Article
Biochemistry & Molecular Biology
Ninon Very, Stephan Hardiville, Amelie Decourcelle, Julien Thevenet, Madjid Djouina, Adeline Page, Gerard Vergoten, Celine Schulz, Julie Kerr-Conte, Tony Lefebvre, Vanessa Dehennaut, Ikram El Yazidi-Belkoura
Summary: Alteration of O-GlcNAcylation affects tumorigenesis and tumor progression, with a potential role in chemotherapy response. Combination treatment of 5-FU with Thiamet-G shows synergistic inhibitory effects on CRC progression by regulating TS and O-GlcNacylation levels. Overall, the findings suggest a new approach to enhance CRC response to 5-FU chemotherapy by targeting O-GlcNAcylation.
Article
Medicine, Research & Experimental
Elham Fakhr, Fatemeh Zare, Kayhan Azadmanesh, Ladan Teimoori-Toolabi
Summary: LEF1 plays an important role in the chemo-resistance of colorectal cancer cells, with downregulation of the long isoform making cells more sensitive to chemotherapy through reduced proliferation, increased apoptosis, or cell cycle arrest. Balancing the expression of long and short isoforms of LEF1 is crucial in overcoming chemo-resistance.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Oncology
Yaoqing Li, Chuchu Xu, Renjun Zhu, Liyijing Shen, Gengyuan Hu, Kelong Tao, Feng Tao, Zengxin Lu, Guolin Zhang
Summary: This study evaluates the value of tissue inhibitors of MMPs-2 (TIMP-2) as an indicator of 5-Fluorouracil (5-Fu) resistance in colorectal cancer. The results show that TIMP-2 expression is elevated in drug-resistant colorectal cancer cell lines and is closely related to 5-Fu resistance. Monitoring serum TIMP-2 levels can help clinicians identify 5-Fu resistance in colorectal cancer patients earlier during chemotherapy.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Pathology
Yu-Ling Qu, Xiao-Li Liu, Shan-Yu Zhao, Xue-Feng Zhai
Summary: This study found that SPOCK1 is upregulated in 5-FU-resistant CRC cells and that knockdown of SPOCK1 increases their sensitivity to 5-FU. Conversely, overexpression of SPOCK1 enhances CRC cell resistance to 5-FU and reduces cell apoptosis.
PATHOLOGY RESEARCH AND PRACTICE
(2022)
Article
Oncology
Min Li, Mingyue Xia, Ziyu Zhang, Yanyin Tan, Enjie Li, Zhigang Guo, Mingzhi Fang, Yong Zhu, Zhigang Hu
Summary: This study reveals the important role of the METTL3/RAD51AP1 axis in the acquisition of 5-FU resistance in CRC, and targeting METTL3/RAD51AP1 may be a promising adjuvant therapeutic strategy for patients with CRC, particularly for those with 5-FU-resistant CRC.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Biology
Marlies C. Ludikhuize, Sira Gevers, Nguyen T. B. Nguyen, Maaike Meerlo, S. Khadijeh Shafiei Roudbari, M. Can Gulersonmez, Edwin C. A. Stigter, Jarno Drost, Hans Clevers, Boudewijn M. T. Burgering, Maria J. Rodriguez Colman
Summary: In p53-deficient colorectal cancer organoids, 5-fluorouracil induces pyrimidine imbalance, which leads to DNA damage and cell death. Rewiring glucose metabolism through PDK inhibition by DCA enhances 5-FU toxicity in glycolytic p53-deficient organoids. This has important implications for improving chemotherapy strategies for colorectal cancer, and p53 emerges as a key factor in determining the response to 5-FU.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tsui-Chin Huang, Kuan-Chieh Peng, Tzu-Ting Kuo, Li-Chun Lin, Bai-Chia Liu, Shu-Ping Ye, Chien-Chou Chu, Shih-Min Hsia, Hsin-Yi Chang
Summary: By using pharmacogenomic analysis, this study found a correlation between APC gene mutations in colorectal cancer cells and resistance, showing synergistic effects of combining a MEK inhibitor with 5-FU on cells with APC truncations. Additionally, targeting ROCK was identified as a potential avenue to restore 5-FU response in resistant cells with wild-type APC background.
