Article
Chemistry, Analytical
Mengjiao Huang, Lin Zhu, Siyin Kang, Fude Chen, Xinyu Wei, Liyuan Lin, Xiaofeng Chen, Wei Wang, Zhi Zhu, Chaoyong Yang, Yanling Song
Summary: The study developed a FRET-based strategy for visualizing PD-L1-specific glycosylation, allowing observation of PD-L1-specific glycosylation on tissue sections while preserving spatial context and tissue structure. This method revealed the correlation between tissue level of PD-L1-specific glycosylation and the efficacy of PD-1/PD-L1 immunotherapy, offering a new potential for predicting immunotherapy response.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yutaro Kondo, Susumu Suzuki, Shoya Ono, Mitsuo Goto, Satoru Miyabe, Tetsuya Ogawa, Hiromi Tsuchida, Hideaki Ito, Taishi Takahara, Akira Satou, Toyonori Tsuzuki, Kazuhiro Yoshikawa, Ryuzo Ueda, Toru Nagao
Summary: The expression of PD-L1 is regulated by complex mechanisms. This study focused on the mechanisms of PD-L1 expression in OSCCs and found that EGF and IFN-gamma upregulate PD-L1 through different signaling pathways. Moreover, the phosphorylation pattern of related molecules in PD-L1-positive cells varied among different cases. These findings suggest that choosing the appropriate combination drugs for PD-1 blockade cancer treatment should consider the tissue environment and molecular alterations affecting PD-L1 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Jill Jasmine Dalimot, Ilka Mc Cormick Smith, Jasmin Gerkrath, Sylvia Hartmann, Oliver A. Cornely, Soo Chan Lee, Joseph Heitman, Volker Rickerts
Summary: This study focused on improving the accuracy of mucormycosis diagnosis from tissue samples using fluorescence in situ hybridization (FISH) targeting specific regions of fungal ribosomal RNA (rRNA) of Mucorales. While the probe combination showed success in targeting Mucorales and enhancing diagnosis, autofluorescence and variations in physiological states of fungi and antifungal therapy may impact the FISH signal obtained. Further studies are needed to determine the value of FISH in diagnosing mucormycosis from different clinical samples.
Article
Dentistry, Oral Surgery & Medicine
Shuang-Bo Xu, Meng-Yao Wang, Xin-Zhan Shi, Qiong Wang, Miao Yu, Wei Zhang, Xiao-Hui Xu, Lai-Kui Liu
Summary: The study found that PD-1 and PD-L1 may induce immune suppression in oral leukoplakia (OLK) and accelerate the progress of malignant transformation.
Article
Multidisciplinary Sciences
Sebastian Blatt, Maximilian Krueger, Constantin Rump, Stefanie Zimmer, Keyvan Sagheb, Julian Kuenzel
Summary: PD-L1 expression varies between oral and oropharyngeal squamous cell carcinoma, but does not affect patient survival. The status of p16 is also unrelated to PD-L1 expression.
Article
Biochemistry & Molecular Biology
Sonja Steen, Karl Semmelmayer, Christa Flechtenmacher, Juergen Hoffmann, Kolja Freier, Dominik Horn, Jochen Hess, Christian Freudlsperger, Julius Moratin
Summary: The introduction of immune checkpoint inhibition has provided a new treatment option for advanced oral cancers. However, there is a lack of data on the dynamic regulation of PD-L1 expression in recurrent tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
I-Tsang Chiang, Yuan-Hao Lee, Zhao-Lin Tan, Fei-Ting Hsu, Hsi-Feng Tu
Summary: Combination therapy with regorafenib and anti-PD-L1 can enhance anti-tumor immune response and suppress immunosuppression in oral squamous cell carcinoma (OSCC). This finding contributes to the development of more effective treatment strategies for OSCC.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Oncology
Torsten Goldmann, Sebastian Marwitz, Dorte Nitschkowski, Rosemarie Krupar, Max Backman, Hedvig Elfving, Viktoria Thurfjell, Amanda Lindberg, Hans Brunnstrom, Linnea La Fleur, Artur Mezheyeuski, Johanna Sofia Margareta Mattsson, Johan Botling, Patrick Micke, Carina Strell
Summary: The study reveals that PD-L1 amplification correlates with PD-L1 expression in squamous cell cancer and is associated with a tumor phenotype rich in immune cells. However, the correlation between PD-L1 amplification and PD-L1 protein expression is weak, and in some cases, PD-L1 protein may be negative despite amplification.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Kit Kitichotkul, Nirush Lertprasertsuke, Sompid Kintarak, Surawut Pongsiriwet, Warit Powcharoen, Anak Iamaroon
Summary: This study investigated the expression of PD-L1 and its associations with HPV infection, p16 expression, and other clinical characteristics in patients with OSCC. The results showed that approximately one-fourth of OSCC patients were PD-L1-positive, and there was no association between HPV infection or p16 expression and PD-L1 expression.
