammatory responses. Inhibiting the production of C1P effectively alleviated the inflammation.
CLINICAL IMMUNOLOGY
(2023)
Article
Oncology
Ana Costa, Cecile Thirant, Amira Kramdi, Cecile Pierre-Eugene, Caroline Louis-Brennetot, Orphee Blanchard, Didier Surdez, Nadege Gruel, Eve Lapouble, Gaelle Pierron, Deborah Sitbon, Herve Brisse, Arnaud Gauthier, Paul Freneaux, Mylene Bohec, Virginie Raynal, Sylvain Baulande, Renaud Leclere, Gabriel Champenois, Andre Nicolas, Didier Meseure, Angela Bellini, Aurelien Marabelle, Birgit Geoerger, Fatima Mechta-Grigoriou, Gudrun Schleiermacher, Laurie Menger, Olivier Delattre, Isabelle Janoueix-Lerosey
Summary: This study reveals that the immunocompromised microenvironment of neuroblastoma tumors is characterized by dysfunctional T cells and accumulation of immunosuppressive cells. The study provides a new and valuable data resource to better understand the neuroblastoma ecosystem and suggests novel therapeutic strategies targeting both tumor cells and components of the microenvironment.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Multidisciplinary Sciences
Lin Que, David Lukacsovich, Wenshu Luo, Csaba Foldy
Summary: The study investigated transcriptomic states of morphologically identified parvalbumin interneurons (PV-INs) using single-cell RNA-seq, revealing high transcriptomic similarity among PV-INs with only few genes showing divergent expression. Additionally, PV-INs exhibited a uniform synaptic cell adhesion molecule (CAM) profile, suggesting CAM expression in mature cells does not reflect wiring specificity post-development.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Fangfang Li, Zongliang Liu, Heyuan Sun, Chunmei Li, Wenyan Wang, Liang Ye, Chunhong Yan, Jingwei Tian, Hongbo Wang
ACTA PHARMACEUTICA SINICA B
(2020)
Article
Oncology
Zhong-Yan Hua, Jeanne N. Hansen, Miao He, Shang-Kun Dai, Yoonjung Choi, Melody D. Fulton, Sarah M. Lloyd, Marianna Szemes, Ji Sen, Han-Fei Ding, James M. Angelastro, Xiang Fei, Hui-Ping Li, Chao-Ran Wu, Sheng-Yong Yang, Karim Malik, Xiaomin Bao, Y. George Zheng, Chang-Mei Liu, Nina F. Schor, Zhi-Jie Li, Xing-Guo Li
Article
Nanoscience & Nanotechnology
Ahmet Alptekin, Mohammad B. Khan, Roxan Ara, Mohammad H. Rashid, Fengchong Kong, Mahrima Parvin, Joseph A. Frank, Rajiv Chopra, Krishnan Dhandapani, Ali S. Arbab
Summary: Exosomes have been shown to carry important biomolecules and can be delivered to stroke areas using pulsed focused ultrasound, but the administration of nanobubbles has detrimental effects on brain structures.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2021)
Article
Cell Biology
Yajie Yu, Jane Ding, Shunqin Zhu, Ahmet Alptekin, Zheng Dong, Chunhong Yan, Yunhong Zha, Han-Fei Ding
Summary: Metabolic reprogramming driven by oncogenes like MYC is closely related to growth promotion, but also leads to potential therapeutic metabolic dependencies; MYCN-amplified neuroblastoma promotes pyrimidine nucleotide production by upregulating DHODH and other enzymes, and inhibition of DHODH can effectively suppress proliferation and tumorigenicity of these cancer cells.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Xingyao Li, Daniel Gracilla, Lun Cai, Mingyi Zhang, Xiaolin Yu, Xiaoguang Chen, Junran Zhang, Xiaochun Long, Han-Fei Ding, Chunhong Yan
Summary: ATF3 plays a crucial role in transcriptional activation of the serine synthesis pathway (SSP) under serine deprivation, positively correlates with PHGDH expression, and loss of ATF3 expression impairs cancer cell growth.
