Article
Virology
Gerson I. Caraballo, Romel Rosales, Mercedes Viettri, Juan Manuel Castillo, Raymundo Cruz, Siyuan Ding, Harry B. Greenberg, Juan E. Ludert
Summary: Dengue is the most important mosquito-borne viral disease to humans. Dengue virus NS1 is a multifunctional protein essential for replication and modulation of innate immunity. The protein interactome of DENV NS1 in mosquito cells was investigated, revealing interactions with various mosquito protein targets. This study provides novel insights into the functions of NS1 and its role in promoting viral replication, as well as identifying new protein pathways involved in mosquito cells.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Jose Angel Rubio-Miranda, Febe Elena Cazares-Raga, Andrea Santos Coy-Arechavaleta, Mercedes Viettri, Leticia Cortes-Martinez, Anel Lagunes-Guillen, Bibiana Chavez-Munguia, Juan E. Ludert, Fidel de la Cruz Hernandez-Hernandez
Summary: Septins are a family of GTP-binding proteins that play roles in cell division, chromosomal segregation, intracellular vesicular traffic, and response to pathogens. This study reveals the involvement of septins in the replication of dengue virus in mosquito cells, highlighting their interaction with viral proteins.
Review
Virology
Romchat Kraivong, Nuntaya Punyadee, M. Kathryn Liszewski, John P. Atkinson, Panisadee Avirutnan
Summary: Dengue is a mosquito-borne viral disease that can lead to significant health and economic burdens globally. The dengue virus has four serotypes, with primary infections often being asymptomatic or causing mild symptoms, while secondary infections with a different serotype can result in severe disease. The complement system and lectin pathway play key roles in the pathogenesis of dengue, with differences in viral glycan recognition influencing the efficiency of virus binding and neutralization.
Article
Pharmacology & Pharmacy
Na Li, Cheng-Lin Deng, Qi Li, Xiao-Ling Chen, Bo Zhang, Han-Qing Ye
Summary: High titers of replication-defective ZIKV with NS1 deletion (ZIKV-Delta NS1) were generated using the NS1 transcomplementation system. ZIKV-Delta NS1 vaccine induced antibody responses and provided protection against ZIKV infection in mice. Moreover, ZIKV-Delta NS1 vaccination attenuated vertical transmission during pregnancy. These findings highlight the potential of the NS1 trans-complementation system for developing flavivirus vaccines.
ANTIVIRAL RESEARCH
(2023)
Article
Virology
Ana C. Alcala, Jose L. Maravillas, David Meza, Octavio T. Ramirez, Juan E. Ludert, Laura A. Palomares
Summary: Dengue is a common viral disease transmitted by mosquitoes. This study reveals that the dengue virus NS1 enters cells through the human HDL receptor, which has significant implications for viral replication and pathogenesis.
JOURNAL OF VIROLOGY
(2022)
Review
Immunology
Kassandra L. Carpio, Alan D. T. Barrett
Summary: The Flavivirus genus includes many important human pathogens, with NS1 protein playing a crucial role as a diagnostic marker for flavivirus infection. Its roles in disease pathogenesis and protective immunity make NS1 an excellent target for candidate vaccines.
Article
Immunology
Nikole L. L. Warner, Susan B. B. Core, Kathryn M. M. Frietze
Summary: Dengue virus is a global health problem and there is a need for more effective vaccines. This study identified peptide epitopes of the non-structural protein 1 of the virus that could induce antibodies capable of binding to infected cells. These peptides may be potential targets for future vaccine development.
Article
Virology
Daed El Safadi, Gregorie Lebeau, Alise Lagrave, Julien Melade, Lauriane Grondin, Sarah Rosanaly, Floran Begue, Mathilde Hoareau, Bryan Veeren, Marjolaine Roche, Jean-Jacques Hoarau, Olivier Meilhac, Patrick Mavingui, Philippe Despres, Wildriss Viranaicken, Pascale Krejbich-Trotot
Summary: By analyzing the EVs produced by infected cells, it was discovered that exosomes are associated with the non-structural protein 1, which may have significant implications in the pathophysiology of Dengue or Zika diseases. Moreover, it was demonstrated that exosomes have the ability to transport NS1, making them a potential tool for vaccine development.
