Article
Cell & Tissue Engineering
Haoteng Yan, Jiali Zhu, Yanyan Ping, Min Yan, Gaoming Liao, Huating Yuan, Yao Zhou, Fengyu Xiang, Bo Pang, Jinyuan Xu, Lin Pang
Summary: In this study, single-cell transcriptome sequencing data of 6 GBM cohorts were used to identify primary GSCs and characterize their cell states. Two recurrent GSCs subpopulations, pGSCs and qGSCs, were identified based on their cell cycle activity. The processes of tumor development were traced and it was found that GSCs accumulated throughout the whole period, with pGSCs mainly contributing to the early stage and qGSCs enriched in the later stage. Furthermore, an 8-gene prognostic signature reflecting pGSCs activity was constructed and associated with poor clinical outcomes.
Review
Cell Biology
Amrutha Mohan, Reshma Raj Rajan, Gayathri Mohan, Padmaja Kollenchery Puthenveettil, Tessy Thomas Maliekal
Summary: Cancer stem cells (CSCs) exhibit functional heterogeneity and hierarchical organization, giving rise to tumor-initiating cells, drug-resistant cells, or metastasis-initiating cells. Various markers are used to identify CSCs, with a combination of markers used to demarcate the heterogeneous CSCs in solid tumors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Delphine Loussouarn, Lisa Oliver, Celine Salaud, Edouard Samarut, Raphael Bourgade, Christophe Beroud, Emilie Morenton, Dominique Heymann, Francois M. M. Vallette
Summary: This study evaluates the distribution of immune biomarkers in primary and recurrent GBM using immunohistochemistry and Nanostring Geomix Digital spatial profiling. The results show differential localization of HLA-DR and B7-H3 between primary and recurrent GBM, but no correlation between the immune landscape during the evolution of GBM. This study suggests B7-H3 and HLA-DR as potential targets in primary and recurrent GBM.
Article
Oncology
Jennifer Eymael, Martijn van den Broek, Laura Miesen, Valerie Villacorta Monge, Bartholomeus T. van den Berge, Fieke Mooren, Vicky Luna Velez, Jelmer Dijkstra, Meyke Hermsen, Peter Bandi, Michiel Vermeulen, Saskia de Wildt, Brigith Willemsen, Sandrine Florquin, Roy Wetzels, Eric Steenbergen, Rafael Kramann, Marcus Moeller, Michiel F. Schreuder, Jack Fm Wetzels, Johan van der Vlag, Jitske Jansen, Bart Smeets
Summary: Scattered tubular cells (STCs) in the proximal tubule increase in number after acute kidney injury. STCs are preferentially located within the inner bends of the tubule and their number increases with age. STCs represent a transient state of dedifferentiated proximal tubule epithelial cells (PTECs) showing metabolic shift towards glycolysis, facilitating cellular survival after kidney injury.
JOURNAL OF PATHOLOGY
(2023)
Article
Immunology
Sheridan L. Swan, Nalini Mehta, Ekaterina Ilich, Steven H. Shen, Daniel S. Wilkinson, Alexa R. Anderson, Tatiana Segura, Luis Sanchez-Perez, John H. Sampson, Ravi V. Bellamkonda
Summary: Chimeric antigen receptor (CAR) T cell therapy in glioblastoma faces challenges with CAR T cell abundance and antigen-negative tumor cells. This study engineered CAR T cells to express IL7 and/or Flt3L and found that IL7 or IL7 Flt3L CAR T cells improved overall survival in tumors pre-conditioned with non-lymphodepleting irradiation. These modified CAR T cells could potentially be combined with other immunotherapies for the treatment of glioblastoma.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Changlin Yang, Guimei Tian, Mariana Dajac, Andria Doty, Shu Wang, Ji-Hyun Lee, Maryam Rahman, Jianping Huang, Brent A. Reynolds, Matthew R. Sarkisian, Duane Mitchell, Loic P. Deleyrolle
Summary: This study reveals the diversity of cell types and dynamic behaviors in glioblastoma, particularly the heterogeneity between slow-cycling cells and cells defined by the expression of stem cell markers. The findings shed light on the potential mechanisms underlying tumor resilience and treatment resistance, and provide insights for the development of precision and effective therapies for brain cancer.
