Article
Chemistry, Analytical
Ya-ru Meng, Dandan Zhang, Xiaoran Zou, Fei Ma, Qi Kang, Chun-yang Zhang
Summary: Developed a label-free and sensitive method for NAD(+) assay using a trifunctional split dumbbell probe and ligation-triggered isothermal rolling circle amplification. The method exhibits high sensitivity with a detection limit of 85.6 fM, good selectivity, and potential applications in biological research and clinical diagnosis related to NAD(+).
Article
Chemistry, Analytical
Elena V. Suprun, Gulnaz R. Kutdusova, Svetlana A. Khmeleva, Konstantin G. Ptitsyn, Leonid K. Kurbatov, Sergey P. Radko
Summary: In this study, the electrochemical behavior of nucleic acids was investigated using synthetic DNA oligonucleotides and single-stranded DNA amplification products. The oxidation of guanine (dG) and adenine (dA) residues in the oligonucleotides was studied by square wave and cycling voltammetry. The results showed that the oxidation process of dissolved oligonucleotides was diffusion-controlled, while adsorbed oligonucleotides demonstrated adsorption-limited responses. The signal intensity of the oligonucleotides exponentially decreased with the molecule length at a constant concentration. The oxidation signal of dG in G-n oligonucleotides was insensitive to the position of dG, but showed a bell-shaped dependence on the amount of dG. The oxidation signal of dG in A-n oligonucleotides increased with the number of dA residues in a bell-shaped fashion. Folding the oligonucleotides into hairpins shifted the peak potential for dG oxidation to higher values.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Jun Zhao, Jiandong Wu, Xiaosong Wu, Guoqing Deng, Yong Liu, Francis Lin, Ling Zhu
Summary: An efficient self-quenching fluorescence probe and the corresponding detection system were designed, capable of achieving a wide dynamic range and low detection limit in a single step. The method, with high specificity and biocompatibility, has great potential for accurately quantifying miRNA biomarkers in serum, providing promising prospects for clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Yuehua Wang, Yingli Han, Fangyu Zhou, Tingting Fan, Feng Liu
Summary: DNA methyltransferases can be used as biomarkers for genetic diseases. A padlock probe was designed to detect the activity of DNA methyltransferases without the need for purification or other probe assistance. This method has high sensitivity and a wide linear range, and can be applied in complex biological environments, as well as for screening drugs or inhibitors for methyltransferases.
Article
Chemistry, Analytical
Yanheng Yao, Chengjie Duan, Yan Chen, Zhiqiang Hou, Wenting Cheng, Dayong Li, Zhongyun Wang, Yang Xiang
Summary: A fluorescent biosensor for detecting lncRNA HULC was developed based on rolling circle amplification induced by multi-primer probes. The method showed high selectivity and sensitivity, providing a new approach for translating specific lncRNA into clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Bingzhi Li, Anqi Xia, Siying Xie, Lei Lin, Zhirun Ji, Tiying Suo, Xing Zhang, He Huang
Summary: A signal-amplified detection method of FEN1 based on cleavage-induced ligation and rolling circle amplification was developed in this study, achieving highly sensitive and specific detection of FEN1 in real samples, as well as evaluating the inhibitory effects of chemicals on FEN1. This novel biosensor method enriches the tools for FEN1-based diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Su Jiang, Meng Liu, Wei Tantai, Qinfeng Xu, Xiaoran Zou, Fei Ma, Chun-yang Zhang
Summary: We have developed an aptamer-mediated rolling circle amplification approach for the label-free and sensitive detection of histone-modifying enzyme activity. This method can achieve femtomolar sensitivity for the assay of histone acetyltransferase Tip60, and can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Yuhan Wang, Jiaxuan Xiao, Xiaona Lin, Amira Waheed, Ayyanu Ravikumar, Zhen Zhang, Yanmin Zou, Chengshui Chen
Summary: A sensitive biosensor based on rolling circle amplification (RCA) was developed for colorimetric quantification of lead ion (Pb2+). The biosensor utilized GR5 DNAzymes modified on immunomagnetic beads to capture Pb2+ and initiate an RCA for improved sensitivity. The biosensor also generated a G4-hemin DNAzyme as a colorimetric signal, which showed potential in rapid detection of heavy metals in the environment.
