Article
Biochemistry & Molecular Biology
Haifei Wang, Peng Hui, Yoshinobu Uemoto, Yueyun Ding, Zongjun Yin, Wenbin Bao
Summary: This study investigated the metabolic and proteomic characteristics of PEDV-infected porcine intestinal epithelial cells using liquid chromatography tandem mass spectrometry and isobaric tags for relative and absolute quantification techniques. Changes in metabolism related to cysteine and methionine metabolism, glycine, serine, and threonine metabolism, and mineral absorption were observed. The betaine-homocysteine S-methyltransferase (BHMT) gene was found to potentially regulate these processes and knockdown of BHMT decreased PEDV replication and virus titers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Mingxia Li, Liping Zhang, Li Pan, Peng Zhou, Ruiming Yu, Zhongwang Zhang, Jianliang Lv, Huichen Guo, Yonglu Wang, Sa Xiao, Xinsheng Liu
Summary: In this study, the researchers found that nicotinamide (NAM) effectively inhibits the replication of PEDV and PDCoV by primarily targeting their RNA and protein synthesis processes. The activation of the ERK1/2/MAPK pathway and downregulation of transcription factors also contribute to the inhibition of virus replication. This suggests that NAM could be a promising antiviral candidate not only for swine intestinal coronaviruses but also for other human and animal coronaviruses.
Article
Microbiology
Yingshuo Sun, Ting Gong, Dongdong Wu, Yongzhi Feng, Qi Gao, Jiabao Xing, Xiaoyu Zheng, Zebu Song, Xing Liu, Xiongnan Chen, Yankuo Sun, Guihong Zhang, Lang Gong
Summary: In this study, two strains of PEDV were isolated from diarrheal samples collected from two new PED outbreak farms in 2022. The strains were analyzed for their gene sequences and pathogenicity. The results showed that both strains belong to the G2c subtype and can cause severe diarrhea and intestinal tissue lesions in infected piglets. It was also found that PEDV can be detected in various organs, with the highest levels in the intestinal tissues.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Zhiwei Li, Zhiqian Ma, Linfang Dong, Ting Yang, Yang Li, Dian Jiao, Weiguo Han, Haixue Zheng, Shuqi Xiao
Summary: This study confirms that the S gene is the decisive gene for PEDV adaptability to monkey Vero cells and porcine LLC-PK1 cells. The exchange of S1 and S2 subunits in adaptive strains does not enable PEDV to adapt to cells, and the 803L and 976H residues in the S2 subunit are critical for rCH/SX/2016-S1(HNXP)+S3(HNXP) adaptation to Vero cells. These findings highlight the crucial role of the PEDV S protein in cell tropism and suggest the potential role of coronaviruses S protein in cross-species transmission. Additionally, this study provides unique insights into identifying PEDV functional receptors and developing PEDV and other coronaviruses vaccines.
Article
Immunology
Mingxiang Li, Yiye Zhang, Yuxin Fang, Shaobo Xiao, Puxian Fang, Liurong Fang
Summary: Porcine epidemic diarrhea virus (PEDV) is a re-emerging enteropathogenic coronavirus. In this study, a recombinant trypsin-independent PEDV strain was developed, which can provide protection against both G1 and G2 PEDV infections and does not require trypsin for propagation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Hongyu Rao, Weiwei Su, Xiaoli Zhang, Yonggang Wang, Tao Li, Jinliang Li, Xuan Zeng, Peibo Li
Summary: In this study, Hypericum japonicum extract (HJ) was found to have antiviral effects against PEDV. In vitro experiments showed that HJ could directly inactivate PEDV and inhibit its proliferation at non-cytotoxic concentrations. In vivo experiments demonstrated that HJ could reduce viral titers in the intestines of infected piglets and improve their intestinal pathological, suggesting that HJ has the potential to be developed as an anti-PEDV drug.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Microbiology
Jie Ren, Zhen Ding, Puxian Fang, Shaobo Xiao, Liurong Fang
Summary: PEDV nsp13 is a crucial protein with ATPase and helicase activities that play a key role in unwinding viral RNA. By studying its biochemical properties, new insights have been provided for the development of antiviral drugs.
VETERINARY MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Liting Zhu, Shiguo Liu, Zewen Zhuo, Yanxi Lin, Yanni Zhang, Xiaoling Wang, Lingbao Kong, Ting Wang
Summary: This study demonstrates that the nsp10 protein of PEDV has high immunogenicity and can induce strong antibody responses and cellular immune responses in mice. These findings may have important implications for the future development of PEDV detection and anti-virus drugs for swine.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Jihoon Ryu, Gi-Jong Kang, Onnuri Kim, Jae-Yeon Park, Hyun-Jin Shin
Summary: This study found possible vertical transmission of PEDV through the placenta, which is crucial for controlling the disease.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Hongliang Zhang, Yingguang Li, Ruimei Yang, Ling Xiao, Shaoming Dong, Jiaxu Lin, Gang Liu, Hu Shan
Summary: In this study, it was found that erastin can significantly inhibit the replication of PEDV virus in cells, and it functions by regulating the gene expression of the ferroptosis pathway. This suggests that erastin may be a potential drug for the treatment of PEDV infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Yufang Ge, Feiyang Jiang, Sibei Wang, Heqiong Wu, Yuan Liu, Bin Wang, Wei Hou, Xiuju Yu, Haidong Wang
Summary: During the long-term co-evolution, vaccines may emerge with incomplete protective immunity due to mutations or deletions at specific antigenic sites. Researching and developing new vaccines with cross-protection is important to evaluate current vaccines' efficacy. The prevalence and evolutionary characteristics of PEDV in certain areas of China were examined to contribute to the development of new PEDV vaccines.
Article
Agriculture, Dairy & Animal Science
Baicheng Huang, Guoqian Gu, Yunjing Zhang, Zhenzhen Chen, Kegong Tian
Summary: This study analyzed the complete genomes of 647 strains of the highly contagious porcine epidemic diarrhea virus (PEDV) to understand its evolutionary characteristics. The results showed that the S protein, especially its S1 domain, experienced high positive selective pressure. The S gene was found to be more representative of the evolutionary relationship at the genome-wide level, and the haplotype networks revealed geographical clustering of strains. These findings provide valuable insights into the evolution of PEDV and offer guidance for developing effective prevention measures.
Article
Immunology
Jihee Kim, Jaewon Yoon, Jung-Eun Park
Summary: The study identified the essential role of the M protein in the formation of PED VLPs. Immunization with PED VLPs induced IgG and IgA antibodies in mice, protecting against PED virus infection in Vero cells. PED VLPs immunization also induced Th2-dominant immune responses in mice.
Article
Virology
Wan Li, Mingkai Lei, Zhuofei Li, Huimin Li, Zheng Liu, Qigai He, Rui Luo
Summary: In this paper, a bivalent vaccine against PEDV and PoRV was developed and shown to be effective in preventing infections and improving piglet survival rates.
Article
Infectious Diseases
Mingpu Qi, Corina Zambrano-Moreno, Pilar Pineda, Claudia Calderon, Maria A. Rincon-Monroy, Andres Diaz, Douglas G. Marthaler
Summary: This study investigated the spread of Porcine Epidemic Diarrhea Virus (PEDV) in Colombia and found significant variations in the proportion of positive samples over time. Recombinant regions were identified in 21 strains of PEDV, with phylogenetic analysis revealing 7 lineages with common ancestors. Antigenic analysis showed residue differences in neutralizing epitopes in the spike and nucleocapsid proteins.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)