Article
Medicine, Research & Experimental
Zijian Chen, Zenghong Huang, Yanxin Luo, Qi Zou, Liangliang Bai, Guannan Tang, Xiaolin Wang, Guangwen Cao, Meijin Huang, Jun Xiang, Huichuan Yu
Summary: This study found that the NTRK3 gene promoter was more frequently methylated in CRC, leading to suppressed gene expression. It was confirmed that NTRK3 promoter methylation was associated with survival outcome in CRC and a nomogram predicting survival outcome was developed. Moreover, NTRK3 promoter methylation also predicted survival outcomes in other tumors.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Mengsi Yu, Changcheng Yang, Song Wang, Ying Shi, Jialu Wang, Cunren Meng, Li Xue, Zhaoyun Chen
Summary: This study aimed to investigate the role of plasma mSEPT9 in predicting LM in CRC patients. The levels of plasma mSEPT9 and CEA were significantly increased in CRC patients with LM. Multivariate logistic regression analysis showed that plasma mSEPT9 was an independent risk factor for predicting LM in CRC. ROC curves showed that mSEPT9 and CEA could efficiently distinguish LM from NM in CRC.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Oncology
Philipp Krausewitz, Niklas Kluemper, Ayk-Peter Richter, Thomas Buettner, Glen Kristiansen, Manuel Ritter, Joerg Ellinger
Summary: This study found that SEPT9 and SHOX2 were highly methylated in PCa tissue and could discriminate disease status, tumor stage, and grade. In ccfDNA, the methylation of these two biomarkers allowed discrimination of localized and metastatic disease and increased shortly after prostate biopsy only in PCa patients. Therefore, mSEPT9 and mSHOX2 are promising non-invasive measures for PCa evaluation.
Article
Medicine, General & Internal
Luyao Zhao, Muran Li, Shiwu Zhang, Yandi Liu
Summary: This study evaluated the potential performance of plasma SEPT9 methylation as a noninvasive biomarker for the diagnosis of gastric cancer. The results suggest that mSEPT9 may serve as a useful and noninvasive biomarker for the diagnosis of gastric cancer.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Rashidah Baharudin, Nurul Qistina Rus Bakarurraini, Imilia Ismail, Learn-Han Lee, Nurul Syakima Ab Mutalib
Summary: Colorectal cancer is a major cause of cancer-related deaths worldwide, and microRNAs play a significant role in its occurrence and progression. However, the methylation profiles of microRNAs in colorectal cancer patients are still unknown. Methylation is an important factor in cancer detection, and this review aims to provide insight into the current understanding of microRNA methylation in colorectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Adhara Brandao Lima, Mariana Bisarro dos Reis, Marcus Matsushita, Monise Tadin dos Reis, Marco Antonio de Oliveira, Rui Manuel Reis, Denise Peixoto Guimaraes
Summary: This study conducted plasma methylation analysis in a Brazilian population and found that a combination of SEPT9 and BMP3 genes had the highest performance in detecting colorectal cancer.
Article
Medicine, Research & Experimental
Hui Zhang, Chenxin Xu, Chen Shi, Junying Zhang, Ting Qian, Zhuo Wang, Rong Ma, Jianzhong Wu, Feng Jiang, Jifeng Feng
Summary: In this study, HPSE2 was identified as a novel tumor suppressor gene frequently inactivated through promoter methylation in CRC, with its hypermethylation-low expression associated with poor patient prognosis. HPSE2 exerts its tumor suppressive function by activating the p53/p21 signaling cascade to inhibit tumor growth. Promoter hypermethylation of HPSE2 represents a potential therapeutic target in patients with CRC, especially those with late-stage disease.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Yunfeng Zhang, Qian Wu, Linhao Xu, Hong Wang, Xin Liu, Sihui Li, Tianliang Hu, Yanying Liu, Quanzhou Peng, Zhiwei Chen, Xianrui Wu, Jian-Bing Fan
Summary: Colorectal cancer is a major cause of cancer-related deaths worldwide. A new qPCR-based assay combining the detection of the DNA methylation status of ten candidate genes showed promising results in detecting CRC patients. A 4-marker methylation model including two novel markers (kcnj12 and znf132) provided a non-invasive option for CRC screening with high performance.