Article
Biochemical Research Methods
Yixin Fu, Husun Qian, Xi Zhou, You Wu, Lin Song, Kena Chen, Dan Bai, Yujun Yang, Junjie Li, Guoming Xie
Summary: This study presents a proximity ligation assay mediated rolling circle amplification (PLA-RCA) strategy for amplified imaging of glycosylated PD-L1 in situ. The feasibility of the strategy has been validated by visualizing PD-L1 glycosylation in different cancer cells and monitoring the variation of PD-L1 glycosylation during drug treatment. This work offers a useful alternative to track protein-specific glycosylation and potentially advances the investigation of the dynamic glycan state associated with the disease process.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Oncology
Antian Gao, Xiao Pan, Xudong Yang, Zitong Lin
Summary: This review discusses the advantages of anti-PD-1/PD-L1 therapy for oral squamous cell carcinoma (OSCC) patients, and identifies three aspects currently available for predicting treatment efficacy.
INVESTIGATIONAL NEW DRUGS
(2021)
Article
Nanoscience & Nanotechnology
Yushi Wang, Yingying Chen, Yeqing Wan, Chen Hong, Jinhua Shang, Fengzhe Li, Xiaoqing Liu, Fuan Wang
Summary: This study proposes an autocatalytic hybridization system that combines hybridization chain assembly and catalytic DNA assembly for highly efficient positive feedback amplification. The system allows sensitive detection of polynucleotide kinase and has the potential for accurate monitoring and screening in vitro and in biological samples.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Yixin Liu, Binxiao Li, Ya-Jun Wang, Zihui Fan, Yang Du, Bin Li, Yan-Jun Liu, Baohong Liu
Summary: In this study, enzyme-free-mediated cascade amplified nanoprobes were designed for in situ single-molecule imaging of dual-microRNAs in switchable migrating cells. The differential expression and localization of dual-miRNAs were clearly exhibited in multiple cell lines due to the enhanced sensitivity via the cascade signal amplification strategy. Moreover, in situ three-dimensional imaging of dual-miRNAs in transition of cell migration phenotypes was successfully reconstructed in both non-confined and confined microenvironments in vitro, demonstrating differential spatial distribution in a single cell. This holds great promise for exploring the key roles of spatial RNA distribution at the single-molecule level in migrating cells, and for advancing the understanding of molecular mechanisms and physical principles in 3D cell migration in vivo.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Lin Yang, Shousheng Liu, Wenzhuo He, Zhenchong Xiong, Liangping Xia
Summary: This study evaluated PD-L1 genetic alterations in patients with colorectal cancer (CRC) and analyzed their correlation with tumor immune microenvironment and clinical implications. The prevalence of PD-L1 genetic alterations was relatively low in CRC, but they were associated with aggressive features. The correlation between PD-L1 genetic alterations and tumor immune features was only observed in mismatch repair-deficient (dMMR) CRC.
Article
Immunology
Tiantian Wu, Caijin Tang, Renchuan Tao, Xiangzhi Yong, Qiaozhi Jiang, Cong Feng
Summary: The research found that PD-L1 is highly expressed in oral squamous cell carcinoma (OSCC), correlated with worse survival, and related to macrophage infiltration and EMT process. Patients with high PD-L1 expression may benefit from ICI treatment and show higher sensitivity to certain chemical drugs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Michael Behnes, Shqipdona Lahu, Gjin Ndrepepa, Maurizio Menichelli, Katharina Mayer, Jochen Woehrle, Isabell Bernlochner, Senta Gewalt, Bernhard Witzenbichler, Willibald Hochholzer, Dirk Sibbing, Salvatore Cassese, Dominick J. Angiolillo, Rayyan Hemetsberger, Christian Valina, Arne Mueller, Sebastian Kufner, Christian W. Hamm, Erion Xhepa, Alexander Hapfelmeier, Hendrik B. Sager, Michael Joner, Massimiliano Fusaro, Gert Richardt, Karl-Ludwig Laugwitz, Franz-Josef Neumann, Heribert Schunkert, Stefanie Schupke, Adnan Kastrati, Ibrahim Akin
Summary: This analysis compared the efficacy and safety of ticagrelor and prasugrel in patients with acute coronary syndrome (ACS) presenting during different time periods.
