Article
Oncology
Jian-xin Yin, Wen-wei Hu, Hua Gu, Jian-min Fang
Summary: Early diagnosis of non-small cell lung cancer by detecting circulating tumor DNA is crucial in curtailing cancer-related deaths. Combined assay results and post-operative new ctDNA mutation status are promising prognostic indicators in NSCLC patients.
Article
Biochemistry & Molecular Biology
Begona Jimenez-Rodriguez, Alfonso Alba-Bernal, Esperanza Lopez-Lopez, Maria Elena Quiros-Ortega, Guillermo Carbajosa, Alicia Garrido-Aranda, Martina Alvarez, Ana Godoy-Ortiz, Maria Isabel Queipo-Ortuno, Luis Vicioso, Gema Diaz-Cordoba, Maria Dunia Roldan-Diaz, Jesus Velasco-Suelto, Cristina Hernando, Begona Bermejo, Ana Julve-Parreno, Ana Lluch, Javier Pascual, Inaki Comino-Mendez, Emilio Alba
Summary: A novel ultrasensitive pipeline for detecting circulating tumor DNA (ctDNA) in localized breast cancer patients was developed. There was a 29.50% mutation concordance between tumor tissue and plasma, and 8 patients had plasma-only mutations. The study also revealed associations between ctDNA characteristics and tumor grade, relapse risk, and hormone receptor-negative tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Lisa S. M. Hofste, Maartje J. Geerlings, Daniel von Rhein, Heidi Rutten, Helen A. Westenberg, Marjan M. Weiss, Christian Gilissen, Tom Hofste, Rachel S. van der Post, Bastiaan R. Klarenbeek, Johannes H. W. de Wilt, Marjolijn J. L. Ligtenberg
Summary: This study evaluated the role of circulating tumor DNA (ctDNA) in assessing disease after neoadjuvant therapy and surgery to optimize treatment choices. The results showed that the detection of ctDNA after neoadjuvant therapy and surgery was associated with poor progression-free survival. This suggests that the detection of ctDNA may be helpful in predicting treatment response and prognosis in patients with early-stage and locally advanced rectal cancer.
Article
Oncology
Lingling Wan, Qingyi Liu, Di Liang, Yongdong Guo, Guangjie Liu, Jinxia Ren, Yutong He, Baoen Shan
Summary: This study identified common gene mutations and potential diagnostic markers for early lung cancer through CTC next-generation sequencing and metabolomics analysis. These findings suggest new tumor markers for early lung cancer diagnosis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Critical Care Medicine
Siwei Wang, Fanchen Meng, Ming Li, Hua Bao, Xin Chen, Meng Zhu, Rui Liu, Xiuxiu Xu, Shanshan Yang, Xue Wu, Yang Shao, Lin Xu, Rong Yin
Summary: In this study, an accurate and affordable approach for early-stage lung cancer detection was established by integrating cfDNA fragmentomics and machine learning models. The stacked ensemble model achieved superior sensitivity for detecting early-stage lung cancer, which could promote early diagnosis and benefit more patients.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Review
Biology
Alberto Verlicchi, Matteo Canale, Elisa Chiadini, Paola Cravero, Milena Urbini, Kalliopi Andrikou, Luigi Pasini, Michele Flospergher, Marco Angelo Burgio, Lucio Crino, Paola Ulivi, Angelo Delmonte
Summary: This paper reviews the latest advances in using ctDNA to identify minimal residual disease (MRD) and predict prognosis in lung cancer patients after curative treatment. It focuses on the potential of ctDNA in clinical trials, the technical challenges faced, and the biological parameters influencing MRD detection.
Article
Oncology
Cloud P. Paweletz, Grace A. Heavey, Yanan Kuang, Emily Durlacher, Thian Kheoh, Richard C. Chao, Alexander I. Spira, Konstantinos Leventakos, Melissa L. Johnson, Sai-Hong Ignatius Ou, Gregory J. Riely, Kenna Anderes, Wenjing Yang, James G. Christensen, Pasi A. Janne
Summary: This study reports on early ctDNA changes of KRAS G12C in lung cancer patients and suggests that ctDNA changes can be used as a potential measure for early prediction of clinical response.
