Article
Neurosciences
Shiying Li, Wenshuang Wu, Jing Zhang, Yu Chen, Yumeng Wu, Xinghui Wang
Summary: The study showed that miR-195-5p is dysregulated after peripheral nerve injury and plays a critical regulatory role in Schwann cell viability, proliferation, and migration. It also affects peripheral nerve regeneration by targeting the Crebl2 gene and modulating Schwann cell functions.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Neurosciences
Pingping Qiao, Wenshuang Wu, Yumeng Wu, Xinghui Wang
Summary: This study found that miR-328a-3p expression was upregulated after nerve damage. Elevated miR-328a-3p levels suppressed Schwann cell viability, proliferation, and migration.
NEUROSCIENCE LETTERS
(2022)
Article
Neurosciences
Jun Sun, Zhi Liao, Zhangyu Li, Hao Li, Zhimin Wu, Chuan Chen, Hui Wang
Summary: This study focused on investigating the roles and mechanisms of exosomes derived from Schwann cells under different conditions in regulating macrophagic sub-phenotypes and peripheral nerve injury repair. The study found that exosomes from normal oxygen condition Schwann cells promoted M2 macrophagic polarization and facilitated axon elongation, while exosomes from post-injury oxygen-glucose-deprivation-condition Schwann cells promoted M1 polarization and inhibited axon elongation. The down-regulation of miR-146a-5p was closely related to the shift of exosomes to pro-inflammatory phenotype. The study concluded that miR-146a-5p played an important role in regulating macrophagic phenotype and had implications for axonal regeneration and functional recovery.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Neurosciences
Min You, Haizhu Xing, Ming Yan, Jie Zhang, Jiayi Chen, Yang Chen, Xiaoli Liu, Jing Zhu
Summary: Paclitaxel-induced peripheral neuropathy (PIPN) is a neurological disorder caused by paclitaxel (PTX) treatment in cancer patients. Schwann cell-derived exosomes (SC-EXOs) have been found to effectively improve PIPN both in vitro and in vivo. SC-EXOs alleviate PTX-induced mechanical nociceptive sensitization, ameliorate nerve fiber loss and DRG injury, and protect damaged neurons by upregulating miR-21 to repress the PTEN signaling pathway. These findings provide a novel strategy for the treatment of PIPN.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Veterinary Sciences
Tianmei Qian, Pingping Qiao, Yingnan Lu, Hongkui Wang
Summary: The study revealed a potential role of SS18L1 in peripheral nerve injury, showing its influence on nerve recovery process by regulating Schwann cells proliferation, migration, and differentiation.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Anton Borger, Sarah Stadlmayr, Maximilian Haertinger, Lorenz Semmler, Paul Supper, Flavia Millesi, Christine Radtke
Summary: miRNAs play a crucial role in peripheral nerve injuries by regulating specific pathways and proteins, which is essential for understanding the molecular mechanisms of nerve regeneration and providing targets for precision medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Jue Ling, Chang He, Shuxuan Zhang, Yahong Zhao, Meifeng Zhu, Xiaoxuan Tang, Qiaoyuan Li, Liming Xu, Yumin Yang
Summary: This article provides an overview of evaluation methods used to study scaffold-based therapies for peripheral nerve injury (PNI) in experimental animal models, with particular focus on Schwann cell functions and axonal growth within the regenerated nerve.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sailing Chen, Qianqian Chen, Xiaojiao Zhang, Yinying Shen, Xinyu Shi, Xiu Dai, Sheng Yi
Summary: Peripheral nerves have limited ability to regenerate after injury. The growth factor amphiregulin (AREG) is upregulated in Schwann cells of injured sciatic nerves and stimulates their proliferation and migration. Schwann cell-secreted AREG promotes neurite outgrowth and axon elongation. Administering AREG to injured nerves facilitates Schwann cell proliferation, cord formation, and axon regrowth. Overall, our study identifies AREG as an important neurotrophic factor and offers a promising therapeutic approach for peripheral nerve injuries.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Cell Biology
Silvan Klein, Andreas Siegmund, Andreas Eigenberger, Valerie Hartmann, Felix Langewost, Nicolas Hammer, Alexandra Anker, Konstantin Klein, Christian Morsczeck, Lukas Prantl, Oliver Felthaus
Summary: The study investigated three differentiation protocols for differentiating rat ASCs into specialized SC phenotypes, comparing their expressions of neurotrophins and myelin markers. The results showed diverse expression profiles of differentiated ASCs, with different protocols inducing varying levels of expression of neurotrophins and myelin markers.
