Article
Oncology
Fatima Junaid, Goran Tomic, Richard Kemp, Doug J. Winton
Summary: Using human colon cancer DLD-1 cells, the study engineered a cell line with inducible Snail expression and found that partial EMT induced by Snail overexpression in DLD-1 cells leads to chemoresistance, increased tumor growth, and decreased apoptosis, highlighting the importance of bioavailable Snail in determining the phenotypic outcome.
Article
Oncology
Ana Pavlic, Emanuela Bostjancic, Rajko Kavalar, Bojan Ilijevec, Serena Bonin, Fabrizio Zanconati, Nina Zidar
Summary: This study investigates the involvement of epithelial-mesenchymal transition (EMT) in tumor budding (TB) and poorly differentiated clusters (PDCs) in colorectal cancer (CRC). The results suggest that both TB and PDCs are associated with partial EMT, but they exhibit discrete differences in morphology and expression of EMT-related markers.
JOURNAL OF PATHOLOGY
(2022)
Article
Environmental Sciences
Rita Bonfiglio, Renata Sisto, Stefano Casciardi, Valeria Palumbo, Maria Paola Scioli, Erica Giacobbi, Francesca Servadei, Gerry Melino, Alessandro Mauriello, Manuel Scimeca
Summary: This study investigated the presence of aluminum in human colon cancer samples and its potential association with biological processes involved in cancer progression. Aluminum was found in tumor areas of 24% of patients and was associated with epithelial to mesenchymal transition (EMT) and cell death. Additional analyses revealed higher tumor mutational burden and mutations in genes related to EMT and apoptosis in aluminum-positive colon cancers. Understanding the molecular mechanisms of aluminum toxicity may improve strategies for the management of colon cancer patients.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Biochemistry & Molecular Biology
Yeon-Jee Kahm, Uhee Jung, Rae-Kwon Kim
Summary: CTNNAL1 is a protein involved in cell-cell adhesion and has been found to have various roles in cancer development and progression. It acts as a cancer suppressor gene in breast cancer, but as a cancer driver gene in melanoma and lung cancer. This study reveals that CTNNAL1 regulates cancer stem cells (CSCs) in lung cancer and glioblastoma, affecting their ability to migrate, invade, and resist treatments like irradiation. Furthermore, CTNNAL1 is also involved in the secretion of CCL2, a chemokine known to influence malignancy and metastasis in cancer. These findings suggest that CTNNAL1 may serve as a potential target for treating lung CSCs and glioma stem cells, as well as a marker of malignancy.
Article
Biochemistry & Molecular Biology
Long Xu, Jian Wang, Buhan Liu, Jiaying Fu, Yuanxin Zhao, Sihang Yu, Luyan Shen, Xiaoyu Yan, Jing Su
Summary: Epithelial ovarian cancer has the highest mortality rate among gynecological malignant tumors, largely due to metastasis. This study found that HDAC9 plays different roles in different histological subtypes of ovarian cancer and may be a potential target for personalized treatment.
Article
Biotechnology & Applied Microbiology
Minghan Guan, Yifeng Huang, Xiaowen Lin
Summary: This study found that sufentanil can inhibit the proliferation, migration, invasion, and epithelial-mesenchymal transition of lung cancer cells by regulating the Wnt/beta-catenin signaling pathway.
Article
Oncology
Antonia M. Stehr, Guangxia Wang, Richard Demmler, Marc P. Stemmler, Julia Krug, Philipp Tripal, Benjamin Schmid, Carol Geppert, Arndt Hartmann, Luis E. Munoz, Janina Schoen, Simon Voelkl, Susanne Merkel, Christoph Becker, Georg Schett, Robert Gruetzmann, Elisabeth Naschberger, Martin Herrmann, Michael Stuerzl
Summary: Neutrophil extracellular traps (NETs), released by neutrophils, have been found in colorectal cancer (CRC) tissues and are associated with disease progression. This study used high-resolution microscopy to detect citrullinated NETs in colon cancer tissues and found that citrullinated NETs were significantly associated with high tumour grades and lymph node metastasis. In vitro experiments also showed that purified NETs induced filopodia formation and cell motility in CRC cell lines, indicating their potential contribution to CRC metastasis.