Article
Urology & Nephrology
Chao Gao, Lihe Chen, Enuo Chen, Akaki Tsilosani, Yang Xia, Wenzheng Zhang
Summary: A unique subset of Aqp2(+) cells has been identified as potential progenitor cells for distal renal segments, demonstrating self-renewal, multipotentiality, and clonogenicity abilities to generate various cell types.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Genetics & Heredity
Geoffroy Andrieux, Tonmoy Das, Michaela Griffin, Jakob Straehle, Simon M. L. Paine, Juergen Beck, Melanie Boerries, Dieter H. Heiland, Stuart J. Smith, Ruman Rahman, Sajib Chakraborty
Summary: The study investigated the cell population responsible for 5-aminolevulinic acid (5ALA) metabolism and its role in GBM recurrence. The presence of these cells can serve as an early indicator of recurrence and studying their molecular and cellular features can lead to more effective treatments to delay or block GBM recurrence.
Article
Oncology
Arnon Moldrup Knudsen, Bo Halle, Oriane Cedile, Mark Burton, Christina Baun, Helge Thisgaard, Atul Anand, Christopher Hubert, Mads Thomassen, Signe Regner Michaelsen, Birgitte Brinkmann Olsen, Rikke Hedegaard Dahlrot, Rolf Bjerkvig, Justin Durla Lathia, Bjarne Winther Kristensen
Summary: Surgical tumor resection induces infiltration of microglia/macrophages and elevated expression of pleiotrophin (PTN) in recurrent glioblastoma tumors, promoting therapeutic resistance and tumor recurrence.
Review
Cell & Tissue Engineering
Agnieszka Smolinska, Aleksandra Bzinkowska, Paulina Rybkowska, Magdalena Chodkowska, Anna Sarnowska
Summary: The heterogeneity and unclear developmental origin of MSCs population pose challenges in research and clinical practice. This review article focuses on identifying outstanding subsets of MSCs using specific surface antigens or intracellular markers. The scarce literature available for many candidates mentioned in this article highlights the need for further research, but the collected information can potentially improve the effectiveness of MSC-based therapies.
STEM CELLS INTERNATIONAL
(2023)
Article
Ophthalmology
Jin-Miao Li, Sangbae Kim, Yun Zhang, Fang Bian, Jiaoyue Hu, Rong Lu, Stephen C. Pflugfelder, Rui Chen, De-Quan Li
Summary: This study identified a unique TAC population in limbal basal cells with distinct cell cycle-dependent gene expression profiles. The findings provide insights into the characteristics of ASCs and TACs, laying the foundation for further research on corneal homeostasis and diseases.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Engineering, Biomedical
Jason Wang, Torie Broer, Taylor Chavez, Chris J. Zhou, Sabrina Tran, Yu Xiang, Alastair Khodabukus, Yarui Diao, Nenad Bursac
Summary: This study optimized the methods for deactivating in vitro expanded human myoblasts within a 3D culture of engineered human skeletal muscle tissues. The results showed that a fraction of myoblasts within the culture adopted a quiescent phenotype similar to native satellite cells, and could be reactivated. Single cell RNA-sequencing revealed the existence of two subpopulations of these quiescent and activated cells, and characterized their gene expression changes. This study provides a platform for further research on human muscle regeneration and disease-associated satellite cell dysfunction.
Article
Oncology
Michael C. Burger, Marie-Therese Forster, Annette Romanski, Florian Strassheimer, Jadranka Macas, Pia S. Zeiner, Eike Steidl, Stefanie Herkt, Katharina J. Weber, Jonathan Schupp, Jennifer H. Lun, Maja Strecker, Karolin Wlotzka, Pinar Cakmak, Corinna Opitz, Rosemol George, Iris C. Mildenberger, Paulina Nowakowska, Congcong Zhang, Jasmin Roeder, Elvira Mueller, Kristina Ihrig, Karl-Josef Langen, Michael A. Rieger, Eva Herrmann, Halvard Bonig, Patrick N. Harter, Yvonne Reiss, Elke Hattingen, Franz Roedel, Karl H. Plate, Torsten Tonn, Christian Senft, Joachim P. Steinbach, Winfried S. Wels
Summary: This phase I clinical trial investigated the safety and feasibility of intracranial injection of HER2-targeted CAR-NK cells in patients with recurrent glioblastoma. Results showed that injection of CAR-NK cells was safe and feasible, with no dose-limiting toxicities observed. Some patients showed stable disease after treatment, while others had progressive disease. CD8(+) T-cell infiltration correlated with time to progression.
