Article
Medicine, Research & Experimental
Janaina Esmeraldo Rocha, Thiago Sampaio de Freitas, Jayze da Cunha Xavier, Raimundo Luiz Silva Pereira, Francisco Nascimento Pereira Junior, Carlos Emidio Sampaio Nogueira, Marcia Machado Marinho, Paulo Nogueira Bandeira, Mateus Rodrigues de Oliveira, Emmanuel Silva Marinho, Alexandre Magno Rodrigues Teixeira, Helcio Silva dos Santos, Henrique Douglas Melo Coutinho
Summary: A large number of infections worldwide are caused by multi-resistant bacteria, resulting in approximately 700,000 deaths per year. Chalcones, a class of compounds known for their broad biological activities, including antimicrobial and anticancer properties, are being studied for their potential to combat microbial drug resistance.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Review
Biochemical Research Methods
Shalni Kumar, Jeff Hasty
Summary: Synthetic biology needs to focus on stabilizing and containing the function of engineered microbes in target environments. Recent advancements include layered sensing circuits, genetic contexts less susceptible to mutation, burden and resource control circuits, and methods for reducing population variability. These tools enhance the robustness and stability of complex microbial systems for real world deployment.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Debora Feitosa Muniz, Cristina Rodrigues dos Santos Barbosa, Irwin Rose Alencar de Menezes, Erlanio Oliveira de Sousa, Raimundo Luiz Silva Pereira, Joao Tavares Calixto Junior, Pedro Silvino Pereira, Yedda M. L. S. de Matos, Roger H. S. da Costa, Cicera Datiane de Morais Oliveira-Tintino, Henrique Douglas Melo Coutinho, Jose Maria Barbosa Filho, Gabriela Ribeiro de Sousa, Jaime Ribeiro Filho, Jose Pinto Siqueira-Junior, Saulo Relison Tintino
Summary: This study evaluated the antibacterial activity of eugenol and its derivatives against Staphylococcus aureus NorA efflux pump, showing synergistic effects with some compounds. The findings suggest the potential use of eugenol derivatives in antibacterial drug development, particularly in strains carrying the NorA pump.
Article
Biotechnology & Applied Microbiology
Fangxue Ma, Hong Zhou, Zhiqiang Yang, Chao Wang, Yanan An, Lihui Ni, Mingyuan Liu, Yang Wang, Lu Yu
Summary: This study developed a modified in vitro M. tuberculosis biofilm model with shorter culture time and used Illumina RNA-seq technology to identify the global gene expression profile of M. tuberculosis biofilms. The results showed that genes involved in various metabolic pathways are differentially expressed in biofilm cells compared to planktonic cells, providing insights into potential target genes and strategies for M. tuberculosis biofilm inhibition.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Morgane Choquet, Elodie Lohou, Etienne Pair, Pascal Sonnet, Catherine Mullie
Summary: The study evaluated the overexpression of efflux pumps in ciprofloxacin-resistant Acinetobacter baumannii strains, with most genes belonging to the RND family. Synthesis of 1-(1-naphthylmethyl)piperazine analogs as potential efflux pump inhibitors showed that quinoline and pyridine analogs were more effective, with stereochemistry playing a key role in inhibitory activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Veterinary Sciences
Yuru Guo, Chengcheng Huang, Hongyu Su, Zehui Zhang, Menghan Chen, Ruxia Wang, Dexian Zhang, Luyao Zhang, Mingchun Liu
Summary: This study found that the natural flavonoid luteolin can inhibit the MsrA efflux pump in T. pyogenes isolates, thereby increasing susceptibility to antimicrobial drugs. Luteolin inhibits MsrA activity and ATPase activity, leading to a lack of energy source in T. pyogenes and providing a potential innovative treatment for bacterial infections.
VETERINARY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Julalak C. Ontong, Nwabor F. Ozioma, Supayang P. Voravuthikunchai, Sarunyou Chusri
Summary: The study demonstrated that combinations of colistin with other antibiotics were effective in inhibiting colistin-resistant K. pneumoniae isolates, providing a new direction and potential for the treatment of multidrug-resistant pathogens.