CLINICAL RESEARCH IN CARDIOLOGY
(2023)
Article
Anesthesiology
Phillip Hoppe, Christian Burfeindt, Philip C. Reese, Luisa Briesenick, Moritz Flick, Karim Kouz, Hans Pinnschmidt, Alexander Hapfelmeier, Daniel Sessler, Bernd Saugel
Summary: This study found that chronic arterial hypertension and nocturnal blood pressure non-dipping are independent risk factors for postinduction and intraoperative hypotension in non-cardiac surgery patients. Adding information on these factors improves the accuracy of hypotension prediction models based on preoperative clinical information.
JOURNAL OF CLINICAL ANESTHESIA
(2022)
Article
Pharmacology & Pharmacy
Christina Irene Guenter, Felicitas Paula Ilg, Alexander Hapfelmeier, Silvia Egert-Schwender, Wolfgang Jelkmann, Shibashish Giri, Augustinus Bader, Hans-Guenter Machens, EPO Burns Study Grp
Summary: Burns are a major cause of severe illness and death. EPO may have a positive effect on wound healing in burns, but further clinical trials are needed to confirm this.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pathology
Moritz Jesinghaus, Anna-Lina Herz, Meike Kohlruss, Miguel Silva, Albert Grass, Sebastian Lange, Alexander Novotny, Katja Ott, Thomas Schmidt, Matthias Gaida, Alexander Hapfelmeier, Carsten Denkert, Wilko Weichert, Gisela Keller
Summary: Tumour budding (TB) in gastric adenocarcinoma (GC) is associated with adverse clinicopathological factors and poor survival. The assessment of TB according to the criteria from the International Tumour Budding Consensus Conference (ITBCC) provides valuable prognostic information in GCs resected after neoadjuvant therapy.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2022)
Article
Behavioral Sciences
Ana Klein, Toni Christoph Berger, Alexander Hapfelmeier, Matthias Schaffert, William Matuja, Erich Schmutzhard, Andrea S. Winkler
Summary: The presence of a neurologist in epilepsy clinics in Tanzania can significantly reduce the number of seizures experienced by patients per month but may also increase the occurrence of side effects of medication. Specialist training and relevant infrastructure should be encouraged in low-income and middle-income countries.
EPILEPSY & BEHAVIOR
(2023)
Article
Oncology
Theresa Hiltner, Noemi Szoerenyi, Meike Kohlruss, Alexander Hapfelmeier, Anna-Lina Herz, Julia Slotta-Huspenina, Moritz Jesinghaus, Alexander Novotny, Sebastian Lange, Katja Ott, Wilko Weichert, Gisela Keller
Summary: This study analyzed the allelic imbalance (AI) of major histocompatibility complex (MHC) class I genes in patients with gastric/gastroesophageal adenocarcinoma and its association with clinical outcome. The results showed that AI was significantly associated with response or survival, particularly AI at markers of the HLA region was associated with decreased survival in responding patients. These findings highlight the importance of intact neoantigen presentation specifically for responding patients and may help explain the poor survival despite tumor regression after neoadjuvant CTx.
Article
Cardiac & Cardiovascular Systems
Alp Aytekin, J. J. Coughlan, Gjin Ndrepepa, Salvatore Cassese, Shqipdona Lahu, Sebastian Kufner, Katharina Mayer, Erion Xhepa, Senta Gewalt, Michael Joner, Alexander Hapfelmeier, Dominick J. Angiolillo, Maurizio Menichelli, Gert Richardt, Franz J. Neumann, Heribert Schunkert, Adnan Kastrati
Summary: This study analyzed the treatment effect of a prasugrel vs. a ticagrelor based strategy on total ischaemic and bleeding events in patients with ACS. The results showed that prasugrel was associated with a lower risk of myocardial infarction compared to ticagrelor, but the risk of bleeding events was similar between the two groups. Confirmation of these findings through future studies is important.
EUROPEAN HEART JOURNAL-CARDIOVASCULAR PHARMACOTHERAPY
(2023)
Article
Computer Science, Interdisciplinary Applications
Alexander Hapfelmeier, Roman Hornung, Bernhard Haller
Summary: Hypothesis testing of variable importance measures (VIMPs) is an ongoing research topic. Different approaches have been proposed to address the computational costs associated with these tests, including heuristic approaches, formal tests, and non-parametric permutation tests. The use of sequential permutation tests and sequential p-value estimation can significantly reduce the computational costs of conventional permutation tests. Simulation studies and application studies confirm the theoretical properties and numerical stability of these methods.
COMPUTATIONAL STATISTICS & DATA ANALYSIS
(2023)
Article
Psychiatry
Antonius Schneider, Laura Huber, Jorg Lohse, Klaus Linde, Anna Greissel, Heribert Sattel, Peter Henningsen, Alexander Hapfelmeier
Summary: This study aimed to investigate the association of possible somatic symptom disorder (SSD), depression, anxiety, and participant-reported symptoms with daily life impairment (DLI). The results showed a strong association between DLI and self-reported fatigue, dyspnea, impaired concentration, SSD-12, and PHQ-2. This suggests that PCS is a complex clinical picture in which SSD may play an important role when DLI is present.
