期刊
VIBRATIONAL SPECTROSCOPY
卷 85, 期 -, 页码 112-121出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vibspec.2016.04.007
关键词
Antibacterial; FTIR spectroscopy; Silver; Silver ion; Silver nanoparticles; Escherichia coli.
资金
- National Natural Science Foundation of China [21075027]
- Natural Science Foundation of Hebei Province [B2011201082, B2016201034]
- Key Project of Chinese Ministry of Education [211014]
- Research Fund for the Doctoral Program of Higher Education of China [20121301110003]
In this study, we performed the first comparative study of the antibacterial mechanisms of silver ion (Ag+) and silver nanoparticles (AgNPs) on Escherichia coli (E. coli) using Fourier transform infrared (FTIR) spectroscopy. Through a thorough analysis of the FTIR spectra of E. coli after silver treatment in the spectral regions corresponding to thiol group, protein, lipopolysaccharide (LPS), and DNA, we were able to reveal a multifaceted antibacterial mechanism of silver at the molecular level for both Ag+ and AgNPs. Features of such mechanism include: (1) silver complexes with thiol group; (2) silver induces protein misfolding; (3) silver causes loss of LPS from bacterial membrane; (4) silver changes the overall conformation of DNA. Despite the similarities between Ag+ and AgNPs with respect to their antibacterial mechanisms, we further revealed that Ag+ and AgNPs display quite different kinetics for silver-thiol complexation and loss of LPS, with Ag+ displaying fast kinetics and AgNPs displaying slow kinetics. At last, we proposed a hypothesis to interpret the observed different behaviors between Ag+ and AgNPs when interacting with E. coli. (C) 2016 Elsevier B.V. All rights reserved.
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