CLINICAL CANCER RESEARCH
(2023)
Article
Oncology
Ke Ye, Qinqiao Fan, Mingming Yuan, Dong Wang, Liang Xiao, Guo Long, Rongrong Chen, Tongdi Fang, Zengbo Li, Ledu Zhou
Summary: This study investigated the application of ctDNA sequencing in the postoperative period of hepatocellular carcinoma. The results showed that postoperative ctDNA was an independent prognostic predictor of disease-free survival and overall survival in liver cancer patients, and combining it with AFP improved prediction performance. Postoperative ctDNA sequencing has great prognostic value in patients with liver cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Marco Silvestri, Carolina Reduzzi, Giancarlo Feliciello, Marta Vismara, Thomas Schamberger, Caecilia Koestler, Rosita Motta, Stefano Calza, Cristina Ferraris, Andrea Vingiani, Giancarlo Pruneri, Maria Grazia Daidone, Christoph A. Klein, Bernhard Polzer, Vera Cappelletti
Summary: CTMs represent cancer cells detached from solid tumors and can provide insights into metastatic mechanisms. Analyzing CNAs in CTMs, which often contain unknown fractions of leukocytes, presents challenges. By titrating known numbers of leukocytes into cancer cells, we generated tumor fractions ranging from 0-100%. Through low-pass sequencing and the use of ichorCNA algorithm, we accurately predicted the tumor fraction of CTMs isolated from early-stage breast cancer patients. These CTMs were found to contain cancer cells with a range of 8-48%, with notable differences in CNAs compared to matched primary tumors, suggesting potential implications for metastasis-related genes that should be further explored.
Review
Cell Biology
Katerina Vlataki, Sevastiani Antonouli, Christina Kalyvioti, Evangeli Lampri, Sevasti Kamina, Davide Mauri, Haralampos V. Harissis, Angeliki Magklara
Summary: Liquid biopsies involve analyzing tumor-derived biological material in body fluids, primarily blood, to provide valuable clinical information for cancer management. They are non-invasive and can overcome limitations of tissue biopsy, aiding in making therapeutic decisions. Recent studies have shown the potential clinical utility of detecting circulating tumor DNA (ctDNA) in improving patient prognosis and monitoring therapy response, particularly in metastatic cancers. Technological advancements now allow accurate identification of ctDNA in early-stage cancers despite low levels in the bloodstream.
Article
Oncology
Ning Jiang, Jie Zhou, Wenhao Zhang, Peichao Li, Yu Liu, Hubo Shi, Chengke Zhang, Yunshan Wang, Chengjun Zhou, Chuanliang Peng, Weiquan Zhang, Yingtao Hao, Qifeng Sun, Yuliang Li, Xiaogang Zhao
Summary: The study found that the RNF213 gene mutated more frequently in lung cancer compared to benign pulmonary nodules both in peripheral blood and tumor tissue. Detection of RNF213 gene mutation in peripheral blood may be a highly specific method for assisting in the early diagnosis of lung cancer in pulmonary nodules.
Article
Oncology
Shuo Hu, Jinsheng Tao, Minhua Peng, Zhujia Ye, Zhiwei Chen, Haisheng Chen, Haifeng Yu, Bo Wang, Jian-Bing Fan, Bin Ni
Summary: This study identified 7 differentially methylated genes associated with lung cancer and developed a diagnostic model based on these genes to distinguish lung cancer from benign diseases. The model showed high accuracy and sensitivity in both tissue and plasma samples, suggesting its potential as a noninvasive method for early detection of lung cancer.
