Article
Oncology
Yao Kong, Yuming Luo, Shangyou Zheng, Jiabin Yang, Dingwen Zhang, Yue Zhao, Hanhao Zheng, Mingjie An, Yan Lin, Le Ai, Xiayao Diao, Qing Lin, Changhao Chen, Rufu Chen
Summary: This study identified a novel circRNA, circARFGEF2, which is upregulated in KRASG12D pancreatic ductal adenocarcinoma (PDAC) and positively associated with lymph node metastasis. The biogenesis of circARFGEF2 is activated by the alternative splicing factor QKI-5 and it promotes metastasis by sponging miR-1205 and activating JAK2.
Article
Biochemistry & Molecular Biology
Huan Zhang, Haixin Yu, Dianyuan Ren, Yan Sun, Feng Guo, Hongkun Cai, Chen Zhou, Yingke Zhou, Xin Jin, Heshui Wu
Summary: Tobacco smoking induces epigenetic modifications to promote pancreatic cancer development, with CBX3 acting as a key regulator in this process.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yuchen Bai, Carolin Gotz, Ginevra Chincarini, Zixuan Zhao, Clare Slaney, Jarryd Boath, Luc Furic, Christopher Angel, Stephen M. Jane, Wayne A. Phillips, Steven A. Stacker, Camile S. Farah, Charbel Darido
Summary: Subtype-specific treatment regimens are currently lacking for heterogeneous head and neck cancer (HNC). An integrated analysis of HNC subtypes using single-cell sequencing and proteome profiles reveals an epithelial-mesenchymal transition (EMT) signature and its correlation with PI3K/mTOR signaling pathway. YBX1 phosphorylation downstream of PI3K/mTOR pathway inhibits basal-like cancer cell proliferation, while acting as a safeguard against the proliferation-to-invasion switch in mesenchymal-like epithelial cancer cells. Phospho-YBX1 that is mutually exclusive to partial-EMT emerges as a prognostic marker for overall patient outcomes. These findings provide a promising therapeutic approach against HNC by sensitizing mesenchymal cancer cells to PI3K/mTOR inhibitors through shifting them towards a basal-like subtype.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Chemical
Erika Nallely Orendain-Jaime, Nicolas Serafin-Higuera, Ana Gabriela Leija-Montoya, Gustavo Martinez-Coronilla, Misael Moreno-Trujillo, Fausto Sanchez-Munoz, Armando Ruiz-Hernandez, Javier Gonzalez-Ramirez
Summary: Cancer is a deadly disease associated with virus-induced infections, where miRNAs play a significant role and could serve as biomarkers. sRNAs are also important in gene expression and bacterial infections, potentially related to cancer.
Article
Cell Biology
Weichen Wang, Longlong Wang, Mengyue Yang, Chunwei Wu, Rui Lan, Weiwei Wang, Yuze Li
Summary: Studies have shown that circ-SIRT1 restrains cardiac hypertrophy by activating SIRT1 to promote autophagy, suggesting circ-SIRT1 as a promising target for alleviating CH.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Afeez Adekunle Ishola, Chian-Shiu Chien, Yi-Ping Yang, Yueh Chien, Aliaksandr A. Yarmishyn, Ping-Hsing Tsai, Jerry Chieh-Yu Chen, Po-Kuei Hsu, Yung-Hung Luo, Yuh-Min Chen, Kung-Hou Liang, Yuan-Tzu Lan, Teh-Ia Huo, Hsin- Ma, Ming-Teh Chen, Mong-Lien Wang, Shih-Hwa Chiou
Summary: Lung cancers, particularly non-small cell lung cancers, have a poor prognosis. A circular noncoding RNA called C190 has been identified as a negative prognostic biomarker for lung cancer, and this study explores its mechanistic function and potential as a therapeutic target for NSCLC.
Article
Biochemistry & Molecular Biology
Baoqing Tian, Jiandong Liu, Nasha Zhang, Yemei Song, Yeyang Xu, Mengyu Xie, Bowen Wang, Hui Hua, Yue Shen, Yankang Li, Ming Yang
Summary: Research has shown that high expression of clinically relevant snoRNA SNORD12B in ESCC patients is associated with poor prognosis. SNORD12B interacts with PP-1α, disrupting the interaction between 14-3-3ζ and PP-1α, thus promoting the development of ESCC.
