Article
Oncology
Hale Guler Kara, Neslihan Pinar Ozates, Aycan Asik, Cumhur Gunduz
Summary: This study evaluates the antineoplastic effects of Nanog inhibition using Amcasertib in ovarian cancer cells. The results show that Amcasertib effectively inhibits cell proliferation and induces apoptosis, while also impeding cell invasion and migration. It demonstrates high anticancer efficacy, particularly in ER-negative ovarian cancer and cancer stem cells.
Article
Oncology
Parisa Vasefifar, Souzan Najafi, Rouhollah Motafakkerazad, Mohammad Amini, Sahar Safaei, Basira Najafzadeh, Hajar Alemohammad, Mahdi Jafarlou, Behzad Baradaran
Summary: Regardless of advances in cancer treatment, gastric cancer incidence is increasing globally. Nanog, as a transcription factor involved in stemness, plays a crucial role in tumorigenesis, metastasis, and chemosensitivity. This study evaluated the effects of Nanog suppression on cisplatin chemosensitivity and tumorigenesis in gastric cancer cells. The findings showed that Nanog overexpression correlated with poor survival in gastric cancer patients, while Nanog silencing increased cell sensitivity to cisplatin through apoptosis induction, inhibited cell migration, and reduced CD44 and SOX-2 expression. Overall, Nanog may be a promising target for improving gastric cancer outcomes with cisplatin-based therapies.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kyung-Taek Kang, Min-Joo Shin, Hye-Ji Moon, Kyung-Un Choi, Dong-Soo Suh, Jae-Ho Kim
Summary: This study found that TRRAP overexpression increases NANOG protein stability and enhances the tumorigenic potential of colon cancer stem cells. TRRAP inhibits the ubiquitination degradation of NANOG by binding to it. TRRAP knockdown decreases the expression of CD44, a cancer stem cell marker, and increases the expression of P53, a tumor suppressor gene, in colon cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Laura M. Chambers, Emily L. Esakov Rhoades, Rashmi Bharti, Chad Braley, Surabhi Tewari, Lexie Trestan, Zahraa Alali, Defne Bayik, Justin D. Lathia, Naseer Sangwan, Peter Bazeley, Amy S. Joehlin-Price, Zeneng Wang, Sumita Dutta, Mohammed Dwidar, Adeline Hajjar, Philip P. Ahern, Jan Claesen, Peter Rose, Roberto Vargas, J. Mark Brown, Chad M. Michener, Ofer Reizes
Summary: This study investigated the impact of antibiotic treatment on the growth and cisplatin sensitivity of ovarian cancer. The findings suggest that antibiotic treatment may promote tumor growth and suppress cisplatin sensitivity. Antibiotic treatment led to reduced apoptosis, increased DNA damage repair, enhanced angiogenesis, and increased frequency of cisplatin-augmented cancer stem cells.
Article
Biochemistry & Molecular Biology
Young Joo Lee, Sung Wook Kim, Min Hyung Jung, Young Sun Kim, Kyung Sook Kim, Dong Soo Suh, Ki Hyung Kim, Eun Ha Choi, Jongmin Kim, Byung Su Kwon
Summary: Ovarian cancer stem-like cells (CSCs) are sensitive to plasma-activated medium (PAM) treatment, which inhibits CSC traits and exhibits synergistic cytotoxicity with cisplatin (CDDP). This study suggests that PAM could be a promising intraperitoneal chemotherapy for ovarian cancer, enhancing antitumor efficacy and reducing side effects.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Genetics & Heredity
Parisa Vasefifar, Rouhollah Motafakkerazad, Leili Aghebati Maleki, Souzan Najafi, Farid Ghrobaninezhad, Basira Najafzadeh, Hajar Alemohammad, Mohammad Amini, Amir Baghbanzadeh, Behzad Baradaran
Summary: Cancer stem cells (CSCs) play a crucial role in tumor initiation and development, as well as in chemoresistance and metastasis. Nanog, a cell surface marker, is upregulated in various cancers and contributes to the stemness, self-renewal, metastasis, invasiveness, and chemoresistance of cancer cells. The function of Nanog in multiple cancers is of significant importance for cancer treatment.
