Article
Cell Biology
Vanessa Schimek, Katharina Strasser, Andrea Beer, Samantha Goeber, Natalie Walterskirchen, Christine Brostjan, Catharina Mueller, Thomas Bachleitner-Hofmann, Michael Bergmann, Helmut Dolznig, Rudolf Oehler
Summary: Sporadic apoptosis of tumour cells in colorectal cancer is associated with poor patient prognosis. Apoptotic cancer cells release chemotactic factors that attract neutrophils, inducing changes in neutrophil phenotype and lifespan and promoting interaction with macrophages in the tumor microenvironment, potentially contributing to an immunologically unfavorable environment.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Anderly C. Chueh, Gahana Advani, Momeneh Foroutan, Jai Smith, Nadia Ng, Harshal Nandurkar, Daisy S. Lio, Hong-Jian Zhu, Yuh-Ping Chong, Heather Verkade, Donald J. Fujita, Jeffrey Bjorge, Faiza Basheer, Jet Phey Lim, Ian Luk, Amardeep Dhillon, Anuratha Sakthianandeswaren, Dmitri Mouradov, Oliver Sieber, Frederic Hollande, John M. Mariadason, Heung-Chin Cheng
Summary: The study reveals that downregulation of CHK/MATK expression in CRC cells is associated with promoter hypermethylation, leading to hyperactivation of SFKs and enhanced tumorigenic activity. Restoring CHK/MATK expression reduces SFK activity, inhibiting proliferation and migration of colorectal cancer cells in vitro and in vivo.
Article
Oncology
Fareesa Aftab, Alice Rodriguez-Fuguet, Luis Silva, Ikei S. Kobayashi, Jiao Sun, Katerina Politi, Elena Levantini, Wei Zhang, Susumu S. Kobayashi, Wen Cai Zhang
Summary: AICAR inhibits the growth of EGFR-mutant lung cancer by reducing MUC1 activity and disrupting protein-protein interactions between MUC1-CT and JAK1 and EGFR.
BRITISH JOURNAL OF CANCER
(2023)
Article
Chemistry, Medicinal
Chien-Wei Huang, You-Cian Lin, Chia-Hung Hung, Han-Min Chen, Jiun-Tsai Lin, Chau-Jong Wang, Shao-Hsuan Kao
Summary: Adenine can reduce the invasion potential of DLD-1 cells through the AMPK/integrin/FAK axis, suggesting potential anti-metastatic effects in CRC cells.
Article
Oncology
Liya Liu, Youqin Chen, Xiaoying Lin, Meizhu Wu, Jiapeng Li, Qiurong Xie, Thomas J. Sferra, Yuying Han, Huixin Liu, Liujing Cao, Mengying Yao, Jun Peng, Aling Shen
Summary: SNTB1 is overexpressed in CRC and associated with shorter patient survival. It promotes tumor growth and progression of CRC by reducing PKN2 expression and activating the ERK and AKT signaling pathways.
CANCER CELL INTERNATIONAL
(2021)
Article
Cell Biology
Lei Han, Zeyong Huang, Yan Liu, Lijuan Ye, Dongqi Li, Zhihong Yao, Cao Wang, Ya Zhang, Hang Yang, Zunxian Tan, Jiadai Tang, Zuozhang Yang
Summary: Bone metastasis is a serious complication in lung cancer patients, and microRNAs, such as miR-106a, play important roles in tumour development and metastasis. High expression of miR-106a in lung adenocarcinoma tissues with bone metastasis promotes cell proliferation, migration, and invasion in vitro, as well as bone metastasis in vivo by targeting TP53INP1-mediated pathways. Autophagy partially attenuates the effects of miR-106a on bone metastasis, suggesting potential therapeutic strategies for lung adenocarcinoma.