Article
Biochemistry & Molecular Biology
Carline Chaves-Almagro, Johanna Auriau, Alizee Dortignac, Pascal Clerc, Hubert Lulka, Simon Deleruyelle, Fabrice Projetti, Jessica Nakhle, Audrey Frances, Judit Berta, Veronique Gigoux, Daniel Fourmy, Marlene Dufresne, Anne Gomez-Brouchet, Julie Guillermet-Guibert, Pierre Cordelier, Bernard Knibiehler, Ralf Jockers, Philippe Valet, Yves Audigier, Bernard Masri
Summary: This study investigated the expression and protumoral function of apelin and its receptor APJ in human pancreatic adenocarcinoma. It was found that apelin and APJ were co-expressed by tumor cells in the invasive stage, and apelin promoted cancer cell proliferation, migration, and glucose uptake. Blocking the apelin receptor reduced cancer cell proliferation and pancreatic tumor burden.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Masatomo Chiba, Kenichi Miyata, Hikaru Okawa, Yoko Tanaka, Koji Ueda, Hiroyuki Seimiya, Akiko Takahashi
Summary: Senescent cells secrete inflammatory proteins and sEVs, which contribute to age-related diseases. This study reveals that YBX1 is involved in loading SATII RNA into sEVs, and the spread of SATII RNA through sEVs promotes the expression of inflammatory SASP genes. Additionally, high expression of YBX1 is associated with poor prognosis in breast and ovarian cancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Shu Xu, San-Lin Lei, Kui-Jie Liu, Shen-Gen Yi, Zhu-Lin Yang, Hong-Liang Yao
Summary: Pancreatic cancer (PC) is a common and lethal cancer with poor prognosis and limited treatment options. This study found that circSFMBT1 and PAK1 were up-regulated, while miR-330-5p was down-regulated in PC tissues and cells, promoting proliferation and metastasis of PC cells through the regulation of the miR-330-5p/PAK1 pathway.
CANCER GENE THERAPY
(2021)
Article
Cell Biology
Yuan He, HongQin Yue, Ying Cheng, Zhilong Ding, Zhen Xu, Chunyang Lv, Zheng Wang, Jing Wang, Chenglong Yin, Huihui Hao, Chuang Chen
Summary: Long noncoding RNA KCNK15-AS1 is down-regulated in pancreatic cancer cells, and its overexpression inhibits cell proliferation, migration, and induces apoptosis. Mechanistically, ALKBH5 induces m(6)A demethylation of KCNK15-AS1, leading to up-regulation and subsequent inhibition of AKT pathway through regulating KCNK15 and PTEN.
CELL DEATH & DISEASE
(2021)
Article
Biotechnology & Applied Microbiology
Yunjin Zhang, Qingrui Zhao, Shenghan Su, Lingfeng Dan, Xuebei Li, Yu Wang, Yuqing Lin, Zhen Tian, Changhao Sun, Huimin Lu
Summary: Chronic exposure to high concentrations of circulating palmitic acid and stearic acid can impair beta cell function, leading to the development of type 2 diabetes. This study revealed differential expression of circRNAs involved in beta cell dysfunction induced by palmitic acid and stearic acid, as well as identified potential therapeutic targets for treating high fat diet-induced beta cell injury.
