Review
Polymer Science
Gamal Abdel Nasser Atia, Hany K. Shalaby, Mehrukh Zehravi, Mohamed Mohamady Ghobashy, Hager Abdel Nasser Attia, Zubair Ahmad, Farhat S. Khan, Abhijit Dey, Nobendu Mukerjee, Athanasios Alexiou, Md. Habibur Rahman, Joanna Klepacka, Agnieszka Najda
Summary: This article examined and summarized the current developments in chitosan-based materials, focusing on the modification of chitosan and its use in periodontal regeneration. The study critically reviewed various preparation and modification techniques to enhance chitosan performance and discussed the uses of chitosan-based products in depth.
Article
Chemistry, Multidisciplinary
Ju An Park, Yejin Youm, Hwa-Rim Lee, Yongwoo Lee, Sarah L. Barron, Taejeong Kwak, Gyu Tae Park, Young-Cheol Song, Roisin M. Owens, Jae Ho Kim, Sungjune Jung
Summary: A cell sheet transfer method from a culture surface to a target tissue is introduced in this study. UV-treated parylene, a commercially available flexible material, is used as the culture surface to provide adequate cell adhesiveness for both cell attachment during culture and efficient transfer. Various cell types are successfully transferred using this versatile cell-transfer process. The mechanism of interfacial cell migration between surfaces with different adhesion preferences is observed, and the re-establishment and reversal of cell polarity after transfer are identified. The unique capabilities of 3D multilayer stacking, freeform design, and curved surface application are demonstrated. The therapeutic potential of the cell-sheet delivery system is demonstrated through experiments on cutaneous wound healing and skin-tissue regeneration in mice models.
ADVANCED MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Zeqing Zhao, Jin Liu, Michael D. Weir, Abraham Schneider, Tao Ma, Thomas W. Oates, Hockin H. K. Xu, Ke Zhang, Yuxing Bai
Summary: This review article presents the recent advances in PDLSC-based bone regeneration and periodontal tissue regeneration. By combining PDLSCs with various materials and scaffolds, as well as applying different osteoinductive agents and strategies, PDLSCs demonstrate the potential for bone and periodontal tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Martin Philipp Dieterle, Thorsten Steinberg, Pascal Tomakidi, Jiri Nohava, Kirstin Vach, Simon Daniel Schulz, Elmar Hellwig, Susanne Proksch
Summary: This study developed a new nonwoven scaffold for periodontal tissue engineering. Nonwovens of 16% gelatin/5% hydroxyapatite with additional porosity were produced by electrospinning and in situ cross-linking. The results showed that the scaffold efficiently supported the adhesion and survival of human mesenchymal stem cells (hMSCs) and periodontal ligament fibroblasts (PDLFs), indicating its potential applications.
Review
Chemistry, Multidisciplinary
Ilaria Roato, Beatrice Masante, Giovanni Putame, Diana Massai, Federico Mussano
Summary: This review provides an overview of recent advancements in periodontal tissue engineering, focusing on the use of PDLSCs and 3D scaffolds. The findings demonstrate the potential of these methods for innovative therapies in the future.
Review
Chemistry, Multidisciplinary
Mafalda S. Santos, Marta S. Carvalho, Joao C. Silva
Summary: Periodontitis is an inflammatory infection caused by bacterial plaque accumulation. Current treatments lack bioactive signals for tissue repair and regeneration. Electrospun nanofibers have shown promising results for periodontal regeneration due to their ability to mimic the natural extracellular matrix. This review provides an overview of the current state of electrospun nanofibrous scaffolds in periodontal regeneration strategies.
Review
Dentistry, Oral Surgery & Medicine
Fabiana Divina Magalhaes, Giovanna Sarra, Giovanna Lopes Carvalho, Ana Clara Fagundes Pedroni, Marcia Martins Marques, Leandro Chambrone, Thais Gimenez, Maria Stella Moreira
Summary: The review of 33 studies indicates that cell sheets formed by mesenchymal stem cells derived from dental tissues have great potential for periodontal tissue regeneration in animal models, but the regeneration process is not always complete.
ARCHIVES OF ORAL BIOLOGY
(2021)
Review
Polymer Science
Maoxue Li, Jiaxi Lv, Yi Yang, Guoping Cheng, Shujuan Guo, Chengcheng Liu, Yi Ding
Summary: Hydrogel, a functional polymer material, has high biocompatibility and slow release properties. It provides suitable attachment sites and growth environments for periodontal cells, playing a significant role in periodontal tissue engineering.
