Article
Food Science & Technology
Ying Liu, Yongpeng Jin, Qi Guo, Xiong Wang, Sunlin Luo, Wenjun Yang, Juntao Li, Yiqiang Chen
Summary: In this study, a LC-MS/MS method was developed for simultaneous determination of six mycotoxins in feed and feedstuff. The method showed good linearity, low detection limits, and was reliable for detecting mycotoxins in actual feed samples.
Article
Biochemical Research Methods
Shota Yoshida, Hongyan Zhang, Ryuji Takahashi, Sakura Yoshida, Yumi Abiko, Akira Toriba
Summary: This study identified non-polar compounds that coprecipitate with aflatoxins and developed an effective pretreatment method combining zirconia-coated silica gel and IAC. The method has been successfully applied for the quantification of aflatoxins in four types of samples and in real samples where mold growth was encouraged.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Wei-Jay Chen, Ren-Jang Wu, Hsueh-Hui Yang, Ahai-Chang Lua, Yin-Jeh Tzeng, Shun-Hsing Tuan, Liang Chen, Tsong-Yung Chou
Summary: The abuse of PMMA and MDMA among young people is a growing public health problem. A simple method for simultaneous enantiomeric determination of PMMA and MDMA has been developed, which is rapid, environmentally friendly, and safe.
Article
Chemistry, Applied
Kai Yao, Jing Zhang, Yumin Niu, Xin Zhang, Yunjia Yang, Yige Wu, Kai Wen, Bing Shao
Summary: The European Food Safety Authority (EFSA) has proposed a temporary tolerable daily intake of 0.04 ng/kg body weight/day for Bisphenol A (BPA) and its four analogues due to their potential endocrine disrupting effects. A more sensitive analytical method was urgently needed for the risk reassessment of bisphenols (BPs). The matrix effect of Chinese foods, which contain various spices, is a challenge for the analysis of ultra-trace analytes. In this study, a multi-immunoaffinity column (mIAC) combined with ultra-high-performance liquid chromatography tandem mass spectrometry detection (UHLPC-MS/MS) was used for the purification and analysis of BPA, BPB, BPF, BPS, and BPAF in Chinese foods.
Article
Toxicology
Katerina Vlasakova, Andreas Steinhilber, Wendy J. Bailey, Zoltan Erdos, Hanna Haag, Thomas Joos, Bradley L. Ackermann, Oliver Poetz, Warren E. Glaab
Summary: This study reports a novel liquid chromatography mass spectrometric assay for biomarkers of drug-induced pancreatic injury. The assay, based on immunoprecipitation of peptides and mass spectrometry analysis, showed higher sensitivity and specificity compared to traditional enzyme detection methods.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Chemistry, Applied
Yared Getachew Lijalem, Mohamed A. Gab-Allah, Kihwan Choi, Byungjoo Kim
Summary: A method using isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC/MS/MS) was developed for the accurate determination of zearalenone (ZEN) and its metabolites in corn. The method demonstrated sufficient selectivity, sensitivity, accuracy, and precision.
