Article
Oncology
Rati Lama, Chao Xu, Samuel L. Galster, Javier Querol-Garcia, Scott Portwood, Cory K. Mavis, Federico M. Ruiz, Diana Martin, Jin Wu, Marianna C. Giorgi, Jill Bargonetti, Eunice S. Wang, Francisco J. Hernandez-Ilizaliturri, Gerald B. Koudelka, Sherry R. Chemler, Ines G. Munoz, Xinjiang Wang
Summary: This study identifies MMRi62 as a novel MDM2-MDM4-targeting agent that can induce apoptosis in leukemia cells, including those with non-functional p53.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tamotsu Nishida
Summary: PARIS, a substrate of the ubiquitin E3 ligase parkin, is involved in various pathological processes. SENP3 interacts with PARIS and enhances its ubiquitination independently of SUMOylation. MDM2 activation via the PI3K/AKT pathway is involved in PARIS ubiquitination in parkin-deficient cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Hongbiao Ran, Chunyan Li, Ming Zhang, Jincheng Zhong, Hui Wang
Summary: Ubiquitination, a post-transcriptional modification, plays a crucial role in protein degradation and is involved in various physiological and pathological processes, including animal adipogenesis. The E3 ligases have been found to regulate adipogenesis, but their role in the recognition and connection between substrate and ubiquitin during ubiquitination has been overlooked. This review summarizes the regulatory and modification targets of E3 ligases in animal adipogenesis, explains the regulatory mechanisms in lipogenic pathways, and analyzes existing positive results, providing valuable research directions for further understanding the regulatory mechanisms of E3 ligases in animal adipogenesis.
Review
Cell Biology
Albano Toska, Nikita Modi, Lin-Feng Chen
Summary: RUNX3 is a transcription factor involved in cell proliferation and development. It can act both as a tumor suppressor and an oncogene in different cancers. The tumor suppressor function of RUNX3 is mainly attributed to its ability to suppress cancer cell proliferation through ubiquitination and proteasomal degradation, while it can also be inactivated through the same mechanism.
Article
Biochemistry & Molecular Biology
Jiayi Yu, Lu Kang, Yuanyuan Li, Changai Wu, Chengchao Zheng, Pei Liu, Jinguang Huang
Summary: This study identified that RGLG1 and RGLG2 negatively regulate the drought stress tolerance mediated by MAPKKK18 in Arabidopsis by promoting the degradation of MAPKKK18 through ubiquitination. Deletion of RGLG1 and RGLG2 stabilizes MAPKKK18 and enhances drought stress tolerance in MAPKKK18-overexpression plants.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shidong Zhao, Wenwen Ru, Xinyan Chen, Shanhui Liao, Zhongliang Zhu, Jiahai Zhang, Chao Xu
Summary: C-degrons are critical for targeting receptor proteins of Cullin-RING E3 ligase complexes for protein degradation. FEM1 proteins, including FEM1B, recognize specific Arg/C-degrons such as CDK5R1 and SMCR8. The study reveals the binding mechanism between FEM1B and SMCR8, as well as the role of CRL2(FEM1B) in regulating the lifespan of SMCR8.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Virology
Lei Hou, Xiaohan Hu, Jinshuo Guo, Rong Quan, Li Wei, Jing Wang, Jiangwei Song, Jue Liu
Summary: In this study, we found that aMPV/C infection induces MAVS degradation through the ubiquitin-proteasome pathway at the post-translational level. Furthermore, specific amino acid sites were identified as pivotal in the formation of polyubiquitin chains during MAVS degradation. E3 ubiquitin ligases targeting MAVS were also identified, with RNF5 shown to be involved in MAVS degradation in aMPV/C-infected cells.
Article
Oncology
Liangzi Cao, Hao Li, Xiaofang Liu, Yubang Wang, Bowen Zheng, Chengzhong Xing, Naijin Zhang, Jingwei Liu
Summary: This study investigated the molecular alterations and clinical relevance of NEDD4 E3 ligase family genes in 33 cancer types. The findings revealed increased expression of NEDD4 members in pancreatic cancer and decreased expression in thyroid cancer. NEDD4 family genes exhibited a range of mutation frequencies, with HECW1 and HECW2 showing relatively high mutation rates. Breast cancer presented with a high frequency of NEDD4 copy number amplification. Additionally, NEDD4 family members were involved in various pathways such as p53, Akt, apoptosis, and autophagy, and their expression correlated with the survival of cancer patients.
