期刊
JOURNAL OF FUNCTIONAL FOODS
卷 20, 期 -, 页码 215-225出版社
ELSEVIER
DOI: 10.1016/j.jff.2015.10.027
关键词
Cytokines; Intestinal epithelium cells; Lactobacillus plantarum; Immunomodulatory effects; NF-kappa B; p38 MAPK
资金
- National Natural Science Foundation of China [31171718, 31100959, 31401513]
- National Science and Technology Project [2011AA100902]
- Science Foundation for Distinguished Young Scholars of Heilongjiang Province [JC201415]
- Promotion Program for Innovation of Science Research in Heilongjiang Province [YC13D005]
- National Key Technology Support Program [2013BAD18B11, 2012BAD29B07]
- Academic Backbone Project of Northeast Agricultural University [15XG26]
Lactobacillus has been used as a functional food ingredient to improve health. The expression of the pro-inflammatory cytokines interleukin-1 beta (IL-1 beta), IL-6 and tumour necrosis factor-alpha (TNF-alpha) stimulated by Lactobacillus plantarum NDC 75017 were investigated in the Caco-2 cells and intestinal tract of mice. In vitro, three cytokines (IL-1 beta, TNF-alpha, IL-6) could be momentarily induced; increased to the maximum level at 2, 4 and 8 h, respectively; and then decreased. Similar regulation could also be observed in vivo, and the expression of all cytokines peaked on day 5. Moreover, a transient activation of toll-like receptors (TLRs) and phosphorylation of NF-kappa B p65 and p38 MAPK were detected in Caco-2 cells by stimulation of NDC 75017. Inhibitors of NF-kappa B and p38 MAPK significantly suppressed cytokine expression (P < 0.01). Results suggested that L. plantarum NDC 75017 could perform a beneficial immunomodulation involving the pro-inflammatory cytokines NF-kappa B and p38 MAPK. This strain could be applied as a new potential immunoregulatory agent in functional foods. (C) 2015 Elsevier Ltd. All rights reserved.
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