CELL DEATH & DISEASE
(2021)
Article
Immunology
Mark P. Roberto, Gabriele Varano, Rosa Vinas-Castells, Antony B. Holmes, Rahul Kumar, Laura Pasqualucci, Pedro Farinha, David W. Scott, David Dominguez-Sola
Summary: FOXO1 mutations in B-NHL lead to hyperactivation of PI3K and SAPK/JNK signaling in B cell lines, enhancing cell survival under stressful conditions and providing a competitive advantage. These mutations also disrupt GC homeostasis and predict poor clinical outcomes in human B-NHL cases.
Article
Oncology
Francesca Salvianti, Stefania Gelmini, Irene Mancini, Mario Pazzagli, Serena Pillozzi, Elisa Giommoni, Marco Brugia, Francesco Di Costanzo, Francesca Galardi, Francesca De Luca, Francesca Castiglione, Luca Messerini, Pamela Pinzani, Lorenzo Antonuzzo
Summary: Liquid biopsy, including cfDNA and CTCs, shows prognostic value in KRAS mutated mCRC. CTCs are a negative prognostic marker at baseline, while cfDNA is easily accessible for monitoring tumor mutations over time. In longitudinal monitoring, these two markers complement each other in assessing disease progression.
BRITISH JOURNAL OF CANCER
(2021)
Article
Biotechnology & Applied Microbiology
Zequn Lu, Ning Liu, Huoqing Huang, Yuan Wang, Tao Tu, Xing Qin, Xiaolu Wang, Jie Zhang, Xiaoyun Su, Jian Tian, Yingguo Bai, Huiying Luo, Bin Yao, Honglian Zhang
Summary: Human insulin-like growth factor-1 (IGF-1) and its analog Long R3 IGF-1 (LR3 IGF-1) were successfully recombinantly expressed and produced in the Pichia pastoris expression system by fusion with highly expressed xylanase XynCDBFV. The purified IGF-1 and LR3 IGF-1 displayed excellent bioactivity of cell proliferation compared to the standard IGF-1. Furthermore, high heterologous expression levels of the fusion proteins were achieved by fermentation in a 15-L bioreactor, which could be used for both clinical and scientific applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biology
Yilan Zhen, Carlie L. Cullen, Raphael Ricci, Benjamin S. Summers, Sakina Rehman, Zubair M. Ahmed, Antoinette Y. Foster, Ben Emery, Robert Gasperini, Kaylene M. Young
Summary: Protocadherin 15 regulates ERK1/2 phosphorylation to reduce proliferation and promotes lamellipodial and filopodial dynamics in oligodendrocyte progenitor cells by controlling Cdc42-Arp2/3 activity. It plays a critical role in the function of these cells in the healthy central nervous system and may have implications in glioma progression.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alfonso Garcia-Valverde, Jordi Rosell, Sergi Sayols, David Gomez-Peregrina, Daniel F. Pilco-Janeta, Ivan Olivares-Rivas, Enrique de Alava, Joan Maurel, Jordi Rubio-Casadevall, Anna Esteve, Marta Gut, Claudia Valverde, Jordi Barretina, Joan Carles, George D. Demetri, Jonathan A. Fletcher, Joaquin Arribas, Cesar Serrano
Summary: In most gastrointestinal stromal tumors (GIST), KIT/PDGFRA oncogenic tyrosine kinase signaling is central but GIST cells develop resistance to KIT/PDGFRA suppression. The study found that the E3 ubiquitin ligase Atrogin-1 is critical in GIST adaptive resistance to KIT inhibition, and overexpression of Atrogin-1 enables GIST cells to evade apoptosis through cell quiescence. Targeting both KIT and the ubiquitin pathway may maximize therapeutic response to imatinib treatment.