CLINICAL EPIGENETICS
(2021)
Article
Oncology
Qin Xiang, Dishu Zhou, Xinni Xiang, Xin Le, Chaoqun Deng, Ran Sun, Chunhong Li, Huayang Pang, Jin He, Zeze Zheng, Jun Tang, Weiyan Peng, Xi Peng, Xiaoqian He, Fan Wu, Jingfu Qiu, Yongzhu Xu, Tingxiu Xiang
Summary: The incidence of colorectal cancer (CRC) has increased in recent years. Identifying accurate methylation biomarkers, such as neuroglobin (NGB), could improve the efficacy of CRC treatment. NGB is downregulated or silenced in most CRC tissues and cell lines, and its hypermethylation is detected in tumor tissue but not in normal tissues. Overexpression of NGB inhibits CRC tumor growth and angiogenesis through GPR35.
CLINICAL EPIGENETICS
(2023)
Review
Oncology
Nayeralsadat Fatemi, Sascha Tierling, Hamidreza Aboulkheyr Es, Maryam Varkiani, Ehsan Nazemalhosseini Mojarad, Hamid Asadzadeh Aghdaei, Joern Walter, Mehdi Totonchi
Summary: Colorectal cancer, the second leading cause of cancer death worldwide, is closely associated with epigenetic changes such as DNA methylation. Studies have shown that DNA methylation markers have high accuracy and reproducibility in noninvasive biosamples, making them promising for early diagnosis and personalized treatment of CRC. Further research and development of methylation biomarkers and detection methods are crucial for CRC diagnosis.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Medical Laboratory Technology
Irina V. Botezatu, Valentina N. Kondratova, Anna M. Stroganova, Svetlana L. Dranko, Anatoly V. Lichtenstein
Summary: This study presents a new PCR method, qDMA-HP, for quantitative analysis of methylated DNA in liquid biopsy without the need for data normalization. The method was successfully tested for colorectal cancer liquid biopsy and showed promising results.
CLINICA CHIMICA ACTA
(2023)
Article
Oncology
Huihui Ji, Kehan Li, Wenxiao Jiang, Jingwei Li, Jian-An Zhang, Xueqiong Zhu
Summary: This study identified abnormally methylated tumor suppressor genes associated with cervical cancer. The low expression of MRVI1 and NTRK3 genes was associated with poor overall survival in cervical cancer patients. The promoter methylation levels of MRVI1 and NTRK3 could discriminate cervical cancer from healthy samples.
FRONTIERS IN ONCOLOGY
(2022)
Article
Genetics & Heredity
Lei Zhang, Dapeng Li, Lijing Gao, Jinming Fu, Simin Sun, Hao Huang, Ding Zhang, Chenyang Jia, Ting Zheng, Binbin Cui, Yanlong Liu, Yashuang Zhao
Summary: Early and specific detection of cancer is crucial for appropriate treatment. This study reveals that QKI methylation can serve as a diagnostic marker for colorectal cancer. By integrating methylation data from various cancer types, the researchers demonstrate the specific and high diagnostic ability of QKI promoter methylation in colorectal cancer.
FRONTIERS IN GENETICS
(2022)
Article
Oncology
Tasmina Ferdous Susmi, Atikur Rahman, Md. Moshiur Rahman Khan, Farzana Yasmin, Md. Shariful Islam, Omaima Nasif, Sulaiman Ali Alharbi, Gaber El-Saber Batiha, Mohammad Uzzal Hossain
Summary: The study found that PDE9A was significantly down-regulated in CRC tissues compared to normal tissues, and methylation in the DNA promoter region could also affect PDE9A gene expression. High expression of PDE9A gene was associated with higher survival rates in CRC patients. PDE9A showed strong correlation with rectal cancer recurrence and had interactions with other genes to drive survival pathways in CRC cells. Overall, PDE9A could serve as a potential tumor biomarker for CRC.
Article
Oncology
Weihua Li, Lei Guo, Wanxiangfu Tang, Yutong Ma, Xiaonan Wang, Yang Shao, Hong Zhao, Jianming Ying
Summary: This study aimed to construct a model for predicting liver metastasis risk in CRC patients by utilizing differential methylation signals in primary tumors. A total of 241,573 DMRs were identified, with 182 hypermethylated DMRs associated with metastasis in stage II CRC patients. The predictive model showed promising performance with an AUC of 0.701 and was able to distinguish metastases in different sites with an AUC of 0.933. Identified DMRs in the promoter region of known prognostic biomarkers suggest their potential role in predicting liver metastasis in early-stage CRC patients.