Article
Chemistry, Multidisciplinary
Thien-Vy Phan, Phuong Nguyen Hoai Huynh, Vu-Thuy-Vy Nguyen, Thanh-Phuc Nguyen, Thanh-Thao Vu, Cam-Van Thi Vo, Minh-Tri Le, Bao Gia Dang Nguyen, Phuong Truong, Khac-Minh Thai
Summary: By multistep screening, novel asymmetric imidazole-4,5-dicarboxamide derivatives targeting the drug-resistant pump AcrAB-TolC in E. coli were identified. The synthesized compounds with the morpholine moiety showed inhibitory activity against AcrB in vitro and exhibited promising pharmacokinetic properties.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Microbiology
Xun Gao, Chengzhen Wang, Luchao Lv, Xiaotong He, Zhongpeng Cai, Wanyun He, Tong Li, Jian-Hua Liu
Summary: A newly identified transferable plasmid-mediated tigecycline resistance gene cluster, tmexCD4-toprJ4, was found in Klebsiella quasipneumoniae and Enterobacter roggenkampii isolated from chicken meat and environmental samples. The gene cluster can increase bacterial resistance to tigecycline and is different from other variants. It can also synergistically reduce susceptibility to tigecycline in combination with other genes.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Ruichao Li, Kai Peng, Xia Xiao, Yuan Liu, Daxin Peng, Zhiqiang Wang
Summary: This study provides the first report of co-occurrence of bla(VIM-2) and tmexCD1-toprJ1 in one plasmid in P. putida. The emergence of plasmid-mediated carbapenem and tigecycline resistance genes in P. putida from migratory birds highlights the importance of surveillance of novel mobile resistance genes in migratory birds.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Review
Microbiology
Yinhuan Ding, Jingchen Hao, Weijia Xiao, Caihong Ye, Xue Xiao, Chunxia Jian, Min Tang, Guangrong Li, Jinbo Liu, Zhangrui Zeng
Summary: The article "The efficacy of Colistin-resistant strains and the mechanisms of multidrug resistance" discusses the contribution of efflux pumps and their related transcriptional regulators to Colistin resistance, as well as the reverse effect of efflux pump inhibitors. Previous studies have shown a complex regulatory relationship between efflux pumps and their transcriptional regulators, as well as LPS synthesis, transport, and modification. CCCP, NMP, and PA & beta;N can reverse Colistin resistance, highlighting the role of efflux pumps and their potential as adjuvant development. The contribution of efflux pumps to Colistin resistance may also be related to specific genetic backgrounds.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Robert J. Nims, Lara Pferdehirt, Noelani B. Ho, Alireza Savadipour, Jeremiah Lorentz, Sima Sohi, Jordan Kassab, Alison K. Ross, Christopher J. O'Conor, Wolfgang B. Liedtke, Bo Zhang, Amy L. McNulty, Farshid Guilak
Summary: This study presents a novel approach using synthetic biology and tissue engineering to develop a mechanically responsive bioartificial tissue that produces therapeutic biologic drug in response to mechanical loading. By deconstructing signaling networks induced by TRPV4 activation, synthetic TRPV4-responsive genetic circuits were created in chondrocytes to produce anti-inflammatory drug in response to mechanical loading, protecting tissues from inflammatory degradation.
Article
Mathematics, Interdisciplinary Applications
Juan Ramon Melendez-Alvarez, Rong Zhang, Xiao-Jun Tian
Summary: This study developed a theoretical framework to examine the dynamics of synthetic gene circuits by considering both growth feedback and resource competition. The results suggest a cooperative behavior between resource-competing gene circuits under growth feedback. Cooperation or competition is non-monotonically determined by the metabolic burden threshold. These findings show that coupling growth feedback and resource competition plays a crucial role in the dynamics of the host-circuit system, and understanding its effects helps control unexpected gene expression behaviors.
CHAOS SOLITONS & FRACTALS
(2023)
Article
Biochemical Research Methods
Ayoub Lasri, Vahid Shahrezaei, Marc Sturrock
Summary: This study introduces Biomodelling.jl, a tool that uses multiscale modeling to generate synthetic scRNA-seq data and evaluate the performance of gene regulatory network inference methods.