Article
Chemistry, Multidisciplinary
Lei He, Xiaocheng Yu, Rongrong Huang, Lian Jin, Yuan Liu, Yan Deng, Song Li, Hui Chen, Zhu Chen, Zhiyang Li, Pengfeng Xiao, Nongyue He
Summary: A highly specific and sensitive fluorescence scheme based on padlock probe-based exponential rolling circle amplification (P-ERCA) assay for detecting extracellular vesicles derived from lung cancer cells was reported. The method exhibits high specificity and sensitivity, and successfully distinguishes between lung cancer patients and healthy individuals in real human serum samples.
Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Ruijin Zeng, Jun Wang, Qingshui Wang, Dianping Tang, Yao Lin
Summary: A versatile colorimetric biosensing platform was developed for sensitive, specific, and rapid screening of cancer-derived exosomes using horseradish peroxidase-encapsulated DNA nanoflowers (HRP-DF) as biorecognition elements and signal-generation tags. The platform showed satisfactory colorimetric responses towards target exosomes with high accuracy, strong anti-interference ability, and good reproducibility.
Article
Chemistry, Analytical
Jingjing Qian, Yixia Yang, Feng Gong, Xiaoyun Shan, Xinghu Ji, Zhike He
Summary: A novel method combining NanoCluster Beacon (NCB) and rolling cycle amplification (RCA) was developed for ATP detection. NCB was composed of silver clusters (P2-AgNCs), a converter (P3), and a linker sequence (P1) that kept them in close proximity. The fluorescence performance of NCB correlated with the status of the AgNCs, allowing controllable color switching from green to red. Under optimized conditions, the ratio of fluorescence intensities at 630 nm and 542 nm (F630 nm/F542 nm, F630/542) showed a linear correlation with the logarithmic ATP concentration, with a detection limit of 122 pM. The proposed method also demonstrated good performance in human serums, indicating its potential for clinical diagnosis.
MICROCHEMICAL JOURNAL
(2023)
Article
Biophysics
Feng Chen, Guodong Li, Chun Wu, Wanhe Wang, Dik-Lung Ma, Chung-Hang Leung
Summary: In this study, a rapid and label-free interference reduction nucleic acid amplification strategy (IR-NAAS) was developed for diagnosing COVID-19 RNAs sequences. It utilized luminescent iridium(III) probes, time-resolved emission spectroscopy (TRES), and multi-branch rolling circle amplification (mbRCA) to achieve high sensitivity and specificity. The method was successfully applied to detect COVID-19 RNA sequences in various environmental and biological samples, and also showed potential for detecting other RNA viruses.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Rongfeng Cai, Shixin Zhang, Luo Chen, Maolin Li, Yuting Zhang, Nandi Zhou
Summary: A dual signal amplification electrochemical biosensor based on a DNA walker and DNA nanoflowers was constructed for high sensitivity detection of Staphylococcus aureus. Through nucleic acid hydrolysis and rolling circle amplification reaction, sensitive detection of S. aureus with a detection limit of 9 CFU/mL was achieved.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Yanhu Wang, Mengchun Yang, Huihui Shi, Shenguang Ge, Xiao Wang, Jinghua Yu
Summary: A sensitive photoelectrochemical (PEC) biosensing platform was demonstrated for exosomal miRNA assay using lambda-exonuclease for amplification strategy, while a target exosomal miRNA-activatable release nanocarrier was fabricated to enable signal control. This study provides a feasible approach for the efficient diagnosis and prognosis prediction of diseases based on the detection of exosomal miRNAs-21.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xiaoran Tan, Haihan Yu, Bing Liang, Mengting Han, Shenguang Ge, Lina Zhang, Lin Li, Li Li, Jinghua Yu
Summary: In this study, a paper-based photoelectrochemical (PEC) sensor was developed for ultrasensitive detection of OTA. By using polyvinylpyrrolidone-treated In2S3/WO3 functionalized paper as a signal transducer and employing the Exo III-assisted cycling process and PECCC redox cycling strategy, the sensor achieved high sensitivity in OTA detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Hongmei Yang, Jiajun Wang, Haihan Yu, Xiao Li, Zhenglin Li, Kang Cui, Lina Zhang, Shenguang Ge, Jinghua Yu
