期刊
NPG ASIA MATERIALS
卷 8, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/am.2016.130
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资金
- INNOPOLIS Foundation grant - Korean government (Ministry of Science, ICT & Future Planning) through WINOVA [2014DD023]
- Development of High Medical Technology Project of KHIDI, Korea [HI14C2755]
- Cooperative Research Program for Agriculture Science and Technology Development [PJ009956]
- Rural Development Administration, Republic of Korea
- National Research Foundation of korea grant - Korean Government [NRF-2016M3A9B4919616]
- Chungnam National University
- Ministry of Science, ICT & Future Planning, Republic of Korea [2014DD023] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Three-dimensional (3D) cell culture systems have promising applications compared with conventional two-dimensional cell culture systems. Herein, we report a facile method for the formation of 3D spheroids using novel thermo-reversible polysaccharide-based hydrogels. A series of thermo-reversible hydrogels consisting of N-acyl glycol chitosans (NAGCs) are synthesized through a simple N-acylation reaction, and the degree of acylation is finely tuned to obtain adequate thermo-reversible properties and gel stability. Among the NAGCs, N-hexanoyl glycol chitosan is the most thermo-sensitive and is highly effective for forming multi-cellular spheroids when used to coat the surfaces of cell culture dishes. Cell spheroids are effectively formed at various cell concentrations, and their spheroid shape and cellular functions are well maintained for longer times. The hydrogel culture system is also useful for co-cultures that mimic a biological microenvironment. Our thermo-reversible hydrogels may offer a convenient method for the development of in vitro 3D cell culture systems to provide enhanced performance in tissue regeneration, organ-on-chips, drug screening research and other biomedical applications.
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