Article
Biochemistry & Molecular Biology
Ryan Lane, Anouk H. G. Wolters, Ben N. G. Giepmans, Jacob P. Hoogenboom
Summary: We developed a 3D image acquisition and reconstruction pipeline that integrates widefield fluorescence microscopy with scanning electron microscopy, overcoming the limitations of slow acquisition rates and difficulty in providing targeted biological information. By using cathodoluminescent markers, we achieved high precision EM-FM overlay. Our proof-of-concept on immunolabelled serial sections of tissues demonstrated expedited acquisition times and reduced data burden.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hong-Lim Kim, Tae-Ryong Riew, Jieun Park, Youngchun Lee, In-Beom Kim
Summary: Researchers have developed a new method by combining light and electron microscopy with immunolabeling, to overcome technical barriers in immuno-electron microscopy. This method allows for excellent antigenicity of target proteins and preservation of ultrastructure, without the need for specifically designed correlative light and electron microscopy devices. It can be easily applied in many biomedical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Anatomy & Morphology
Hugh Tanner, Olivia Sherwin, Paul Verkade
Summary: Imaging is a crucial technology in biomedical research, but each technique only offers specific information. The combination of light and electron microscopy in CLEM allows for the advantages of both techniques. However, finding suitable markers for a Correlative Microscopy workflow remains a challenge.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Multidisciplinary Sciences
Jing Wang, Steven Randolph, Qian Wu, Aurelien Botman, Jenna Schardt, Cedric Bouchet-Marquis, Xiaolin Nan, Chad Rue, Marcus Straw
Summary: A CLEM workflow based on a novel FIB/SEM technology using an OSSM process was proposed, which achieved high resolution and imaging efficiency while improving throughput. By combining with iPALM technique, successful 3D imaging of mitochondria was carried out, demonstrating the advantages of OSSM-SEM in CLEM applications.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Sigurdur Runar Gudmundsson, Katri A. Kallio, Helena Vihinen, Eija Jokitalo, Nicholas Ktistakis, Eeva-Liisa Eskelinen
Summary: This study used live-cell imaging and electron microscopy techniques to provide high-resolution morphology of phagophore precursors in three dimensions. It revealed that phagophores are already closed or nearly closed into autophagosomes at the stage when the omegasome diameter is still large. The phagophore precursors emerged as bud-like highly curved membrane cisterns next to the endoplasmic reticulum with a small opening to the cytosol, and then opened to form more flat cisterns that elongate and curve to form crescent-shaped phagophores.
Article
Biochemistry & Molecular Biology
Davis Perez, Peter D. Dahlberg, Jiarui Wang, Annina M. Sartor, Julia S. Borden, Lucy Shapiro, W. E. Moerner
Summary: Cryo-CLEM combines cryo-EM and fluorescence microscopy to study cell structures and cellular environments simultaneously. In this study, the researchers used a fluorescent biosensor called roGFP2 to estimate the redox potential at the time of freezing by measuring the ratio of its emission brightness. By generating a calibration curve, they were able to investigate the oxidation/reduction state of polar organizing protein Z (PopZ) in vitrified Caulobacter crescentus cells.
JOURNAL OF STRUCTURAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Francesca Pagliari, Elisa Sogne, Davide Panella, Gerardo Perozziello, Carlo Liberale, Gobind Das, Alice Turdo, Simone Di Franco, Joao Seco, Andrea Falqui, Santo Gratteri, Arturo Pujia, Enzo Di Fabrizio, Patrizio Candeloro, Luca Tirinato
Summary: Melanoma is a type of tumor with a high percentage of cancer stem cells. Researchers have identified lipid droplets as one of the markers for these cells. They have used various techniques, such as mass spectrometry and imaging techniques, to characterize the droplets and developed a new experimental approach to study them.
Review
Microscopy
Christopher J. Guerin, Saskia Lippens
Summary: Correlative light and electron microscopy is a valuable tool that connects data on structure and function, allowing imaging across different resolution scales. Recent developments in this area have enabled its application to large volumes of cells and tissues, largely due to collaborative efforts between microscopists and commercial companies. This example demonstrates how niche technologies can be brought into the mainstream through the pioneering efforts of imaging specialists.