Article
Cell Biology
Yazhen Xu, Bingyu Guo, Xiaoyan Liu, Kai Tao
Summary: miR-34a inhibits melanoma growth by targeting ZEB1, suggesting a potential novel target for melanoma treatment.
Article
Biochemistry & Molecular Biology
Yaxian Wang, Fuchao Zhang, Yunsong Zhang, Qi Shan, Wei Liu, Fengyuan Zhang, Feiyu Zhang, Sheng Yi
Summary: Research findings indicate that Btc plays essential roles in regulating Schwann cell migration and axon elongation, suggesting the potential application of Btc as a regenerative strategy for treating peripheral nerve injury.
MOLECULAR MEDICINE
(2021)
Article
Biology
Tian-Kun Hui, Xin-Sheng Lai, Xia Dong, Hongyang Jing, Ziyang Liu, Erkang Fei, Wen-Bing Chen, Shunqi Wang, Dongyan Ren, Suqi Zou, Hai-Tao Wu, Bing-Xing Pan
Summary: Schwann cells play a crucial role in peripheral nerve regeneration, while the conditional knockout of Lrp4 in SCs contributes to enhanced nerve regeneration by promoting SC proliferation and myelin debris clearance. These results suggest a novel role for Lrp4 in peripheral nerve recovery and provide a potential therapeutic target.
Article
Pharmacology & Pharmacy
Ane Escobar, Mariana R. Carvalho, F. Raquel Maia, Rui L. Reis, Tiago H. Silva, Joaquim M. Oliveira
Summary: This study demonstrates the effectiveness of using CMCht/PAMAM NPs as a GDNF release system for peripheral nerve regeneration, providing stable GDNF release under physiological conditions and improving cell viability as well as enhancing the uptake rate of neural cells.
Article
Biochemistry & Molecular Biology
Yoo Lim Chun, Ki-Hoon Park, Badvel Pallavi, Won-Joon Eom, Chan Park, Youngbuhm Huh, Yeonjoo Lee, Jimin Lee, Sang Hoon Kim, Seung Geun Yeo, Hyung-Joo Chung, Byeong-Seon Kim, Na Young Jeong, Junyang Jung
Summary: The study found that the new cinnamaldehyde derivative 3f has the strongest effect in inhibiting peripheral nerve degeneration, suggesting its potential for the development of novel synthetic drugs. Molecular docking studies and bioinformatics analysis revealed that 3f mainly interacts with the target protein through hydrophobic interactions and affects oxidative stress in Schwann cells.
Article
Clinical Neurology
Qin Zhang, Chengkun Guo, Lijuan Liu, Yang Li
Summary: This study investigated the role of miR-148b-3p in Schwann cells after peripheral nerve injury. The expression level of miR-148b-3p and USP6 was assessed in a rat model of sciatic nerve transection, and cell migration and proliferation were examined in primary Schwann cells. The results showed that overexpression of miR-148b-3p inhibited Schwann cell proliferation and migration, while suppression of miR-148b-3p had the opposite effect. USP6 mRNA was identified as a target of miR-148b-3p, and its silencing suppressed Schwann cell migration and proliferation.
NEUROLOGICAL RESEARCH
(2023)
Article
Cell Biology
Ruirui Zhang, Qi Chen, Li Huang, Yunsong Zhang, Xinghui Wang, Sheng Yi
Summary: The study analyzed single-cell transcriptional profiling of Schwann cells in neonatal rat sciatic nerves, revealing cellular heterogeneity and dynamic changes. It also identified differentiation trajectory from certain Schwann cell subtypes to others, with functional interpretation showing genetic signatures of DNA replication and acquisition of mesenchymal traits in subtype 3 Schwann cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Neurosciences
Yinying Shen, Zhangchun Cheng, Sailing Chen, Yunsong Zhang, Qi Chen, Sheng Yi
Summary: miR-29a-3p is up-regulated in injured sciatic nerves and inhibits Schwann cell proliferation and migration by negatively regulating PMP22. Injection of miR-29a-3p agomir hinders Schwann cell activities, delays axon elongation and myelination, and retards the functional recovery of injured nerves. This highlights the important role of miR-29a-3p/PMP22 in regulating Schwann cell phenotype following sciatic nerve injury and peripheral nerve regeneration.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Cell Biology
Yin-Ying Shen, Rui-Rui Zhang, Qian-Yan Liu, Shi-Ying Li, Sheng Yi
Summary: Cellular senescence and proliferation are essential for wound healing and tissue remodeling. This study analyzed gene expression patterns in rat sciatic nerve stumps and dorsal root ganglia after injury to reveal the genetic changes associated with cellular senescence and proliferation. The study found that cellular senescence and proliferation were less vigorous in the dorsal root ganglia compared to the sciatic nerve stumps.