Article
Biology
Karan Buntval, Hana M. Dobrovolny
Summary: New cancer treatments that minimize patient discomfort are necessary, and one potential therapy is the use of oncolytic viruses. However, it is important to ensure that the viruses only target cancer cells and not harm healthy cells. A mathematical model of viral infection is used to determine the characteristics required for a virus to effectively eliminate a tumor without harming non-cancerous cells.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Biology
Elitsa Golkocheva-Markova, Chiydem Ismailova, Ani Kevorkyan, Ralitsa Raycheva, Sashka Zhelyazkova, Stanislav Kotsev, Maria Pishmisheva, Vanya Rangelova, Asya Stoyanova, Viliana Yoncheva, Tencho Tenev, Teodora Gladnishka, Iva Trifonova, Iva Christova, Roumen Dimitrov, Roberto Bruni, Anna Rita Ciccaglione
Summary: The prevalence of hepatitis E virus (HEV) in the Bulgarian population was underestimated. This study found that the overall seroprevalence of past HEV infection was 10.6%, while the seroprevalence of recent/ongoing HEV infection was 7.5%. Different sub-populations showed different prevalence rates based on gender and age.
Article
Biochemistry & Molecular Biology
Keita Iwasaki, Asuka Araki, C. Murali Krishna, Riruke Maruyama, Tatsuyuki Yamamoto, Hemanth Noothalapati
Summary: Raman spectroscopy has the potential to improve accuracy of cytological and histopathological diagnosis. This study identified five pure biomolecular spectra, with lipid components potentially serving as a basis for discrimination. The technique could become an excellent cytodiagnostic tool for distinguishing breast cancer from normal cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Siddhita A. Jadhav, Pullithadathil Biji, Manoj Kumar Panthalingal, Murali C. Krishna, S. Rajkumar, Dattatraya S. Joshi, Natarajan Sundaram
Summary: The COVID-19 pandemic has highlighted the urgent need for a swift, sensitive, and inexpensive diagnostic protocol. The authors propose a new diagnostic approach based on Surface-enhanced Raman Spectroscopy (SERS) coupled with microfluidic devices to trap and identify viruses from diverse biological fluids. If successful, this approach could serve as a valuable tool for mass screening of COVID-19 and future viral pandemics.
MEDICAL HYPOTHESES
(2021)
Article
Biochemistry & Molecular Biology
Manik N. Waghmare, Tazeen S. Qureshi, C. Murali Krishna, Kshama Pansare, Nikhil Gadewal, Arti Hole, Prabhakar M. Dongre
Summary: The study investigated the influence of the protein-nanoparticle interface on drug binding and stability, demonstrating that the conjugation of whey protein with gold nanoparticles can enhance the binding affinity of anticancer drugs. The interaction mechanisms between drugs and the protein-nanoparticle conjugates were explored, providing insights into the selective conformations induced by drug binding.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Engineering, Biomedical
Atanu Bhattacharjee, Arti Hole, Akshat Malik, Aditi Sahu, S. P. Singh, Atul Deshmukh, Sudhir Nair, Pankaj Chaturvedi, C. Murali Krishna
Summary: The study investigates the potential of Raman spectroscopy in predicting disease-free survival in oral cancer patients, with 76 spectral points identified to accurately predict DFS in oral squamous cell carcinoma. The performance of spectral points was validated through Brier value, indicating a close approximation to actual progression.
LASERS IN MEDICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Neha Kumari, V. L. Mathe, C. Murali Krishna, P. M. Dongre
Summary: The study demonstrated the potential of using BSA conjugates with CIS and GEM anticancer drugs for targeted drug delivery based on different glycations of gp60 protein; UV-visible absorption and fluorescence spectra showed stronger interaction and binding of conjugate 2 and 3 with NANA; Fluorescence quenching data and Raman signal suggested enhanced binding strength of these conjugates with NANA and FUC.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Editorial Material
Oncology
Rohan Chaubal, Sudeep Gupta
INDIAN JOURNAL OF MEDICAL AND PAEDIATRIC ONCOLOGY
(2022)
Article
Instruments & Instrumentation
Dimple Saikia, Priyanka Jadhav, Arti R. Hole, Chilakapati Murali Krishna, Surya P. Singh
Summary: This study demonstrates the application of Raman spectroscopy in monitoring the growth kinetics of three different pathogenic bacteria. The spectral features revealed temporal changes in the peptide and nucleic acid content of cells during different growth stages, and successful discrimination between growth phases was achieved using principal component analysis.
APPLIED SPECTROSCOPY
(2022)
Article
Medical Laboratory Technology
Bharat Rekhi, Leslie Dodd, Bhaskar Dharavath, Amit Dutt
Summary: This study presents one of the first documentations of cytomorphological features of two rare cases of spindle cell/sclerosing rhabdomyosarcoma (RMS). The differential diagnoses and treatment-related implications are discussed.