Article
Biochemistry & Molecular Biology
Liang Hao, Jia-Mei Wang, Bao-Qin Liu, Jing Yan, Chao Li, Jing-Yi Jiang, Fu-Ying Zhao, Huai-Yu Qiao, Hua-Qin Wang
Summary: TRIM29 expression is upregulated in cisplatin-resistant ovarian cancer cells and clinical tissues, and is associated with poor prognosis. TRIM29 enhances the CSC-like characteristics of ovarian cancer cells, while knockdown of YTHDF1 suppresses these traits. The study suggests TRIM29 and YTHDF1 may serve as potential therapeutic targets for combating ovarian cancer recurrence.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Medicine, Research & Experimental
Hui Xu, Fengshu Zhao, Di Wu, Yunxia Zhang, Xueyang Bao, Fangfang Shi, Yunlang Cai, Jun Dou
Summary: The immune effects of cancer stem cells (CSCs) vaccines on ovarian cancer (OC) were evaluated. The CSC vaccines were capable of significantly provoking immune responses to autologous tumor antigens in vaccinated mice, resulting in inhibited tumor growth, prolonged survival, and decreased CSC counts in OC tissues. The antitumor efficacy of the vaccines was reduced when the mucin-1 expression in CSC vaccines was down-regulated.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Cell Biology
Puja Agrawal, Sridhar Rao
Summary: Cell fate decisions rely on gene expression changes mediated by regulatory elements like enhancers and CTCF binding sites. These elements play crucial roles in early mammalian development and impact cell-type specific gene expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Yue Wu, Ting Wang, Lin Xia, Mei Zhang
Summary: The study identified the significant role of long non-coding RNA WDFY3-AS2 in ovarian cancer cells, showing its impact on cisplatin resistance by regulating the expression of miR-139-5p/SDC4. Additionally, WDFY3-AS2 was found to have a promoting effect on cell proliferation, migration, and invasion.
CANCER CELL INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Xueqin Sun, Xinghua Diao, Xiaolin Zhu, Xuexue Yin, Guangying Cheng
Summary: The study identified that high expression of MBNL3 in ovarian cancer patients is positively correlated with paclitaxel resistance and poor prognosis, as it increases cancer cell viability and decreases apoptosis in ovarian stem-like cells through upregulating Nanog gene expression, suggesting that the MBNL3-Nanog axis could be a potential therapeutic target for managing paclitaxel resistance in ovarian cancer.
JOURNAL OF BIOCHEMISTRY
(2021)
Article
Cell Biology
Cristina Corno, Padraig D'Arcy, Marina Bagnoli, Biagio Paolini, Matteo Costantino, Nives Carenini, Elisabetta Corna, Paola Alberti, Delia Mezzanzanica, Diego Colombo, Stig Linder, Noemi Arrighetti, Paola Perego
Summary: This study identified the increased activity of ubiquitin protease USP8 in cisplatin-resistant cells and demonstrated that silencing of USP8 decreased cancer cell survival and increased sensitivity to cisplatin. Immunohistochemical analysis of clinical specimens indicated that USP8 positivity is associated with adverse prognosis in advanced stage ovarian carcinoma. Therefore, USP8 may serve as a diagnostic marker and therapeutic target to improve the efficacy of platinum-based therapy in ovarian carcinoma.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pathology
Basira Najafzadeh, Rouhollah Motafakkerazad, Souzan Najafi, Mohammad Amini, Hajar Alemohammad, Parisa Vasefifar, Behzad Baradaran
Summary: This study aimed to evaluate the effect of Nanog suppression on the chemosensitivity of lung cancer cells to Cisplatin. The results showed that silencing Nanog significantly increased the sensitivity of lung cancer cells to Cisplatin through apoptosis induction. Additionally, Nanog suppression prevented colony formation and migration abilities of the cancer cells. These findings suggest that silencing Nanog combined with Cisplatin could be a promising treatment approach for lung cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Cell Biology
Qi Zhang, Jiamei Wang, Huaiyu Qiao, Lingyue Huyan, Baoqin Liu, Chao Li, Jingyi Jiang, Fuying Zhao, Huaqin Wang, Jing Yan
Summary: The study suggests that ISG15 may have context-dependent effects on tumor progression, with ISG15 being decreased in cisplatin-resistant ovarian cancer cells. Ectopic expression of ISG15 was found to inhibit CSC-like phenotypes and tumor formation, while downregulation of ISG15 promoted CSC-like features in cisplatin-sensitive ovarian cancer cells. Low ISG15 expression was associated with poor prognosis in ovarian cancer patients, indicating a potential therapeutic target.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Review
Cell Biology
Basira Najafzadeh, Zahra Asadzadeh, Rohollah Motafakker Azad, Ahad Mokhtarzadeh, Amir Baghbanzadeh, Hajar Alemohammad, Mahdi Abdoli Shadbad, Parisa Vasefifar, Souzan Najafi, Behzad Baradaran
Summary: Cancer stem cells (CSCs) are a unique population in tumors, and the transcription factor NANOG plays a pivotal role in conferring CSCs with their characteristics and functions. Understanding and targeting NANOG may be key in diminishing CSCs, sensitizing tumors to chemotherapy, and eradicating cancer cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Oncology
Fan Zhang, Lihong Lou, Bo Peng, Xiaotian Song, Ofer Reizes, Alexandru Almasan, Zihua Gong
Article
Multidisciplinary Sciences
Laura M. Chambers, Emily Esakov, Chad Braley, Mariam AlHilli, Chad Michener, Ofer Reizes
Article
Multidisciplinary Sciences
Matthew Knarr, Rita A. Avelar, Sreeja C. Sekhar, Lily J. Kwiatkowski, Michele L. Dziubinski, Jessica McAnulty, Stephanie Skala, Stefanie Avril, Ronny Drapkin, Analisa DiFeo
NATURE COMMUNICATIONS
(2020)
Review
Oncology
Max Horowitz, Emily Esakov, Peter Rose, Ofer Reizes
ANNALS OF TRANSLATIONAL MEDICINE
(2020)
Article
Oncology
Laura M. Chambers, Michelle Kuznicki, Meng Yao, Anna Chichura, Morgan Gruner, Ofer Reizes, Robert Debernardo, Peter G. Rose, Chad Michener, Roberto Vargas
GYNECOLOGIC ONCOLOGY
(2020)
Meeting Abstract
Oncology
M. P. Horowitz, E. L. Esakov, C. Hong, C. M. Michener, P. G. Rose, T. H. Hwang, R. DeBernardo, O. Reizes
GYNECOLOGIC ONCOLOGY
(2020)
Article
Oncology
Laura M. Chambers, Chad M. Michener, Peter G. Rose, Ofer Reizes, Meng Yao, Roberto Vargas
Summary: This retrospective cohort study aimed to investigate the impact of antibiotics (ABX) on immunotherapy (ICI) response rate (RR), progression-free survival (PFS), and overall survival (OS) in women with recurrent endometrial (EC), cervical (CC) and ovarian cancer (OC). The results showed that the use of antibiotics had different effects on ICI RR, PFS, and OS in these women, with some ABX significantly reducing RR, PFS, and OS.