Summary: A simple yet effective photocathodic signal enhancement strategy was developed based on FeOOH/Cu2O/CuS photocathode for lab-on-paper homogeneous PEC bioassay, achieving efficient charge carrier separation and electron acceptor reduction. This work provides practical guidelines for developing high-performance lab-on-paper cathodic PEC bioanalysis systems.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Physical
Yunqing Liu, Xuefeng Wang, Yuanna Zhu, Haiqing Wang, Jinghua Yu, Hong Liu, Shenguang Ge
Summary: NiFeMoOx nanosheets with high-performance OER activity were prepared by promoting the oxidation cycle process in NiFe oxyhydroxide through high-valence Mo doping. The resulting nanosheets exhibited outstanding alkaline OER activity and long-term stability.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Mengsu Hu, Jiajun Wang, Jiangxue Han, Yumeng Rong, Haihan Yu, Shenguang Ge, Hongmei Yang, Lina Zhang, Jinghua Yu
Summary: In this study, a new paper-based photoelectrochemical biosensor was developed for microRNA determination using the cascaded sensitization effect of ZnO/CuInS2/Ag2Se photoactive structures and a nuclease-propelled target dual-recycling amplification strategy. The biosensor achieved ultrasensitive detection of miRNA-141 with high accuracy and selectivity, demonstrating great potential for developing high-performance microfluidic paper analytical devices.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Xuefeng Wang, Fengyi Wang, Jianrong Wang, Yunqing Liu, Chaomin Gao, Shenguang Ge, Jinghua Yu
Summary: Herein, a highly sensitive and specific photoelectrochemical biosensor is constructed using CRISPR/Cas12a and catalytic hairpin assembly (CHA) non-enzyme amplification technology. The sensor, using Bi/g-C3N4 nanohybrids as a composite electrode, demonstrates accurate detection of miRNA-122 with the help of multiple amplification mechanisms. The universal CRISPR/Cas12a cutting characteristics further expand its analysis potential and simplify the operation difficulty, showing great potential in biomedical detection and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Qingxin Zhang, Manru Zhang, Zhiqiang Guo, Jingjing Li, Zhixue Zhu, Yu Wang, Su Liu, Jiadong Huang, Jinghua Yu
Summary: In this study, a low-background electrochemical biosensor based on a DNA tetrahedron-besieged primer and a DNAzyme-activated programmatic rolling circle amplification (RCA) was proposed for OTA detection in wine samples. The low-background detection was achieved by using the besieged primer and the target OTA-mediated DNAzyme activation initiated the programmatic RCA. The integration of the catalytic property of silver nanoclusters (AgNCs) with the electrochemical assay provided high sensitivity for OTA detection. This novel design showed great potential in food safety and environmental detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Xu Li, Jiali Huang, Jiayu Ding, Mingzhen Xiu, Kang Huang, Kang Cui, Jing Zhang, Shiji Hao, Yan Zhang, Jinghua Yu, Yizhong Huang
Summary: In this study, a dual-mode lab-on-paper device was constructed using BiVO4/FeOOH nanocomposites as an efficient photoelectrochemical (PEC)/colorimetric signal reporter for sensitive detection of carcinoembryonic antigen (CEA). The nanocomposites with an FeOOH needle-like structure displayed enhanced PEC response activity, enabling quantitative detection of CEA with the PEC signal readout mode. Additionally, a colorimetric signal readout system was achieved through a smart folding technique, providing a dual-mode signal readout for the lab-on-paper device.
Article
Chemistry, Analytical
Qian Wang, Liang Lv, Wenhao Chi, Yujiao Bai, Wenqing Gao, Peihua Zhu, Jinghua Yu
Summary: A new porphyrin-based porous covalent organic framework (TAP-COF) with high surface areas and efficient electron transport was synthesized. The TAP-COF exhibited excellent peroxidase-like catalytic activity and was able to catalyze the oxidation of a substrate by hydrogen peroxide. Simple and rapid colorimetric methods for in situ hydrogen peroxide detection were developed using this TAP-COF nanozyme, which has potential applications in point-of-care testing, cell biology, and clinical diagnosis.
