Article
Biochemical Research Methods
Shaobo Yang, Hong Yang, Yanlei Huang, Gong Chen, Chun Shen, Shan Zheng
Summary: Hirschsprung's disease (HSCR) is a congenital digestive tract malformation characterized by the absence of intramural ganglion cells in the myenteric and submucosal plexuses along variable lengths of the gastrointestinal tract. This study used metabolomic profiling to analyze HSCR serum samples and identified 21 biomarkers related to HSCR. It revealed that tryptophan metabolism was a crucial disordered pathway in HSCR. This is the first serum metabolomics study focusing on HSCR and provides a new perspective for explaining its mechanism.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Immunology
Meilin Ding, Zha Zhen, Mei Ju, Suolang Quzong, Xuesi Zeng, Xiaoxia Guo, Rui Li, Mingming Xu, Jingjing Xu, Hongyang Li, Wei Zhang
Summary: Our study found significant differences in the metabolomic profiles between vitiligo patients and healthy subjects in both plateau and plain areas, with significantly higher levels of S1P in the serum of vitiligo patients and healthy subjects in the plateau area. These findings suggest that alterations in S1P metabolism may be involved in the pathogenesis of vitiligo.
CLINICAL IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Tiago A. H. Fonseca, Cristiana P. Von Rekowski, Ruiben Araujo, M. Conceicao Oliveira, Goncalo C. Justino, Luis Bento, Ceciïlia R. C. Calado
Summary: Biofluid metabolomics is a powerful tool for studying disease mechanisms and biomarkers. This study evaluated the impact of two extraction protocols on serum metabolome analysis. The results showed that different protocols can affect the number and type of features identified, as well as the reproducibility of the analysis.
Article
Biochemistry & Molecular Biology
Md Motiur Rahaman, Rebecca S. Zwart, Thusitha W. T. Rupasinghe, Helen L. Hayden, John P. Thompson
Summary: Metabolic profiling of resistant and susceptible wheat genotypes revealed that certain metabolites constitutively expressed in resistant roots could reduce nematode reproduction, while susceptible wheat may induce metabolites that attract nematodes. The study suggests that specific metabolites may affect the strength of root cell walls and nematode penetration, highlighting differences between resistant and susceptible wheat in their response to nematode infection.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Nutrition & Dietetics
Dadi Xie, Yanbo Zhang, Yujin Guo, Xianzhong Xue, Shiyuan Zhao, Chunmei Geng, Yuanyuan Li, Rui Yang, Yizhang Gan, Hanbing Li, Zhongfa Ren, Pei Jiang
Summary: The objective of this research was to observe and evaluate the impact of dietary components on metabolomic profiles in primary tissues of mice. The levels of metabolites in mice were studied and analyzed using gas chromatography-mass spectrometry. The results showed significant differences in metabolites related to energy, amino acids, and lipid metabolism.
FRONTIERS IN NUTRITION
(2023)
Article
Chemistry, Analytical
Dezhi Shan, Peng Qi, Daming Wang
Summary: Cerebrovascular stenosis (CVS) is the main cause of ischemic stroke and early screenings for CVS are important. This study aimed to elucidate the correlation between CVS and metabolomics to aid in early CVS screening. Differential metabolites were identified in patients with CVS, providing insights into the pathogenesis and early diagnosis of CVS.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Juan J. Aristizabal-Henao, Dominick J. Lemas, Fmily K. Griffin, Kaylie Anne Costa, Camden Camacho, John A. Bowden
Summary: The number of metabolomics studies has increased significantly in recent years, with advancements in analyte separation techniques and databases playing a major role in this growth. Establishing baseline metabolomics data using complementary data acquisition strategies can help address challenges in comparing data across studies. This study provides valuable nontargeted metabolomics data for various human reference materials, highlighting significant differences between sample types and supporting the use of nontargeted strategies in global metabolomics.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Isabel Meister, Pei Zhang, Anirban Sinha, C. Magnus Skold, Asa M. Wheelock, Takashi Izumi, Romanas Chaleckis, Craig E. Wheelock
Summary: This study developed a method for measuring specific gravity in urine using a refractive index detector (RID) in a 96-well-plate format, providing a new solution for metabolomic research on this noninvasive biofluid. By developing an automated LC-MS workflow and utilizing multiple technical internal standards to monitor data quality, over 540 urinary metabolites were successfully identified.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Shuo Wei, Yi-sheng Chen, Yi Shi
Summary: This study identified potential biomarkers for the diagnosis of pulmonary aspergillosis (PA) by analyzing the metabolic profile of exhaled breath condensate (EBC) samples. The identified compounds showed good classification between PA patients and controls, and can serve as noninvasive markers for the diagnosis of PA.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Agronomy
Yongquan Li, Bipei Zhang, Runsheng Huang, Min Wen, Leying Huang, Yiting Su, Yanjun Sun, Ning Wang, Wei Guo
Summary: The drought stress responses of plants are complex regulatory mechanisms that can be studied through metabolomics. In this study, the effect of drought stress on a drought-tolerant cultivar of Belosynapsis ciliata was investigated using metabolomic analysis. Different metabolites and pathways related to drought tolerance were identified.
