Review
Genetics & Heredity
Christine Greil, Monika Engelhardt, Ralph Waesch
Summary: Cell cycle progression is tightly regulated by various protein kinases and proteolysis to maintain genomic stability. The spindle assembly checkpoint plays a crucial role in ensuring correct chromosome separation during mitosis. The ubiquitin ligase proteolysis is essential for these processes, with Cdh1 and Cdc20 being important regulators associated with tumorigenesis.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Mariam Huda, Seyma Nur Bektas, Baris Bekdas, Ayse Koca Caydasi
Summary: This study reveals that lysosomal signalling lipid PI3,5P(2) plays a role in the timely execution of mitotic exit and promotes it by impairing Kin4.
Article
Oncology
Christine Greil, Julia Felthaus, Marie Follo, Gabriele Ihorst, Daniel Ewerth, Julia Schueler, Dominik Schnerch, Justus Duyster, Monika Engelhardt, Ralph Waesch
Summary: Targeting mitosis by taxanes is a common chemotherapeutic approach in various solid tumors, but the combination of anti mitotic agents with a clinically approved PI or inhibitors of the APC/C and Mcl-1 shows promise in improving treatment response. The study revealed that the sequential combination of paclitaxel with the PI bortezomib enhanced cell death, with different effects observed in lung cancer and breast cancer due to entity-dependent factors such as the expression of antiapoptotic regulator Mcl-1.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Peter Tatrai, Fanni Gergely
Summary: This study shows the critical role of CDK5RAP2 and centrosomes in spindle formation during blood production. Lack of CDK5RAP2 leads to defective erythroid differentiation and the emergence of macrocytic anemia. The study suggests that disruption of centrosome and spindle function could contribute to the development of macrocytic anemias.
Article
Biology
Richard Wang, Camilla Ascanelli, Ahmed Abdelbaki, Alex Fung, Tim Rasmusson, Iacovos Michaelides, Karen Roberts, Catherine Lindon
Summary: In this study, a PROTAC derivative of AURKA inhibitor MLN8237 was characterized for its efficient and specific destruction of AURKA. The subcellular localization and differential sensitivity of AURKA pools to PROTAC action were demonstrated, highlighting the potential of this new class of degrader compounds for targeting specific subpopulations within cells. The findings suggest that PROTACs can modulate AURKA functions in distinct cellular compartments, providing a novel approach for therapeutic intervention.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Surbhi Lambhate, Debanjan Bhattacharjee, Nishant Jain
Summary: Research suggests that ROS progressively increases during mitosis, and IDH2 ubiquitination is linked to ROS production during mitosis, with APC/C CDH1 playing a regulatory role.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Thao P. Phan, Aubrey L. Maryniak, Christina A. Boatwright, Junsu Lee, Alisa Atkins, Andrea Tijhuis, Diana C. J. Spierings, Hisham Bazzi, Floris Foijer, Philip W. Jordan, Travis H. Stracker, Andrew J. Holland
Summary: Depletion of centrosome proteins in neural progenitor cells (NPCs) prolongs mitosis and increases TP53-mediated apoptosis in the developing brain. Activation of the mitotic surveillance pathway by mitotic delays is proposed to be the mechanism behind microcephaly caused by mutations in centrosome genes. Loss of 53BP1 or USP28 can rescue cell death after delayed mitosis in NPCs, restoring NPC proliferation and brain size.
Article
Plant Sciences
Ting Zhang, Shuang-Hui Zhao, Yan He
Summary: In this study, a meiotic cell-cycle controller ZmTDM1 was identified in maize, which plays a crucial role in the termination of the meiotic cell cycle. ZmTDM1 interacts with RING-type E3 ubiquitin ligase and acts as a subunit of the APC/C E3 ubiquitin ligase complex. This study provides insights into the genetic controls underlying the meiotic cell cycle in maize.
Article
Cell Biology
Thomas Tischer, Jing Yang, David Barford
Summary: The control of protein abundance is critical during mitosis, and this study reveals that the APC/C not only regulates mitotic progression, but also plays a role in spindle assembly through its interaction with Cep152.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mara Esposito, Gustavo J. Gutierrez
Summary: The study reveals the interplay between USP13 and CDH1, showing that USP13 can stabilize CDH1 levels. Furthermore, it establishes the USP13-CDH1-Aurora B axis as a new regulatory module essential for flawless cell cycle progression in mammalian cells.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Multidisciplinary Sciences
Su-Lin Zeng, Suraj S. Patel, Mo-Qi Lv, Daphne Zhu, Wen H. Shen, Li Liu
Summary: In this study, the novel oncogene STYK1/NOK's role in cell division was investigated. It was found that STYK1/NOK interacts directly with CDH1 and can affect cell division by targeting key components of the APC/C pathway. These findings provide mechanistic clues for the understanding of STYK1/NOK's roles in mitosis and cytokinesis.
Article
Cell Biology
Jeffrey C. Medley, Joseph R. DiPanni, Luke Schira, Blake M. Shaffou, Brandon M. Sebou, Mi Hye Song
Summary: Aberrant centrosome numbers in human cancers are closely associated with changes in levels of centrosome regulators. This study demonstrates that both APC/CFZR-1 and SCFSlimb/beta TrCP ubiquitin ligases cooperatively regulate the stability of ZYG-1/Plk4 protein in C. elegans through the D-box and SB motifs, ensuring proper centrosome formation and embryonic viability.
JOURNAL OF CELL SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Binshad Badarudeen, Ushma Anand, Swarnendu Mukhopadhyay, Tapas K. Manna
Summary: The centrosome is essential for maintaining genetic stability, ciliogenesis, and cell polarization, consisting of two centrioles that duplicate precisely once during each cell cycle. Recent advances have shown that dedicated ubiquitin ligase-dependent protein degradation machineries regulate centriole duplication by targeting different centriole proteins with intricate mechanisms. This review highlights the coordination of different ubiquitin ligase machineries in controlling centriole duplication.