Article
Biochemistry & Molecular Biology
Lena Katharina Mueller-Heupt, Nadine Wiesmann-Imilowski, Sofia Schroeder, Jonathan Gross, Pablo Cores Ziskoven, Philipp Bani, Peer Wolfgang Kaemmerer, Eik Schiegnitz, Anja Eckelt, John Eckelt, Ulrike Ritz, Till Opatz, Bilal Al-Nawas, Christopher Synatschke, James Deschner
Summary: Periodontitis is a chronic inflammatory disease that leads to tooth loss and significantly impacts global health. A hyaluronic acid-based dispersion with controlled oxygen delivery shows potential as a treatment for periodontitis. In vitro and in vivo studies demonstrate the biocompatibility, antimicrobial activity against Porphyromonas gingivalis, and angiogenesis enhancement of the developed oxygen-releasing dispersion. This suggests the potential of oxygen-releasing biomaterials for periodontal tissue regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell & Tissue Engineering
Xiuting Wang, Jinlong Chen, Weidong Tian
Summary: This article reviews the progress of stem cell-based tissue engineering and advanced strategies for endogenous regeneration in periodontal regeneration, including techniques such as utilizing cell aggregates to retain extracellular matrix and promoting regeneration through specific scaffold structures.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Polymer Science
Naiwen Tan, Maja Sabalic-Schoener, Linh Nguyen, Francesco D'Aiuto
Summary: Injectable biomaterials have great potential in dental tissue regeneration. This study successfully prepared an injectable thermosensitive beta-TCP and chitosan hydrogel, and demonstrated its feasibility and biocompatibility in periodontal tissue regeneration.
Article
Biophysics
Zhiwei Jiang, Jin He, Xueting Wang, Danji Zhu, Na Li, Lingfei Ren, Guoli Yang
Summary: Nanomaterial-based cell sheet technology has significant potential in regenerative medicine and tissue engineering. Different types of nanomaterials have been used to harvest cell sheets, categorized by mechanisms such as light-induced, thermo-responsive, magnetic-controlled, and ROS-induced. Studies have demonstrated successful outcomes in the regeneration of bone, skeletal muscle, cardiac tissue, tendon, angiogenesis, and osseointegration. However, challenges remain in terms of comprehensive preparation, safety, and cell quality.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Cell & Tissue Engineering
Che-Chang Tu, Nai-Chen Cheng, Jiashing Yu, Yi-Xuan Pan, Wei-Chiu Tai, Yin-Chuan Chen, Po-Chun Chang
Summary: This study evaluates the potential of self-assembled adipose-derived stem cell spheroids (ADsp) in microbial transglutaminase cross-linked gelatin hydrogel (mTG) for treating diabetic periodontal wounds and craniofacial defects. The results show that osteogenically pretreated ADsp-mTG can promote the regeneration of diabetic osseous defects. This suggests that ADsp-mTG can facilitate diabetic periodontal wound healing and craniofacial osseous defect regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Biochemistry & Molecular Biology
Chao Liang, Li Liao, Weidong Tian
Summary: Decellularized extracellular matrix (dECM) promotes stem cell proliferation, migration, adhesion, and differentiation, making it a promising biomaterial for periodontal tissue engineering. It effectively preserves ECM components as they are in native tissue, providing ideal cues for regeneration and repair. Different sources of dECM have different advantages in promoting periodontal tissue regeneration. Various methods have been developed to improve the mechanical strength of dECM, such as functionalized scaffolds and crosslinked soluble dECM. dECM has been successful in periodontal regeneration therapies and holds potential for future applications.
Review
Cell & Tissue Engineering
Dehua Chang, Xiaotong Yang, Siyang Fan, Taibing Fan, Mingkui Zhang, Minoru Ono
Summary: Tissue engineering combines cell biology and material science to construct tissues or organs for disease modeling, drug testing, and regenerative medicine. Cell sheet is a newly developed scaffold-free tissue engineering technology with great application potential. This review summarizes recent progress and future possibilities in preclinical research and clinical applications of cell sheets fabricated from different cell types for cardiac tissue repair, as well as the manufacturing strategies and promising application potential of 3D cell-dense tissue constructed from cell sheets. The mechanisms of mesenchymal stem cell (MSC) sheets in preventing myocardial ischemia and left ventricle remodeling are emphasized, and safety and effectiveness concerns of engineered tissues compared to other types of cell sheets and 3D cardiac tissues are discussed.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Yang Yu, Yuan Yin, Rui-Xin Wu, Xiao-Tao He, Xi-Yu Zhang, Fa-Ming Chen
