Article
Biotechnology & Applied Microbiology
Zhenshang Xu, Rongling Zhang, Ting Wang, Jian Kong
Summary: The study identified the key role of the N-terminus of L. amylovorus FaeLam in its secretion by L. plantarum and E. coli, offering a new method for protein secretion. This is the first reported protein that can be secreted out of both Gram-positive and Gram-negative bacterial cells.
MICROBIAL CELL FACTORIES
(2021)
Article
Agricultural Engineering
Shujun Liu, Lubna Soomro, Xue Wei, Xufeng Yuan, Tianyi Gu, Zhen Li, Yulu Wang, Yuming Bao, Fengzhong Wang, Boting Wen, Fengjiao Xin
Summary: By enhancing the structure of feruloyl esterase and selecting more efficient mutants, this study successfully increased the yield of ferulic acid, providing a new direction for industrial production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Dan Shen, Shaotong Ma, Xiangfei Li, Yingjian Lu
Summary: This study found that supplementation with a Lactobacillus species producing feruloyl esterase (FAEb) can alleviate ulcerative colitis in mice by reducing body weight loss, colon length shortening, fecal occult blood severity, and disease activity index. FAEb supplementation also improves antioxidant capacity, promotes beneficial short-chain fatty acid production, increases expression of tight junction proteins, reduces histopathological scores, and prevents mucous barrier damage in the gut. Additionally, FAEb supplementation inhibits inflammatory signaling pathways, increases beneficial bacteria abundance, and regulates gut microbiota balance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Shang Lin, Cameron J. Hunt, Jesper Holck, Jesper Brask, Kristian B. R. M. Krogh, Anne S. Meyer, Casper Wilkens, Jane W. Agger
Summary: The study found that esterases from various bacteria and fungi can catalyze the release of diferulic acids from complex arabinoxylans. By comparing the activity of the enzymes on synthetic substrates and diFA release, a detailed structure-function analysis is needed to explain the diFA releasing ability of the enzymes. The findings contribute to a better understanding of the role of esterases in the degradation of complex arabinoxylans and provide new opportunities for enzyme-assisted upgrading of complex bran arabinoxylans.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Sangeun Jeon, Jisub Hwang, Hackwon Do, Ly Thi Huong Luu Le, Chang Woo Lee, Wanki Yoo, Min Ju Lee, Seung Chul Shin, Kyeong Kyu Kim, Han-Woo Kim, Jun Hyuck Lee
Summary: In this study, a feruloyl esterase (LaFae) from Lactobacillus acidophilus was obtained and characterized. LaFae could effectively produce ferulic acid from wheat bran, rice bran, and corn stalks. Xylanase supplementation was found to enhance LaFae activity, increasing ferulic acid production. The crystal structures of LaFae revealed the active site configuration and the role of a gatekeeper residue in substrate binding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Haowen Zhang, Boting Wen, Yusi Liu, Guoming Du, Xue Wei, Khandaker Md Sharif Uddin Imam, Huan Zhou, Shilong Fan, Fengzhong Wang, Yulu Wang, Fengjiao Xin
Summary: This study revealed the high activity substrate and crystal structure of LP_0796, demonstrating the importance of certain key residues to its catalytic activity, while also identifying mutation sites that can enhance catalytic efficiency.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Liya Mei, Ying Chen, Jialiang Wang, Jian Lu, Jianxin Zhao, Hao Zhang, Gang Wang, Wei Chen
Summary: This study aimed to explore the effect of different strains of Lactobacillus fermentum on IgA secretion from intestinal mucosa, and found that different strains have different stimulating effects. Additionally, it was speculated that different strains may stimulate IgA production through non-inflammatory pathways.
Article
Biochemistry & Molecular Biology
Guoming Du, Yulu Wang, Yuebin Zhang, Haiyan Yu, Shujun Liu, Xiaochen Ma, Hao Cao, Xue Wei, Boting Wen, Zhen Li, Shilong Fan, Huan Zhou, Fengjiao Xin
Summary: This study identified a novel cold-adapted feruloyl esterase and revealed the connection between oligomerization and the enzyme's catalytic function. Disrupting the enzyme interface led to reduced catalytic activity and stability, as well as a decrease in the release of ferulic acid. These findings are important for improving the industrial application of the enzyme.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Fang Li, Hui Huang, Yangkun Wu, Zhe Lu, Xianrong Zhou, Fang Tan, Xin Zhao
Summary: Research has shown that Lactobacillus fermentum HFY06 can effectively alleviate age-related symptoms in elderly mice by activating the Nrf2 signaling pathway and increasing levels of antioxidant enzymes. This suggests that consumption of HFY06 may help prevent aging and reduce oxidative stress.