BIOMARKER RESEARCH
(2023)
Article
Oncology
Silvia Waldeck, Jan Mitschke, Sebastian Wiesemann, Michael Rassner, Geoffroy Andrieux, Max Deuter, Jurik Mutter, Anne-Marie Luechtenborg, Daniel Kottmann, Laurin Titze, Christoph Zeisel, Martina Jolic, Ulrike Philipp, Silke Lassmann, Peter Bronsert, Christine Greil, Justyna Rawluk, Heiko Becker, Lisa Isbell, Alexandra Mueller, Soroush Doostkam, Bernward Passlick, Melanie Boerries, Justus Duyster, Julius Wehrle, Florian Scherer, Nikolas von Bubnoff
Summary: The study revealed that intraoperative plasma sampling in early-stage and locally advanced NSCLC patients results in high ctDNA detection rates, and early postoperative ctDNA positivity serves as a prognostic indicator of disease relapse.
MOLECULAR ONCOLOGY
(2022)
Article
Oncology
Abhilasha Sinha, Yong Zou, Ayushi S. Patel, Seungyeul Yoo, Feng Jiang, Takashi Sato, Ranran Kong, Hideo Watanabe, Jun Zhu, Pierre P. Massion, Alain C. Borczuk, Charles A. Powell
Summary: This study found that MDM2 amplification and overexpression are associated with invasiveness and poor survival in lung adenocarcinoma. MDM2-targeted therapy effectively suppresses cell proliferation, migration, and invasion in cells with MDM2 amplification/overexpression, but not in cells without MDM2 amplification. Additionally, RNA sequencing identified different signaling pathways affected by MDM2-targeted therapy based on the p53 status of lung adenocarcinoma cells.
Article
Oncology
Chi-Cheng Huang, Yi-Fang Tsai, Chun-Yu Liu, Pei-Ju Lien, Yen-Shu Lin, Ta-Chung Chao, Chin-Jung Feng, Yen-Jen Chen, Jiun- Lai, Han-Fang Cheng, Bo-Fang Chen, Chih-Yi Hsu, Jen-Hwey Chiu, Ling-Ming Tseng
Summary: The VGH-TAYLOR study focused on early-stage breast cancer patients and performed targeted sequencing on both tumor tissue and plasma samples. The study found that only 25% of breast cancers showed concordance between tumor and liquid biopsy. The detection rate of liquid biopsy was 39% for all-stage breast cancers. The most frequently mutated genes were TP53 and KRAS, while mutations in PIK3CA, AKT1, and ERBB2 were potential biomarkers for FDA-approved therapeutics. Tumor tissue was shown to be a more reliable source for actionable mutation detection in early breast cancers.
Article
Cardiac & Cardiovascular Systems
Shao Dong Wang, Gan Wei Liu, Xiao Li, Xi Zhao Sui, Fan Yang, Jun Wang
Summary: The clinical relevance of highest mediastinal lymph node (HMLL) metastasis in patients with stage pT1-4N2M0 NSCLC is still controversial. Our study found that HMLL metastasis does not exhibit worse survival in these patients, and further examination is needed.
ANNALS OF THORACIC SURGERY
(2021)
Review
Cardiac & Cardiovascular Systems
Haifeng Shen, Xin Wang, Yuntao Nie, Kai Zhang, Zihan Wei, Fan Yang, Jun Wang, Kezhong Chen
Summary: For patients with locally advanced non-small-cell lung cancers, there was no significant difference in long-term oncological efficacy between minimally invasive surgery (MIS) and thoracotomy approaches. However, MIS was associated with significant advantages in perioperative outcomes, including a reduction in postoperative complications, intraoperative blood loss, chest tube drainage, and length of hospital stay.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2021)
Article
Oncology
Kezhong Chen, Yuntao Nie, Samina Park, Kai Zhang, Yangming Zhang, Yuan Liu, Bengang Hui, Lixin Zhou, Xun Wang, Qingyi Qi, Hao Li, Guannan Kang, Yuqing Huang, Yingtai Chen, Jiabao Liu, Jian Cui, Mingru Li, In Kyu Park, Chang Hyun Kang, Haifeng Shen, Yingshun Yang, Tian Guan, Yaxiao Zhang, Fan Yang, Young Tae Kim, Jun Wang
Summary: The study developed and validated a machine learning-based model for estimating the malignant probability of multiple pulmonary nodules, demonstrating excellent discrimination and calibration in the development cohort. External validation and prospective comparison showed higher AUC values and increased sensitivity and specificity. The model outperformed traditional models, clinicians, and a computer-aided diagnosis system, presenting solid performance as a convenient reference for decision-making.