BMC BIOINFORMATICS
(2022)
Article
Multidisciplinary Sciences
Saba Kabir, Hasan Ejaz, Syed Zajif Hussain, Muhammad Asif Rasheed, Kashaf Junaid, Abdul Rehman
Summary: Genotypic resistance of bacterial strain to anti-tuberculosis drugs was determined using Line probe assay. The toxic effect of tellurite on Mycobacterium tuberculosis growth was observed at different concentrations through SEM and EDX analysis. RT-PCR was used to measure the impact on the efflux pump gene. XDR-TB strain showed sensitivity to tellurite, resulting in delayed growth and morphological changes. The exact mechanism of action needs further investigation.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Editorial Material
Biochemistry & Molecular Biology
Daniel A. Charlebois, Gabor Balazsi
MOLECULAR SYSTEMS BIOLOGY
(2016)
Article
Multidisciplinary Sciences
Rinki Chauhan, Janani Ravi, Pratik Datta, Tianlong Chen, Dirk Schnappinger, Kevin E. Bassler, Gabor Balazsi, Maria Laura Gennaro
NATURE COMMUNICATIONS
(2016)
Article
Microbiology
Qiuyan Shao, Jimmy T. Trinh, Colby S. McIntosh, Brita Christenson, Gabor Balazsi, Lanying Zeng
Article
Multidisciplinary Sciences
Jimmy T. Trinh, Tamas Szekely, Qiuyan Shao, Gabor Balazsi, Lanying Zeng
NATURE COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Zoltan Bodi, Zoltan Farkas, Dmitry Nevozhay, Dorottya Kalapis, Viktoria Lazar, Balint Csorgo, Akos Nyerges, Bela Szamecz, Gergely Fekete, Balazs Papp, Hugo Araujo, Jose L. Oliveira, Gabriela Moura, Manuel A. S. Santos, Tamas Szekely, Gabor Balazsi, Csaba Pal
Article
Microbiology
Tejas Bouklas, Luz Alonso-Crisostomo, Tamas Szekely, Elizabeth Diago-Navarro, Erika P. Orner, Kalie Smith, Mansa A. Munshi, Maurizio Del Poeta, Gabor Balazsi, Bettina C. Fries
Article
Multidisciplinary Sciences
Daniel A. Charlebois, Kevin Hauser, Sylvia Marshall, Gabor Balazsi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Kevin S. Farquhar, Daniel A. Charlebois, Mariola Szenk, Joseph Cohen, Dmitry Nevozhay, Gabor Balazsi
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
Kevin S. Farquhar, Samira Rasouli Koohi, Daniel A. Charlebois
Summary: Non-genetic mechanisms can contribute to antimicrobial resistance and interact with genetic mechanisms to enhance pathogen resistance. Cellular heterogeneity and gene expression fluctuations play a role in drug resistance at different timescales.
Article
Biochemistry & Molecular Biology
Joshua D. Guthrie, Daniel A. Charlebois
Summary: This study examines the impact of non-genetic resistance on the evolution of genetic drug resistance. The findings suggest that non-genetic resistance promotes cell survival during drug treatment but hinders the development of genetic resistance. Competition within the population has significant effects on resistance mutation fixation time and population survival.
Article
Biochemistry & Molecular Biology
Samira Rasouli Koohi, Shamanth A. Shankarnarayan, Clare Maristela Galon, Daniel A. Charlebois
Summary: Antimicrobial resistance, particularly in the fungal pathogen C. auris, is a major global health crisis. This study focuses on the antifungal tolerance in C. auris and its contribution to treatment failure. The findings suggest that C. auris is tolerant to various antifungal drugs and combining different drugs with the adjuvant chloroquine may help reduce or eliminate tolerance and resistance in patient-derived isolates. Overall, this study emphasizes the importance of addressing tolerance in C. auris infections and exploring strategies to improve treatment outcomes.
Article
Mathematical & Computational Biology
Daniel A. Charlebois
Summary: Predicting evolution is a fundamental problem in biology, particularly in treating antimicrobial resistance, a complex system-level phenomenon. This perspective article explores the limits, predictability, and repeatability of microevolutionary processes, and discusses the opportunities and challenges for predicting antimicrobial resistance in the context of systems biology. Recent research suggests that a systems biology approach integrating quantitative models and multiscale data can predict the evolution of antimicrobial resistance.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2023)
Article
Biophysics
Michael G. Cortes, Jimmy T. Trinh, Lanying Zeng, Gabor Balazsi
BIOPHYSICAL JOURNAL
(2017)
Article
Biophysics
Taylor Firman, Gabor Balazsi, Kingshuk Ghosh
BIOPHYSICAL JOURNAL
(2017)