Article
Virology
Nicholas T. N. Lo, Susan Z. Roodsari, Nicole L. Tin, Marcus P. Wong, Scott B. Biering, Eva Harris
Summary: Flavivirus NS1 is a viral protein that is secreted into the bloodstream during an infection. It plays a role in severe dengue pathology such as endothelial dysfunction and vascular leak. In this study, we investigated the molecular determinants that allow some flavivirus NS1 proteins to bind to specific endothelial cells and cause vascular leak in a mouse model. We found that the wing domain of NS1 is a primary determinant for endothelial dysfunction and vascular leak, and narrowed down the contributing amino acid residues to a three-residue motif within the wing domain.
JOURNAL OF VIROLOGY
(2022)
Article
Cell Biology
Sundy N. Y. Yang, Belinda Maher, Chunxiao Wang, Kylie M. Wagstaff, Johanna E. Fraser, David A. Jans
Summary: This study identifies two drugs that can inhibit the replication of dengue virus and other flaviviruses by directly binding to a viral protein.
Article
Virology
Szu-Chia Hsieh, Wen-Yang Tsai, Jih-Jin Tsai, Mars Stone, Graham Simmons, Michael P. Busch, Marion Lanteri, Susan L. Stramer, Angel Balmaseda, Eva Harris, Wei-Kung Wang
Summary: The study highlights the need for better serological tests to distinguish past ZIKV, DENV, and other flavivirus infections, and improve vaccine strategies in endemic regions where interactions between these viruses are critical.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Vinicius Pinho dos Reis, Markus Keller, Katja Schmidt, Rainer Guenter Ulrich, Martin Hermann Groschup
Summary: The absence of alpha V beta 3 integrin significantly impairs the replication of flaviviruses.
Article
Virology
Mafalda A. Farelo, Despoina Korrou-Karava, Katrina F. Brooks, Tiffany A. Russell, Kevin Maringer, Peter U. Mayerhofer
Summary: Flaviviruses such as dengue virus and Zika virus use the host protein PEX19 to downregulate peroxisomes, which results in reduced interferon production. However, unlike cellular peroxisomal membrane proteins, the viral proteins do not localize to peroxisomes. Furthermore, the presence of viral proteins leads to impaired peroxisomal biogenesis through a PEX19-independent mechanism. This challenges the current understanding of how flaviviruses manipulate peroxisomal abundance and suggests an unknown role of peroxisomes in viral biology.
Article
Virology
Michelle Felicia Lee, Mohd Ishtiaq Anasir, Chit Laa Poh
Summary: Dengue infections pose a critical threat to public health worldwide, and there is an urgent need to develop effective antivirals. Peptides designed to target the DENV envelope protein show promise as antiviral candidates. Among the twelve peptides designed, four exhibited potent inhibitory effects against multiple DENV serotypes. Peptide 3 demonstrated the best overall antiviral activity, while peptide 5F showed particular efficacy against DENV-4 during post-infection treatment.
Article
Multidisciplinary Sciences
Linh Tran, Sangkwon Park
Summary: A highly sensitive immunosensor using quantum dots and streptavidin was developed for detecting the dengue biomarker NS1, offering a fast, convenient, and sensitive approach. However, interference in human plasma serum solution was observed, suggesting the need for further solutions.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Eshwar Reddy Tammineni, Arianna Mahely Hurtado-Monzon, Maria Carmen Garcia, Elba Dolores Carrillo, Ascencion Hernandez, Rosa Maria del Angel, Jorge Alberto Sanchez
Summary: The study found that calcium ions and calmodulin play an important role in dengue virus infection of cardiac cells, and the drug dantrolene may help protect against severe viral cardiac morbidity.
Article
Virology
Selvin Noe Palacios-Rapalo, Luis Adrian De Jesus-Gonzalez, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Ana Lorena Gutierrez-Escolano, Rosa Maria del angel
Summary: The NS3 protein of dengue virus is initially present in the nucleus of Huh7 cells during early infection, but later mainly localizes in the cytoplasm.
ARCHIVES OF VIROLOGY
(2021)
Review
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Daniel Cordero-Rivera, Carlos Noe Farfan-Morales, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Various viruses manipulate the integrity of the nuclear pore complex (NPC) to facilitate their replication by altering nucleus-cytoplasmic transport. This alteration is not only observed in viruses with nuclear replicative cycles, but also in viruses with cytoplasmic replication. Viral proteins, particularly viral proteases, play a crucial role in mediating these changes.