CLINICAL EPIGENETICS
(2021)
Article
Biochemistry & Molecular Biology
Maryam Enayatkhani, Mehdi Hasaniazad, Sobhan Faezi, Hamed Guklani, Parivash Davoodian, Nahid Ahmadi, Mohammad Ali Einakian, Afsaneh Karmostaji, Khadijeh Ahmadi
Summary: This study utilized bioinformatics approaches to design a novel multi-epitope vaccine against the novel Coronavirus, potentially triggering multiple immune responses. Computational tools were used to validate the biological activities of the vaccine, predict the binding of the protein candidate with Toll-like receptor 4 and HLA-A*11:01, and molecular dynamics simulation was employed to evaluate the stability of the designed fusion protein with the receptors.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Oncology
Rosa Jahangiri, Fatemeh Mosaffa, Amirnader Emami Razavi, Ladan Teimoori-Toolabi, Khadijeh Jamialahmadi
Summary: This study found that aberrant promoter methylation of PAX2 and overexpression of AIB1 are associated with tamoxifen response in breast carcinoma patients, which may serve as predictive or prognostic biomarkers. Further research is needed to explore the potential of using PAX2 and AIB1 expression and their methylation-mediated regulation as a new target therapy for better disease management.
JOURNAL OF ONCOLOGY PHARMACY PRACTICE
(2022)
Article
Oncology
Ali Khaligh, Mohammad Sadegh Fazeli, Habibollah Mahmoodzadeh, Amirhosein Mehrtash, Setareh Kompanian, Sirous Zeinali, Ladan Teimoori-Toolabi
Summary: The study found that using 14 genetic markers for detection significantly increased the detection rate of MSI+ cases compared to using the pentaplex panel, with DCD, MT1X, and DNMT3a showing the best diagnostic value among them. These markers help to better identify late-stage MSI+ tumors.
Article
Pharmacology & Pharmacy
Fatemeh Gheidari, Ehsan Arefian, Fatemeh Jamshidi Adegani, Mohammad Reza Kalhori, Ehsan Seyedjafari, Mahboubeh Kabiri, Ladan Teimoori-Toolabi, Masoud Soleimani
Summary: miR-424 acts as a tumor suppressor miRNA in glioblastoma cells by significantly inhibiting cell proliferation and migration, promoting apoptosis and cell-cycle arrest. It exerts its effects by regulating multiple target genes from the ERBB pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Elham Fakhr, Fatemeh Zare, Kayhan Azadmanesh, Ladan Teimoori-Toolabi
Summary: LEF1 plays an important role in the chemo-resistance of colorectal cancer cells, with downregulation of the long isoform making cells more sensitive to chemotherapy through reduced proliferation, increased apoptosis, or cell cycle arrest. Balancing the expression of long and short isoforms of LEF1 is crucial in overcoming chemo-resistance.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Oncology
Maryam Alizadeh-Sedigh, Mohammad Sadegh Fazeli, Habibollah Mahmoodzadeh, Shahin Behrouz Sharif, Ladan Teimoori-Toolabi
Summary: This study suggests that investigating the methylation levels of specific promoter regions in plasma and tumor tissues can serve as a biomarker for early detection of colorectal cancer. The methylation levels of specific promoter regions, such as FBN1, SPG20, and SEPT9, show good diagnostic value in plasma.
Article
Biochemistry & Molecular Biology
Behzad Shahbazi, Seyed Shahriar Arab, Ladan Mafakher, Kayhan Azadmansh, Ladan Teimoori-Toolabi
Summary: This study aimed to investigate the structural basis of the interaction between PEDF and its receptors using bioinformatics approaches, in order to identify the critical amino acids for designing anticancer peptides. The results showed that PEDF could effectively interact with different receptors and its N-terminal played an important role in the interaction with receptors.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Behzad Shahbazi, Ladan Mafakher, Ladan Teimoori-Toolabi
Summary: This study identified two potential drug candidates, Luteolin and Chrysin, using computational approaches, for combating COVID-19. Experimental results showed that Luteolin and Chrysin effectively interact with ACE2 and can block the binding of SARS-CoV-2 to ACE2, suggesting their potential as inhibitors against early stage infection and transmission of the virus.