Article
Multidisciplinary Sciences
Xudong Huang, Ling Pan, Zhixiang Zuo, Mei Li, Lingxing Zeng, Rui Li, Ying Ye, Jialiang Zhang, Guandi Wu, Ruihong Bai, Lisha Zhuang, Lusheng Wei, Yanfen Zheng, Jiachun Su, Junge Deng, Shuang Deng, Shaoping Zhang, Shihao Zhu, Xu Che, Chengfeng Wang, Chen Wu, Rufu Chen, Dongxin Lin, Jian Zheng
Summary: LINC00842 plays a role in promoting the malignancy of pancreatic ductal adenocarcinoma by regulating the acetylation status of PGC-1 alpha, leading to metabolic reprogramming in cancer cells.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Zhen Zheng, Wenlong Zuo, Rongrong Ye, Jason William Grunberger, Nitish Khurana, Xianyu Xu, Hamidreza Ghandehari, Fenglei Chen
Summary: This study investigates the relationship between autophagy and apoptosis induced by silica nanoparticles (SNPs) in ovarian granulosa cells. The results show that intratracheal instillation of 25.0 mg/kg body weight (b.w.) of 110 nm spherical Stober SNPs causes ovarian granulosa cell apoptosis in follicles in vivo. In vitro, SNPs mainly internalize into the lumens of lysosomes in primary cultured ovarian granulosa cells. SNPs induce cytotoxicity by decreasing cell viability and increasing apoptosis in a dose-dependent manner, activating autophagy and blocking autophagic flux.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Yang Chen, Sujuan Yang, Jena Tavormina, Desiree Tampe, Michael Zeisberg, Huamin Wang, Krishnan K. Mahadevan, Chang-Jiun Wu, Hikaru Sugimoto, Chia-Chi Chang, Robert R. Jenq, Kathleen M. McAndrews, Raghu Kalluri
Summary: Pancreatic cancer cells produce a unique Col1 homotrimer which promotes tumor growth and affects tumor microbiome and immunity. Deletion of Col1 homotrimer improves survival in mice and enhances the efficacy of anti-PD-1 immunotherapy.
Article
Biotechnology & Applied Microbiology
Tatsunori Suzuki, Takahiro Kishikawa, Tatsuyuki Sato, Norihiko Takeda, Yuki Sugiura, Takahiro Seimiya, Kazuma Sekiba, Motoko Ohno, Takuma Iwata, Rei Ishibashi, Motoyuki Otsuka, Kazuhiko Koike
Summary: Mutational activation of the KRAS gene is an early event in the carcinogenesis of almost all PDAC. Research shows that KRAS mutation increases the dependency on glucose and glutamine and reduces intracellular levels of amino acids, necessitating higher autophagic flux for cell viability.
CANCER GENE THERAPY
(2022)
Article
Gastroenterology & Hepatology
Hiroyuki Kato, Keisuke Tateishi, Hiroaki Fujiwara, Takuma Nakatsuka, Keisuke Yamamoto, Yotaro Kudo, Yoku Hayakawa, Hayato Nakagawa, Yasuo Tanaka, Hideaki Ijichi, Motoyuki Otsuka, Dosuke Iwadate, Hiroki Oyama, Sachiko Kanai, Kensaku Noguchi, Tatsunori Suzuki, Tatsuya Sato, Ryunosuke Hakuta, Kazunaga Ishigaki, Kei Saito, Tomotaka Saito, Naminatsu Takahara, Takahiro Kishikawa, Tsuyoshi Hamada, Ryota Takahashi, Koji Miyabayashi, Suguru Mizuno, Hirofumi Kogure, Yousuke Nakai, Yoshihiro Hirata, Atsushi Toyoda, Kazuki Ichikawa, Wei Qu, Shinichi Morishita, Junichi Arita, Mariko Tanaka, Tetsuo Ushiku, Kiyoshi Hasegawa, Mitsuhiro Fujishiro, Kazuhiko Koike
Summary: Our study identified the MNX1-HNF1B axis to be biologically significant in IPMN lineages, and this axis is crucial for the regulation of genes such as MYC, SOX9, and OLFM4.
Article
Biochemistry & Molecular Biology
Kazuyoshi Funato, Motoyuki Otsuka, Kazuma Sekiba, Yu Miyakawa, Takahiro Seimiya, Chikako Shibata, Takahiro Kishikawa, Mitsuhiro Fujishiro
Summary: DNA repair processes are potential therapeutic targets for cancer treatment. This study reveals that HBV-associated HCC with a deficiency in the Smc5/6 complex is more susceptible to PARP inhibitors due to impaired homologous recombination. The findings suggest a potential treatment strategy for HBV-related HCC.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Oncology
Koji Miyabayashi, Hideaki Ijichi, Mitsuhiro Fujishiro
Summary: Pancreatic cancer is influenced by the microbiome, which affects the tumor microenvironment and immune system, ultimately impacting the efficacy of chemotherapy. Understanding the role and mechanisms of the microbiome can lead to new therapeutic strategies and biomarkers.