Article
Cell Biology
Fabiola Mascanzoni, Roberta Iannitti, Antonino Colanzi
Summary: The dynamic association between the Golgi complex and the centrosome plays a crucial role in cellular processes such as cell polarization and division.
Article
Biochemistry & Molecular Biology
Xiaofu Cao, Adnan Shami Shah, Ethan J. Sanford, Marcus B. Smolka, Jeremy M. Baskin
Summary: Researchers have identified PLEKHA5 as a microtubule-resident protein that regulates the activity of the APC/C during mitosis. Depletion of PLEKHA5 disrupts the association between APC/C and the microtubule cytoskeleton, resulting in decreased activation of APC/C by its coactivator CDC20 and delayed mitotic progression.
ACS CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Richard J. Wall, David J. P. Ferguson, Aline Freville, Blandine Franke-Fayard, Declan Brady, Mohammad Zeeshan, Andrew R. Bottrill, Sally Wheatley, Andrew M. Fry, Chris J. Janse, Hiroyuki Yamano, Anthony A. Holder, David S. Guttery, Rita Tewari
SCIENTIFIC REPORTS
(2018)
Article
Cell Biology
Davide Gambarotto, Carole Pennetier, John M. Ryniawec, Daniel W. Buster, Delphine Gogendeau, Alix Goupil, Maddalena Nano, Anthony Simon, Damien Blanc, Victor Racine, Yuu Kimata, Gregory C. Rogers, Renata Basto
DEVELOPMENTAL CELL
(2019)
Review
Cell Biology
Yuu Kimata
TRENDS IN CELL BIOLOGY
(2019)
Review
Cell Biology
Meghna Kataria, Hiroyuki Yamano
Article
Biochemistry & Molecular Biology
Joao Barbosa, Torcato Martins, Tanja Bange, Li Tao, Carlos Conde, Claudio Sunkel
Article
Biochemistry & Molecular Biology
Kazuyuki Fujimitsu, Hiroyuki Yamano
Article
Biochemistry & Molecular Biology
Hadi Boukhatmi, Torcato Martins, Zoe Pillidge, Tsveta Kamenova, Sarah Bray
Review
Biochemistry & Molecular Biology
Yuu Kimata, Maite Leturcq, Rajaguru Aradhya
Article
Biochemistry & Molecular Biology
Takashi Ochi, Valentina Quarantotti, Huawen Lin, Jerome Jullien, Ivan Rosa e Silva, Francesco Boselli, Deepak D. Barnabas, Christopher M. Johnson, Stephen H. McLaughlin, Stefan M. Freund, Andrew N. Blackford, Yuu Kimata, Raymond E. Goldstein, Stephen P. Jackson, Tom L. Blundell, Susan K. Dutcher, Fanni Gergely, Mark van Breugel
Article
Biochemistry & Molecular Biology
Kazuyuki Fujimitsu, Hiroyuki Yamano
Summary: The study reveals an interplay between PLK1 enzyme and PP2A-B56 phosphatase on the Apc1 subunit of the APC/C, controlling its activity and mitotic progression through phosphorylation-dependent feedback regulation. PLK1 promotes the formation of APC/C-Cdc20 via phosphorylation, while PP2A-B56 promotes dissociation of PLX1 through dephosphorylation, ultimately regulating the activity and phosphorylation status of APC/C.
Article
Biochemistry & Molecular Biology
Torcato Martins, Yao Meng, Boguslawa Korona, Richard Suckling, Steven Johnson, Penny A. Handford, Susan M. Lea, Sarah J. Bray
Summary: Accurate Notch signalling is crucial for development and homeostasis, with recent studies showing the importance of the conserved N-terminal C2 domain in Notch ligands. Mutations in the beta 1-2 loop of the Delta C2 domain affect phospholipid binding and compromise ligand function in vivo. These findings highlight the essential role of C2 domain phospholipid binding in fine-tuning the balance of trans and cis ligand-receptor interactions for robust signalling.
Article
Biochemistry & Molecular Biology
Alexis Leah Braun, Francesco Meghini, Gema Villa-Fombuena, Morgane Guermont, Elisa Fernandez-Martinez, Zhang Qian, Maria Dolores Martin-Bermudo, Acaimo Gonzalez-Reyes, David Moore Glover, Yuu Kimata
Summary: This study reveals the importance of proper transport of centrosomes into the oocyte during early Drosophila oogenesis for maintaining oocyte fate. The control of the Anaphase Promoting Complex/Cyclosome (APC/C) and Polo/Plk1 kinase plays a critical role in this process, with mutations affecting centrosome transport and leading to loss of oocyte identity. These findings may have implications for understanding reproductive processes in other animals due to the conserved roles of APC/C and Polo.
Article
Biochemistry & Molecular Biology
Francesco Meghini, Torcato Martins, Qian Zhang, Nicolas Loyer, Michelle Trickey, Yusanjiang Abula, Hiroyuki Yamano, Jens Januschke, Yuu Kimata
Summary: A functional centrosome is crucial for animal development and physiology. In addition to regulating centriole duplication, ubiquitin-mediated proteolysis plays a vital role in controlling the precise regulation of centrosome. The research conducted on Drosophila demonstrates that APC/C-Fzr controls the levels of Spd2 to affect neural stem cell-specific asymmetric PCM recruitment and microtubule nucleation at interphase centrosomes. Furthermore, APC/C-Fzr-dependent Spd2 degradation facilitates the accumulation of Polo-dependent Spd2 phosphorylation for PCM recruitment.