Article
Engineering, Environmental
Hao Ding, Jie Ding, Qingnian Liu, Junxin Lin, Mengying He, Xinyu Wu, Xiaoying Chen, Changchen Xiao, Tanchen Ren, Yang Zhu, Changyou Gao, Xinyang Hu, Jian'an Wang
Summary: The use of a novel injectable hydrogel encapsulating mesenchymal stem cells (MSCs) provides a promising strategy for improving their survival and enhancing cardiac function in myocardial infarction (MI) repair. The hydrogel not only scavenges reactive oxygen species (ROS) but also generates oxygen, protecting MSCs from oxidative stress and improving their viability. The encapsulated MSCs effectively engraft in the infarcted heart, reducing apoptosis, promoting angiogenesis, and reducing fibrosis, ultimately improving cardiac functions.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Biomedical
Shuoji Zhu, Changjiang Yu, Nanbo Liu, Mingyi Zhao, Zerui Chen, Jian Liu, Ge Li, Huanlei Huang, Huiming Guo, Tucheng Sun, Jimei Chen, Jian Zhuang, Ping Zhu
Summary: The study found that using GelMA-O5/rGO hydrogel encapsulating UCMSCs could significantly improve damaged myocardial tissue and reconstruct myocardial function, which may be a promising therapeutic strategy for cardiac repair.
BIOACTIVE MATERIALS
(2022)
Article
Medicine, Research & Experimental
Shi Hua Tan, Sze Jie Loo, Yu Gao, Zhong Hao Tao, Li Ping Su, Chen Xu Wang, Sophia L. Zhang, Yong Hui Mu, Ying Hua Cui, Desiree Abdurrachim, Wei Hsin Wang, Janise Lalic, Kheng Choon Lim, Jun Bu, Ru San Tan, Teck Hock Lee, Jianyi Zhang, Lei Ye
Summary: In a porcine model of myocardial infarction, co-treatment with Tb4-microspheres and hiPSC-CMs improved the engraftment and reparative potency of the transplanted hiPSC-CMs, induced vasculogenesis and proliferation of cardiomyocytes and endothelial cells, improved left ventricular systolic function, and reduced infarct size. HiPSC-CM implantation did not increase the incidence of ventricular arrhythmias and did not induce tumorigenesis in the immunosuppressed pigs.
Review
Biotechnology & Applied Microbiology
Yang Xiao, Yihuan Chen, Chunlai Shao, Yaning Wang, Shijun Hu, Wei Lei
Summary: Myocardial infarction is a common cardiovascular disease caused by ischemia leading to cardiomyocyte damage and scar tissue formation. Current research focuses on improving the transplantation efficacy of PSC-CMs to enhance the treatment of myocardial infarction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Emma Claire James, Eva Tomaskovic-Crook, Jeremy Micah Crook
Summary: The regenerative capacity of cardiomyocytes is insufficient for functional recovery, leading to ischaemic heart disease as a major cause of cardiovascular deaths. While human-induced pluripotent stem cells (hiPSCs) show potential for modeling heart disease and replacement therapy, challenges remain with the mixed populations and immature properties of hiPSC-derived cardiomyocytes, which are being addressed by innovative bioengineering technologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Chunxia Liu, Yong Wu, Hong Yang, Kunyan Lu, Haixin Zhang, Yuanyuan Wang, Jingjing Wang, Linan Ruan, Zhenya Shen, Qian Yu, Yanxia Zhang
Summary: Myocardial infarction (MI) is a common cardiovascular disease and a major cause of global mortality. Transplantation of exogenous cardiomyocytes (CMs) has emerged as a promising treatment option due to the limited regenerative capacity of endogenous CMs. In this study, an injectable hydrogel composed of alginate and fibrin was developed for localized delivery of neonatal CMs and the angiogenesis agent vascular endothelial growth factor (VEGF) to the infarcted area of the heart. The hydrogel exhibited good biocompatibility, enabling the retention and integration of transplanted CMs in the host myocardium. Additionally, the delivered VEGF promoted blood recovery, mitigating the ischemic microenvironment and improving the survival of transplanted CMs. Intramyocardial injection of this hydrogel resulted in angiogenesis, inhibition of fibrosis, and improved cardiac function, highlighting its potential for MI treatment.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Yu Jiang, Si-Jia Sun, Zhe Zhen, Rui Wei, Nannan Zhang, Song-Yan Liao, Hung-Fat Tse
Summary: The study found that a decellularized placenta scaffold contains multiple growth and angiogenic factors that enhance the maturation and survival of seeded hiPSC-CMs. Transplantation of a bioengineered cardiac patch (BCP) is superior to using placenta or hiPSC-CMs alone in reducing infarct size and improving cell retention and neovascularization.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Faizus Sazzad, Michal Kuzemczak, Engracia Loh, Wellington Wu, Theo Kofidis
Summary: Current techniques for myocardial restoration remain experimental and challenging, with limited success. The unique anatomy and function of the heart make it difficult to replicate or support using existing methods. Researchers are exploring new strategies to overcome the limitations of current treatment options, particularly in light of the equivocal results of cell-based therapies.