CLINICAL CANCER RESEARCH
(2021)
Letter
Biochemistry & Molecular Biology
Kezhong Chen, Jianlong Sun, Heng Zhao, Ruijingfang Jiang, Jianchao Zheng, Zhilong Li, Jiaxi Peng, Haifeng Shen, Kai Zhang, Jin Zhao, Shida Zhu, Yuying Wang, Fan Yang, Jun Wang
Article
Oncology
Yi Ma, Mantang Qiu, Haifa Guo, Haiming Chen, Jiawei Li, Xiao Li, Fan Yang
Summary: COL6A6 is downregulated in LUAD, but high expression is associated with better prognosis and increased immune cell infiltration, indicating its potential role in immune modulation in LUAD.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Jun Ni, Miao Huang, Li Zhang, Nan Wu, Chun-Xue Bai, Liang-An Chen, Jun Liang, Qian Liu, Jie Wang, Yi-Long Wu, Feng-Chun Zhang, Shu-Yang Zhang, Chun Chen, Jun Chen, Wen-Tao Fang, Shu-Geng Gao, Jian Hu, Tao Jiang, Shan-Qing Li, He-Cheng Li, Yong-De Liao, Yang Liu, De-Ruo Liu, Hong-Xu Liu, Jian-Yang Liu, Lun-Xu Liu, Meng-Zhao Wang, Chang-Li Wang, Fan Yang, Yue Yang, Lan-Jun Zhang, Xiu-Yi Zhi, Wen-Zhao Zhong, Yu-Zhou Guan, Xiao-Xiao Guo, Chun-Xia He, Shao-Lei Li, Yue Li, Nai-Xin Liang, Fang-Liang Lu, Chao Lv, Wei Lv, Xiao-Yan Si, Feng-Wei Tan, Han-Ping Wang, Jiang-Shan Wang, Shi Yan, Hua-Xia Yang, Hui-Juan Zhu, Jun-Ling Zhuang, Ming-Lei Zhuo
Summary: Neoadjuvant immunotherapy is widely used in NSCLC patients and has shown improvements in major pathological remission rate, but carries risks of serious adverse reactions.
Article
Oncology
Yi Ma, Mantang Qiu, Shaodong Wang, Shushi Meng, Fan Yang, Guanchao Jiang
Summary: This study reveals that the bacterial microbiome composition of LUAD presenting as SSN is distinct from that of SN, showing higher richness and diversity in SSN. Microbiome signatures demonstrate good performance for predicting LUAD or SSN with high accuracy.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Oncology
Xianping Liu, Kunkun Sun, Fan Yang, Xizhao Sui, Guanchao Jiang, Jun Wang, Xiao Li
Summary: The study aimed to accurately describe the patterns, timing, and predictors of disease recurrence after curative resection for different types of early-stage lung adenocarcinoma (LUAD). High-grade adenocarcinoma (HGADC) was found to be an independent predictor for recurrence, with different recurrence patterns and risks compared to low-grade adenocarcinoma (LGADC), which showed a smoother curve after surgery.