Article
Biochemistry & Molecular Biology
Haili Wu, Jin'e Du, Chenglu Li, Hanqing Li, Huiqin Guo, Zhuoyu Li
Summary: Resistance to 5-Fluorouracil (5-Fu) chemotherapy is a major obstacle in colon cancer treatment. This study found that the dietary flavonoid kaempferol can reverse the drug resistance of colorectal cancer cells to 5-Fu, suggesting a potential treatment option. The mechanism behind this reversal involves the regulation of the miR-326-hnRNPA1/A2/PTBP1-PKM2 axis and inhibition of glycolysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Guolin Zhang, Xin Luo, Zian Wang, Jianbin Xu, Wei Zhang, Engeng Chen, Qing Meng, Di Wang, Xuefeng Huang, Wei Zhou, Zhangfa Song
Summary: The study revealed that TIMP-2 is highly expressed in 5-Fu resistant colorectal cancer patients and its elevated levels contribute to drug resistance by activating the ERK/MAPK signaling pathway.
Article
Cell Biology
Sabrina Blondy, Hugo Talbot, Sofiane Saada, Niki Christou, Serge Battu, Julie Pannequin, Marie-Odile Jauberteau, Fabrice Lalloue, Mireille Verdier, Muriel Mathonnet, Aurelie Perraud
Summary: Colorectal cancer (CRC) is the third most common cancer worldwide, and resistance to 5-fluorouracil (5-FU) can lead to poor clinical outcomes. Sortilin overexpression has been identified as a potential biomarker of 5-FU resistance in CRC, with higher levels correlating with tumor grades. This new prognostic factor may be used to combat CRC aggressiveness.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Oncology
Jingui Wang, Xiaoqian Zhang, Junling Zhang, Shangwen Chen, Jing Zhu, Xin Wang
Summary: The study revealed that high expression of CRART16 promotes resistance to 5-FU in CRC cells by suppressing miR-193b-5p, leading to the modulation of HMGA2 expression and activation of the MAPK signaling pathway.
CANCER CELL INTERNATIONAL
(2021)
Article
Chemistry, Medicinal
Dongdong Luo, Xiaochun Liu, Leilei Jiang, Zhikun Guo, Yan Lv, Xiaochen Tian, Xiaoyan Wang, Shuxiang Cui, Shengbiao Wan, Xianjun Qu, Ximing Xu, Xiaoyang Li
Summary: In this study, 38 novel S1PR2 antagonists were synthesized and their structure-activity relationship investigated. Compound 43 showed the strongest interaction with S1PR2 and exhibited potent 5-FU resensitizing activity in 5-FU-resistant tumor cells.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Oncology
Adarsh Kumar, Ankit Kumar Singh, Harshwardhan Singh, Suresh Thareja, Pradeep Kumar
Summary: Thymidylate synthase is a crucial enzyme for DNA synthesis, and its overexpression leads to drug resistance in cancer cells. This review summarizes different approaches for regulating thymidylate synthase to overcome resistance in colorectal cancer.
Article
Oncology
Lu Chen, Kangyue Sun, Wenjie Qin, Bing Huang, Changjie Wu, Junsheng Chen, Qiuhua Lai, Xinke Wang, Rui Zhou, Aimin Li, Side Liu, Yue Zhang
Summary: Elevated expression of LIMK1 in colorectal cancer cells promotes cell proliferation and increased resistance to 5-FU. Downregulation of YTH domain-containing 2 (YTHDC2) in CRC cells leads to increased stability and expression of LIMK1 mRNA. Overexpression of LIMK1 induces eIF2 alpha phosphorylation, triggering endoplasmic reticulum stress and promoting stress granule formation, ultimately resulting in 5-FU resistance.