Article
Cardiac & Cardiovascular Systems
Xi Lou, Yawen Tang, Lei Ye, Danielle Pretorius, Vladimir G. Fast, Asher M. Kahn-Krell, Jue Zhang, Jianhua Zhang, Aijun Qiao, Gangjian Qin, Timothy Kamp, James A. Thomson, Jianyi Zhang
Summary: The study demonstrates that human cardiac muscle patches containing three types of cardiac cells derived from human pluripotent stem cells significantly improved cardiac function, reduced infarct size, and promoted cardiac maturation in a pig model of myocardial infarction. The inclusion of cardiac fibroblasts in the cardiac patches enhanced the maturity and function of cardiac muscle cells. Transplantation of cardiac patches with cardiac fibroblasts resulted in better cardiac recovery and engraftment compared to patches without fibroblasts.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Klaus Neef, Florian Drey, Vera Lepperhof, Thorsten Wahlers, Jurgen Hescheler, Yeong-Hoon Choi, Tomo Saric
Summary: Co-transplantation of iPS-CM and MSC improves cardiac regeneration after myocardial damage, demonstrating the potential of combining multiple cell types for increasing the efficacy of future cardiac cell therapies. MSCs enhance the retention of iPS-CM and result in a significantly higher left ventricular ejection fraction (LVEF) when transplanted together.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Polymer Science
Maryam Torabi, Mohammad Foad Abazari, Shohreh Zare Karizi, Mina Kohandani, Nazanin Hajati-Birgani, Sara Norouzi, Fatemeh Nejati, Alireza Mohajerani, Tahereh Rahmati, Zakiye Mokhames
Summary: In this study, PLGA and PRP were used to fabricate nanofibrous scaffolds, and it was found that iPSCs cultured on PLGA-PRP nanofibers were more likely to differentiate into cardiomyocytes compared to PLGA. Multidimensional evaluations at the morphological, molecular gene, and protein expression levels indicated the potential of PLGA-PRP in cardiac tissue engineering.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2021)
Article
Chemistry, Multidisciplinary
Wei Liu, Nana Zhao, Qi Yin, Xiaoyi Zhao, Kangli Guo, Yifan Xian, Siwei Li, Chunlan Wang, Miaomiao Zhu, Yurong Du, Fu-Jian Xu, Changyong Wang, Jin Zhou
Summary: Significant progress has been made in the development of injectable functional biomaterials for cardiac regenerative therapy. The combination of natural alginate hydrogel and Au@Pt nanoparticles (Au@Pt/Alg hydrogel) as an injectable stem cell vector shows great potential. The Au@Pt/Alg hydrogel enhances the antioxidant and paracrine capabilities of stem cells and exhibits antioxidant, anti-inflammatory, and heart electrical integration effects in a rat myocardial infarction (MI) model. Moreover, it can effectively maintain the paracrine efficiency and pro-angiogenesis effects of stem cells in the infarcted area.
Review
Polymer Science
Wei Hu, Cui Yang, Xiaodan Guo, Yihong Wu, Xian Jun Loh, Zibiao Li, Yun-Long Wu, Caisheng Wu
Summary: Myocardial infarction is a serious disease and traditional treatment methods have limitations. Recent research has shown that injectable hydrogels can improve heart function and prognosis, providing new treatment strategies for MI.
