Article
Agriculture, Dairy & Animal Science
Andrea J. Lengi, Jacob W. Stewart, Melissa Makris, Michelle L. Rhoads, Benjamin A. Corl
Summary: This study found that heat stress affects the composition and viability of somatic cells in milk, which has implications for milk production and the immunocompetence of dairy cows.
Article
Biochemistry & Molecular Biology
Jing Fan, Zhihui Zhao, Haochen Wu, Xibi Fang, Fengshuai Miao, Xuanxu Chen, Xinyi Jiang, Jing Li, Ping Jiang, Haibin Yu
Summary: Transcriptome sequencing revealed differential expression of syndecan-3 (SDC3) in high-fat and low-fat mammary epithelial cells of Chinese Holstein cows. SDC3 has been implicated in inflammatory diseases and virus infections. This study investigated the effects of SDC3 on milk lipid metabolism and inflammation in bovine mammary epithelial cells (BMECs), and identified the AMPK/SIRT1 signaling pathway as the common regulatory pathway of SDC3. The findings showed that SDC3 co-regulates milk fat metabolism and inflammation through this pathway. These findings have important implications for breeding dairy cows based on multi-effect functional genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Baojun Yu, Jiamin Liu, Zhengyun Cai, Tong Mu, Di Zhang, Xiaofang Feng, Yaling Gu, Juan Zhang
Summary: MiR-19a plays a crucial role in regulating the proliferation and differentiation of bovine mammary epithelial cells (BMECs) and in biological processes related to triglyceride synthesis and lipid droplet formation by suppressing SYT1 expression.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Agriculture, Dairy & Animal Science
Jun Zhang, Lu Deng, Xia Zhang, Yangchun Cao, Mengmeng Li, Junhu Yao
Summary: Improving nitrogen utilization efficiency in dairy production systems is important for reducing feed costs and addressing environmental concerns. Supplementing a low protein content diet with essential amino acids (EAAs) has been widely used to enhance nitrogen efficiency. However, single EAA supplementation does not consider the complex interactions and responses among EAAs, leading researchers to explore the benefits of supplementing multiple EAAs. This review summarizes the literature supporting the advantages of complete EAA mixtures in improving milk protein yield (MPY) and highlights the mechanisms involved in mammary protein metabolism and milk protein synthesis (MPS).
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhiyun Hao, Jiqing Wang, Yuzhu Luo, Jiang Hu, Xiu Liu, Shaobin Li, Mingna Li, Bingang Shi, Liyan Hu, Yuan Liu, Huimin Zhen
Summary: miR-200c promotes the synthesis of triglycerides in ovine MECs by targeting PANK3, and its overexpression inhibits MECs proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Hui-Li Wang, Guang-Dong Xing, Yong Qian, Xue-Feng Sun, Ji-Feng Zhong, Kun-Lin Chen
Summary: The study showed that Dihydromyricetin (DMY) can protect dairy cow mammary epithelial cells against heat stress-induced injury by preventing oxidative stress and balancing mitochondrial fission and fusion. This finding provides evidence for DMY as a potential therapeutic drug for treating heat stress-induced mammary gland injury and mastitis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Review
Food Science & Technology
Lily Jaiswal, Mulumebet Worku
Summary: Cow's milk contains potential allergens in the form of proteins, making some infants, children, and adults allergic to cow's milk. Strict exclusion of milk and other dairy products puts children with cow's milk allergy at higher nutritional risk.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Agriculture, Dairy & Animal Science
Ming-Qi Wang, Chang-Hai Zhou, Shuai Cong, Dong-Xu Han, Chang-Jiang Wang, Yu Tian, Jia-Bao Zhang, Hao Jiang, Bao Yuan
Summary: The study demonstrates that LPS inhibits milk fat synthesis in MAC-T cells by upregulating miR-27a-3p, which targets the PPARG gene.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Veterinary Sciences
Wangsheng Zhao, Michael Adjei, Hongmei Wang, Yueling Yangliu, Jiangjiang Zhu, Huijuan Wu
Summary: ADIPOR1 plays a central role in regulating milk fat metabolism by influencing the transcription of genes such as SREBF1, ACACA, FASN, SCD, ATGL, and HSL in goat mammary gland. Overexpression and knockdown of ADIPOR1 resulted in significant changes in the expression levels of these genes, demonstrating its crucial role in the process. Additionally, treatment with AdipoRon led to a significant increase in the mRNA expression of most genes related to milk fat metabolism in GMECs.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Fangting Zhou, Jie Xue, Xi Shan, Lihua Qiu, Yongwang Miao
Summary: AGPAT6 plays a crucial role in milk fat synthesis in buffalo mammary epithelial cells (BuMECs), affecting the expression of key genes involved in fatty acid transport and activation, TG synthesis and transcription regulation, and fatty acid oxidation and TG degradation.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Veterinary Sciences
Kaiqiang Fu, Yuning Sun, Junbo Wang, Rongfeng Cao
Summary: The study demonstrated that tanshinone IIa has antioxidative effects on LPS-induced oxidative stress in dairy cow mammary epithelial cells, potentially serving as a therapeutic option for mastitis.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Xinlu Liu, Jinglin Shen, Jinxin Zong, Jiayi Liu, Yongcheng Jin
Summary: Beta-sitosterol, at concentrations ranging from 0.1 to 10 µM, has been shown to promote the expression of milk protein and fat synthesis-related genes and proteins in bovine mammary epithelial cells, suggesting its potential use as a feed additive to improve milk quality in dairy cows.
Article
Biochemistry & Molecular Biology
Zhiyun Hao, Yuzhu Luo, Jiqing Wang, Jon G. H. Hickford, Huitong Zhou, Jiang Hu, Xiu Liu, Shaobin Li, Jiyuan Shen, Na Ke, Weiwei Liang, Zhaochun Huang
Summary: The study demonstrated that miR-432 inhibits the proliferation of OMECs by targeting the genes SCD and LPL involved in milk fat synthesis. Transfection with miR-432 mimic reduced the expression of milk fat synthesis marker genes and triglyceride content in OMECs, while miR-432 inhibitor had the opposite effect. This study provides insights into the biological mechanisms of miR-432 in inhibiting milk fat synthesis in sheep.
Article
Agriculture, Dairy & Animal Science
Jie Song, Yao Hu, Lifang Wang, Changjin Ao
Summary: The study aimed to explore the potential of Artemisia annua ethanol extract (AAE) as a preventive agent for mastitis. The results showed that AAE pretreatment could alleviate inflammatory injury and tight junction abnormalities, which was associated with the inhibition of NF-kappa B signaling and the downregulation of CD36 levels.
Article
Agronomy
Xiaogang Cui, Tianqi Yuan, Zhengyu Fang, Jiao Feng, Changxin Wu
Summary: The study identified an miRNA, bta-miR-125a, that can control milk-fat production in cows by targeting the 3' untranslated region (UTR) of the SAA1 gene. The presence of miR-125a mimic decreased SAA1 expression and increased triglyceride and cholesterol content in bovine mammary epithelial cells.