DIAGNOSTIC CYTOPATHOLOGY
(2022)
Article
Oncology
Asim Joshi, Ashwin Butle, Supriya Hait, Rohit Mishra, Vaishakhi Trivedi, Rahul Thorat, Anuradha Choughule, Vanita Noronha, Kumar Prabhash, Amit Dutt
Summary: This study found that osimertinib showed significant benefit in lung cancer patients with low allele frequency of EGFR T790M mutation, and its therapeutic effect was confirmed through experiments.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Spectroscopy
Priyanka A. Jadhav, Kshama Pansare, Arti Hole, Arvind Ingle, Rukmini Govekar, C. Murali Krishna
Summary: About two thirds of oral cancer cases are diagnosed at advanced stages, highlighting the importance of early screening. This study used an animal model to investigate early biochemical changes during DMBA-induced oral carcinogenesis, and found that Raman spectroscopy can be an effective tool for early diagnosis. The findings suggest that serum RS can detect increasing protein and loss of lipid content as oral carcinogenesis progresses.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Multidisciplinary Sciences
Aditi Sahu, Kivanc Kose, Lukas Kraehenbuehl, Candice Byers, Aliya Holland, Teguru Tembo, Anthony Santella, Anabel Alfonso, Madison Li, Miguel Cordova, Melissa Gill, Christi Fox, Salvador Gonzalez, Piyush Kumar, Amber Weiching Wang, Nicholas Kurtansky, Pratik Chandrani, Shen Yin, Paras Mehta, Cristian Navarrete-Dechent, Gary Peterson, Kimeil King, Stephen Dusza, Ning Yang, Shuaitong Liu, William Phillips, Pascale Guitera, Anthony Rossi, Allan Halpern, Liang Deng, Melissa Pulitzer, Ashfaq Marghoob, Chih-Shan Jason Chen, Taha Merghoub, Milind Rajadhyaksha
Summary: The response to immunotherapies is unpredictable and variable. Traditional pathology-based phenotyping of tumors is unable to accurately predict treatment response to immunotherapy. Researchers have developed a non-invasive in vivo imaging technique using reflectance confocal microscopy to combine inflammation profiles with vascular features, allowing for dynamic cellular-level phenotyping of the tumor immune microenvironment. This approach provides a more reliable predictor of response to immune therapy in skin cancer patients.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Dimple Saikia, Priyanka Jadhav, Arti R. Hole, Chilakapati Murali Krishna, Surya P. Singh
Summary: This study applied Raman spectroscopy to investigate the differences between colistin sensitive and resistant bacterial strains, and successfully generated colistin resistant clones. A unique spectral profile associated with specific drug concentration was obtained, allowing for successful differentiation between resistant and sensitive cells. These findings support the prospective utility of Raman spectroscopy in identifying antimicrobial resistance.
Article
Chemistry, Multidisciplinary
Sukanya Rauniyar, Kshama Pansare, Asmita Sharda, Saurav Raj Singh, Panchali Saha, Murali Krishna Chilakapati, Sanjay Gupta
Summary: Investigating the effect of sub-culturing in parental and radioresistant MCF-7 cells, this study found that radioresistant cells showed high-intensity nucleic acid and cytochrome bands, potentially serving as a radiation-induced spectral marker. Raman spectroscopy data revealed dynamic biochemical alterations in revived radioresistant G(2)/M synchronized cells at early cell passages, stabilizing at a later stage. The importance of cell passaging and cell cycle phases in potentially altering biochemical parameters in radioresistant cells post-cryopreservation was highlighted.
Article
Otorhinolaryngology
Panchali Saha, Sharada Sawant, Atul Deshmukh, Arti Hole, C. Murali Krishna
Summary: This study evaluates the potential of serum Raman spectroscopy (SRS) in identifying recurrence-prone oral cancer subjects. DNA and protein alterations were observed in serum after surgery for all groups. Stratification of post-surgery, recurrence, and non-recurrence subjects was achieved with high accuracy (84%) using SRS, while no stratification was observed before surgery.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2023)
Article
Mathematical & Computational Biology
Sanket Desai, Rohit Mishra, Suhail Ahmad, Supriya Hait, Asim Joshi, Amit Dutt
Summary: This article discusses the impact of the lack of Indian-specific reference germline variation resources on identifying true cancer-associated somatic variants among Indian cancer patients, and the method and results of generating the TMC-SNPdb 2.0 database by integrating different studies.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2022)