Article
Chemistry, Medicinal
Alexander O. Pritchard, Dion B. Nemez, David E. Herbert, Shyamala Dakshinamurti, John L. Sorensen
Summary: This article presents a straightforward purification method of forskolin from commercially available weight loss capsules, addressing ambiguities in the use of the name 'forskolin'. The complete spectroscopic characterization and single crystal X-ray structure of purified forskolin is detailed in this report.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemical Research Methods
Pushpendra Kumar Shukla, Ankita Misra, Akanksha Srivastava, Manish Kumar, Sharad Srivastava
Summary: Coleus forskohlii Briq. is an important medicinal herb with a wide range of medicinal properties. The bioactive compound content varies due to soil nature and environmental factors, and different germplasms have different levels of forskolin and iso-forskolin. The plant exhibits high antioxidant and antidiabetic activities. The aim of this study is to enhance the production of bioactive compounds and identify the best germplasm for commercial cultivation using elicitors and precursors.
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2022)
Article
Biology
Vikram Bhatia, Saeid Maghsoudi, Martha Hinton, Anjali Y. Y. Bhagirath, Nisha Singh, Appalaraju Jaggupilli, Prashen Chelikani, Shyamala Dakshinamurti
Summary: This study investigated the structure of adenylyl cyclase 6 and its interactions with forskolin. Mutations were made to identify the amino acids responsible for activating the enzyme. The findings could contribute to the design of selective activators for adenylyl cyclase 6.
Article
Cell Biology
Gabriel Komla Adzika, Hongjian Hou, Adebayo Oluwafemi Adekunle, Ruqayya Rizvi, Seyram Yao Adzraku, Kexue Li, Qi-Ming Deng, Richard Mprah, Marie Louise Ndzie Noah, Joseph Adu-Amankwaah, Jeremiah Ong'achwa Machuki, Wenkang Shang, Tongtong Ma, Stephane Koda, Xianluo Ma, Hong Sun
Summary: The study aimed to prevent pathological cardiac hypertrophy during chronic stress by modulating cardiac and inflammatory responses. The combination of Amlexanox and Forskolin showed the highest efficacy in regulating adaptive inflammatory responses.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Katherine M. Gerber, Nicholas B. Whitticar, Daniel R. Rochester, Kathryn L. Corbin, William J. Koch, Craig S. Nunemaker
Summary: The study investigates the ability of islets to secrete insulin above the traditionally accepted glucose concentration levels. Results show that in extreme hyperglycemia, islets can enhance insulin secretion through amplifying pathways, and glucose concentration can impact the level of insulin secretion.
Article
Biochemistry & Molecular Biology
Thi Mong Diep Nguyen, Laura Filliatreau, Daniele Klett, Nong Van Hai, Nguyen Thuy Duong, Yves Combarnous
Summary: The research reveals that ADCY10 is located in the cytoplasm and responds to four different inhibitors. These inhibitors can reduce cAMP accumulation in MTLC-1 cells and various Leydig cells, emphasizing the importance of ADCY10 in response to the hormone LH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Xuefeng Zhang, Alejandro Pizzoni, Kyoungja Hong, Nyla Naim, Chao Qi, Volodymyr Korkhov, Daniel L. Altschuler
Summary: CAP1 is a highly conserved protein involved in regulating cAMP dynamics in mammalian cells by binding and activating adenylyl cyclase, modulating cAMP levels in a Rap1-dependent manner, and affecting cAMP-dependent proliferation. The interaction between CAP1, cyclase, and Rap1 forms a regulatory unit that may be involved in establishing cAMP microdomains and localized signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Kirstin L. Brown, Kaley M. Wilburn, Christine R. Montague, Jason C. Grigg, Olalla Sanz, Esther Perez-Herran, David Barros, Lluis Ballell, Brian C. VanderVen, Lindsay D. Eltis
Summary: GSK286 acts as an agonist of Rv1625c, a membrane-bound adenylyl cyclase, to inhibit the growth of Mycobacterium tuberculosis and increase intracellular cAMP levels. However, the detailed mechanism of action of these compounds still needs to be elucidated.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Pharmacology & Pharmacy