Article
Engineering, Biomedical
Peng Wu, Xiyalatu Sai, Zhetao Li, Xing Ye, Li Jin, Guihuan Liu, Ge Li, Pingzhen Yang, Mingyi Zhao, Shuoji Zhu, Nanbo Liu, Ping Zhu
Summary: Induced pluripotent stem cell-derived cardiomyocytes have significant potential in the treatment of myocardial infarction, but their immaturity hinders their therapeutic efficacy. By synthesizing nano colloidal gelatin with collagen and combining it with a PI3K/AKT/mTOR pathway inhibitor, the maturity, function, and biocompatibility of the cardiomyocytes are improved.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Liang Peng, Muwei Li, Kang Zhao, Cao Ma, Haiyu Tang, Yan Li
Summary: Injectable HA-CS/beta-GP hydrogel-loaded MSCs show improved therapeutic effects on MI. The hydrogels exhibit good swelling and degradation properties, as well as biocompatibility. Implantation of the hydrogels significantly enhances the therapeutic effect of MSCs.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Anatomy & Morphology
Jenna Dumbleton, James G. Shamul, Bin Jiang, Pranay Agarwal, Haishui Huang, Xiaofeng Jia, Xiaoming He
Summary: This study investigates directed neural differentiation of murine ESCs using a non-planar microfluidic device to encapsulate cells in 3D microcapsules with alginate hydrogel shells. Modifications of the hydrogel shell facilitate early neurite extension and demonstrate potential for further neural differentiation into specialized neurons for therapeutic applications.
CELLS TISSUES ORGANS
(2022)
Article
Engineering, Biomedical
Bin Jiang, Wenquan Ou, James G. Shamul, Hao Chen, Sarah Van Belleghem, Samantha Stewart, Zhenguo Liu, John P. Fisher, Xiaoming He
Summary: The use of Rock inhibitor (RI) in 3D cardiac differentiation of human induced pluripotent stem cells (iPSCs) poses challenges, but reducing the RI concentration can improve efficiency and homogeneity, which is important for understanding and treating heart diseases.
BIOACTIVE MATERIALS
(2022)
Article
Oncology
Sanjay Mishra, Manish Charan, Rajni Kant Shukla, Pranay Agarwal, Swati Misri, Ajeet K. Verma, Dinesh K. Ahirwar, Jalal Siddiqui, Kirti Kaul, Neety Sahu, Kunj Vyas, Ayush Arpit Garg, Anum Khan, Wayne O. Miles, Jonathan W. Song, Nidhi Bhutani, Ramesh K. Ganju
Summary: S100A7 and cPLA2 are highly expressed in breast cancer patients and are associated with decreased overall survival. Further studies revealed that S100A7/RAGE signaling promotes the expression of cPLA2 and its oncogenic effects. Inhibiting cPLA2 can reduce S100A7-mediated tumor growth and metastasis, as well as increase immune cell infiltration.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Thermodynamics
Elyahb Allie Kwizera, Samantha Stewart, Md Musavvir Mahmud, Xiaoming He
Summary: Magnetic nanoparticles, especially superparamagnetic iron oxide nanoparticles, have garnered significant attention for their easy synthesis, functionalization, and control over size and shape. They have shown potential in various biomedical applications, including thermal treatments for cancer and enhancement of cryopreservation. Challenges in the use of magnetic nanoparticles in these biomedical applications are also discussed.
JOURNAL OF HEAT TRANSFER-TRANSACTIONS OF THE ASME
(2022)
Article
Neurosciences
Joaquin Muriel, Valeriy Lukyanenko, Tom Kwiatkowski, Sayak Bhattacharya, Daniel Garman, Noah Weisleder, Robert J. Bloch
Summary: Dysferlin, a transmembrane protein containing seven C2 domains, is crucial for membrane repair and Ca2+ release in skeletal muscle. Deletion of specific C2 domains results in impaired membrane repair and altered Ca2+ signaling, suggesting distinct functions for each domain. Localization to transverse tubules is not enough for normal Ca2+ signaling, with Ca2+ dependence of repair mediated by specific C2 domains.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Cell & Tissue Engineering
Michela Bruschi, Neety Sahu, Mamta Singla, Fiorella Grandi, Pranay Agarwal, Constance Chu, Nidhi Bhutani
Summary: This study introduces a quick and efficient method for generating mesenchymal stromal cells from human induced pluripotent stem cells in just 4 days, demonstrating their potential in cartilage regeneration and repair. The study highlights the stable differentiation and homogeneity of these cells, showcasing their enhanced gene expression, differentiation abilities, secretome, and immunomodulatory properties. The findings suggest that these hiMSCs derived from hiPSCs could be a promising source for clinical applications, providing a reliable and reproducible method for boosting the utilization of MSCs.
