Article
Biochemistry & Molecular Biology
Hironori Mizutani, Yoshifumi Sato, Masaya Yamazaki, Tatsuya Yoshizawa, Yukio Ando, Mitsuharu Ueda, Kazuya Yamagata
Summary: This study found that the expression of SIRT7 is increased in the brain of Alzheimer's disease patients. Treatment with A beta(42) increased the expression of NOX4, leading to the production of reactive oxygen species (ROS) and apoptosis. Knockdown of SIRT7 inhibited A beta(42)-induced ROS production and apoptosis by suppressing the upregulation of NOX4. These findings suggest that inhibiting SIRT7 may be beneficial in the pathogenesis of AD by regulating ROS production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yoon-Seok Seo, Jung-Min Park, Jae-Hyeong Kim, Moo-Yeol Lee
Summary: Smoking is a significant risk factor for various diseases due to the production of excessive reactive oxygen species (ROS). This review focuses on how cigarette smoke promotes the formation of ROS and provides key mechanisms involved.
Article
Biochemistry & Molecular Biology
Ziting Guo, Jinjin Zhao, Meiping Wang, Song Song, Zongliang Xia
Summary: Recent studies have shown that sulfur dioxide (SO2) plays a positive role in stress defense responses in plants, especially in seed germination. SO2 treatment can significantly promote seed germination and vigor in maize by increasing reactive oxygen species levels and NADPH oxidase activities, leading to elevated alpha-amylase activity and transcripts. These findings suggest that SO2 may function as a signaling molecule in plant growth and development, specifically in seed germination control.
Article
Pharmacology & Pharmacy
Sergejs Zavadskis, Adelheid Weidinger, Dominik Hanetseder, Asmita Banerjee, Cornelia Schneider, Susanne Wolbank, Darja Marolt Presen, Andrey Kozlov
Summary: The study found that the inhibitor DPI has different effects on ROS metabolism and mitochondrial function in various types of cells, such as stem cells and differentiated cells, depending on the cell's energy metabolism. Undifferentiated cells may be a better target for DPI compared to differentiated parenchymal cells.
Article
Biochemistry & Molecular Biology
Marta Massari, Callum R. Nicoll, Sara Marchese, Andrea Mattevi, Maria Laura Mascotti
Summary: This article investigates the phylogenetic analysis of the NADPH oxidase family in eukaryotes and finds that all known members share a common ancestor. Calcium-dependency is identified as the earliest form of NOX regulation, while the protein-protein mode of regulation is a more recent event generated by gene duplication.
Review
Medicine, Research & Experimental
Yawei Bi, Xiao Lei, Ningli Chai, Enqiang Linghu
Summary: NOX4 is upregulated in pancreatic cancer and primarily produces hydrogen peroxide, exhibiting different properties from other NOX family subtypes. Recent studies suggest that NOX4 promotes pancreatic cancer occurrence and development, making it a potential therapeutic target in pancreatic cancer.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Andrew J. Robinson, Sara Davies, Richard L. Darley, Alex Tonks
Summary: In AML patients, over 60% exhibit constitutive activation of NOX2 and increased ROS production. This leads to increased glucose uptake and proliferation in AML cells, as well as changes in carbohydrate metabolism. Metabolomic profiling revealed significant ROS-specific metabolic alterations in sphingolipid metabolism, fatty acid oxidation, purine metabolism, amino acid homeostasis, and glycolysis, providing further evidence of ROS rewiring metabolism in AML.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Qinhong Wang, Rahima Zennadi
Summary: Sickle cell disease is a genetic disorder characterized by oxidative stress in red blood cells, leading to various systemic manifestations.
Article
Biochemistry & Molecular Biology
Ildiko Szanto
Summary: Cancer cells can adapt to hypoxic environments through metabolic rewiring and synthesis of antioxidant molecules. NOX4, a member of the NOX family enzymes, plays a key role in the oncogenic metabolic adaptation of cancer cells by regulating diverse metabolic processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yutong Jin, Brian Dixon, Lyndon Jones, Maud Gorbet
Summary: Tear polymorphonuclear neutrophils (PMNs) invade the ocular surface during sleep, contributing to homeostasis and possessing an arsenal of inflammatory mediators. This study found that tear PMNs exhibit high constitutive ROS production and differential responses to stimuli like PMA, LPS, and fMLP, suggesting their potential role in ocular inflammatory complications involving oxidative stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Misbah Hussain, Wajiha Ikram, Usama Ikram
Summary: Oxidative stress affects the redox state of various target proteins, including tyrosine kinases, mitogen-activated protein kinases, and tyrosine phosphatases. c-Src is a non-receptor tyrosine kinase that can activate NADPH oxidases in response to different stimuli. The interaction between c-Src and Noxs is influenced by cell type and primary messengers. Additionally, c-Src and ROS have a bidirectional relationship in the pathobiology of cardiac fibrosis.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Pharmacology & Pharmacy
Eunice E. To, Jonathan R. Erlich, Felicia Liong, Stella Liong, Raymond Luong, Osezua Oseghale, Mark A. Miles, Paris C. Papagianis, Kylie M. Quinn, Steven Bozinovski, Ross Vlahos, Robert D. Brooks, John J. O'Leary, Doug A. Brooks, Stavros Selemidis
Summary: The combination of an endosome-targeted NOX2 oxidase inhibitor and a mitochondrial ROS scavenger is more effective than monotherapy in reducing inflammation and improving lung pathology in influenza A virus (IAV) infection. This approach also enhances Type I interferon expression and increases IAV-specific CD8(+) T cells in the lungs.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Flavia Rezende, Pedro Felipe Malacarne, Niklas Mueller, Birgit Rathkolb, Martin Hrabe de Angelis, Katrin Schroeder, Ralf P. Brandes
Summary: The study found that Nox4 knockout mice exhibited similar weight loss and sodium sparing function under a low sodium diet, but showed reduced systolic blood pressure, heart-rate ratio, and heart to body weight ratio compared to wild-type mice, indicating that Nox4 plays a role in blood pressure regulation induced by low sodium diets.
