Article
Immunology
Liang Zhao, Yibing Lv, Xiaoqi Zhou, Zilong Guo, Heli Li, Yanyan Guo, Tao Liu, Lei Tu, Liangru Zhu, Juan Tao, Guanxin Shen, Yong He, Ping Lei
Summary: The secreted form of 78-kDa glucose-regulated protein (sGRP78) has potential in the treatment of colitis. During the resolving phase, sGRP78 levels are reduced but quickly rebound, making it a potential candidate for the treatment of colitis. Administration of sGRP78 improves intestinal symptoms, restores TJ integrity, decreases immune cell infiltration, and impairs production of inflammatory cytokines.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Atieh Yaghoubi, Saeid Amel Jamehdar, Mohammad Reza Akbari Eidgahi, Kiarash Ghazvini
Summary: This study found that melittin peptide, alone or in combination with sulfasalazine, significantly cured clinical ulcerative colitis and attenuated inflammation and tissue damage in colon tissue. Furthermore, this peptide had an antioxidant effect by disrupting the oxidant/antioxidant balance.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Chemistry, Applied
Hye-Ryung Park, Dong Hyun Eom, Jae Hwan Kim, Jung-Cheul Shin, Myoung-Sook Shin, Kwang-Soon Shin
Summary: The study demonstrated the anti-inflammatory activity of NeoGOS-P70 in a DSS-induced colitis model, showing potential as a novel agent for ulcerative colitis treatment through improvements in symptoms, suppression of inflammatory mediators, and protective effects on colon tissue.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Catherine Verhaeghe, Marja Talikka, Alain Sewer, Nicolas Sierro, Mehdi Auberson, Dariusz Peric, David Bornand, Remi Dulize, Emmanuel Guedj, Patrick Nef, Sebastien P. Tabruyn, Julia Hoeng, Manuel C. Peitsch, Giuseppe Lo Sasso
Summary: This study investigated the potential anti-inflammatory activities of tobacco alkaloids nicotine and anatabine in a fully humanized UC mouse model. The results showed that nicotine significantly reduced acute colitis symptoms and improved colitis-specific endpoints. Anatabine also demonstrated similar effectiveness, although weaker or not significant. Gene expression analysis provided insights into the possible mechanism by which nicotine attenuated DSS-induced colitis in humanized mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Zecai Zhang, Yueqi Cui, Siyu Liu, Jiang Huang, Yu Liu, Yulong Zhou, Zhanbo Zhu
Summary: This study investigated the protective effects and potential mechanisms of zingerone (ZO) extracted from ginger on dextran sulfate sodium (DSS)-induced mouse ulcerative colitis (UC). The results showed that ZO alleviated weight loss, reduced disease activity index scores, and improved pathological changes in colon tissue. Mechanism analysis revealed that ZO inhibited the activation of the nuclear factor-kappa B pathway and regulated peroxisome proliferator-activated receptor gamma (PPAR gamma) expression. The findings suggest that ZO supplementation may be a new dietary strategy for UC treatment.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Food Science & Technology
Wenqiang Shen, Yaojun Liu, Xinyue Zhang, Xiong Zhang, Xiaoping Rong, Lihong Zhao, Cheng Ji, Yuanpei Lei, Fengjuan Li, Jing Chen, Qiugang Ma
Summary: In this study, the potential ameliorative effects of probiotic Bacillus subtilis and biodegradable Bacillus subtilis on ZEN toxicosis in gilts were compared. The results showed that the biodegradable Bacillus subtilis had significant protective effects on growth performance, immune function, hormone secretion, and ZEN elimination in gilts compared to the probiotic Bacillus subtilis.
Article
Biochemistry & Molecular Biology
Ho Won Kim, A-Reum Yu, Ji Won Lee, Hoe Sun Yoon, Byung Soo Lee, Hwan-Woo Park, Sung Ki Lee, Young Ik Lee, Jake Whang, Jong-Seok Kim
Summary: This study elucidates the role of the Acod1-itaconate axis in colitis for the first time. Deletion of Acod1 results in severe DSS-induced colitis and substantial increases in inflammatory cytokine and chemokine levels. The findings suggest that Acod1 may play an important regulatory role in the pathogenesis of colitis, demonstrating the potential for novel therapies using 4-OI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Emma Olivia Schultz Harringer, Juliana Durack, Yvette Piceno, Vibeke Andersen, Susan V. V. Lynch
Summary: Several studies have shown that a gluten-free diet can improve disease symptoms in ulcerative colitis (UC) patients. This study hypothesized that UC-associated enteric microbes metabolize dietary gluten differently, producing immunogenic products that promote inflammation. The study found that UC patients did not consume gluten differently from healthy controls, but the profile of gluten-degrading bacteria in their stool was distinct. The products of gluten degradation by certain bacteria induced inflammation and affected barrier function in colonocyte cultures. These preliminary findings suggest a need for further investigation into the mechanisms by which gut microbiota contribute to UC pathogenesis through gluten degradation.