Article
Medicine, General & Internal
Zhonghui Zhang, Liang Zhong, Wenran Dan, Xuan Chu, Chen Liu, Xu Luo, Peng Wan, Zhenyan Liu, Yang Lu, Xiao Wang, Beizhong Liu
Summary: The research reveals that ZFP91 enhances AML cell proliferation and inhibits apoptosis by interacting with RIP1 and inhibiting its K48-linked ubiquitination. These findings provide evidence that targeted inhibition of ZFP91 could be a potential approach to treat AML.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Guangqiang Zhang, Junjiao Yang, Meng Zhang, Qinxue Li, Yunzhen Wu, Xiaoyu Zhao, Huifei Zhang, Yong Wang, Jiajie Wu, Wei Wang
Summary: The study revealed that TaPUB1 enhances the resistance of wheat to Cd stress by modulating the stability of TaIRT1 and TaIAA17 proteins in three ways. TaPUB1 overexpression lines showed reduced Cd accumulation and ROS accumulation, with inhibitory effects on Cd uptake.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Wen Li, Reham M. Elhassan, Xuben Hou, Hao Fang
Summary: The PROTAC technology, based on the ubiquitin-proteasome system, has become a potential strategy for targeted cancer therapy. It has advantages over traditional small-molecule inhibitors in terms of pharmacodynamics, selectivity, and drug resistance. Several small molecule PROTACs are currently in Phase I clinical trial, showing promising advances in targeted anticancer research.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Me-Sun Kim, Seo-Rin Ko, Yu Jin Jung, Kwon-Kyoo Kang, Yung-Jo Lee, Yong-Gu Cho
Summary: Plants produce stress-resistant substances in response to abiotic stress, and a protein conversion mechanism helps break down damaged proteins. The study focused on the function of OsPUB7 gene in Oryza sativa and found that OsPUB7 plays a role in drought and salinity stress resistance. The interaction between OsPUB7 and other stress-related genes suggests its potential as a target for breeding and future research on drought tolerance in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Sunil Singh, Joel Ng, J. Sivaraman
Summary: The HECT E3 ligase family consists of three sub-families: NEDD4, HERC, and Other, with the latter being less explored. Members of the Other subfamily have diverse N-terminal regions preceding the conserved HECT domains, which influence the stability and activity of the proteins. They assemble atypical ubiquitin linkages and cooperate with E3 ligases from other subfamilies to form branched ubiquitin chains on substrates, making them potential candidates for therapeutic drug development.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Microbiology
Kelong Ma, Rundong Shu, Hongtao Liu, Jiaqi Fu, Zhao-Qing Luo, Jiazhang Qiu
Summary: Legionella pneumophila, a pathogenic bacteria, can create an environment conducive to its replication by exploiting host cell signaling pathways and the ubiquitin system. In this study, we identified a novel effector protein, Lug15, which functions as an E3 ligase and remodels the Legionella phagosome.
Article
Biochemistry & Molecular Biology
Viktoria K. Ilic, Olga Egorova, Ernest Tsang, Milena Gatto, Yi Wen, Yong Zhao, Yi Sheng
Summary: The proto-oncogene MDM2 is frequently amplified in many human cancers and its overexpression is associated with poor prognosis. MDM2 shows oncogenic activity by negatively regulating tumor suppressor p53 and proteins involved in DNA repair, cell cycle control, and apoptosis pathways. Inhibition of MDM2 activity has been pursued as an attractive direction for anti-cancer therapeutics. This study identified a biflavonoid compound Hinokiflavone as a promising candidate compound targeting MDM2. Hinokiflavone was shown to bind the MDM2-MDMX RING domain and inhibit MDM2-mediated ubiquitination in vitro. Hinokiflavone treatment downregulated MDM2 and MDMX and induced apoptosis in various cancer cell lines. Hinokiflavone demonstrated tumor-suppressive activity that is both p53-dependent and -independent.
Review
Pharmacology & Pharmacy
Juhee Ryu, Youngkeun Ahn, Hyun Kook, Young-Kook Kim
Summary: This article reviews the role and therapeutic implications of non-coding RNAs (ncRNAs) in vascular calcification (VC). Studies suggest that ncRNAs, including miRNAs, lncRNAs, and circRNAs, play important roles in regulating VC and may be useful in clinical diagnosis and treatment.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Medicine, Research & Experimental
Nakwon Choe, Duk-Hwa Kwon, Juhee Ryu, Sera Shin, Hye Jung Cho, Hosouk Joung, Gwang Hyeon Eom, Youngkeun Ahn, Woo Jin Park, Kwang-Il Nam, Young-Kook Kim, Hyun Kook
MOLECULAR THERAPY-NUCLEIC ACIDS
(2020)
Article
Medicine, Research & Experimental
Liyan Bai, Hae Jin Kee, Sin Young Choi, Young Mi Seok, Gwi Ran Kim, Seung-Jung Kee, Hyun Kook, Myung Ho Jeong
Summary: The genetic deletion of HDAC5 and treatment with class IIa HDAC inhibitors can prevent increases in blood pressure and arterial wall thickness induced by Ang II. HDAC5 plays a role in vascular contraction and hypertrophy, and its inhibition may be a potential target for hypertension treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Cardiac & Cardiovascular Systems
Somy Yoon, Mira Kim, Hangyeol Lee, Gaeun Kang, Kenneth Bedi, Kenneth B. Margulies, Rajan Jain, Kwang-Il Nam, Hyun Kook, Gwang Hyeon Eom
Summary: The study reveals that neuronal nitric oxide synthase induces diastolic dysfunction by S-nitrosylation of HDAC2, suggesting a new therapeutic platform for refractory heart failure with preserved ejection fraction.