Article
Cell Biology
Guangxi Wang, Mantang Qiu, Xudong Xing, Juntuo Zhou, Hantao Yao, Mingru Li, Rong Yin, Yan Hou, Yang Li, Shuli Pan, Yuqing Huang, Fan Yang, Fan Bai, Honggang Nie, Shuangshuang Di, Limei Guo, Zhu Meng, Jun Wang, Yuxin Yin
Summary: Lung cancer, the leading cause of cancer-related deaths, can be improved by early detection. A study found dysregulated lipid metabolism in different cell types of early-stage lung cancers. By identifying nine important lipids, a targeted assay was developed with high specificity in detecting early-stage lung cancer. This method demonstrated high sensitivity and specificity in screening and clinical cohorts. Overall, this method shows promise in early lung cancer detection and cancer prevention for high-risk populations.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Yanmeng Li, Xiao Li, Haiming Chen, Kunkun Sun, Hao Li, Ying Zhou, Jun Wang, Fan Bai, Fan Yang
Summary: This study explored the characteristics of the tumor microenvironment and transcriptional features of malignant cells in early-stage lung adenocarcinoma with radiological part-solid nodules.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Hao Li, Zewen Sun, Rongxin Xiao, Qingyi Qi, Xiao Li, Haiyan Huang, Xuan Wang, Jian Zhou, Zhenfan Wang, Ke Liu, Ping Yin, Fan Yang, Jun Wang
Summary: This study aimed to uncover the genomic characteristics and evolution of early-stage subsolid lung adenocarcinomas (SSN-LUADs) presenting as pure ground-glass nodules (pGGNs), heterogeneous ground-glass nodules (HGGNs), and part-solid nodules (PSNs). The results showed that HGGNs had significantly lower tumor mutation count, genomic alteration count, and intra-tumor heterogeneity compared to PSNs. There were statistically significant upward trends in alterations involving driver mutations and oncogenic pathways from pGGNs to HGGNs to PSNs. EGFR mutation was identified as a key early event in the progression of SSN-LUADs.
BRITISH JOURNAL OF CANCER
(2022)
Article
Cardiac & Cardiovascular Systems
Hao Xu, Tingxuan Han, Haifeng Wang, Shanggui Liu, Guanghao Hou, Lina Sun, Guanchao Jiang, Fan Yang, Jun Wang, Ke Deng, Jian Zhou
Summary: The objective of this study was to develop high throughput computer vision (CV) algorithms for detecting blood stains in thoracoscopic surgery and determining their association with postoperative outcomes. Blood pixels in surgical videos were identified using CV algorithms trained with thousands of blood and non-blood pixels. The proportion of blood pixels (PBP) was computed to summarize blood stain information. Statistical regression analyses revealed potential associations between PBP and postoperative outcomes.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2022)
Article
Gastroenterology & Hepatology
Feng Yang, Jian Gao, Sida Cheng, Hao Li, Kunshan He, Jian Zhou, Kezhong Chen, Zhenfan Wang, Fan Yang, Zeyu Zhang, Jianfeng Li, Zuli Zhou, Chongwei Chi, Yun Li, Jun Wang
Summary: This study explored the feasibility and effectiveness of using near-infrared fluorescence imaging with indocyanine green to identify thoracic ducts and chyle leakage during video-assisted thoracoscopic esophagectomy. The results showed that this technique can provide highly sensitive and real-time assessment of thoracic ducts, as well as determine the source of chyle leakage, which may help reduce thoracic duct injury and guide selective ligation.
DISEASES OF THE ESOPHAGUS
(2023)
Review
Nutrition & Dietetics
Peiyu Wang, Shaodong Wang, Yi Ma, Haoran Li, Zheng Liu, Guihu Lin, Xiao Li, Fan Yang, Mantang Qiu
Summary: The meta-analysis of 26 studies identified a correlation between sarcopenic obesity and increased complications and reduced survival rates in patients with gastrointestinal surgical oncology, suggesting that interventions targeting sarcopenic obesity preoperatively may improve surgical outcomes for these patients.
FRONTIERS IN NUTRITION
(2022)
Article
Oncology
Kezhong Chen, Fan Yang, Haifeng Shen, Chenyang Wang, Xi Li, Olga Chervova, Shuailai Wu, Fujun Qiu, Di Peng, Xin Zhu, Shannon Chuai, Stephan Beck, Nnennaya Kanu, David Carbone, Zhihong Zhang, Jun Wang
Summary: We report a personalized tumor-informed technology, PROPHET, which uses deep sequencing to detect patient-specific variants for molecular residual disease (MRD) detection. PROPHET shows higher sensitivity and predictive ability, and can be used for MRD detection and prognostic analysis in early stage lung cancer patients.