Article
Oncology
David Sefrioui, Ludivine Beaussire, Andre Gillibert, France Blanchard, Emmanuel Toure, Celine Bazille, Anne Perdrix, Frederic Ziegler, Alice Gangloff, Melanie Hassine, Caroline Elie, Anne-Laure Bignon, Aurelie Parzy, Philippe Gomez, Caroline Thill, Florian Clatot, Jean-Christophe Sabourin, Thierry Frebourg, Jacques Benichou, Karine Bouhier-Leporrier, Marie-Pierre Gallais, Nasrin Sarafan-Vasseur, Pierre Michel, Frederic Di Fiore
Summary: The study confirmed the importance of CEA kinetics in predicting PD, and found that a circulating scoring system combining CEA kinetics, baseline CA19-9, and cfDNA values was independently associated with PD, PFS, and OS in mCRC patients.
BRITISH JOURNAL OF CANCER
(2021)
Editorial Material
Biochemistry & Molecular Biology
Daniel J. Dickinson
Summary: Animal cells establish polarity through the partitioning-defective protein system. The interactions between its four core proteins have been numerous, making it challenging to comprehend the organization and molecular mechanisms involved. In a recent JBC article, the Prehoda group successfully reconstituted some of these interactions in vitro, providing a clearer and simpler understanding of partitioning-defective complex assembly.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Meiling Wang, Jie Liu, Yizeng Tu, Zihan Zhao, Jingjing Qu, Ka Chen, Yonglong Chen, Ying Sun, Hui Zhao, Yi Deng, Chuanyue Wu
Summary: Our study reveals that the focal adhesion protein RSU1 plays a critical role in suppressing ERK signaling during cell-ECM detachment through its interaction with prohibitin 2 (PHB2) in lipid rafts. The binding between RSU1 and PHB2 at specific sites is essential for the activation of ERK signaling in response to ECM adhesion. When cells detach from ECM, RSU1 associates with lipid rafts and interacts with PHB2, ultimately leading to downregulation of ERK signaling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Helen M. Wise, Adam Harris, Nisha Kriplani, Adam Schofield, Helen Caldwell, Mark J. Arends, Ian M. Overton, Nick R. Leslie
Summary: Loss of PTEN function is a common event in aggressive prostate cancers, and PTEN has both lipid phosphatase and protein phosphatase activities. Loss of PTEN protein phosphatase activity leads to lymphocyte infiltration and an increased immune cell gene expression signature.
Article
Oncology
Margaret R. Dunne, James J. Phelan, Adriana J. Michielsen, Aoife A. Maguire, Cara Dunne, Petra Martin, Sinead Noonan, Miriam Tosetto, Robert Geraghty, David Fennelly, Kieran Sheahan, Elizabeth J. Ryan, Jacinth O'Sullivan
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2020)
Article
Oncology
Josephine Hai, Hua Zhang, Jin Zhou, Zhong Wu, Ting Chen, Eleni Papadopoulos, Catriona M. Dowling, Val Pyon, Yuanwang Pan, Jie Bin Liu, Roderick T. Bronson, Heather Silver, Patrick H. Lizotte, Jiehui Deng, Joshua D. Campbell, Lynette M. Sholl, Christine Ng, Ming-Sound Tsao, Cassandra Thakurdin, Adam J. Bass, Kwok-Kin Wong
CLINICAL CANCER RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Kit Chan, Neil Frankish, Tao Zhang, Abdulilah Ece, Aoife Cannon, Jacintha O'Sullivan, Helen Sheridan
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2020)
Article
Immunology
James Joseph Phelan, Kate McQuaid, Colin Kenny, Karl Michael Gogan, Donal J. Cox, Sharee Ann Basdeo, Seonadh O'Leary, Simone Christa Tazoll, Cilian O. Maoldomhnaigh, Mary P. O'Sullivan, Luke A. O'Neill, Maureen J. O'Sullivan, Joseph Keane
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Oncology