Article
Multidisciplinary Sciences
Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Juan Fidel Osuna-Ramos, Irma Eloisa Monroy-Munoz, Luis Adrian De Jesus-Gonzalez, Jose Esteban Munoz-Medina, Arianna M. Hurtado-Monzon, Jose Manuel Reyes-Ruiz, Rosa Maria del Angel
Summary: The study showed that Metformin has a broad antiviral spectrum in vitro, with significant effects in inhibiting Dengue virus infection, but limited effects in inhibiting Zika virus infection. Additionally, in animal models, Metformin can prolong the survival time of mice infected with Dengue virus.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Jose Manuel Reyes-Ruiz, Rosa Campuzano-Vences, Juan Fidel Osuna-Ramos, Luis Adrian De Jesus-Gonzalez, Maria J. Perez-Mendez, Crescencio Gonzalez-Gonzalez, Carlos Noe Farfan-Morales, Leticia Rivas-Tovar, Eduardo Davila-Gonzalez, Rosa Maria del Angel, Aaron P. Gutierrez-Garduno, Enrique Villegas-del Angel, Paola Zarate-Segura, Fernando Bastida-Gonzalez
Summary: This case report describes a 42-year-old female patient with co-infection of COVID-19 and dengue virus, presenting with complex clinical manifestations including liver abnormalities and decreased platelet count. Further research is needed to investigate the impact of SARS-CoV-2 and dengue virus coinfection on hepatic pathology.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Virology
Carlos Daniel Cordero-Rivera, Luis Adrian De Jesus-Gonzalez, Juan Fidel Osuna-Ramos, Selvin Noe Palacios-Rapalo, Carlos Noe Farfan-Morales, Jose Manuel Reyes-Ruiz, Rosa Maria Del Angel
Summary: Flaviviruses are arthropod-borne pathogens responsible for significant mortality and morbidity worldwide. The viral entry process involves multiple steps such as attachment, internalization, endosomal escape, and capsid uncoating, providing potential targets for developing broad-spectrum antiviral drugs targeting host factors and cellular pathways.
CURRENT OPINION IN VIROLOGY
(2021)
Article
Virology
Ana C. Alcala, Jose L. Maravillas, David Meza, Octavio T. Ramirez, Juan E. Ludert, Laura A. Palomares
Summary: Dengue is a common viral disease transmitted by mosquitoes. This study reveals that the dengue virus NS1 enters cells through the human HDL receptor, which has significant implications for viral replication and pathogenesis.
JOURNAL OF VIROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ana C. Alcala, Martha A. Contreras, Esmeralda Cuevas-Juarez, Octavio T. Ramirez, Laura A. Palomares
Summary: Sericin improves Zika virus amplification in insect and mammalian cell cultures and has cryoprotectant capacity.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Microbiology
Selene Zarate, Blanca Taboada, Jose Esteban Munoz-Medina, Pavel Isa, Alejandro Sanchez-Flores, Celia Boukadida, Alfredo Herrera-Estrella, Nelly Selem Mojica, Mauricio Rosales-Rivera, Bruno Gomez-Gil, Angel Gustavo Salas-Lais, Clara Esperanza Santacruz-Tinoco, Hector Montoya-Fuentes, Julio Elias Alvarado-Yaah, Gloria Maria Molina-Salinas, Gloria Elena Espinoza-Ayala, Jose Antonio Enciso-Moreno, Rosa Maria Gutierrez-Rios, Antonio Loza, Joaquin Moreno-Contreras, Rodrigo Garcia-Lopez, Xaira Rivera-Gutierrez, Andreu Comas-Garcia, Rosa Maria Wong-Chew, Maria-Eugenia Jimenez-Corona, Rosa Maria del Angel, Joel Armando Vazquez-Perez, Margarita Matias-Florentino, Marissa Perez-Garcia, Santiago Avila-Rios, Hugo G. Castelan-Sanchez, Luis Delaye, Leon P. Martinez-Castilla, Marina Escalera-Zamudio, Susana Lopez, Carlos F. Arias
Summary: The B.1.1.7 (Alpha) lineage of SARS-CoV-2 emerged and rapidly increased worldwide, but did not become the dominant lineage in most of Mexico. The states at the Mexico-U.S. border had the highest prevalence of B.1.1.7 and showed the highest lineage diversity, suggesting possible introductions from the United States. Community transmission of B.1.1.7 occurred in Mexico, as evidenced by localized distribution and specific mutations.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Wendy Marilu Ramos-Hernandez, Luis F. Soto, Marcos Del Rosario-Trinidad, Carlos Noe Farfan-Morales, Luis Adrian De Jesus-Gonzalez, Gustavo Martinez-Mier, Juan Fidel Osuna-Ramos, Fernando Bastida-Gonzalez, Victor Bernal-Dolores, Rosa Maria del Angel, Jose Manuel Reyes-Ruiz