JOURNAL OF MOLECULAR MODELING
(2022)
Article
Biochemical Research Methods
Maryam Alizadeh-Sedigh, Habibollah Mahmoodzadeh, Mohammad Sadegh Fazeli, Mohammad Haddadi-Aghdam, Ladan Teimoori-Toolabi
Summary: This study investigated several gene mutations in colorectal cancer tumor tissue and evaluated their diagnostic performance in plasma cfDNA. The results showed that a panel of PIK3CA, KRAS, and BRAF mutations had good diagnostic performance for detecting colorectal cancer in plasma cfDNA.
MOLECULAR AND CELLULAR PROBES
(2022)
Article
Biochemical Research Methods
Mohammad Shoushtari, Ladan Mafakher, Saman Rahmati, Mostafa Salehi-Vaziri, Arash Arashkia, Farzin Roohvand, Ladan Teimoori-Toolabi, Kayhan Azadmanesh
Summary: This study aimed to design vaccine candidates for Dengue virus by investigating the bc loop, EDIII, and NS1 regions, and validating the structures and antigenicity of these vaccines. The results showed that the designed vaccines could effectively induce humoral immunity and have high affinity with specific antibodies.
MOLECULAR AND CELLULAR PROBES
(2022)
Article
Biochemical Research Methods
Mansour Poorebrahim, Mohammad Foad Abazari, Leila Moradi, Behzad Shahbazi, Reza Mahmoudi, Hourieh Kalhor, Hassan Askari, Ladan Teimoori-Toolabi
Summary: K-Ras activating mutations are associated with tumor progression and metastasis. We aimed to target this mutated protein using small peptides and small molecules. Through computational methods, we selected peptides and small molecules that showed strong binding affinity to mutated and oncogenic K-Ras. These compounds hindered the growth of pancreatic tumor cells and reduced the expression of downstream genes. Our combinatorial approach introduces new potential candidates for blocking oncogenic K-Ras in various cancers. Further in vitro and in vivo analysis is needed to validate the efficacy and safety of these compounds.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Masoumeh Kiani-Zadeh, Mohammad Reza Rezvany, Soodeh Namjoo, Mahmood Barati, Mohammad Hossein Mohammadi, Bahare Ghasemi, Tahere Tabatabaei, Ardeshir Ghavamzadeh, Farhad Zaker, Ladan Teimoori-Toolabi
Summary: This study aimed to investigate the expression of PTGS2 and VEGF-C, as well as the methylation status of PTGS2, in AML patients. The results showed elevated levels of VEGF-C and PTGS2, which correlated with WBC, Platelet, and Hemoglobin levels. Overexpression of VEGF-C and PTGS2 was associated with poorer survival in patients.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Fatemeh Gheidari, Ehsan Arefian, Fatemeh Saadatpour, Mahboubeh Kabiri, Ehsan Seyedjafari, Ladan Teimoori-Toolabi, Masoud Soleimani
Summary: In this study, we found that miR-429 effectively suppressed the ERBB genetic signaling pathway and inhibited cell proliferation, migration, and induced cell-cycle arrest and apoptosis in GBM. As such, miR-429 has potential as a therapeutic option for GBM.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Public, Environmental & Occupational Health
Sara Sheikholeslami, Fereidoun Azizi, Asghar Ghasemi, Abbas Alibakhshi, Hossein Parsa, Setareh Shivaee, Marjan Zarif-Yeganeh, Mehdi Hedayati, Ladan Teimoori-Toolabi
Summary: This study investigated the expression and methylation of SLC5A8 in PTC and AING. It was found that the level of SLC5A8 was significantly downregulated in PTC tissues. Aberrant promoter hyper methylation of SLC5A8 was related to aggressive PTC.
IRANIAN JOURNAL OF PUBLIC HEALTH
(2022)
Review
Public, Environmental & Occupational Health
Sara Sheikholeslami, Marjan Zarif-Yeganeh, Samaneh Farashi, Fereidoun Azizi, Sima Kheradmand Kia, Ladan Teimoori-Toolabi, Mehdi Hedayati
Summary: This review investigates the impact of promoter hypermethylation on key tumor suppressor genes involved in cell growth and apoptosis in thyroid cancer. The aberrant epigenetic methylation of certain tumor suppressor genes may have potential clinical implications by activating kinase pathways, while other genes may suppress malignant cell proliferation by inhibiting the cell cycle and inducing apoptosis.
IRANIAN JOURNAL OF PUBLIC HEALTH
(2021)