TISSUE ENGINEERING PART A
(2022)
Article
Chemistry, Multidisciplinary
Jiangsheng Xu, Yunhua Liu, Sheng Liu, Wenquan Ou, Alisa White, Samantha Stewart, Katherine H. R. Tkaczuk, Lee M. Ellis, Jun Wan, Xiongbin Lu, Xiaoming He
Summary: Colon and rectal cancers are leading causes of cancer-related deaths in the United States. Genomic analyses reveal the frequent hemizygous deletion of TP53 gene, an important tumor suppressor, in both cancers. Targeting TP53 directly has been unsuccessful, but the co-deletion of POLR2A gene with TP53 in these cancers suggests RNA interference with POLR2A siRNA as a potential strategy. However, efficient delivery of siRNA into the cytosol is a major challenge. To overcome this barrier, pH-responsive MetC-nanoparticles with a bomb effect are developed for effective cytosolic delivery of POLR2A siRNA, enhancing therapeutic efficacy for TP53-deficient cancer.
Article
Multidisciplinary Sciences
Rowan H. McLachlan, James T. Price, Agusti Munoz-Garcia, Noah L. Weisleder, Stephen J. Levas, Christopher P. Jury, Robert J. Toonen, Andrea G. Grottoli
Summary: Climate change poses a major threat to coral reefs, but certain resilient corals and genotypes of sensitive species can persist and cope well under future ocean conditions, provided atmospheric carbon dioxide levels are controlled.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Jiangsheng Xu, James G. Shamul, Elyahb Allie Kwizera, Xiaoming He
Summary: Mitochondria are critical organelles for producing energy in cells and can be targeted for cancer therapy. Mitochondria-targeting nanotechnologies have shown high efficacy for cancer treatment both in vitro and in vivo, and can be intelligently designed based on the characteristics of the tumor microenvironment.
Article
Cell Biology
Brian J. Paleo, Kevin E. McElhanon, Hannah R. Bulgart, Kassidy K. Banford, Eric X. Beck, Kristina M. Sattler, Briana N. Goines, Shelby L. Ratcliff, Kelly E. Crowe, Noah Weisleder
Summary: TRIM72/MG53-mediated membrane repair can partially compensate for sarcolemmal fragility in DMD and the loss of membrane repair leads to increased pathology.
Article
Genetics & Heredity
Pei-Chih Lee, Samantha Stewart, Olga Amelkina, Hannah Sylvester, Xiaoming He, Pierre Comizzoli
Summary: Intracellular trehalose delivery through CRNPs improves the dehydration tolerance of COCs, opening up new possibilities for oocyte storage and preservation of fertility at ambient temperatures.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Retraction
Chemistry, Multidisciplinary
Hai Wang, Pranay Agarwal, Gang Zhao, Guang Ji, Christopher M. Jewell, John P. Fisher, Xiongbin Lu, Xiaoming He
ACS CENTRAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Oceane Ballouhey, Marie Chapoton, Benedicte Alary, Sebastien Courrier, Nathalie Da Silva, Martin Krahn, Nicolas Levy, Noah Weisleder, Marc Bartoli
Summary: This study describes a new mouse model, Dysf (p.Y1159X/p.Y1159X), which carries several defects observed in dysferlinopathy patients and other published mouse models. This mutant mouse model is expected to be useful for testing various therapeutic approaches such as termination codon readthrough, pharmacological approaches, and exon skipping. Therefore, the data presented in this study strongly support the use of this animal model for the development of preclinical strategies for the treatment of dysferlinopathies.
Article
Engineering, Biomedical
Samantha Stewart, Wenquan Ou, Helim Aranda-Espinoza, Shaik O. Rahaman, Xiaoming He
Summary: Ovarian follicles develop in a regulated mechanical microenvironment, and disruptions to this microenvironment can cause infertility. The viscoelastic properties of ovarian tissue have not been extensively studied. This study characterizes the elastic and viscoelastic properties of ovarian tissue from both reproductive age groups of domestic cats and identifies possible connections between mechanical and compositional heterogeneities. These findings may have important implications for biomimetic follicle culture.
ACTA BIOMATERIALIA
(2023)
Review
Reproductive Biology
Pierre Comizzoli, Xiaoming He, Pei-Chih Lee
Summary: Preserving germ cells and gonadal tissues for the long term is critical in fertility treatment and animal population management. Current options at freezing temperatures are often constraining, but recent research suggests alternative strategies based on dehydration and storage at supra-zero temperatures. Further studies are needed for rehydration and reanimation of germ cells.
REPRODUCTION AND FERTILITY
(2022)
