期刊
SCIENTIFIC REPORTS
卷 6, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/srep27085
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资金
- Institut Pasteur (Paris)
- France-BioImaging infrastructure network - French National Research Agency [ANR-10-INSB-04]
- Region Ile-de-France (program DIM-Malinf)
- French Agence Nationale de la Recherche [ANR-09-JCJC-0045-01]
- European Research Council [ERC StG 242703, ERC CoG 615220]
- LabEx IBEID (Integrative Biology of Emerging Infectious Diseases) program
- Agence Nationale de la Recherche (ANR) [ANR-09-JCJC-0045] Funding Source: Agence Nationale de la Recherche (ANR)
Tunnelling nanotubes and cytonemes function as highways for the transport of organelles, cytosolic and membrane-bound molecules, and pathogens between cells. During viral infection in the model organism Drosophila melanogaster, a systemic RNAi antiviral response is established presumably through the transport of a silencing signal from one cell to another via an unknown mechanism. Because of their role in cell-cell communication, we investigated whether nanotube-like structures could be a mediator of the silencing signal. Here, we describe for the first time in the context of a viral infection the presence of nanotube-like structures in different Drosophila cell types. These tubules, made of actin and tubulin, were associated with components of the RNAi machinery, including Argonaute 2, double-stranded RNA, and CG4572. Moreover, they were more abundant during viral, but not bacterial, infection. Super resolution structured illumination microscopy showed that Argonaute 2 and tubulin reside inside the tubules. We propose that nanotube-like structures are one of the mechanisms by which Argonaute 2, as part of the antiviral RNAi machinery, is transported between infected and non-infected cells to trigger systemic antiviral immunity in Drosophila.
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