NEURAL REGENERATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yunsong Zhang, Miao Yang, Yinying Shen, Sheng Yi, Xinghui Wang
Summary: This study found that MOGS is upregulated in nerve segments after peripheral nerve injury, and bioinformatic analysis indicated its involvement in cellular metabolism and negative regulation on Schwann cell plasticity and metabolic coupling with axons.
JOURNAL OF MOLECULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Yunsong Zhang, Qian Zhao, Qianqian Chen, Lingchi Xu, Sheng Yi
Summary: Transcription factors play important roles in nerve regeneration after peripheral nerve injury, including regulating axon elongation and Schwann cell phenotype modulation. Understanding these transcription factors can help promote functional recovery of injured peripheral nerves.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Cell Biology
Qian-Qian Chen, Qian-Yan Liu, Pan Wang, Tian-Mei Qian, Xing-Hui Wang, Sheng Yi, Shi-Ying Li
Summary: Neurotrophic factors, especially nerve growth factor, can enhance neuronal regeneration, but their in vivo applications are limited. This study investigated the potential of let-7 as a regulator in nerve repair, as it targets and regulates nerve growth factor. Anti-let-7a was identified as the most suitable let-7 family molecule, and its controlled delivery was achieved using a chitosan-hydrogel scaffold, promoting nerve regeneration and functional recovery in a rat model of sciatic nerve transection.
NEURAL REGENERATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Qianqian Chen, Yang Miao, Zhipeng Xu, Ruirui Zhang, Sheng Yi
Summary: Schwann cells play a crucial role in nerve regeneration and are commonly used in tissue engineering. Identifying Schwann cells with enhanced migration ability is important for improving recovery effects. This study compared Schwann cells from different anatomical locations and found that dorsal root ganglia (DRG) Schwann cells have faster migration speed than sciatic nerve (SN) Schwann cells.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Neurosciences
Lili Zhao, Weixiao Huang, Sheng Yi
Summary: Single-cell RNA sequencing enables the classification and transcriptional profiling of individual cells, leading to a better understanding of cellular diversity. In the peripheral nervous system (PNS), this technique has identified multiple cell types including neurons, glial cells, ependymal cells, immune cells, and vascular cells. Subtypes of neurons and glial cells have also been recognized in different physiological and pathological states. This article summarizes the heterogeneity of cells in the PNS and describes cellular variability during development and regeneration, which provides insights into the complex cellular architecture of the PNS and serves as a basis for future genetic manipulation.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Sailing Chen, Qianqian Chen, Xiaojiao Zhang, Yinying Shen, Xinyu Shi, Xiu Dai, Sheng Yi
Summary: Peripheral nerves have limited ability to regenerate after injury. The growth factor amphiregulin (AREG) is upregulated in Schwann cells of injured sciatic nerves and stimulates their proliferation and migration. Schwann cell-secreted AREG promotes neurite outgrowth and axon elongation. Administering AREG to injured nerves facilitates Schwann cell proliferation, cord formation, and axon regrowth. Overall, our study identifies AREG as an important neurotrophic factor and offers a promising therapeutic approach for peripheral nerve injuries.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wanling Chen, Ting Gu, Qianqian Chen, Chuxiang Qu, Chunye Zhang, Yuhua Hu, Ronghui Xia, Ying Zhang, Min Wang, Xinyi Huang, Jiang Li, Chaoji Shi, Zhen Tian
Summary: This study investigated the role of extracellular matrix (ECM) modifications in the tumorigenesis of salivary carcinoma ex pleomorphic adenoma (CXPA). The researchers found that excessive deposition of hyaluronic acid and alterations in the ECM play a critical role in the development of CXPA. These findings provide new insights into the mechanism of CXPA tumorigenesis.
CELL AND BIOSCIENCE
(2023)