Gillian Barber, Akanksha Anand, Katarzyna Oficjalska, James J. Phelan, Aisling B. Heeran, Ewelina Flis, Niamh E. Clarke, Jenny A. Watson, Julia Strangmann, Brian Flood, Hazel O'Neill, Dermot O'Toole, Finbar MacCarthy, Narayanasamy Ravi, John Reynolds, Elaine W. Kay, Michael Quante, Jacintha O'Sullivan, Emma M. Creagh
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2020)
Article
Oncology
Fei Li, Wai-Lung Ng, Troy A. Luster, Hai Hu, Vladislav O. Sviderskiy, Catriona M. Dowling, Kate E. R. Hollinshead, Paula Zouitine, Hua Zhang, Qingyuan Huang, Michela Ranieri, Wei Wang, Zhaoyuan Fang, Ting Chen, Jiehui Deng, Kai Zhao, Hon-Cheong So, Alireza Khodadadi-Jamayran, Mousheng Xu, Angeliki Karatza, Val Pyon, Shuai Li, Yuanwang Pan, Kristen Labbe, Christina Almonte, John T. Poirier, George Miller, Richard Possemato, Jun Qi, Kwok-Kin Wong
Review
Biochemistry & Molecular Biology
Catriona M. Dowling, Hua Zhang, Triona Ni Chonghaile, Kwok-Kin Wong
Summary: Metabolic reprogramming is critical for the survival and growth of non-small cell lung cancer (NSCLC), which exhibits high heterogeneity in their metabolic pathways due to distinct mutations. Understanding the metabolic vulnerabilities of NSCLC cells with specific mutations may lead to the development of novel therapeutic strategies.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Article
Multidisciplinary Sciences
Catriona M. Dowling, Kate E. R. Hollinshead, Alessandra Di Grande, Justin Pritchard, Hua Zhang, Eugene T. Dillon, Kathryn Haley, Eleni Papadopoulos, Anita K. Mehta, Rachel Bleach, Andreas U. Lindner, Brian Mooney, Heiko Dussmann, Darran O'Connor, Jochen H. M. Prehn, Kieran Wynne, Michael Hemann, James E. Bradner, Alec C. Kimmelman, Jennifer L. Guerriero, Gerard Cagney, Kwok-Kin Wong, Anthony G. Letai, Triona Ni Chonghaile
Summary: The study identified a previously unidentified role for HDAC6 in regulating glycolytic metabolism in triple-negative breast cancer. Through a functional genetic approach and state-of-the-art acetylome mass spectrometry, the researchers found that inhibition of HDAC6 reduced glycolytic metabolism both in vitro and in vivo, offering a potential new target for therapeutic interventions in TNBC.
Article
Biochemistry & Molecular Biology
Christina Cahill, Fiona O'Connell, Karl M. Gogan, Donal J. Cox, Sharee A. Basdeo, Jacintha O'Sullivan, Stephen V. Gordon, Joseph Keane, James J. Phelan
Summary: The study found that the iron chelator desferrioxamine, used as an adjunctive treatment to bedaquiline, significantly reduced the bacterial load in human macrophages infected with BCG. Additionally, it increased specific cytokine levels, indicating a potential link between enhanced bactericidal activity and cytokine production with the addition of desferrioxamine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Hai Hu, Alireza Khodadadi-Jamayran, Igor Dolgalev, Hyunwoo Cho, Sana Badri, Luis A. Chiriboga, Briana Zeck, Miguel Lopez De Rodas Gregorio, Catriona M. Dowling, Kristen Labbe, Jiehui Deng, Ting Chen, Hua Zhang, Paul Zappile, Ze Chen, Beatrix Ueberheide, Angeliki Karatza, Han Han, Michela Ranieri, Sittinon Tang, George Jour, Iman Osman, Antje Sucker, Dirk Schadendorf, Aristotelis Tsirigos, Kurt A. Schalper, Vamsidhar Velcheti, Hsin-yi Huang, Yujuan Jin, Hongbin Ji, John T. Poirier, Fei Li, Kwok-Kin Wong
Summary: Significant progress has been made in understanding how tumors evade immune surveillance, with epigenetic modifications playing a critical role in immune invasion. Disruption of genes encoding chromatin modifiers Atf7ip or Setdb1 in tumor cells can restore tumor antigen expression, leading to enhanced tumor immunogenicity and T-cell infiltration. This suggests a potential target for cancer immunotherapy in targeting Atf7ip or Setdb1.