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Cardiac & Cardiovascular Systems
Siddabasave Gowda B. Gowda, Divyavani Gowda, Fengjue Hou, Hitoshi Chiba, Vibhu Parcha, Pankaj Arora, Ganesh V. Halade, Shu-Ping Hui
Summary: This study conducted a comprehensive lipidomic analysis on heart disease patients and healthy individuals, revealing significant differences in lipid profiles between ischemic patients and healthy subjects. Different lipid metabolites were found in the transition from acute to chronic heart failure, with marked increases in PE levels in chronic MI patients, possibly indicating cardiac remodeling.
Article
Physiology
Eden A. Houske, Matthew G. Glimm, Annika R. Bergstrom, Sally K. Slipher, Hope D. Welhaven, Mark C. Greenwood, Greta M. Linse, Ronald K. June, Alan S. L. Yu, Darren P. Wallace, Alyssa K. Hahn
Summary: This study analyzed the urine metabolites of early-stage ADPKD patients and identified metabolic pathway alterations and candidate diagnostic and prognostic biomarkers. The results provide potential therapeutic targets and biomarkers for early diagnosis and tracking disease progression of ADPKD.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Biochemical Research Methods
Kaushik Kumar Dey, Jay M. Yarbro, Danting Liu, Xian Han, Zhen Wang, Yun Jiao, Zhiping Wu, Shu Yang, Donggeun Lee, Abhijit Dasgupta, Zuo-Fei Yuan, Xusheng Wang, Liqin Zhu, Junmin Peng
Summary: Alzheimer's disease (AD) is the most common form of dementia, with a greater impact on women in terms of prevalence and progression. In this study, researchers conducted a comprehensive analysis of the serum proteome in a common AD mouse model to identify potential biomarkers associated with AD pathology and gender. Using a new proteome profiling method, they successfully identified 3972 unique proteins and detected 60 sex-specific proteins.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Analytical
Yuting Wang, Yisheng Wang, Chen Chen, Fang Ren, Rui Cao, Yuefei Wang, Pin Han, Xiaoyan Zhang, Congjian Xu, Xinyu Liu, Guowang Xu
Summary: The study identified lipid disturbances in the serum of patients with epithelial ovarian cancer (EOC) and established 4 lipids as potential markers for aiding EOC diagnosis. These markers showed high accuracy in distinguishing EOC and early EOC from non-cancer, with good specificity and sensitivity. The research not only revealed the characteristics of lipid metabolism in EOC, but also provided a potential marker pattern for aiding EOC diagnosis.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Immunology
Jin-Lei Wang, Qin-Li Liang, Ting-Ting Li, Jun-Jun He, Meng-Jie Bai, Xue-Zhen Cao, Hany M. Elsheikha, Xing-Quan Zhu
JOURNAL OF IMMUNOLOGY
(2020)
Article
Microbiology
Jun-Jun He, Jun Ma, Jin-Lei Wang, Fu-Kai Zhang, Jie-Xi Li, Bin-Tao Zhai, Hany M. Elsheikha, Xing-Quan Zhu
Article
Microbiology
Hany M. Elsheikha, Mamdowh Alkurashi, Suzy Palfreman, Marcos Castellanos, Kenny Kong, Evita Ning, Nashwa A. Elsaied, Kalotina Geraki, William MacNaughtan
Review
Microbiology
Hany M. Elsheikha, Christina M. Marra, Xing-Quan Zhu
CLINICAL MICROBIOLOGY REVIEWS
(2020)
Article
Infectious Diseases
Wei Shi, Jun-Jun He, Xue-Fang Mei, Ke-Jing Lu, Zi-Xuan Zeng, Yao-Yao Zhang, Zhao-An Sheng, Hany M. Elsheikha, Wei-Yi Huang, Xing-Quan Zhu
PLOS NEGLECTED TROPICAL DISEASES
(2020)
Article
Microbiology
Rui-Si Hu, Fu-Kai Zhang, Hany M. Elsheikha, Qiao-Ni Ma, Muhammad Ehsan, Quan Zhao, Xing-Quan Zhu
Article
Parasitology
Ze-Xiang Wang, Xi Chen, Liang Han, Huai-Dong Liu, Jun-Hui Guo, Yu Zhao, Xiao-Lin Sun
Summary: Taenia pisiformis is a parasite causing cysticercosis pisiformis, and the 18-kDa oncosphere antigen is essential for diagnosis and monoclonal antibody production. MAb 7E8, targeting TPO18, showed the highest titer and specific reaction, providing a potential diagnostic tool for Taenia pisiformis infections. This research contributes to understanding TPO18 functions and developing diagnostic methods.