JOURNAL OF MICROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Jie E. Yang, Matthew R. Larson, Bryan S. Sibert, Samantha Shrum, Elizabeth R. Wright
Summary: Cryo-correlative light and electron microscopy (CLEM) combines the advantages of fluorescence light microscopy (FLM) and cryo-electron microscopy (cryo-EM) for high-resolution ultrastructure analysis of biological samples. Challenges lie in implementing correlation workflows and software tools on different microscope platforms to support automated cryo-EM data acquisition. CorRelator is an open-source desktop application that bridges between cryo-FLM and real-time cryo-EM/ET automated data collection, providing flexible and high accuracy correlation for cryo-CLEM workflows.
JOURNAL OF STRUCTURAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Simon Peters, Lena Kaiser, Julian Fink, Fabian Schumacher, Veronika Perschin, Jan Schlegel, Markus Sauer, Christian Stigloher, Burkhard Kleuser, Juergen Seibel, Alexandra Schubert-Unkmeir
Summary: Sphingolipids, including ceramides, have antimicrobial activity against a broad range of pathogenic microorganisms, with their mechanism of action being unclear. Using click-AT-CLEM imaging and mass spectrometry, it was found that azido-tagged sphingolipids efficiently incorporate into the outer membrane of Gram-negative bacteria, which is the underlying cause of their antimicrobial activity.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Kem A. Sochacki, Justin W. Taraska
Summary: Researchers use emerging CLEM techniques to study the assembly of protein coats inside cells. Current and developing CLEM technologies are poised to answer fundamental questions about protein coat architecture at the nanoscale.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gabriele Bongiovanni, Oliver F. Harder, Marcel Drabbels, Ulrich J. Lorenz
Summary: This study introduces a novel approach to time-resolved cryo-electron microscopy with microsecond time resolution. It involves melting a cryo sample with a laser beam to allow dynamics of the embedded particles. The sample revitrifies within microseconds after the laser beam is switched off, trapping the particles in their transient configurations, which can be imaged to obtain a snap shot of the dynamics at that point in time.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Physical
Johannes Zeininger, Philipp Winkler, Maximilian Raab, Yuri Suchorski, Mauricio J. Prieto, Liviu C. Tanase, Lucas de Souza Caldas, Aarti Tiwari, Thomas Schmidt, Michael Stoger-Pollach, Andreas Steiger-Thirsfeld, Beatriz Roldan Cuenya, Guenther Rupprechter
Summary: In this study, spatial-temporal nonuniformities in H-2 oxidation on individual Rh(hkl) domains of a polycrystalline Rh foil were investigated using PEEM, XPEEM, and LEEM. The latter two techniques were used for in situ correlative microscopy, which provided higher lateral resolution and enabled the detection of island-mediated oxygen front propagation during kinetic transitions. The origin of this phenomenon was rationalized through model calculations based on a hybrid approach of microkinetic modeling and Monte Carlo simulations.
Article
Multidisciplinary Sciences
Zhaohong Mi, Ce-Belle Chen, Hong Qi Tan, Yanxin Dou, Chengyuan Yang, Shuvan Prashant Turaga, Minqin Ren, Saumitra K. Vajandar, Gin Hao Yuen, Thomas Osipowicz, Frank Watt, Andrew A. Bettiol
Summary: Researchers efficiently excited nanodiamonds using a focused beam of helium ions, resulting in ionoluminescence. This was used for quantification and correlative localization of single particles within a whole cell at sub-30 nm resolution, and for investigating nanodiamond radiosensitisation effects.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Jie E. Yang, Matthew R. Larson, Bryan S. Sibert, Joseph Y. Kim, Daniel Parrell, Juan C. Sanchez, Victoria Pappas, Anil Kumar, Kai Cai, Keith Thompson, Elizabeth R. Wright
Summary: In this paper, robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens are presented, which can image large fields of view while preserving high-resolution structural information. The combination of multiple techniques in MPACT supports studies that contextually define the three-dimensional architecture of cells, and it provides flexibility and efficient data processing and analysis.