Article
Biochemistry & Molecular Biology
Yan Zhao, Leilei Yu, Fengwei Tian, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai
Summary: Research has shown the presence of Lactobacillus fermentum in the human gut and the potential health benefits it offers. A new optimized culture medium called LFMATA has been developed to isolate L. fermentum strains from human fecal samples more efficiently, leading to a 10-fold increase in the isolation rate compared to the previous medium LAMVAB. Genotype and phenotype analysis revealed insights into antibiotic resistance and carbohydrate metabolism in Lactobacillus strains.
Article
Food Science & Technology
Jose Maria Landete, Laura Plaza-Vinuesa, Cinthya Montenegro, Laura Santamaria, Ines Reveron, Blanca de las Rivas, Rosario Munoz
Summary: The metabolism of hydroxybenzoic and hydroxycinnamic acid derivatives in Lactobacillus plantarum involves a two-step pathway including esterase action and decarboxylation. Cloned esterase genes from L. plantarum were introduced into lactic acid bacteria to increase the bioavailability of healthy compounds in food. This study demonstrated the potential for utilizing these genes to enhance the metabolic pathways of phenolic acids in relevant lactic acid bacteria.
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
(2021)
Article
Veterinary Sciences
Peng Li, Liyun Zheng, Ya Qi, Zhipeng Liu, Encun Du, Jintao Wei, Zhengfan Zhang, Shuangshuang Guo, Binying Ding
Summary: Dietary lactobacillus fermentum (LF) and lactobacillus paracasei (LP) have beneficial effects on the intestinal health of broilers challenged with coccidia and Clostridium perfringens (CCP), by strengthening the intestinal barrier and alleviating inflammation.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ann Catherine Archer, Serva Peddha Muthukumar, Prakash Motiram Halami
Summary: The study investigated the effects of potential probiotic Lactobacillus fermentum on intestinal markers of inflammation in diabetes and obesity models. Results showed improved oral glucose tolerance, plasma insulin levels, and reduced inflammatory tone, suggesting Lact. fermentum as a potential probiotic for lifestyle-related disorders.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Nutrition & Dietetics
Erik M. Anderson, Jared M. Rozowsky, Brian J. Fazzone, Emilie A. Schmidt, Bruce R. Stevens, Kerri A. O'Malley, Salvatore T. Scali, Scott A. Berceli
Summary: Short-term dietary restriction can modulate the gut microbiome, causing relative dysbiosis, which is quickly restored after resuming a normal diet. This study highlights the importance of understanding the impact of nutritional interventions on the microbiome in order to improve surgical outcomes.
Article
Biotechnology & Applied Microbiology
Jian Yao, Lun Gui, Shaocheng Yin
Summary: A novel esterase gene was isolated from a soil metagenomic library, which exhibited high activity towards p-nitrophenyl esters with short acyl chains and p-nitrophenyl acetate. The enzyme showed optimal activity at pH 7.0 and 30 degrees C, and was stable in a broad pH range from 4.5 to 10.0 and temperature not exceeding 50 degrees C. The enzyme also demonstrated different substrate specificity from known esterases, showing activities towards a broad range of phenolic esters.
Article
Gastroenterology & Hepatology
Inmaculada Lopez-Almela, Marina Romani-Perez, Clara Bullich-Vilarrubias, Alfonso Benitez-Paez, Eva M. Gomez Del Pulgar, Ruben Frances, Gerhard Liebisch, Yolanda Sanz
Summary: The study suggests that combining B. uniformis with wheat bran extract can effectively suppress obesity, restore metabolic pathways and immune responses, reduce fat accumulation and liver inflammation.
Article
Food Science & Technology
Matias Irineo Russo, Maria Claudia Abeijon-Mukdsi, Arlette Santacruz, Romina Ross, Aurelio Lopez Malo, Paola Gauffin-Cano, Roxana Beatriz Medina
Summary: This study aimed to evaluate the survival and maintenance of FE activity in microencapsulated Lactobacillus strains obtained by spray drying during storage at 4 degrees C and under simulated gastrointestinal tract conditions. The results showed that CRL1446 and CRL1231 powders were able to resist prolonged storage and GIT conditions, retaining FE activity and preserving their probiotic potential.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Food Science & Technology
Barbara Luciana Nacchio, Natividad Avila Hael, Roxana Beatriz Medina, Marisa Selva Garro
Summary: Fermentation by lactobacilli can enhance the production of desirable volatile compounds and reduce unpleasant compounds in soybean paste. Consumers tend to prefer fermented samples with sweet and acid aromas similar to yogurt.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Nutrition & Dietetics
Emanuel Fabersani, Antonela Marquez, Matias Russo, Romina Ross, Sebastian Torres, Cecilia Fontana, Edoardo Puglisi, Roxana Medina, Paola Gauffin-Cano
Summary: This study found that lactic acid bacteria with different adipo- and immuno-modulatory capacities had different effects on metabolic and immunological parameters in high-fat-diet-induced obese mice. CRL1446 and CRL1434 strains could be used as adjuvant probiotics for nutritional treatment to obesity and overweight, while CRL431 strain may be more beneficial in pathologies that require immune system regulation.