Article
Multidisciplinary Sciences
Sarah Gingichashvili, Doron Steinberg, Ronit Vogt Sionov, Osnat Feuerstein, Noa E. Cohen
Summary: In this study, computational analytics were used to quantify the morphological response of Bacillus subtilis to the proximity of an antimicrobial source. The results revealed that the inner core, characterized by wrinkled formations, is more preserved compared to the periphery. Additionally, a crescent-shaped colony morphology and changes in the growth substrate of macrocolonies exposed to the antimicrobial were observed.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Mei-yan Wu, Wen-wei Li, Graham Christie, Peter Setlow, Yong-qing Li
Summary: Using Raman tweezers and live-cell phase-contrast microscopy, this study characterized the lysis process of individual Bacillus subtilis cells carrying PBSX prophage during spores' germination, outgrowth, and vegetative growth. The findings showed that nucleic acid intensities in induced cultures decreased rapidly after prophage induction, with a delay before cell burst, and the latent time and burst time varied in cell populations under different culture conditions.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Qilin Yu, Haohao Mao, Bowen Yang, Yahui Zhu, Cheng Sun, Zhiqiang Zhao, Yang Li, Yaobin Zhang
Summary: Electrical stimulation was found to enhance microbial extracellular electron transfer (EET), but the underlying reasons were unclear. This study showed that Bacillus subtilis, a gram-positive bacterium capable of extracellular respiration, had a higher EET capacity after electrical domestication. It was discovered that the polarization of amide groups induced by electrical stimulation promoted H-bond recombination and radical generation of protein-like substances, facilitating extracellular electron transfer via proton-coupled mechanism.
Article
Biochemical Research Methods
Kun Liu, Bingjie Jia, Liping Zhou, Lei Xing, Lvying Wu, Yuanyuan Li, Jungang Lu, Lei Zhang, Su Guan
Summary: This study utilized metabolomics and lipidomics to identify biomarkers for evaluating the therapeutic efficacy of mesalazine on ulcerative colitis, revealing potential mechanisms of treatment. Through serum metabolomics analysis, metabolites associated with treatment outcomes were discovered, alongside identification of dysregulated metabolic pathways in colitis.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Gastroenterology & Hepatology
Sarah C. Sasson, Stephanie M. Slevin, Vincent T. F. Cheung, Isar Nassiri, Anna Olsson-Brown, Eve Fryer, Ricardo C. Ferreira, Dominik Trzupek, Tarun Gupta, Lulia Al-Hillawi, Mari-lenna Issaias, Alistair Easton, Leticia Campo, Michael E. B. FitzPatrick, Joss Adams, Meenali Chitnis, Andrew Protheroe, Mark Tuthill, Nicholas Coupe, Alison Simmons, Miranda Payne, Mark R. Middleton, Simon P. L. Travis, Benjamin P. Fairfax, Paul Klenerman, Oliver Brain
Summary: Our study demonstrates that CD8(+) tissue resident memory T (T-RM) cells are the main activated T cell subset in ICI colitis. The pattern of gastrointestinal immunopathology in ICI colitis is distinct from ulcerative colitis at both the immune and epithelial-signaling levels. Activation of CD8+ T-RM cells correlates with clinical and endoscopic severity of ICI colitis.
Article
Chemistry, Multidisciplinary
Alice Miral, Sylvain Fournet, Catherine Porte, Aurelie Sauvager, Josselin Montarry, Sophie Tomasi, Sylvain Tranchimand
Summary: Healthy food is a major challenge for the development of this century, with plant-parasitic nematodes causing significant damage to global crop yields. The use of chemical nematicides for control is no longer environmentally sustainable, and biocontrol methods, such as biopesticides derived from living organisms, offer a promising alternative. This study focuses on the underexploited ecological niche of lichen microecosystems to develop new plant protection products. The lichen-associated bacterial suspensions from Paenibacillus etheri are investigated for their potential as nematicides against two nematode species, and the effects of volatile organic compounds produced by the bacteria are evaluated.
Article
Biochemistry & Molecular Biology
Zhiwei Tu, Henk L. Dekker, Winfried Roseboom, Bhagyashree N. Swarge, Peter Setlow, Stanley Brul, Gertjan Kramer
Summary: The research investigated the proteome dynamics during sporulation of Bacillus subtilis vegetative cells under nutrient limitation. Protein co-expression analysis identified four modules, with brown and green modules being upregulated during sporulation containing proteins associated with sporulation, while blue module showed negative correlation and contained ribosomal and metabolic proteins. Besides known transcription regulons, some proteins co-expressed with brown and green modules may also play roles during sporulation. Levels of some coat proteins decreased late in sporulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)