CELL PROLIFERATION
(2017)
Article
Cell Biology
Xiao-Tao He, Xuan Li, Yuan Yin, Rui-Xin Wu, Xin-Yue Xu, Fa-Ming Chen
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2018)
Article
Cell & Tissue Engineering
Bei-Min Tian, Rui-Xin Wu, Chun-Sheng Bi, Xiao-Tao He, Yuan Yin, Fa-Ming Chen
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2018)
Article
Engineering, Biomedical
Xiao-Tao He, Rui-Xin Wu, Xin-Yue Xu, Jia Wang, Yuan Yin, Fa-Ming Chen
ACTA BIOMATERIALIA
(2018)
Correction
Cell & Tissue Engineering
Fa-Ming Chen, Li-Na Gao, Bei-Min Tian, Xi-Yu Zhang, Yong-Jie Zhang, Guang-Ying Dong, Hong Lu, Qing Chu, Jie Xu, Yang Yu, Rui-Xin Wu, Yuan Yin, Songtao Shi, Yan Jin
STEM CELL RESEARCH & THERAPY
(2018)
Article
Materials Science, Biomaterials
Rui-Xin Wu, Xiao-Tao He, Jin-Hao Zhu, Yuan Yin, Xuan Li, Xiaohua Liu, Fa-Ming Chen
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2019)
Article
Engineering, Biomedical
Xiao-Tao He, Xuan Li, Yu Xia, Yuan Yin, Rui-Xin Wu, Hai-Hua Sun, Fa-Ming Chen
ACTA BIOMATERIALIA
(2019)
Article
Materials Science, Multidisciplinary
Yuan Yin, Xiao-Tao He, Jia Wang, Rui-Xin Wu, Xin-Yue Xu, Yong-Long Hong, Bei-Min Tian, Fa-Ming Chen
APPLIED MATERIALS TODAY
(2020)
Article
Cell & Tissue Engineering
Huan Zhou, Xuan Li, Yuan Yin, Xiao-Tao He, Ying An, Bei-Min Tian, Yong-Long Hong, Li-An Wu, Fa-Ming Chen
STEM CELL RESEARCH & THERAPY
(2020)
Article
Cell & Tissue Engineering
Yi-Zhou Tan, Xin-Yue Xu, Ji-Min Dai, Yuan Yin, Xiao-Tao He, Yi-Lin Zhang, Tian-Xiao Zhu, Ying An, Bei-Min Tian, Fa-Ming Chen
Summary: The study showed that long-term ex vivo expansion can lead to cellular senescence and impaired autophagy, and melatonin can induce cellular rejuvenation by restoring autophagy, potentially through the PI3K/AKT/mTOR signaling pathway.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Genetics & Heredity
Zhi-Bang Li, Hui-Qi Yang, Kun Li, Yuan Yin, Su-Su Feng, Shao-Hua Ge, Yang Yu
Summary: This study comprehensively analyzed the possible regulatory mechanisms of short-term hypoxia on the biological functions of healthy and inflammatory PDLSCs using RNA sequencing. The results showed that DEGs under hypoxic conditions were more enriched in cell cycle and immune pathways, while DEGs under healthy conditions were more enriched in the TGF-beta pathway. Protein-protein interaction network analysis identified 15 hub genes involved in glycolysis, cellular response to hypoxia, cell differentiation, and immune system processes. Additionally, hypoxia significantly affected the expression of genes associated with proliferation, differentiation, migration, apoptosis, and immunoregulation.
FRONTIERS IN GENETICS
(2022)
Correction
Engineering, Biomedical
Xiao-Tao He, Xuan Li, Yu Xia, Yuan Yin, Rui-Xin Wu, Hai-Hua Sun, Fa-Ming Chen
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Yuan Yin, Bei-Min Tian, Xuan Li, Yao-Cheng Yu, Dao-Kun Deng, Li-Juan Sun, Hong-Lei Qu, Rui-Xin Wu, Xin-Yue Xu, Hai-Hua Sun, Ying An, Xiao-Tao He, Fa-Ming Chen
Summary: This study investigates the compromised osteogenic potential of periodontal ligament stem cells (PDLSCs) under inflammatory conditions and explores the underlying mechanism. The researchers found that the disruption of PDLSC autophagy under inflammatory conditions was associated with dysfunction of the cellular autophagy-lysosome system. Furthermore, they demonstrated that treatment with gold nanoparticles (Au NP) could rescue the osteogenic potential of inflamed PDLSCs by restoring the compromised autophagy-lysosome system. This study highlights the importance of the autophagy-lysosome system in cellular osteogenesis under inflammatory conditions and suggests the potential use of nanomaterials for rescuing inflammation-induced cell dysfunction and aiding tissue regeneration.
Article
Pharmacology & Pharmacy
Xiao-Tao He, Jia Wang, Xuan Li, Yuan Yin, Hai-Hua Sun, Fa-Ming Chen
ADVANCED THERAPEUTICS
(2019)
Article
Materials Science, Biomaterials
Jia Wang, Xiao-Tao He, Xin-Yue Xu, Yuan Yin, Xuan Li, Chun-Sheng Bi, Yong-Long Hong, Fa-Ming Chen
JOURNAL OF MATERIALS CHEMISTRY B
(2019)