JOURNAL OF PATHOLOGY
(2022)
Article
Medicine, Research & Experimental
Chi-Chiu Lee, Chia-Jui Yu, Sushree Shankar Panda, Kai-Chi Chen, Kang-Hao Liang, Wan-Chen Huang, Yu-Shiuan Wang, Pei-Chin Ho, Han-Chung Wu
Summary: This study explores the interaction between EpCAM and HGFR and its impact on the metastatic and invasive abilities of colon cancer cells. The results demonstrate that EpCAM binds to HGFR and activates the ERK and FAK-AKT signaling pathways, leading to the promotion of colon cancer metastasis. Furthermore, the combined treatment with EpCAM and HGFR antibodies significantly inhibits tumor progression and improves patient survival.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Liang Zhang, Chuan-fu Ren, Zhi Yang, Long-bo Gong, Chao Wang, Min Feng, Wen-xian Guan
Summary: FOXS1 plays a role in promoting cell proliferation, migration, invasion, and metastasis in colorectal cancer through the Wnt/beta-catenin signaling pathway, making it a potential target for CRC treatment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Health Care Sciences & Services
Constantin Busuioc, Cristina Alexandra Ciocan-Cartita, Cornelia Braicu, Oana Zanoaga, Lajos Raduly, Monica Trif, Mihai-Stefan Muresan, Calin Ionescu, Cristina Stefan, Carmen Crivii, Nadim Al Hajjar, Simona Margarit, Ioana Berindan-Neagoe
Summary: The study utilized bioinformatics approaches to investigate alterations in EMT-related genes in colon adenocarcinoma patients, identifying key gene changes related to prognosis in COAD. These findings may help develop biomarkers for recurrence prediction in COAD patients and improve risk stratification for patients.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Oncology
Chunhua Hu, Mujie Ye, Jianan Bai, Pengfei Liu, Feiyu Lu, Jinhao Chen, Ping Yu, Tiaotiao Chen, Xiaoting Shi, Qiyun Tang
Summary: This study investigated the role of upregulated metabolites of aged serum in colorectal cancer (CRC) progression. Methylmalonic acid (MMA) was found to be responsible for tumorigenesis and metastasis in CRC. Through Wnt/beta-catenin signaling pathway mediated epithelial-mesenchymal transition (EMT), MMA promoted CRC progression. These findings highlight the importance of age-related metabolic reprogramming in CRC and suggest a potential therapeutic target for elderly patients.
CANCER CELL INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Young Yun Jung, Chakrabhavi Dhananjaya Mohan, Huiyan Eng, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Kanchugarakoppal S. Rangappa, Gautam Sethi, Alan Prem Kumar, Kwang Seok Ahn
Summary: This study demonstrates that 2,3,5,6-Tetramethylpyrazine (TMP) can suppress the EMT program in colon cancer cells by regulating MnSOD and related proteins, and inhibit the proliferation, migration, and invasion of tumor cells.
Correction
Oncology
Juntao Sun, Tianyu Zhang, Mengmeng Cheng, Liwen Hong, Chen Zhang, Mengfan Xie, Peijun Sun, Rong Fan, Zhengting Wang, Lei Wang, Jie Zhong
Summary: An amendment to the paper has been published and is accessible through the original article.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Yanlong Shi, Chang Ge, Debao Fang, Wei Wei, Li Li, Qian Wei, Hongzhu Yu
Summary: NCAPG is over-expressed in colorectal cancer and is associated with clinicopathological characteristics. Silencing NCAPG can suppress the proliferation, migration, and invasion of CRC cells, while overexpression of NCAPG promotes these abilities. Mechanistically, NCAPG activates the Wnt/beta-catenin signaling pathway to facilitate CRC invasion and metastasis.
CANCER CELL INTERNATIONAL
(2022)
Review
Oncology
Xiaobo Zheng, Fuzhen Dai, Lei Feng, Hong Zou, Li Feng, Mingqing Xu
Summary: The interplay between epithelial-mesenchymal plasticity and cancer stem cells plays a crucial role in cancer progression, affecting the aggressiveness of tumor cells and their response to treatment. Understanding the dynamic nature of EMT as a hybrid state rather than a binary model is important for unraveling the complexities of cancer biology and developing targeted therapeutic interventions.