Article
Medicine, Research & Experimental
Zhize Qiu, Shiqi Qiu, Wenli Mao, Wu Lin, Qiqi Peng, Hao Chang
Summary: This study explored the role of LOXL2 in 5-FU resistance of colorectal cancer (CRC) by analyzing mRNA and protein levels of LOXL2 in public databases, validating it in clinical tissues, and detecting it in 5-FU treated cell lines. Elevated LOXL2 expression was associated with poor prognosis in CRC patients, and it promoted resistance to 5-FU, increased proliferation, and inhibited 5-FU-induced apoptosis in CRC cells. LOXL2 activated the Hedgehog signaling pathway, leading to the upregulation of the anti-apoptotic gene BCL2 in CRC cells. The Hedgehog signaling pathway inhibitor cyclopamine blocked the upregulation of BCL2 mediated by LOXL2. This study demonstrates that LOXL2 reduces 5-FU sensitivity through the Hedgehog/BCL2 signaling pathway in CRC.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2023)
Article
Gastroenterology & Hepatology
Jia-Yin Yao, Yi Jiang, Jia Ke, Yi Lu, Jun Hu, Min Zhi
WORLD JOURNAL OF GASTROENTEROLOGY
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Sijing Cheng, Jun Hu, Xianrui Wu, Na Jiao, Yichen Li, Yibo Huang, Ruixin Zhu, Min Zhi, Lixin Zhu, Ping Lan
Article
Biochemistry & Molecular Biology
Jing Cai, Wenfeng Liu, Chun Wa Wong, Wenbo Zhu, Yuan Lin, Jun Hu, Wencang Xu, Jifu Zhang, Max Sander, Zhuo Wang, Jia Dan, Jiayu Zhang, Yang Liu, Li Guo, Zhen Qin, Xincheng Liu, Ying Liu, Guangmei Yan, Sihan Wu, Jiankai Liang
Article
Oncology
Jing Cai, Kaiying Lin, Wei Cai, Yuan Lin, Xincheng Liu, Li Guo, Jifu Zhang, Wencang Xu, Ziqing Lin, Chun Wa Wong, Max Sander, Jun Hu, Guangmei Yan, Wenbo Zhu, Jiankai Liang
MOLECULAR ONCOLOGY
(2020)
Article
Gastroenterology & Hepatology
Jiayin Yao, Yi Jiang, Jia Ke, Yi Lu, Jun Hu, Min Zhi
Summary: The study developed a predictive model that effectively predicted early-onset complications leading to surgery and created a nomogram for clinical decision-making. Results showed that age, disease duration, perianal disease, previous surgery history, and extraintestinal manifestations were independent predictors of early-onset complications.
DISEASES OF THE COLON & RECTUM
(2021)
Article
Virology
Jia Dan, Lin Nie, Xudong Jia, Cuiying Xu, Jing Cai, Yuan Lin, Jun Hu, Wenbo Zhu, Yinyin Li, Dong Chen, Ying Liu, Cheng Hu, Guangmei Yan, Jiankai Liang, Qinfen Zhang
Summary: This study investigated the infection and replication process of oncolytic alphavirus M1 in HepG2 liver cancer cells using transmission electron microscopy. They found that M1 virus induced vacuolization and apoptosis in cancer cells, with the endoplasmic reticulum participating in nucleocapsid assembly. Special cytopathic vacuoles formed during infection appeared to be involved in virus replication, maturation, and release. The findings provide a structural basis for understanding M1 virus-host interactions in tumor cells.
Article
Biochemistry & Molecular Biology
Sijing Cheng, Jun Hu, Xianrui Wu, Ji-An Pan, Na Jiao, Yichen Li, Yibo Huang, Xutao Lin, Yifeng Zou, Yuan Chen, Lixin Zhu, Min Zhi, Ping Lan
Summary: The study shows that FUT2 loss-of-function mutation alters the gut microbiota composition in IBD patients, reducing adherent bacteria while increasing butyrate-producing bacteria, leading to intestinal inflammation.
JOURNAL OF GENETICS AND GENOMICS
(2021)
Article
Biochemistry & Molecular Biology
Jiayu Zhang, Ying Liu, Jingyi Tan, Yanming Zhang, Chun-Wa Wong, Ziqing Lin, Xincheng Liu, Max Sander, Xiaozhi Yan, Lebin Liang, Deli Song, Jia Dan, Yuwei Zhou, Jing Cai, Yuan Lin, Jiankai Liang, Jun Hu, Guangmei Yan, Wenbo Zhu
Summary: The study demonstrates that the oncolytic alphavirus M1 effectively kills both TNBC and non-TNBC, with different mechanisms of cell death observed in each subtype. It was found that necroptosis is activated in TNBC by the M1 virus, and the chemotherapy drug Doxorubicin can significantly enhance the oncolytic effect of the virus, especially in TNBC.
