期刊
VIROLOGY
卷 496, 期 -, 页码 138-146出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2016.06.001
关键词
Influenza; PA-X; Equine H3N8; Canine H3N8; Gene suppression; RNA-seq
类别
资金
- NSF [2012141647]
- NHMRC Australian Fellowship [AF30]
- National Institutes of Health (NIH) [R01 GM8496821]
The influenza PA-X protein is translated from the PA open reading frame from frameshifting and suppresses cellular gene expression due to its ribonuclease activity. We further defined the functional roles of PA-X by comparing PA-X proteins from two related viruses - equine influenza (EIV) and canine influenza (CIV) H3N8 - that differ in a C-terminal truncation and internal mutations. In vitro reporter gene assays revealed that both proteins were able to suppress gene expression. Interestingly, EIV PA-X demonstrated similar to 50% greater activity compared to CIV PA-X, and we identified the mutations that caused this difference. We used RNA-seq to evaluate the effects of PA-X on host gene expression after transfection into cultured cells. There were no significant differences in this property between EIV and CIV PAX proteins, but expression of either resulted in the up-regulation of genes when compared to controls, most notably immunity-related proteins, trafficking proteins, and transcription factors. (C) 2016 Elsevier Inc. All rights reserved.
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