FRONTIERS IN ONCOLOGY
(2021)
Editorial Material
Pharmacology & Pharmacy
Maria Teresa Esposito, Anna Hagstroem-Andersson, Ronald W. Stam, Stefania Bortoluzzi
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Stefania Fiorcari, Claudio Giacinto Atene, Rossana Maffei, Nicolo Mesini, Giulia Debbia, Corrado Colasante, Stefano Pozzi, Emiliano Barbieri, Monica Maccaferri, Giovanna Leonardi, Leonardo Potenza, Mario Luppi, Roberto Marasca
Summary: Patients with chronic lymphocytic leukemia (CLL) have a reduced immune response to the SARS-CoV-2 vaccine, particularly those under treatment with ibrutinib. Therefore, it is necessary to optimize the vaccination strategy and strengthen preventive measures against COVID-19 in CLL patients.
HEMATOLOGICAL ONCOLOGY
(2023)
Article
Biochemical Research Methods
Alessia Buratin, Stefania Bortoluzzi, Enrico Gaffo
Summary: Circular RNAs (circRNAs) are closed transcripts involved in regulatory pathways and disease mechanisms. Standard differential expression assessment methods may not perform well on circRNA expression data. We compared different pipelines and found that Limma-Voom had the most consistent performance, while some single-cell RNA-seq (scRNA-seq) methods also performed well. Increasing the number of replicates improved the performance of all methods. Careful design and choice of differential expression analysis methods are important for circRNA expression studies.
BRIEFINGS IN BIOINFORMATICS
(2023)
Review
Oncology
Nicolo Mesini, Stefania Fiorcari, Claudio Giacinto Atene, Rossana Maffei, Leonardo Potenza, Mario Luppi, Roberto Marasca
Summary: In recent decades, the Notch pathway has been recognized as an important regulatory signaling in cell-fate decisions and its alteration has been linked to tumor genesis and progression. The focus of this review is on the activity of Notch2 receptor, which has been found to be mutated in CLL, SMZL, and NMZL. A better understanding of the pathogenic role of Notch2 and the development of targeted therapies are desired.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Alessia Buratin, Cristina Borin, Caterina Tretti Parenzan, Anna Dal Molin, Silvia Orsi, Andrea Binatti, Katharina Simon, Maddalena Paganin, Valentina Serafin, Enrico Gaffo, Geertruij te Kronnie, Pieter Van Vlierberghe, Silvia Bresolin, Stefania Bortoluzzi
Summary: Circular RNAs (circRNAs) are recently discovered to have a significant impact on leukemogenic mechanisms, particularly in T-cell Acute Lymphoblastic Leukemia (T-ALL). This study focuses on a specific circRNA called circFBXW7, which is highly expressed in T-cells and plays a role in tumor suppression. The depletion of circFBXW7 leads to increased leukemic cell viability, activation of pro-proliferative pathways, and elevated levels of MYC and intracellular NOTCH1 proteins. These findings suggest that circFBXW7 acts as a suppressor in T-ALL and enhances the aggressive phenotype of the disease.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Claudia Sorbi, Silvia Belluti, Claudio Giacinto Atene, Federica Marocchi, Pasquale Linciano, Neena Roy, Elia Paradiso, Livio Casarini, Simone Ronsisvalle, Tommaso Zanocco-Marani, Livio Brasili, Luisa Lanfrancone, Carol Imbriano, Giulia Di Rocco, Silvia Franchini
Summary: The management of advanced-stage melanoma is challenging due to resistance to current therapies. Therefore, developing alternative strategies is important. Recent research has identified a potent S2R modulator (BS148) that effectively targets melanoma. Mechanistic studies revealed that BS148 activates the endoplasmic reticulum stress response and downregulates genes related to the cholesterol pathway and activates the MAPK signaling pathway. Furthermore, BS148 treatment reduces melanoma cell viability and migration in patient-derived xenograft (PDX) cells, demonstrating its potential as a promising therapeutic target for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Angela Lauriola, Pierpaola Davalli, Gaetano Marverti, Spartaco Santi, Andrea Caporali, Domenico D'Arca
Summary: Immunotherapy uses the body's immune system to fight against cancer, and it has greatly improved patient outcomes for several types of tumors. However, many patients have not yet benefited from this treatment. Combination strategies targeting different cellular pathways are predicted to play a key role in cancer immunotherapy. This review discusses the effects of tumor cell death and immune system engagement on oxidative stress and ubiquitin ligase pathways, as well as combinations of immunotherapies and immunomodulatory targets. The importance of imaging techniques for monitoring tumor responses and side effects is also highlighted.