GYNECOLOGIC ONCOLOGY
(2021)
Article
Oncology
Anil Belur Nagaraj, Matthew Knarr, Sreeja Sekhar, R. Shae Connor, Peronne Joseph, Olga Kovalenko, Alexis Fleming, Arshia Surti, Elmar Nurmemmedov, Luca Beltrame, Sergio Marchini, Michael Kahn, Analisa DiFeo
Summary: Wnt signaling plays a critical role in ovarian cancer, and miR-181a has been identified as a novel driver of the Wnt/β-catenin signaling pathway, promoting stemness and chemoresistance. Targeting the miR-181a-SFRP4 axis may offer a potential therapeutic strategy for HGSOC.
Correction
Biochemistry & Molecular Biology
Haihua Feng, Jingchen Lu, Xiaotian Song, Angkana Thongkum, Fan Zhang, Lihong Lou, Ofer Reizes, Alexandru Almasan, Zihua Gong
NUCLEIC ACIDS RESEARCH
(2021)
Article
Reproductive Biology
Katie K. Crean-Tate, Chad Braley, Goutam Dey, Emily Esakov, Caner Saygin, Alexandria Trestan, Daniel J. Silver, Soumya M. Turaga, Elizabeth V. Connor, Robert DeBernardo, Chad M. Michener, Peter G. Rose, Justin Lathia, Ofer Reizes
Summary: Therapeutic targeting of cisplatin resistant endometrioid ovarian cancer through LCKi treatment followed by LCKi-cisplatin combination therapy leads to chemosensitization to platinum chemotherapy, demonstrating increased cell viability and apoptosis, and slowed tumor growth in vivo.
JOURNAL OF OVARIAN RESEARCH
(2021)
Review
Oncology
Laura M. Chambers, Parker Bussies, Roberto Vargas, Emily Esakov, Surabhi Tewari, Ofer Reizes, Chad Michener
Summary: The relationship between the microbiota of the gastrointestinal and female reproductive tracts and gynecologic cancer is being studied, with alterations in bacterial populations potentially favoring carcinogenesis. Specific bacteria have been linked to increased risk for uterine, ovarian, and cervical cancers.
CURRENT ONCOLOGY REPORTS
(2021)
Article
Pathology
Jutta Huvila, Dawn R. Cochrane, Monica Ta, Christine Chow, Kendall Greening, Samuel Leung, Anthony N. Karnezis, Analisa DiFeo, David G. Huntsman
Summary: Ovarian carcinoma histotypes vary in STING expression, possibly reflecting pathway activation or tissue origin. Further research is needed to determine if the STING signaling pathway is active and if these tumors are suitable for therapeutic interventions that trigger innate immune activation.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2021)
Article
Oncology
Richard T. Lee, Peiying Yang, Asrar Alahmadi, Jennifer McQuade, Eric Yuan, Analisa Difeo, Goutham Narla, Ahmed Kaseb
Summary: This prospective observational case series study on advanced HCC patients treated with mistletoe extract VF-2 suggests potential anticancer activity against HCC. Some patients showed significant AFP reduction and one patient had stable disease for 9 months. Further research is needed to identify the active compound and predictive markers of response.
CASE REPORTS IN ONCOLOGY
(2021)
Meeting Abstract
Oncology
Laura Chambers, Emily Esakov, Chad Braley, Leila Edelman, Roberto Vargas, Justin Lathia, Chad Michener, Ofer Reizes
CLINICAL CANCER RESEARCH
(2020)
Meeting Abstract
Obstetrics & Gynecology
Elliott G. Richards, Jenna M. Rehmer, Emily Esakov, Amy Joehlin-Price, Andres Chiesa-Vottero, Chad Braley, Zahraa A. Alali, Jeffrey Goldberg, Ofer Reizes
REPRODUCTIVE SCIENCES
(2020)