Gianna Giacoletti, Tatum Price, Lucas V. B. Hoelz, Abdulwhab Shremo Msdi, Samantha Cossin, Katerina Vazquez-Falto, Tacio V. Amorim Fernandes, Vinicius Santos de Pontes, Hongbing Wang, Nubia Boechat, Adwoa Nornoo, Tarsis F. Brust
Summary: In this study, the AC1-selective inhibitor ST034307 was found to alleviate pain in mouse models without causing analgesic tolerance. While unable to detect ST034307 in the mouse brain, a significant reduction in cAMP concentration was observed in the dorsal root ganglia of the animals. These results indicate that AC1 inhibitors are a promising class of analgesic agents that effectively treat pain without inducing tolerance.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biology
Hanako Hagio, Wataru Koyama, Shiori Hosaka, Aysenur Deniz Song, Janchiv Narantsatsral, Koji Matsuda, Takashi Shimizu, Shoko Hososhima, Satoshi P. Tsunoda, Hideki Kandori, Masahiko Hibi
Summary: In this study, a combination of microbial proteins including GtCCR4, KnChR, BeGC1, and PACs were used to control cell functions in zebrafish. Activation of GtCCR4 and KnChR induced swimming behavior in a short time, while activation of BeGC1 or PACs had a longer latency. Additionally, activation of GtCCR4 and KnChR caused cardiac arrest, while activation of bPAC gradually slowed down the heart rate.
Article
Pharmacology & Pharmacy
Kayla Johnson, Alexis Doucette, Alexis Edwards, Aleeya Verdi, Ryan McFarland, Shelby Hulke, Amanda Fowler, Val J. Watts, Amanda H. Klein
Summary: This study found that reducing the activity or expression of adenylyl cyclase 1 can attenuate morphine tolerance and hypersensitivity, as well as provide relief for inflammatory pain.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Yuening Liu, Jingrui Chen, Shayne K. Fontes, Erika N. Bautista, Zhaokang Cheng
Summary: Protein kinase A (PKA) serves as a central regulator of cardiac performance and morphology. Abnormal activation or inactivation of PKA is associated with a wide range of heart diseases, and PKA could be a promising drug target for cardioprotection. Therefore, specific PKA inhibitors or activators may represent valuable drug candidates for the treatment of heart diseases.
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Milica Gusic, Klaus Benndorf, Christian Sattler
Summary: Our study reveals that each occupied binding site contributes to channel activation in P2X7 receptors. While the occupation of a single binding site can activate the channels, three bound ligands maximally stabilize the open state, suggesting a stepwise activation process for P2X7 receptors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Engineering, Biomedical
Audrey Franceschi Biagioni, Giada Cellot, Elisa Pati, Neus Lozano, Belen Ballesteros, Raffaele Casani, Norberto Cysne Coimbra, Kostas Kostarelos, Laura Ballerini
Summary: Engineered small graphene oxide (s-GO) sheets have been shown to reversibly down-regulate glutamatergic synapses in the hippocampus, indicating their potential to target specific synapses in vivo. Synapses, acting as functional interfaces among neurons in the Central Nervous System (CNS), play a crucial role in synaptic plasticity, which is essential for learning and memory. Dysfunctional synaptic plasticity has been implicated in various brain diseases, such as dementia and anxiety disorders, highlighting the importance of understanding these mechanisms for potential therapeutic interventions.
Article
Instruments & Instrumentation
Ian Delgadillo Bonequi, Abraham Stroschein, Lucas J. Koerner
Summary: This article describes a custom and open-source FPGA-based data acquisition system that is used for electrophysiology and closed-loop feedback experiments. The system combines FPGA acquisition and processing with high-speed analog and digital converters to enable real-time feedback. The digital approach simplifies experimental setup and repeatability by allowing for system identification and in situ tuning of filter bandwidths.
REVIEW OF SCIENTIFIC INSTRUMENTS
(2022)