EXPERIMENTAL PARASITOLOGY
(2021)
Article
Immunology
Ze-Xiang Wang, Rui-Si Hu, Xing-Quan Zhu, Xiao-Lin Sun, Hany M. Elsheikha
Summary: This study investigated the phosphoproteomic landscape of sporulated oocysts between virulent and avirulent Toxoplasma gondii strains, revealing significant differences in phosphopeptide profiles. Various motifs were identified in the differentially expressed phosphopeptides, and several kinases were associated with overrepresented phosphopeptides. Overall, the findings provide new insights into the mechanisms underlying the virulence of T. gondii.
MICROBIAL PATHOGENESIS
(2021)
Review
Infectious Diseases
Man-Yao Li, Xiao-Nan Gao, Jun-Yang Ma, Hany M. Elsheikha, Wei Cong
Summary: The global prevalence of T. gondii infection in wild marine mammals is estimated to be approximately 22.44%. The prevalence is higher in adult animals compared to younger age groups. North America has the highest prevalence among continents, and Spain has the highest prevalence among countries. Climatic variables, including a mean annual temperature >20 degrees C and an annual precipitation >800 mm, are associated with higher prevalence.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Cell Biology
Hany M. Elsheikha, Alaa T. Al-Sandaqchi, Mohammad S. R. Harun, Francesca Winterton, Ali Altharawi, Nashwa A. Elsaied, Carl W. Stevenson, William MacNaughtan, John G. M. Mina, Paul W. Denny, Gianfelice Cinque, Ka Lung Andrew Chan
Summary: This study used FTIR microspectroscopy and synchrotron-based FTIR microspectroscopy to analyze the chemical changes associated with T. gondii infection of hBMECs. The results showed that both methods were able to reveal the chemical alterations in the infected cells. Furthermore, RNA sequencing analysis revealed significant changes in host cell genes and pathways in response to T. gondii infection.
Article
Pharmacology & Pharmacy
Mohamed A. Helal, Ahmed M. Abdel-Gawad, Omnia M. Kandil, Marwa M. E. Khalifa, Alison A. Morrison, David J. Bartley, Gareth W. Cave, Hany M. Elsheikha
Summary: In this study, chitosan coated PLGA particles were used to encapsulate essential oil components, which showed potent anthelmintic activity against ovine gastrointestinal nematodes. The encapsulated particles improved the bioavailability and efficacy of the essential oil components.
Article
Parasitology
Lenu B. Fakae, Mohammad S. R. Harun, Darren Shu Jeng Ting, Harminder S. Dua, Gareth W. V. Cave, Xing-Quan Zhu, Carl W. Stevenson, Hany M. Elsheikha
Summary: We investigated the anti-acanthamoebic effect of green tea Camellia sinensis solvent extract and its chemical components against Acanthamoeba castellanii. The extract and two specific components, epigallocatechin-3-gallate and caffeine, were found to inhibit both the replication and encystation of the parasite. The extract did not show cytotoxicity to human corneal epithelial cells and Madin-Darby canine kidney cells. Further pre-clinical studies are needed to evaluate the efficacy and safety of the extract in vivo.
Article
Multidisciplinary Sciences
Jin-Lei Wang, Ting-Ting Li, Hany M. Elsheikha, Qin-Li Liang, Zhi-Wei Zhang, Meng Wang, L. David Sibley, Xing-Quan Zhu
Summary: Phenotypic switching between tachyzoite and bradyzoite is crucial for the pathogenicity and adaptability of Toxoplasma gondii. The protein phosphatase 2A (PP2A) was found to play a key regulatory role in starch metabolism and bradyzoite differentiation in T. gondii.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Xiao-Nan Zheng, Jin-Lei Wang, Hany M. M. Elsheikha, Meng Wang, Zhi-Wei Zhang, Li-Xiu Sun, Xin-Cheng Wang, Xing-Quan Zhu, Ting-Ting Li
Summary: The analysis of the subcellular localization and function of dense granule proteins is crucial for understanding the pathogenesis of Toxoplasma gondii infection. This study identified 15 novel GRAs and determined their localization and functions in different life cycle stages and strains of T. gondii. Deletion of TGME49_266410 and TGME49_315910 significantly affected parasite growth and virulence, demonstrating their importance in T. gondii pathogenicity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Wei Wang, Yi Yang, Huanping Guo, Ming-Han Li, Xiao-Qing Chen, Xin-Yu Wei, Yu Chen, Hany M. M. Elsheikha, Xiao-Xuan Zhang
Summary: Using liquid chromatography-tandem mass spectrometry, we identified RNA modifications in three strains of Toxoplasma gondii and compared the diversity and patterns of modifications between different strains. We found significant differences in the modified nucleotides in tRNA and sncRNA, which were correlated with the expression of virulence proteins. These findings provide new insights into the diversity of RNA modifications in T. gondii and their potential role in virulence.
MICROBIOLOGY SPECTRUM
(2023)