Editorial Material
Chemistry, Multidisciplinary
Daphne M. van Elsland, Sander I. van Kasteren
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Chemistry, Multidisciplinary
Frank Versluis, Daphne M. van Elsland, Serhii Mytnyk, Dayinta L. Perrier, Fanny Trausel, Jos M. Poolman, Chandan Maity, Vincent A. A. le Sage, Sander I. van Kasteren, Jan H. van Esch, Rienk Eelkema
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2016)
Article
Microscopy
D. M. Van Elsland, E. Bos, J. B. Pawlak, H. S. Overkleeft, A. J. Koster, S. I. Van Kasteren
JOURNAL OF MICROSCOPY
(2017)
Article
Biochemistry & Molecular Biology
Thomas Bakkum, Tyrza van Leeuwen, Alexi J. C. Sarris, Daphne M. van Elsland, Dimitrios Poulcharidis, Herman S. Overkleeft, Sander I. van Kasteren
ACS CHEMICAL BIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Daphne van Elsland, Jacques Neefjes
Article
Biochemistry & Molecular Biology
Marlieke L. M. Jongsma, Jeroen Bakker, Birol Cabukusta, Nalan Liv, Daphne van Elsland, Job Fermie, Jimmy L. L. Akkermans, Coenraad Kuijl, Sabina Y. van der Zanden, Lennert Janssen, Denise Hoogzaad, Rik van der Kant, Ruud H. Wijdeven, Judith Klumperman, Ilana Berlin, Jacques Neefjes
Article
Biochemistry & Molecular Biology
Priscillia Perrin, Lennert Janssen, Hans Janssen, Bram van den Broek, Lennard M. Voortman, Daphne van Elsland, Ilana Berlin, Jacques Neefjes
Summary: The endosomal system is a dynamic vesicle network for material and signal exchange between the cell and its environment. The cargoes in endosomes can be either degraded or secreted, with the possibility of retrofusion of intraluminal membranes back to the limiting membrane. This retrofusion process supports membrane equilibrium within the multivesicular body (MVB).
Article
Multidisciplinary Sciences
Dick J. H. van den Boomen, Agata Sienkiewicz, Ilana Berlin, Marlieke L. M. Jongsma, Daphne M. van Elsland, J. Paul Luzio, Jacques J. C. Neefjes, Paul J. Lehner
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Alexander M. Prophet, Kritanjan Polley, Gary J. Van Berkel, David T. Limmer, Kevin R. Wilson
Summary: The oxidation kinetics of iodide by ozone at the air-water interface is studied in single microdroplets. Molecular simulations and kinetic modeling are used to understand the underlying multiphase mechanism.
Article
Chemistry, Multidisciplinary
Rong Zhang, Jiajing Lan, Fei Wang, Shumei Chen, Jian Zhang
Summary: By utilizing 1,1'-ferrocene dicarboxylic acid as a chelating and surface protection ligand, we have synthesized multi-nuclear indium oxide clusters with varying nuclear sizes, including heptanuclear and thirteen-nuclear clusters. These clusters possess labile coordination sites, allowing for structural modification and self-assembly, resulting in the formation of various cluster structures.
Article
Chemistry, Multidisciplinary
Hui Wang, Hui Liu, Mingsen Wang, Jiaheng Hou, Yongjun Li, Yuancheng Wang, Yingjie Zhao
Summary: Two supramolecular complexes were prepared using CBs and M1, and their single-crystal structures were analyzed by SCXRD. The unexpected 1:2 self-assembly structure between M1 and CB[8] was discovered for the first time. These complexes exhibit unique photophysical properties and provide valuable information about the structure and photophysical properties of supramolecular complexes.
Article
Chemistry, Multidisciplinary
Chang-Hee Lee, Sookil Park, Sanggil Kim, Ji Young Hyun, Hyun Soo Lee, Injae Shin
Summary: The epidermal growth factor receptor (EGFR) is a cell-surface glycoprotein involved in cell proliferation and tumor development. This study used a fluorescently labeled EGFR to investigate its time-dependent endocytosis in live cells and found that appended glycans affect EGFR internalization. Additionally, the study detected sialic acid residues attached to EGFR on the live cell surface using FRET-based imaging. This research provides valuable insights into the cellular functions of EGFR.
Article
Chemistry, Multidisciplinary
Subhankar Sahu, Lokesh Kumar, Sumita Das, Dipti Gupta, Ruchi Anand
Summary: This study proposes a strategy that combines organic electronics with biosensor scaffolds to create a compact device for monitoring environmental aromatic pollution. By coupling biosensing protein MopR with an organic electrochemical transistor (OECT), a sensor module capable of efficient detection of phenol was designed. Exclusive phenol detection with minimal loss of sensitivity could be achieved in complex pollutant mixtures and real environmental samples.