Correction
Virology
Jia Dan, Lin Nie, Xudong Jia, Cuiying Xu, Jing Cai, Yuan Lin, Jun Hu, Wenbo Zhu, Yinyin Li, Dong Chen, Ying Liu, Cheng Hu, Guangmei Yan, Jiankai Liang, Qinfen Zhang
Article
Biotechnology & Applied Microbiology
Yang Liu, Cuiying Xu, Xiaoting Xiao, Yinting Chen, Xiaobo Wang, Wenfeng Liu, Yaqian Tan, Wenbo Zhu, Jun Hu, Jiankai Liang, Guangmei Yan, Yuan Lin, Jing Cai
Summary: Oncolytic viruses (OVs) are promising anticancer immunotherapeutic agents, but their efficacy is limited by the immunosuppressive effect of tumor-associated myeloid cells (TAMCs). This study reveals that OVs induce TAMCs infiltration and strengthen their immunosuppressive phenotypes through the IL-6/PI3K-g/Akt pathway. Targeting PI3K-g can alleviate immunosuppression and enhance OVs' efficacy.
Article
Immunology
Jun Hu, Sijing Cheng, Jiayin Yao, Xutao Lin, Yichen Li, Wenxia Wang, Jingrong Weng, Yifeng Zou, Lixin Zhu, Min Zhi
Summary: This study examined the gut microbiota of patients with Crohn's disease (CD) and found that they had reduced levels of short chain fatty acid (SCFA) producing bacteria and increased levels of opportunistic pathogen Escherichia-Shigella. These differential genera were able to serve as diagnostic markers for CD and were correlated with changes in inflammation-related blood biochemical markers.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Xincheng Liu, Cui Guo, Tiandong Leng, Zhen Fan, Jialuo Mai, Jiehong Chen, Jinhai Xu, Qianyi Li, Bin Jiang, Ke Sai, Wenzhuo Yang, Jiayu Gu, Jingyi Wang, Shuxin Sun, Zhijie Chen, Yingqian Zhong, Xuanming Liang, Chaoxin Chen, Jing Cai, Yuan Lin, Jiankai Liang, Jun Hu, Guangmei Yan, Wenbo Zhu, Wei Yin
Summary: Differentiation therapy using small molecules is a promising strategy for improving the prognosis of glioblastoma (GBM). Histone acetylation plays an important role in cell fate determination, but whether histone acetylation in specific sites determines the fate of GBM cells remains to be explored.
CELL DEATH & DISEASE
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Jun Hu, Sijing Cheng, Jiayin Yao, Xutao Lin, Yichen Li, Wenxia Wang, Jingrong Weng, Yifeng Zou, Lixin Zhu, Min Zhi
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Deli Song, Xudong Jia, Xincheng Liu, Linyi Hu, Kaiying Lin, Tong Xiao, Yangyang Qiao, Jiayu Zhang, Jia Dan, Chunwa Wong, Cheng Hu, Ke Sai, Shoufang Gong, Max Sander, Runling Shen, Xiaoyu Chen, Xiaoting Xiao, Jiehong Chen, Yanming Zhang, Cailv Wei, Xiao Xiao, Jiankai Liang, Qinfen Zhang, Jun Hu, Wenbo Zhu, Guangmei Yan, Yuan Lin, Jing Cai
Summary: Oncolytic virus therapy has shown promising potential in tumor treatment, but not all patients benefit from it. In this study, MXRA8 was identified as a factor positively correlated with oncolysis induced by OVM, and it was found to promote the oncolytic efficacy of OVM. Furthermore, MXRA8 was shown to directly bind to OVM, acting as the entry receptor for the virus. The tumor selectivity of OVM was found to be determined by a combination of MXRA8 and the intracellular factor ZAP. This study provides a novel dual-biomarker for precision medicine in OVM therapy.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Oncology
Jing Cai, Wenbo Zhu, Yuan Lin, Jun Hu, Xincheng Liu, Wencang Xu, Ying Liu, Cheng Hu, Songmin He, Shoufang Gong, Guangmei Yan, Jiankai Liang
CANCER CELL INTERNATIONAL
(2020)