Article
Biochemistry & Molecular Biology
Jin Han, Donghwi Park, Ji Young Park, Seungwoo Han
Summary: Increased oxidative stress in OA cartilage leads to extracellular matrix degradation and chondrocyte apoptosis. This study found that inhibiting NOX can decrease ROS production, suppress oxidative stress marker genes and catabolic proteases, and protect against OA by modulating oxidative stress and gene expression.
Review
Immunology
Rizwana Begum, Shilpa Thota, Abubakar Abdulkadir, Gagandeep Kaur, Prathyusha Bagam, Sanjay Batra
Summary: This review provides an overview of the role of reactive oxygen species (ROS) in biological systems and the importance of NOX enzymes in antimicrobial mechanisms and disease pathophysiology. The article emphasizes on the therapeutic strategies to inhibit ROS production and highlights the significance of computational modeling in future NOX-related studies.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chet E. Holterman, Naomi C. Boisvert, Jean-Francois Thibodeau, Eldjonai Kamto, Melica Novakovic, Khaled S. Abd-Elrahman, Stephen S. G. Ferguson, Christopher R. J. Kennedy
ANTIOXIDANTS & REDOX SIGNALING
(2019)
Article
Medicine, Research & Experimental
Jean-Francois Thibodeau, Jean-Christophe Simard, Chet E. Holterman, Amelie Blais, Marie-Pier Cloutier, Thalia Medeiros, Martin Leduc, Brigitte Grouix, Francois A. Leblond, Dylan Burger, Richard L. Hebert, Christopher R. J. Kennedy, Lyne Gagnon
Article
Endocrinology & Metabolism
Jay C. Jha, Aozhi Dai, Chet E. Holterman, Mark E. Cooper, Rhian M. Touyz, Chris R. Kennedy, Karin A. M. Jandeleit-Dahm
Article
Multidisciplinary Sciences
Alex A. Gutsol, Paula Blanco, Svetlana I. Samokhina, Sergey A. Afanasiev, Chris R. J. Kennedy, Sergey V. Popov, Kevin D. Burns
Article
Medicine, Research & Experimental
Rania Nasrallah, Joseph Zimpelmann, Susan J. Robertson, Jamie Ghossein, Jean-Francois Thibodeau, C. R. J. Kennedy, Alex Gutsol, Fengxia Xiao, Dylan Burger, Kevin D. Burns, Richard L. Hebert
LABORATORY INVESTIGATION
(2020)
Article
Biochemistry & Molecular Biology
Karim Bouzakri, Christelle Veyrat-Durebex, Chet Holterman, Caroline Arous, Charlotte Barbieux, Domenico Bosco, Jordi Altirriba, Mohamed Alibashe, Benjamin B. Tournier, Jenny E. Gunton, Sarah Mouche, William Bietiger, Alexis Forterre, Thierry Berney, Michel Pinget, Gerhard Christofori, Christopher Kennedy, Ildiko Szanto
ANTIOXIDANTS & REDOX SIGNALING
(2020)
Article
Peripheral Vascular Disease
Devy Deliyanti, Saeed F. Alrashdi, Rhian M. Touyz, Christopher R. Kennedy, Jay C. Jha, Mark E. Cooper, Karin A. Jandeleit-Dahm, Jennifer L. Wilkinson-Berka
Article
Neurosciences
Julie Ouellette, Xavier Toussay, Cesar H. Comin, Luciano da F. Costa, Mirabelle Ho, Maria Lacalle-Aurioles, Moises Freitas-Andrade, Qing Yan Liu, Sonia Leclerc, Youlian Pan, Ziying Liu, Jean-Francois Thibodeau, Melissa Yin, Micael Carrier, Cameron J. Morse, Peter Van Dyken, Christopher J. Bergin, Sylvain Baillet, Christopher R. Kennedy, Marie-Eve Tremblay, Yannick D. Benoit, William L. Stanford, Dylan Burger, Duncan J. Stewart, Baptiste Lacoste
NATURE NEUROSCIENCE
(2020)
Article
Multidisciplinary Sciences
Jean-Christophe Simard, Jean-Francois Thibodeau, Martin Leduc, Mikael Tremblay, Alexandre Laverdure, Francois Sarra-Bournet, William Gagnon, Jugurtha Ouboudinar, Liette Gervais, Alexandra Felton, Sylvie Letourneau, Lilianne Geerts, Marie-Pier Cloutier, Kathy Hince, Ramon Corpuz, Alexandra Blais, Vanessa Marques Quintela, Jean-Simon Duceppe, Shaun D. Abbott, Amelie Blais, Boulos Zacharie, Pierre Laurin, Steven R. Laplante, Christopher R. J. Kennedy, Richard L. Hebert, Francois A. Leblond, Brigitte Grouix, Lyne Gagnon
