Article
Biochemical Research Methods
Yuhuan Ji, Zhaoxu Guo, Min Yan, Limin Chu, Min Meng, Yantao Chu, Hong Yu, Laixin Wang
Summary: Two LC-based bioanalytical methods were developed and validated to evaluate the ADME and PK/PD properties of RBD1016 siRNA. The results showed that both methods had excellent accuracy, precision, specificity, and reproducibility.
Review
Biochemistry & Molecular Biology
Aowen Liu, Ming Cheng, Yixuan Zhou, Pan Deng
Summary: Oligonucleotides (OGNs) are a relatively new modality that provides unique opportunities for expanding therapeutic targets. Liquid chromatography-mass spectrometry (LC-MS) is now becoming the preferred method for bioanalysis of OGNs. While ion pairing reversed-phase liquid chromatography (IP-RPLC) has been widely used, there are technical issues associated with these methods. IP-free methods, such as hydrophilic interaction liquid chromatography (HILIC) and anion-exchange techniques, have emerged as promising approaches. In this review, the state-of-the-art IP-RPLC-MS bioanalytical methods for OGNs and their metabolites published in the past 10 years (2012-2022) are critically reviewed. Recent advances in IP-reagent-free LC-MS bioanalysis methods are discussed. Finally, future opportunities for developing new methods for comprehensive bioanalysis of OGNs are described.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Feiyang Li, Shenkai Chen, Sylwia Studzinska, Michael Laemmerhofer
Summary: Ion-pairing reversed-phase liquid chromatography (IP-RPLC) is commonly used for the analysis of nucleic acid related compounds. However, the high concentration of ion-pairing reagent in IP-RPLC is not suitable for coupling with mass spectrometry (MS). This study explored the use of a PBT-bonded silica-based stationary phase for the separation of Patisiran without the need for ion-pairing reagents. The method allowed for MS and MS/MS characterization without contamination or related issues.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Sophia Barhdadi, Patricia Courselle, Eric Deconinck, Celine Vanhee
Summary: The use of CBD products has significantly increased in the past five years due to their reputed therapeutic benefits and lack of psychoactive effects. A study in Belgium found that CBD e-liquids with a THC content below 0.2% are legal, but only 30% of the investigated samples contained the labeled amount of CBD within a 10% deviation. Furthermore, CBD e-liquids labeled full/broad spectrum had minor alkaloids present compared to classic CBD e-liquids. These results highlight the need for quality control measures in CBD e-liquids.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Long Yuan, Jean-Francois Dupuis, Kevork Mekhssian
Summary: The study developed a hybridization LC-MS/MS method for the quantification of double-stranded oligonucleotides. The results showed that the peptide nucleic acid (PNA) probe achieved better extraction recovery than the commonly used DNA capture probe. The optimized hybridization method using the PNA probe was successfully applied for the quantitation of siRNA-01 in biological samples.
Article
Agriculture, Multidisciplinary
Maria Teresa Senatore, Antonio Prodi, Francesco Tini, Virgilio Balmas, Alessandro Infantino, Andrea Onofri, Eleonora Cappelletti, Safa Oufensou, Michael Sulyok, Lorenzo Covarelli, Giovanni Beccari
Summary: This study assessed the evolution of fungal communities associated with durum wheat using different diagnostic approaches. The results showed that qPCR was the best method for predicting mycotoxin contamination in grains for some Fusarium species. The presence of Alternaria and Fusarium mycotoxins, particularly enniatins, was found in the grain harvested in central Italy, while deoxynivalenol was mainly detected in northern-central Italy.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemical Research Methods
Pei Li, Jean-Francois Dupuis, Valerie Vrionis, Kevork Mekhssian, Tom Magee, Long Yuan
Summary: The GLP validation and application of ASO bioanalysis using the hybridization LC-MS/MS platform was reported for the first time. The method, combining the advantages of ligand binding and LC-MS/MS, successfully quantified an ASO drug candidate in monkey serum, CSF, and tissues.