Letter
Oncology
Damanti Carlotta Caterina, Ferrone Lavinia, Gaffo Enrico, Garbin Anna, Tosato Anna, Contarini Giorgia, Gallingani Ilaria, Angioni Roberta, Molon Barbara, Borile Giulia, Carraro Elisa, Pillon Marta, Scarmozzino Federico, Dei Tos Angelo Paolo, Pizzi Marco, Ciscato Francesco, Rasola Andrea, Biffi Alessandra, Bortoluzzi Stefania, Lovisa Federica, Mussolin Lara
CANCER COMMUNICATIONS
(2023)
Article
Biology
Roberta Lotti, Claudio Giacinto Atene, Emma Dorotea Zanfi, Matteo Bertesi, Carlo Pincelli, Tommaso Zanocco-Marani
Summary: Researchers have developed new active models to mimic the main forms of Pemphigus, including PV and mucocutaneous Pemphigus. Immunized animals and animals that received splenocytes from the immunized donors produced high concentrations of circulating antibodies against specific antigens, but the effectiveness of treatment with corticosteroids was limited.
Review
Biochemistry & Molecular Biology
Diana Luna Buitrago, Ruth C. Lovering, Andrea Caporali
Summary: MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression at the post-transcriptional level. They play critical roles in various biological and pathological processes. The field of miRNA research has advanced significantly, leading to the development of numerous online tools for investigating miRNA function and their impact on essential cellular processes. This review provides an overview of available resources for studying miRNAs, including their sequences, target prediction/validation, disease associations, pathway analysis, and genetic variants.
Article
Pharmacology & Pharmacy
Anna Garbin, Giorgia Contarini, Carlotta C. Damanti, Anna Tosato, Stefania Bortoluzzi, Enrico Gaffo, Marco Pizzi, Elisa Carraro, Luca Lo Nigro, Luciana Vinti, Marta Pillon, Alessandra Biffi, Federica Lovisa, Lara Mussolin
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Biochemical Research Methods
Marieke Vromman, Jasper Anckaert, Stefania Bortoluzzi, Alessia Buratin, Chia-Ying Chen, Qinjie Chu, Trees-Juen Chuang, Roozbeh Dehghannasiri, Christoph Dieterich, Xin Dong, Paul Flicek, Enrico Gaffo, Wanjun Gu, Chunjiang He, Steve Hoffmann, Osagie Izuogu, Michael S. Jackson, Tobias Jakobi, Eric C. Lai, Justine Nuytens, Julia Salzman, Mauro Santibanez-Koref, Peter Stadler, Olivier Thas, Eveline Vanden Eynde, Kimberly Verniers, Guoxia Wen, Jakub Westholm, Li Yang, Chu-Yu Ye, Nurten Yigit, Guo-Hua Yuan, Jinyang Zhang, Fangqing Zhao, Jo Vandesompele, Pieter-Jan Volders
Summary: This study evaluates and validates computational tools for detecting circRNAs, finding most tools to be highly precise with variations in sensitivity and total detection.
Article
Physiology
Tijana Mitic, Andrea Caporali
Summary: This article discusses the role and mechanisms of non-coding RNAs in endothelial cells, as well as their involvement in cell-to-cell communication and transcriptional regulation.
CURRENT OPINION IN PHYSIOLOGY
(2023)
Review
Medicine, Research & Experimental
Rossana Maffei, Stefania Fiorcari, Claudio Giacinto Atene, Silvia Martinelli, Nicolo Mesini, Flora Pilato, Ivana Lagreca, Patrizia Barozzi, Giovanni Riva, Vincenzo Nasillo, Ambra Paolini, Fabio Forghieri, Leonardo Potenza, Tommaso Trenti, Enrico Tagliafico, Mario Luppi, Roberto Marasca
Summary: Interferon regulatory factor 4 (IRF4) plays a crucial role in regulating B cell development and is associated with B cell malignancies. The complexity of IRF4 functions includes its involvement in different DNA-binding affinity, target motifs, and transcriptional partners.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