Article
Biochemistry & Molecular Biology
Duk-Hwa Kwon, Joo-Young Kang, Hosouk Joung, Ji-Young Kim, Anna Jeong, Hyun-Ki Min, Sera Shin, Yun-Gyeong Lee, Young-Kook Kim, Sang-Beom Seo, Hyun Kook
Summary: The balance between KDM2B and SET7, two competing enzymes that respectively inhibit and activate the same gene-regulating protein SRF, plays a critical role in skeletal muscle cell differentiation. KDM2B inhibits cell differentiation by demethylating SRF, while SET7 activates differentiation by methylating SRF. This study reveals SRF as a novel target of KDM2B and provides insights into skeletal muscle development in health and disease.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jung Ha Kim, Kabsun Kim, Inyoung Kim, Semun Seong, Hyun Kook, Kyung Keun Kim, Jeong-Tae Koh, Nacksung Kim
Summary: CrkL regulates the coupling signals between osteoblasts and osteoclasts by decreasing RANKL expression, which plays a crucial role in bone homeostasis by inhibiting both osteoblast-dependent osteoclast differentiation and osteoclast-dependent osteoblast differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yongwoon Lim, Anna Jeong, Duk-Hwa Kwon, Yeong-Un Lee, Young-Kook Kim, Youngkeun Ahn, Taewon Kook, Woo-Jin Park, Hyun Kook
Summary: Various heart diseases lead to ineffective cardiac contraction, with cardiac fibrosis being a contributing factor. PCAF plays a key role in the activation of cardiac fibroblasts during cardiac remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Duk-Hwa Kwon, Nakwon Choe, Sera Shin, Juhee Ryu, Nacksung Kim, Gwang Hyeon Eom, Kwang-Il Nam, Hyung Seok Kim, Youngkeun Ahn, Young-Kook Kim, Woo Jin Park, Susan M. Mendrysa, Hyun Kook
Summary: The study revealed the driver and activation pathway of vascular calcification, causing severe complications in patients with cardiovascular and renal diseases, potentially providing valuable information for novel therapeutic strategies targeting related conditions.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Surgery
Dong Hoon Lee, Young-Suk Cho, Wook Hyun Jung, Sang Chul Lim, Kwang Il Nam
Summary: The study conducted an anatomical analysis of the anterior jugular vein in Koreans, finding that a safety zone at the main landmark of the neck could be used to prevent damage to the AJV.
JOURNAL OF CRANIOFACIAL SURGERY
(2022)
Article
Anatomy & Morphology
Hyeng-Kyu Park, Jae-Young Han, Young-Suk Cho, Kwang-Il Nam
Summary: The tensor of the vastus intermedius (TVI) originated from the anteroinferior greater trochanter and inserts into the upper patella. It has been overlooked but warrants more research due to the development of micro-surgery and detailed rehabilitation treatments.
Article
Respiratory System
Chung Man Sung, Hong Chan Kim, Jisun Kim, Jae Gu Kim, Sang Chul Lim, Min-Ho Shin, Kwangil Nam, Jongho Lee, Daniel Vena, David P. White, Hyung Chae Yang, Andrew Wellman
Summary: This study analyzed adherence to autotitrating positive airway pressure (APAP) treatment in patients with epiglottic collapse. It found that patients with epiglottic collapse had a higher APAP adherence failure rate and lower usage time compared to patients without epiglottic collapse. These patients also had a lower body mass index, which is an unfavorable predictor of APAP adherence.
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2022)
Article
Allergy
Hyung Chae Yang, Eun Jeong Won, Moon-Ju Kim, Chung Man Sung, Joon Haeng Rhee, Kwang Il Nam
Summary: The intralymphatic injection of Metagonimus yokogawai extract protein (My-TP) was shown to reduce allergic symptoms and eosinophil infiltration in the nasal mucosa of OVA-sensitized allergic rhinitis mice. Additionally, My-TP treatment group exhibited significantly decreased levels of OVA-specific IgE and white blood cell counts in nasal lavage. This study suggests a potential therapeutic effect of My-TP in allergic rhinitis by modulating the Treg/Th2 balance.
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Hyunjong Kim, Chung Man Sung, Hunpyo Ju, Hyeongoh Park, Jinmo Jeong, Kwang Il Nam, Hyung Chae Yang, Jongho Lee
ADVANCED MATERIALS TECHNOLOGIES
(2020)
Meeting Abstract
Allergy
Hyung Chae Yang, Eun Jeong Won, Chungman Sung, Sang Chul Lim, Kwang Il Nam
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2020)