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Alex A. Gutsol, Paula Blanco, Taben M. Hale, Jean-Francois Thibodeau, Chet E. Holterman, Rania Nasrallah, Jose W. N. Correa, Sergey A. Afanasiev, Rhian M. Touyz, Chris R. J. Kennedy, Dylan Burger, Richard L. Hebert, Kevin D. Burns
Summary: Current research on hypertension often uses animal models, but these models do not fully replicate the glomerular injuries seen in humans. This study evaluated four hypertensive models and found that only the clipped kidneys in 2K1C rats showed similar glomerulopathy to humans. To make animal studies more relevant, the criteria and classifications established in human pathology should be used for analysis and improvement.
Article
Endocrinology & Metabolism
Jay C. Jha, Aozhi Dai, Jessica Garzarella, Amelia Charlton, Sofia Urner, Jakob A. Ostergaard, Jun Okabe, Chet E. Holterman, Alison Skene, David A. Power, Elif I. Ekinci, Melinda T. Coughlan, Harald H. H. W. Schmidt, Mark E. Cooper, Rhian M. Touyz, Chris R. Kennedy, Karin Jandeleit-Dahm
Summary: Excessive production of renal reactive oxygen species (ROS) plays a major role in diabetic kidney disease (DKD). This study demonstrates that NADPH oxidase 5 (NOX5) acts as a predominant pro-oxidant enzyme in DKD, independent of the previously characterized NOX4 pathway. The overexpression of NOX5 in DKD exacerbates kidney damage through enhanced ROS formation and modulation of key molecules involved in inflammation, fibrosis, and redox balance. Furthermore, the only available NOX inhibitor, GKT137831, is found to be ineffective in the presence of NOX5 expression in diabetes. These findings highlight the superior targeting potential of NOX5 as compared to other NOX isoforms like NOX4 in human DKD.
Letter
Oncology
Chet E. Holterman, Robert L. Myette, Dylan Burger, Christopher RJ. Kennedy
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Neurosciences
Daniel N. N. El Kodsi, Jacqueline M. M. Tokarew, Rajib Sengupta, Nathalie A. A. Lengacher, Ajanta Chatterji, Angela P. P. Nguyen, Heather Boston, Qiubo Jiang, Carina Palmberg, Chantal Pileggi, Chet E. E. Holterman, Bojan Shutinoski, Juan Li, Travis K. Fehr, Matthew J. LaVoie, Rajiv R. R. Ratan, Gary S. S. Shaw, Masashi Takanashi, Nobutaka Hattori, Christopher R. R. Kennedy, Mary-Ellen Harper, Arne Holmgren, Julianna J. J. Tomlinson, Michael G. G. Schlossmacher
Summary: The expression of PRKN gene is associated with young-onset Parkinson's disease and plays a role in redox homeostasis. In a mouse model, the loss of PRKN gene combined with Sod2 haploinsufficiency led to increased oxidative stress and altered redox changes in the cytosol. Further research revealed that PRKN gene is involved in the recycling of glutathione, affecting the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) in cells.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Akram Abolbaghaei, Maddison Turner, Jean-Francois Thibodeau, Chet E. E. Holterman, Christopher R. J. Kennedy, Dylan Burger
Summary: Hypertension and diabetes induce vascular injury through unknown processes. Changes in extracellular vesicle composition may provide new insights. This study analyzed the protein composition of circulating extracellular vesicles from hypertensive, diabetic, and healthy mice, identifying differentially expressed proteins and potential pathways involved in vascular injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Physiology
Naomi C. Boisvert, Chet E. Holterman, Alexey Gutsol, Josee Coulombe, Wanling Pan, R. Todd Alexander, Douglas A. Gray, Chris R. Kennedy
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2018)