Review
Chemistry, Multidisciplinary
Changseok Lee, Hyung-Joon Kang, Sungwoo Hong
Summary: The formation of C-N bonds through hydroamination reactions catalyzed by nickel hydrides has been a topic of recent interest. This approach offers a way to efficiently transform a variety of alkene and alkyne substrates into compounds enriched with C-N bonds. The review provides a concise overview of the underlying reaction mechanisms and aims to stimulate further progress in NiH-catalytic techniques and catalyst design.
Article
Chemistry, Multidisciplinary
Yueci Wu, Lu-Lu Sun, Hai-Hao Han, Xiao-Peng He, Weiguo Cao, Tony D. James
Summary: Drug-induced liver injury (DILI) is a common cause of acute liver failure in the USA and Europe, but most cases can be recovered or prevented by discontinuing the offending drug. Recent research has found that peroxynitrite (ONOO-) can be used as a potential indicator for early diagnosis of DILI, and there is an urgent need to establish a method to detect and track peroxynitrite in DILI cases. In this study, a FRET-based nano fluorescent probe CD-N-I was developed, which showed high selectivity and sensitivity in detecting peroxynitrite. The probe successfully detected exogenous peroxynitrite in live cells and endogenous peroxynitrite in APAP-induced liver injury of HepG2 cells.
Article
Chemistry, Multidisciplinary
Dmitry L. Lipilin, Mikhail O. Zubkov, Mikhail D. Kosobokov, Alexander D. Dilman
Summary: This article describes a direct photocatalytic method for the thiolation of unprotected acids, which was previously challenging. By using a thionocarbonate reagent with an N-O bond, the efficient conversion of carboxylic acids to thiols is achieved.
Article
Chemistry, Multidisciplinary
Jason Malenfant, Lucille Kuster, Yohann Gagne, Kouassi Signo, Maxime Denis, Sylvain Canesi, Mathieu Frenette
Summary: Raman microscopy can reveal compound-specific vibrational fingerprints without sample preparation. The combination of efficient theoretical calculations and a user-friendly software can accurately predict peak positions and provide match scores to assist with structure determination.
Article
Chemistry, Multidisciplinary
Jayoh A. Hernandez, Paul S. Micus, Sean Alec Lois Sunga, Luca Mazzei, Stefano Ciurli, Gabriele Meloni
Summary: Essential trace metals play crucial roles in the survival and virulence of bacterial pathogens. Helicobacter pylori requires nickel for colonization and persistence in the stomach, and NixA is an essential nickel transporter in this process. This study characterizes the selectivity and electrogenic nature of NixA-mediated nickel transport.
Article
Chemistry, Multidisciplinary
Tarali Devi, Kuheli Dutta, Jennifer Deutscher, Stefan Mebs, Uwe Kuhlmann, Michael Haumann, Beatrice Cula, Holger Dau, Peter Hildebrandt, Kallol Ray
Summary: This study emphasizes the importance of subtle electronic changes and secondary interactions in the stability of biologically relevant metal-dioxygen intermediates. It also shows that the role of the chloride ligand in stabilizing the Fe-III-(OOBu)-Bu-t moiety can extend to other anions, including the thiolate ligand.
Article
Chemistry, Multidisciplinary
Jacqueline R. Santhouse, Jeremy M. G. Leung, Lillian T. Chong, W. Seth Horne
Summary: By studying the folding kinetics and mechanism of the BdpA sequence, researchers found that altering the backbone connectivity can affect protein folding. This suggests that protein mimetic chains have a significant degree of plasticity in transitioning between unfolded and folded states.
Article
Chemistry, Multidisciplinary
Divanshu Gupta, Ralf Einholz, Holger F. Bettinger
Summary: This study presents the first direct spectroscopic evidence of a cyclic seven-membered iminoborane. Compared to linear amino-iminoboranes, this cyclic iminoborane exhibits weakened bond strength and lower Lewis acidity value. The study suggests that the reduced ring strain of cyclic iminoborane prevents nitrogen fixation but allows facile (2 + 2) cycloaddition reaction with C2H4.
Article
Chemistry, Multidisciplinary
Renny Mathew, Aniruddha Mazumder, Praveen Kumar, Julie Matula, Sharmarke Mohamed, Petr Brazda, Mahesh Hariharan, Brijith Thomas
Summary: This study reveals the packing arrangement of partially disordered nitro-perylenediimide (NO2-PDI) using a synergistic approach that combines 3D ED, ssNMR, and DFT techniques. By overcoming these challenges, this methodology opens up new avenues for material characterization, driving exciting advancements in the field.