JOURNAL OF PSYCHOSOMATIC RESEARCH
(2023)
Article
Economics
Johannes Wendl, Andreas Simon, Martin Kistler, Jana Hapfelmeier, Antonius Schneider, Alexander Hapfelmeier
Summary: This study aims to overcome methodological limitations in current research on medication adherence and its relationship to healthcare costs. Using different modeling approaches, the study found a positive association between medication adherence and total costs, a weak association with outpatient costs, positive association with pharmacy costs, and frequently negative association with inpatient costs. The study highlights the importance of avoiding concurrent measurement and considering the presence of a non-linear relationship.
APPLIED HEALTH ECONOMICS AND HEALTH POLICY
(2023)
Article
Medicine, General & Internal
Silvia Wuerstle, Alexander Hapfelmeier, Siranush Karapetyan, Fabian Studen, Andriana Isaakidou, Tillman Schneider, Roland M. Schmid, Stefan von Delius, Felix Gundling, Rainer Burgkart, Andreas Obermeier, Ulrich Mayr, Marc Ringelhan, Sebastian Rasch, Tobias Lahmer, Fabian Geisler, Paul E. Turner, Benjamin K. Chan, Christoph D. Spinner, Jochen Schneider
Summary: Ascitic fluid infection is a serious complication of liver cirrhosis, and it is crucial to distinguish between spontaneous bacterial peritonitis (SBP) and secondary peritonitis in these patients due to different treatment approaches. This study analyzed 532 SBP episodes and 37 secondary peritonitis episodes to identify key differentiation criteria. Microbiological characteristics, severity of illness, and clinicopathological parameters were identified as the most important predictors to distinguish between SBP and secondary peritonitis. A point-score model with ten discriminatory features was established, and two cut-off scores were defined to divide patients into low-risk and high-risk groups for secondary peritonitis based on a sensitivity of 95% to rule out or rule in SBP episodes. Overall, the discrimination between secondary peritonitis and SBP remains challenging, but the findings of this study may help clinicians with the crucial differentiation.
Article
Cardiac & Cardiovascular Systems
Benjamin Brunn, Alexander Hapfelmeier, Rudolf A. Jorres, Konrad Schultz, Antonius Schneider
Summary: By combining FeNO with clinical signs and symptoms, the diagnostic value of nitric oxide in ruling-in and ruling-out asthma can be increased. A new model has been developed for this purpose.
RESPIRATORY MEDICINE
(2023)
Article
Clinical Neurology
Alexander Hapfelmeier, Begum Irmak On, Mark Muehlau, Jan S. Kirschke, Achim Berthele, Christiane Gasperi, Ulrich Mansmann, Alexander Wuschek, Matthias Bussas, Martin Boeker, Antonios Bayas, Makbule Senel, Joachim Havla, Markus C. Kowarik, Klaus Kuhn, Ingrid Gatz, Helmut Spengler, Benedikt Wiestler, Lioba Grundl, Dominik Sepp, Bernhard Hemmer
Summary: The study aims to support treatment decision-making for patients with early RRMS and CIS by using an algorithm to create a treatment decision score (MS-TDS). By integrating multiple data sources, the MS-TDS predicts the probability of new or enlarging lesions in cerebral MRIs between 6 and 24 months after the initial MRI. The results show that the MS-TDS can estimate individualized treatment success probabilities and identify patients who benefit from early platform medication.
THERAPEUTIC ADVANCES IN NEUROLOGICAL DISORDERS
(2023)
Article
Education, Scientific Disciplines
Anna-Lena Blaschke, Alexander Hapfelmeier, Hannah P. K. Rubisch, Pascal O. Berberat, Martin Gartmeier
Summary: Bedside teaching is a crucial element in medical education, and our study investigated the influence of teacher and structural characteristics on its perceived quality. We found that the intrinsic motivation of the teachers was positively correlated with quality, while experience had a weaker correlation. Among the structural characteristics, the number of patients and the proportion of time spent on clinical examination were significantly associated with the quality dimension of clinical teaching.
Article
Medicine, General & Internal
Antonius Schneider, Benjamin Brunn, Alexander Hapfelmeier, Konrad Schultz, Christina Kellerer, Rudolf A. Jorres
Summary: This study validates the diagnostic accuracy of fractional exhaled nitric oxide (FeNO) for diagnosing asthma, and finds that higher FeNO values are associated with increased responsiveness to inhaled corticosteroids (ICS).