Article
Chemistry, Analytical
Ainan Zhou, Jinghua Yu, Yali Wu, Hao Xue, Dafang Zhong, Xingxing Diao
Summary: We aimed to establish an LC-MS/MS method for quantifying bevacizumab content in biological fluids using a surrogate peptide generated through trypsin hydrolysis and an isotopically labeled peptide as an internal standard. The method involved a series of pre-treatment processes and had a total duration of approximately 2.5-3 h. The validated method showed a linear range of 0.2 μg/mL to 300 μg/mL in different matrices, with satisfactory precision and accuracy.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Xiaojuan Li, Li Xiao, Brent Kochert, Daniel P. Donnelly, Xinliu Gao, Douglas Richardson
Summary: The study explored unpaired cysteines in a therapeutic antibody mAb1 using LC-MS methods, revealing different variants containing unpaired cysteines in domain and peptide mapping analysis, unaffected by high pH and heat-stressed conditions. The integrated LC-MS methods, stress studies, and structural analysis could potentially be applied to analyze unpaired cysteines in other mAbs.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biochemical Research Methods
Mingjie Yu, Zaiming Liang, Lirong Xiong, Fang Liu, Lin Cheng, Changsheng Jia, Xiaowei Qi, Fengjun Sun
Summary: This study developed and validated a highly sensitive, rapid, and stable method for quantifying the concentration of Anlotinib in human plasma. Therapeutic drug monitoring can be used to determine the peak concentration of Anlotinib in the plasma of patients with different types of tumors.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Food Science & Technology
B. Choi, J. H. Kim, K. S. Lee, C. Kim, J. Y. Lee, H. M. Park
Summary: This study aimed to validate the analytical method for detecting multiple mycotoxins in roasted coffee samples. The method used liquid chromatography-tandem mass spectrometry and an immunoaffinity column for sample pre-treatment. The results showed that the validated method was suitable for multimycotoxin detection in roasted coffee samples.
INTERNATIONAL FOOD RESEARCH JOURNAL
(2023)
Article
Chemistry, Applied
Enhui Guo, Linchuan Zhao, Kang Wu, Wen Huang, Kang Zhao, Jianguo Li, Anping Deng
Summary: The study optimized the conditions for the simultaneous extraction of chloramphenicol, florfenicol and thiamphenicol using immunoaffinity clean-up with two specific monoclonal antibodies. LC-MS/MS coupled with immunoaffinity clean-up showed higher extraction efficiency and sensitivity compared to solid phase extraction (SPE) for the detection of the three antibiotics.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2021)
Article
Food Science & Technology
Gustavo A. Figueroa Campos, Johannes G. K. T. Kruizenga, Sorel Tchewonpi Sagu, Steffen Schwarz, Thomas Homann, Andreas Taubert, Harshadrai M. Rawel
Summary: This study investigated the changes in the protein fraction of coffee, which is important for cup quality. The results showed a significant increase in modified peptides from wet processed green beans compared to dry ones. This study contributes to a better understanding of the influence of different processing methods on protein quality and its role in coffee cup quality and aroma.
Article
Medicine, Research & Experimental
Cheng Hu, Tao Wang, Xiaoyu Zhuang, Qiaoli Sun, Xiaochun Wang, Hui Lin, Mingli Feng, Jiaqi Zhang, Qin Cao, Yuanye Jiang
Summary: Through high-throughput mass spectrometry analysis, this study identified 15 serum metabolites strongly associated with early-stage NAFLD, which may have significant clinical implications for early diagnosis and monitoring response to therapeutic interventions for NAFLD.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ghaith M. Hamza, Eric Miele, Don M. Wojchowski, Paul Toran, Camilla R. Worsfold, Tamil S. Anthonymuthu, Vladislav B. Bergo, Andrew X. Zhang, Jeffrey C. Silva
Summary: The Affi-BAMS™ epitope-directed affinity bead capture/MALDI MS platform is effective in quantitatively defining complex PTM marks of H3 and H4 histones. The platform achieves a range of >3 orders of magnitude with a technical precision CV of <5%. It can resolve heterogeneous histone N-terminal PTMs with as little as 100 μg of starting material.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Liangjun Hu, Na Liu, Yanchao Li, Qiancheng Zhang, Xiaobing Cui, Chenxiao Shan, Shengjun Chen, Song Li, Rui Liu, Hongmei Wen
Summary: This study developed a novel and effective strategy for analyzing and authenticating Guang Dilong, a traditional Chinese medicine, based on enzymatic digestion of protein. Peptidomics profiles of trypsin-digested samples were evaluated using nanoLC-MS/MS, resulting in the identification of species-specific peptide biomarkers in Guang Dilong. Mathematical set theory was used to investigate the significance of different samples and peptides in the target species set. Finally, five specific peptide biomarkers were confirmed and validated for differentiating Guang Dilong from other species using UFLC-MS/MS and MRM mode.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