Review
Spectroscopy
Soheil Pourshahian
Summary: This article discusses the synthesis, impurities, and degradation products of oligonucleotides, as well as the application of liquid chromatography combined with mass spectrometry in the characterization and quantification of these substances. It also examines methods for determining oligonucleotide sequences through gas-phase fragmentation and indirect mass spectrometric methods.
MASS SPECTROMETRY REVIEWS
(2021)
Article
Engineering, Environmental
Sanjeeb Mohapatra, Neha Sharma, Gayatree Mohapatra, Lokesh P. Padhye, Suparna Mukherji
Summary: In this study, wastewater samples from five WWTPs in western India were analyzed using fluorescence spectroscopy. The hydrophilic acid and hydrophilic neutral fractions were found to be more abundant than the hydrophilic base fraction in both influent and effluent wastewater streams. Trphtophan-like and tyrosine-like substances were also detected in the wastewater. LC-MS/MS analysis identified hundreds of DOM proteins in the influent and effluent streams, some of which were related to Alzheimer's and Huntington disease, serving as potential biomarkers for wastewater-based epidemiology.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Engineering, Environmental
Mark A. Borchardt, Alexandria B. Boehm, Marc Salit, Susan K. Spencer, Krista R. Wigginton, Rachel T. Noble
Summary: Real-time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) methods have revolutionized environmental microbiology by providing quantitative organism-specific data. However, many researchers in the field fail to report necessary controls and methods, impacting the reliability of their study results. By implementing systematic controls, efficient workflow, and critical reporting elements, data credibility in environmental microbiology research can be enhanced to support disease prevention efforts.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Plant Sciences
Pierluigi Reveglia, Regina Billones-Baaijens, Jennifer Millera Niem, Marco Masi, Alessio Cimmino, Antonio Evidente, Sandra Savocchia
Summary: This study investigated the role of phytotoxic metabolites (PMs) in the expression of Botryosphaeria dieback (BD) symptoms in grapevines. It was found that (R)-mellein may play a key role in the disease process, while protocatechuic acid and spencertoxin were not detected in the inoculated wood samples.
Article
Pharmacology & Pharmacy
Hiroki Tanaka, Nae Takata, Yu Sakurai, Tokuyuki Yoshida, Takao Inoue, Shinya Tamagawa, Yuta Nakai, Kota Tange, Hiroki Yoshioka, Masatoshi Maeki, Manabu Tokeshi, Hidetaka Akita
Summary: This study investigated the optimal lipid composition of LNPs for the delivery of siRNA and ASO, finding that the optimal compositions for siRNA and ASO were different and differed from those reported for mRNA in previous studies. The key processes for successful delivery of mRNA, siRNA, and ASO were identified as extracellular stability, endosomal escape, and cellular uptake, respectively, indicating the importance of optimizing LNP lipid compositions based on the type of nucleic acids being delivered.
Article
Food Science & Technology
Jinrui Zhu, Li Zhou, Minjie Zhao, Fang Wei, Haiyan Fu, Eric Marchioni
Summary: This study investigated the dynamic changes in lipid composition during the roasting process of coffee beans using lipidomic analysis. Multiple lipid classes and molecular species were identified and quantified, showing distinct changes during roasting.
FOOD RESEARCH INTERNATIONAL
(2023)
Review
Medicine, General & Internal
Julien Dugal-Tessier, Srinath Thirumalairajan, Nareshkumar Jain
Summary: This article summarizes the key technological advancements in the preparation of antibody-oligonucleotide conjugates (AOCs) and discusses the distinct advantages and disadvantages of AOCs as novel therapeutics. It evaluates the merits and demerits of different methods of conjugating oligonucleotides to antibodies, mainly focusing on AOC purification and analytical characterizations. Lessons learned from in vitro and in vivo studies, particularly findings related to silencing, trafficking, and cytotoxicity of the conjugates, are also summarized.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Carme Fabrega, Anna Avino, Natalia Navarro, Andreia F. Jorge, Santiago Grijalvo, Ramon Eritja
Summary: In this study, the conjugation of oligonucleotides with lipids and peptides for the development of therapeutics with improved pharmacokinetics is reviewed.