CANCER IMMUNOLOGY RESEARCH
(2021)
Article
Oncology
Noel E. Donlon, Maria Davern, Andrew Sheppard, Robert Power, Fiona O'Connell, Aisling B. Heeran, Ross King, Conall Hayes, Anshul Bhardwaj, James J. Phelan, Margaret R. Dunne, Narayanasamy Ravi, Claire L. Donohoe, Jacintha O'Sullivan, John Reynolds, Joanne Lysaght
Summary: Esophageal cancer patients often present at an advanced stage, with current treatment methods showing limited effectiveness. Understanding the immune characteristics in tumor and lymph node microenvironments can guide future treatment strategies. Nodal metastases play a significant role in prognosis, and the tumor microenvironment exhibits a more immunosuppressive phenotype compared to the lymph node. Blockade of immune checkpoints may offer therapeutic benefits for improving treatment response rates in esophageal adenocarcinoma.
Article
Biochemistry & Molecular Biology
Christina Cahill, Donal J. Cox, Fiona O'Connell, Sharee A. Basdeo, Karl M. Gogan, Cilian o'Maoldomhnaigh, Jacintha O'Sullivan, Joseph Keane, James J. Phelan
Summary: Tuberculosis remains a global health challenge, with patients requiring long, arduous, and complex treatment regimens. Studies show that TB antimicrobials can not only kill bacteria, but also directly affect the immune system. Some TB antimicrobials have been found to significantly alter cellular immunometabolism and mitochondrial function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Martin P. Barr, Anne-Marie Baird, Sophia Halliday, Petra Martin, Emma H. Allott, James Phelan, Greg Korpanty, Linda Coate, Cathal O'Brien, Steven G. Gray, Jane S. Y. Sui, Brian Hayes, Sinead Cuffe, Stephen P. Finn
Summary: Liquid biopsy has the potential to improve patient care in the diagnostic and therapeutic setting in non-small cell lung cancer (NSCLC). It can be used to monitor EGFR mutations and inflammatory markers as prognostic indicators in NSCLC.
Review
Oncology
Robyn Stanley, Saoirse Flanagan, David O'Reilly, Ella Kearney, Jarushka Naidoo, Catriona M. Dowling
Summary: Immunotherapy has revolutionized anti-cancer treatment by developing immune checkpoint inhibitors (ICI) such as CTLA-4 and PD-1. Lung cancer patients benefit from ICIs, leading to increased survival and improved quality of life. However, not all patients respond to immunotherapy and there may be immune-related toxicity.
Article
Oncology
Hua Zhang, Christopher S. Nabel, Dezhi Li, Ruth OConnor, Caroline R. Crosby, Sarah M. Chang, Yuan Hao, Robyn Stanley, Soumyadip Sahu, Daniel S. Levin, Ting Chen, Sittinon Tang, Hsin-Yi Huang, Mary Meynardie, Janaye Stephens, Fiona Sherman, Alison Chafitz, Naoise Costelloe, Daniel A. Rodrigues, Hilda Fogarty, Miranda G. Kiernan, Fiona Cronin, Eleni Papadopoulos, Magdalena Ploszaj, Vajira Weerasekara, Jiehui Deng, Patrick Kiely, Nabeel Bardeesy, Matthew G. Vander Heiden, Triona Ni Chonghaile, Catriona M. Dowling, Kwok-Kin Wong
Summary: In KRAS-mutant NSCLC, co-occurring alterations in LKB1 confer a negative prognosis. Our recent work on HDAC6 inhibition revealed impaired activity of enzymes involved in glycolysis. Therefore, we explored the therapeutic window for HDAC6 inhibition in metabolically-active KRAS-mutant lung tumors.
JOURNAL OF THORACIC ONCOLOGY
(2023)