Article
Oncology
Chikako Shibata, Motoyuki Otsuka, Takahiro Seimiya, Takahiro Kishikawa, Kazunaga Ishigaki, Mitsuhiro Fujishiro
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Chikako Shibata, Motoyuki Otsuka, Takahiro Seimiya, Kazunaga Ishigaki, Yu Miyakawa, Takahiro Kishikawa, Mitsuhiro Fujishiro
Summary: The size of EVs is influenced by different types of cancers, with EVs derived from pancreatic cancer cells being smaller than those from non-cancer cells. Low glucose conditions reduce EVs' size distribution and increase levels of unsaturated fatty acids in EVs. Increased uptake of smaller EVs by recipient cells may lead to enhanced functional outcomes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Oncology
Hiroyuki Kato, Keisuke Tateishi, Dosuke Iwadate, Keisuke Yamamoto, Hiroaki Fujiwara, Takuma Nakatsuka, Yotaro Kudo, Yoku Hayakawa, Hideaki Ijichi, Motoyuki Otsuka, Takahiro Kishikawa, Ryota Takahashi, Koji Miyabayashi, Yousuke Nakai, Yoshihiro Hirata, Atsushi Toyoda, Shinichi Morishita, Mitsuhiro Fujishiro
Summary: This study discovered the correlation between 3D genome structures and molecular subtypes of pancreatic cancer (PC) through Hi-C analysis. They found that HNF1B regulates the expression of related genes by influencing the 3D structures. These findings are significant for a better understanding of the molecular features of PC.
Article
Multidisciplinary Sciences
Takahiro Seimiya, Tatsunori Suzuki, Takuma Iwata, Takahiro Kishikawa, Kazuma Sekiba, Chikako Shibata, Kazunaga Ishigaki, Hiroaki Fujiwara, Hiroki Oyama, Sachiko Kanai, Tatsuya Sato, Yousuke Nakai, Rei Ishibashi, Masaru Moriyama, Ryo Nakagawa, Hideaki Ijichi, Motoyuki Otsuka, Kazuhiko Koike
Summary: Pancreatic ductal adenocarcinoma (PDAC) is difficult to diagnose and has a poor prognosis. Human satellite II (HSATII) RNA, highly expressed in human PDAC, may serve as a biomarker for PDAC. We developed a convenient method, tandem repeat amplification by nuclease protection (TRAP) combined with droplet digital PCR (ddPCR), to measure serum HSATII RNA level with high sensitivity and reproducibility. By simultaneously measuring serum miR-21-5p level, we refined the method and constructed a PDAC-Index that accurately discriminates PDAC patients. The PDAC-Index was clinically validated as a supportive test in challenging diagnostic cases and showed satisfactory diagnostic performance for routine PDAC detection.
Editorial Material
Oncology
Hideaki Ijichi
Letter
Medicine, General & Internal
Elif Hindie
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Letter
Medicine, General & Internal
Motoyuki Otsuka
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Medicine, Research & Experimental
Satoshi Kawamura, Yuki Matsushita, Shigeyuki Kurosaki, Mizuki Tange, Naoto Fujiwara, Yuki Hayata, Yoku Hayakawa, Nobumi Suzuki, Masahiro Hata, Mayo Tsuboi, Takahiro Kishikawa, Hiroto Kinoshita, Takuma Nakatsuka, Masaya Sato, Yotaro Kudo, Yujin Hoshida, Atsushi Umemura, Akiko Eguchi, Tsuneo Ikenoue, Yoshihiro Hirata, Motonari Uesugi, Ryosuke Tateishi, Keisuke Tateishi, Mitsuhiro Fujishiro, Kazuhiko Koike, Hayato Nakagawa
Summary: Inhibition of sterol regulatory element-binding proteins (SREBPs) is not an effective approach for treating nonalcoholic steatohepatitis (NASH), as it exacerbates liver injury and liver cancer development despite reducing hepatic steatosis. Additionally, SREBP inhibition alters lipid composition, causes endoplasmic reticulum stress and hepatocyte injury, but these effects can be mitigated by supplementation with phosphatidylcholines.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Meeting Abstract
Oncology
Ryota Takahashi, Hideaki Ijichi, Kazuhiko Koike
Meeting Abstract
Oncology
Yukino Oshima, Makoto Sano, Yoshimi Ichimaru, Ichie Kajiwara, Tomoaki Itaya, Tomoya Kuramochi, Jinsuk Kim, Hideaki Ijichi, Yukimoto Ishii, Takahiro Suzuki