Article
Chemistry, Analytical
Huihui Shi, Yanfei Che, Yumeng Rong, Jiajun Wang, Yanhu Wang, Jinghua Yu, Yan Zhang
Summary: An integrated dual-signal bioassay was developed for ultrasensitive detection of thrombin. This method combined visualization with photoelectrochemical technique to achieve the detection. The bioassay demonstrated wide linear ranges, high selectivity, stability, and reproducibility under optimized conditions. Additionally, the proposed visual/photoelectrochemical transduction mechanism platform offered a lower background signal and more reliable detection results, providing an effective way for detecting other proteins and nucleic acids.
Article
Chemistry, Analytical
Wenli Xiu, Peini Zhao, Yujie Pan, Xiaoru Wang, Lina Zhang, Shenguang Ge, Jinghua Yu
Summary: A surface-enhanced Raman scattering (SERS) sensor was developed for rapid on-site determination of ethephon in food, based on the folding and assembly characteristics of paper fibers, the skeleton controllability of metal-organic framework materials (MOFs), and the morphology designability of plasmonic noble metal materials. The sensor used HKUST-1(Cu) assembled onto a carbon-treated chromatographic paper matrix to overcome poor affinity of SERS substrate for target molecules. With the coupling of biomimetic antibodies and a multi-stage progressive enhancement structure, the sensor achieved high specificity and sensitivity for ethephon detection with a linear range of 10-3 to 10 mg kg-1 and a low detection limit of around 1.39 x 10-4 mg kg-1.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Li Shan, Yuanyuan Chen, Xiaoran Tan, Shenguang Ge, Lina Zhang, Lin Li, Jinghua Yu, Li Li
Summary: A newly designed two-in-one tetrahedral DNA (TDN) nanostructure with an antifouling surface and backbone-rigidified interfacial tracks was developed for highly sensitive and selective detection of miRNA-182-5p. The TDN-modified sensing interfaces demonstrated incomparable antifouling ability against protein samples and interfering miRNAs due to the strong hydrophilic capacity and special molecular conformations. The designed TDN-modified paper-based photoelectro-chemical sensor successfully quantified miRNA-182-5p with a low detection limit of 0.09 fM and high specificity in real samples, showing great promise for bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Shanshan Li, Haihan Yu, Yuwen Wang, Shuai Wang, Lina Zhang, Peihua Zhu, Chaomin Gao, Jinghua Yu
Summary: A stable organic-inorganic Z-scheme heterostructure was constructed by covalently connecting COF-366-Fe and CeO2 through Schiff base reaction, which effectively integrated their respective properties and improved the separation and transfer of photoexcited carriers. The COF-366-Fe@CeO2 exhibited superior photocatalytic CO yield compared to COF-366-Fe and CeO2, indicating its potential as a highly-effective COF-based photocatalyst for artificial photosynthesis.
APPLIED SURFACE SCIENCE
(2023)
Article
Biophysics
Lin Li, Hui Tian, Guofu Wang, Suyue Ren, Tinglei Ma, Yixiang Wang, Shenguang Ge, Yan Zhang, Jinghua Yu
Summary: Ready-to-use in vitro diagnosis is crucial for cervical cancer prevention and treatment. A paper-film-based differential lateral flow biosensor is proposed, allowing simultaneous detection of two high-risk HPV types. The biosensor utilizes a CuCo2S4/ZnIn2S4 heterostructure on a paper-film chip and introduces surface sulfur vacancy for enhanced photoexcitation. Through sequential solution transfer, the biosensor activates the self-assembly of DNA and the cleavage activity of CRISPR-Cas12a, resulting in the release of polydopamine-coated gold nanoparticles. This intelligent biosensor achieves low limit-of-detection for HPV-18 and HPV-16 and provides a promising approach for home medical intelligent diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