FRONTIERS IN NUTRITION
(2021)
Review
Gastroenterology & Hepatology
Eline Margrete Randulff Hillestad, Aina van der Meeren, Bharat Halandur Nagaraja, Ben Rene Bjorsvik, Noman Haleem, Alfonso Benitez-Paez, Yolanda Sanz, Trygve Hausken, Guelen Arslan Lied, Arvid Lundervold, Birgitte Berentsen
Summary: This comprehensive review discusses the major features of irritable bowel syndrome and its interaction with gut microbiota, clinical microbiota-altering treatments such as low FODMAP diet and fecal microbiota transplantation, neuroimaging and microbiota analysis methods, and current and future challenges in big data analysis in IBS.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Editorial Material
Nutrition & Dietetics
Sebastian Torres, Roxana Beatriz Medina, Maria Isabel Vasallo Morillas, Maria Ines Isla, Paola Gauffin-Cano
FRONTIERS IN NUTRITION
(2021)
Article
Biochemical Research Methods
Pedro Sanchez-Sanchez, Francisco J. Santonja, Alfonso Benitez-Paez
Summary: Microbiome research is rapidly advancing, and there is a need for novel methods to assess the stability of microbiota data. The mu STASIS package provides a multifunctional framework to evaluate individual-centered microbiota stability, dealing with the sparse and compositional nature of microbiota data. It is a useful tool for assessing the stability of gut microbiota.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Gastroenterology & Hepatology
Alfonso Benitez-Paez, Annick Hartstra, Max Nieuwdorp, Yolanda Sanz
Summary: This study analyzed the changes in the human gut microbiota during a fecal microbiota transplantation (FMT) trial using nanopore technology. The results show that the engraftment of donors' microbiota is correlated with the improvement of metabolic health in recipients. Furthermore, the study revealed that assessing nucleotide frequencies can successfully explore the engraftment of donor bacterial strains. The findings indicate that factors such as baseline gut microbiota configuration, sex, and age of donors should be considered for successful FMT in humans.
Article
Microbiology
Estefania Andrada, Monica Adriana Mechoud, Maria Claudia Abeijon-Mukdsi, Elsa Patricia Chagra Dib, Santiago Cervino, Adriana Perez Chaia, Roxana Beatriz Medina
Summary: In this study, three FAE+ L. johnsonii strains were isolated and compared as silage inoculants. The results showed that L. johnsonii ETC187 had better inoculant and probiotic abilities. Although L. johnsonii ETC187 significantly reduced ADF, L. johnsonii ETC150 and CRL1446 not only induced similar ADF reduction, but also reduced dry matter loss.
Review
Nutrition & Dietetics
Paula Hernandez-Calderon, Lara Wiedemann, Alfonso Benitez-Paez
Summary: The investigation of the human gut microbiome has helped us understand its relevance for human health and the potential for targeted dietary strategies to combat non-communicable diseases. However, the success of dietary interventions for weight control is limited, highlighting the importance of considering an individual's gut microbiota composition to achieve significant adiposity reduction and improve metabolic status through personalized nutrition.
FRONTIERS IN NUTRITION
(2022)
Article
Multidisciplinary Sciences
Antonela Marquez, Estefania Andrada, Matias Russo, Maria Lujan Bolondi, Emanuel Fabersani, Roxana Medina, Paola Gauffin-Cano
Summary: This study aimed to design functional fermented goat milk with probiotic potential for metabolic diseases. The researchers characterized indigenous lactobacilli from goat dairy products that target improving the inflammatory, lipid, and glycemic profile. Fermented goat milk incorporating these lactobacilli showed positive effects on inhibiting alpha-glucosidase, bile salts hydrolase activity, cholesterol assimilation, and reducing triglyceride percentage. The oral administration of these lactobacilli to obese mice resulted in significant weight loss and improved hyperglycemia and hyperlipemia.