Summary: This study finds that leukocyte glucose index (LGI) is an independent risk factor for severe COVID-19, especially in female patients and those with diabetes. LGI shows potential as a biomarker for predicting severity in COVID-19 patients, with significant improvements when combined with respiratory rate.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Infectious Diseases
Ruth Blanco, Ana C. Alcala, Rixio Fernandez, Viviana Ramirez, Rita E. Rosales, Maria G. Paez, Hector Aleman, Rosabel Gonzalez, Jose Zerpa, Antonio J. Maldonado, Esmeralda Vizzi
Summary: This study investigated the prevalence and molecular diversity of HAdV-F in Venezuela. The results showed a progressive increase in the detection rate of HAdV in pediatric acute gastroenteritis, with higher rates in children vaccinated against rotavirus. HAdV-F41 was the predominant virus in AGE, and its genomic similarity was observed with global strains.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Article
Virology
Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Luis Adrian De Jesus-Gonzalez, Jose Manuel Reyes-Ruiz, Arianna M. M. Hurtado-Monzon, Selvin Noe Palacios-Rapalo, Ricardo Jimenez-Camacho, Marco Antonio Meraz-Rios, Rosa Maria Del Angel
Summary: This study investigated the antiviral potential of atorvastatin and ezetimibe in monotherapy and combination against Dengue, Zika, and Yellow Fever viruses. The in vitro results showed that both drugs could reduce the percentage of infected cells in a dose-dependent manner. The combination of atorvastatin and ezetimibe exhibited synergistic effects against Dengue 2, additive effects against Dengue 4 and Zika, and antagonistic effects against Yellow Fever. In mice infected with Dengue 2, monotherapy with atorvastatin or ezetimibe improved clinical signs and increased survival. However, the combination of both drugs did not significantly affect survival. This study provides valuable insights into the potential of atorvastatin and ezetimibe as antiviral agents against flaviviruses and highlights the need for further investigations into their combined therapeutic effects.
Article
Microbiology
Aurora Montsserrat Gonzalez-Flores, Mariana Salas-Benito, Victor Hugo Rosales-Garcia, Paola Berenice Zarate-Segura, Rosa Maria Del angel, Monica Ascencion De Nova-Ocampo, Juan Santiago Salas-Benito
Summary: Arboviruses are important pathogens causing diseases worldwide. Viral interference is a complex phenomenon where the presence of two different viruses in the same cell inhibits replication of one virus. In this study, a mosquito cell line persistently infected with Dengue virus was used to study viral interference. The results suggest that viral interference may involve small RNAs and occurs independently of the Ago3 pathway.
Article
Infectious Diseases
Juan Fidel Osuna-Ramos, Jose Manuel Reyes-Ruiz, Luis Antonio Ochoa-Ramirez, Luis Adrian De Jesus-Gonzalez, Rosalio Ramos-Payan, Carlos Noe Farfan-Morales, Alejandra Romero-Utrilla, Efren Rafael Rios-Burgueno, Jose Rodriguez-Millan, Rosa Maria del Angel, Jesus Salvador Velarde-Felix
Summary: This study evaluated the accuracy of peripheral blood cell count in distinguishing between dengue and COVID-19. The results showed that neutrophil, platelet counts, and NLR or NLPR can be used as markers to differentiate between the two diseases, providing a quick and cost-effective way for diagnosis in low-income tropical regions with co-epidemics.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