Article
Pathology
Zhengwei Ma, Xiaoru Hu, Han-Fei Ding, Ming Zhang, Yuqing Huo, Zheng Dong
Summary: This study is the first to investigate cisplatin-induced nephrotoxicity using snRNA-seq, revealing that RLDC treatment leads to renal dysfunction and pathological changes in mice, as well as transcriptional alterations at the single-cell level in PT cells.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Oncology
Chunwan Lu, Dafeng Yang, John D. Klement, Yolonda L. Colson, Nicholas H. Oberlies, Cedric J. Pearce, Aaron H. Colby, Mark W. Grinstaff, Han-Fei Ding, Huidong Shi, Kebin Liu
Summary: This study reveals the impact of metabolic pathways on the function of tumor-specific CTLs and tumor immune evasion. It identifies the key role of G6PD in CTL activation and discovers the significance of the H3K9me3/G6PD/acetyl-CoA/H3K9ac/Gzmb pathway in epithelioid mesothelioma.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Oncology
Mujibur Khan, Md Mahmudul Hasan, Anne Barnett, Raziye Piranlioglu, Mohammad Rashid, Ahmet Alptekin, Ali Arbab
Summary: A novel coaxial electrospraying technique was developed to produce multi-cancer drug nanocapsules, which showed prolonged and sustained drug release patterns in vitro and in vivo studies. The nanocapsules containing multiple drugs were effective in controlling tumor cell growth and increasing survival rates in animals with breast cancers.
CANCER NANOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chunwan Lu, Dafeng Yang, John D. Klement, Yolonda L. Colson, Nicholas H. Oberlies, Cedric J. Pearce, Aaron H. Colby, Mark W. Grinstaff, Zhuoqi Liu, Huidong Shi, Han-Fei Ding, Kebin Liu
Summary: The role of G6PD in human cancer is not fully understood. Inhibition of H3K9 methylation suppresses aerobic glycolysis and enhances PPP, leading to increased tumor cell apoptosis. Activation of G6PD induces tumor cell death, suppresses tumor growth, and increases mouse survival in vivo.
Article
Medicine, Research & Experimental
Deanna Doughty, Surendra K. Rajpurohit, Amy Trang, Ahmet Alptekin, Ahmet K. Korkaya, Bhagelu R. Achyut, Ali S. Arbab, Jennifer W. Bradford
Summary: This article introduces a new method for enriching and isolating primary microglia from mouse brain tissue, as well as flow cytometry methods using novel cell surface markers to specifically label microglia. The technique provides a large number of cells for studies in human CNS biology and contributes to understanding the crucial role of microglia in brain function and disease mechanisms.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2022)
Review
Immunology
Ahmet Alptekin, Mahrima Parvin, Hasanul I. Chowdhury, Mohammad H. Rashid, Ali S. Arbab
Summary: Exosomes are small extracellular vesicles secreted by cells, capable of altering the microenvironment through interactions with other cells. Engineered and designer exosomes hold great potential for delivering drugs and biologically active molecules to specific sites, opening up new avenues for diagnosis and treatment.
IMMUNOLOGICAL REVIEWS
(2022)
Article
Oncology
Vu N. Bach, Jane Ding, Miranda Yeung, Taylor Conrad, Hussain N. Odeh, Paige Cubberly, Christopher Figy, Han-Fei Ding, Robert Trumbly, Kam C. Yeung
Summary: Breast cancer is the second most common cancer in women worldwide, and the regulation of its metastasis is not well understood. RKIP has been shown to suppress breast cancer metastasis by downregulating elements of the immune system. In addition to its negative regulation of the Raf signaling pathway, RKIP may exert its effects on cancer through modulating various signaling pathways, including those involved in immune response.
Review
Oncology
Mohit Bansal, Anamika Gupta, Han-Fei Ding
Summary: Metabolic reprogramming plays a crucial role in high-risk neuroblastoma and offers potential therapeutic opportunities. This review summarizes the recent progress in understanding neuroblastoma metabolic reprogramming and highlights important areas for future research.
Article
Pharmacology & Pharmacy
Hesam Khodadadi, Lvila Lopes Salles, Ahmet Alptekin, Daniel Mehrabian, Martin Rutkowski, Ali S. Arbab, W. Andrew Yeudall, Jack C. Yu, John C. Morgan, David C. Hess, Kumar Vaibhav, Krishnan M. Dhandapani, Babak Baban
Summary: In this study, the researchers found that inhalation of cannabidiol (CBD) can inhibit the growth of glioblastoma (GBM) tumors and alter the tumor microenvironment (TME). This suggests that CBD could serve as an effective adjunct treatment for GBM.
CANNABIS AND CANNABINOID RESEARCH
(2023)