Article
Microbiology
Marta Dec, Dagmara Stepien-Pysniak, Klaudiusz Szczepaniak, Barbara Turchi, Renata Urban-Chmiel
Summary: Exotic reptiles are increasingly being bred as pets, but close contact between reptiles and their owners can lead to the transmission of zoonotic pathogens. This study examined E. coli isolates from captive reptiles, revealing that the incidence of E. coli was low but strains carried virulence genes and had the potential to cause disease. Therefore, good hygiene practices should be followed when in contact with reptiles.
Article
Infectious Diseases
Momna Rubab, Deog-Hwan Oh
Summary: The study investigated the antibiotic resistance of Shiga toxin-producing Escherichia coli (STEC) isolates, finding that most strains exhibited phenotypic resistance, with common resistant genes including ere(A), aadA1, and aac(3)-I. These results underscore the importance of monitoring multidrug resistance globally.
Article
Environmental Sciences
Liping Ma, Huiying Yang, Lei Guan, Xiaoyu Liu, Tong Zhang
Summary: Chlorination process can significantly decrease both abundance and diversity of total ARGs, but with limited removal rates for opportunistic pathogens, such as E. coli isolates. Core subtypes of ARGs were identified and found to persist during chlorination for both total microbes and E. coli. Additionally, metagenomic binning strategies revealed enrichment of certain ARG-carrying genomes after chlorination.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Agriculture, Dairy & Animal Science
Marcin Smialek, Joanna Kowalczyk, Andrzej Koncicki
Summary: Escherichia coli infections are a major cause of economic loss in the poultry industry, and vaccination against E. coli has a positive impact on bird health without affecting vaccination efficacy against other poultry diseases. The study conducted three independent experiments demonstrating that vaccination reduces E. coli population counts and increases antibiotic susceptibility in field isolates without impacting vaccination against another major poultry disease, TRT.
Article
Agriculture, Dairy & Animal Science
Luisa Massaccesi, Elisa Albini, Francesca Romana Massacci, Serenella Orsini, Silvia Tofani, Francesca Blasi, Lucia Marchi, Giovanni Pezzotti, Chiara Francesca Magistrali
Summary: This study evaluated the load and prevalence of antibiotic-resistant commensal E. coli along the goose production cycle on two free-range farms in central Italy. Differences in bacterial loads were observed between the farm that administered oxytetracycline and the one that did not use antibiotics. The dynamics of antibiotic-resistant E. coli in farmed geese are similar to those observed in broilers, which may be mitigated by adopting good management practices, including prudent use of antibiotics.
Article
Multidisciplinary Sciences
Jude Fonbah Leinyuy, Innocent Mbulli Ali, Karimo Ousenu, Christopher B. Tume
Summary: This study analyzed 200 Enterobacteriaceae isolates resistant and susceptible to ESBL, quinolones, and aminoglycosides. It found a high carriage of resistance genes, but the carriage of resistance genes may not always be correlated with phenotypic expression.
Article
Biochemistry & Molecular Biology
Ivica Samanic, Blanka Dadic, Zeljka Sanader Marsic, Mia Dzelalija, Ana Maravic, Hrvoje Kalinic, Pavle Vrebalov Cindro, Zeljko Sundov, Marija Tonkic, Ante Tonkic, Jonatan Vukovic
Summary: This study investigated the molecular genetic mechanisms of clarithromycin and levofloxacin resistance in Helicobacter pylori infections in patients from Croatia. Point mutations in the 23S rRNA, gyrA, and gyrB genes were found to alter target sites or protein structure, leading to reduced efficacy of standard antibiotics. Molecular docking analysis revealed that resistance-related mutations resulted in decreased susceptibility to clarithromycin and levofloxacin due to significant differences in non-covalent interactions, ultimately resulting in antibiotic resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Hamideh Richi Sharabiani, Javid Sadeghi, Tahere Pirzade, Mohammad Ahangarzadeh Rezaee, Reza Ghotaslou, Delara Laghousi, Fateme Yeghane Sefidan, Hossein Samadi Kafil, Mojtaba Nikbakht, Fariba Naeimi Mazraeh, Yalda Hematyar
Summary: The study revealed a high concordance rate between colonizing and clinical isolates of S. aureus in terms of adhesion factors and superantigen genes. Nasal decolonization is suggested to be effective in preventing S. aureus infections.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Dalmasso Guillaume, Beyrouthy Racha, Brugiroux Sandrine, Ruppe Etienne, Guillouard Laurent, Bonnin Virginie, Saint-Sardos Pierre, Ghozlane Amine, Gaumet Vincent, Barnich Nicolas, Delmas Julien, Bonnet Richard
Summary: Without the use of antibiotics, the plasmid-mediated resistance gene mcr-1 enhances the colonization ability of Escherichia coli in the gut, but impairs its lethal effect. This improvement in gut fitness is associated with a downregulation of intestinal inflammatory markers and the preservation of intestinal microbiota composition.
Article
Infectious Diseases
Chia-Hung Tsai, Yu-Chin Chen, Po-Yu Chen, Chih-Cheng Lai, Hung-Jen Tang, Yin-Ching Chuang, Chi-Chung Chen, Chung-Han Ho, Wei-Yun Hsu, Tu-Hsuan Chang
Summary: This study investigated the antibiotic susceptibility of Escherichia coli isolates in patients diagnosed with intra-abdominal infections in the Asia-Pacific region. The results showed that community-associated E. coli isolates generally had higher susceptibility rates for most antibiotics compared to hospital-associated isolates. Ceftriaxone and piperacillin/tazobactam exhibited reduced susceptibility rates in certain countries.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Biotechnology & Applied Microbiology
Amir Mirzaie, Reza Ranjbar
Summary: This study investigated antibiotic resistance, virulence-associated genes analysis, and molecular typing of Klebsiella pneumoniae strains. The findings revealed a high prevalence of resistance, presence of various virulence factors, and high level of efflux pump and biofilm gene expression in different clones of K. pneumoniae strains, posing a significant health issue in clinical settings.
Article
Pediatrics
Razieh Shahbazi, Siavosh Salmanzadeh-Ahrabi, Mohammad Mehdi Aslani, Masoud Alebouyeh, Jamal Falahi, Vajihe Sadat Nikbin
Summary: This study investigated the prevalence, clonality, virulence capacity, and antibiotic resistance phenotype and genotype of E. coli O25-B2-ST131 in children with urinary tract infection in Tehran, Iran. The results showed a higher frequency of antibiotic resistance and ESBLs production in O25-B2-ST131 strains compared to non-O25-B2-ST131 strains. The study confirmed the involvement of MDR-ESBLs producing E. coli strain O25-B2-ST131 in the occurrence of UTIs among children and highlighted the need for source tracking and control measures.
Article
Biochemistry & Molecular Biology
Nabi Jomehzadeh, Morteza Saki, Khadijeh Ahmadi, Golshan Zandi
Summary: This study aimed to determine the prevalence of quinolone resistance and the presence of plasmid-mediated quinolone resistance (PMQR) genes among extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates. The results showed a high resistance rate to nalidixic acid and ciprofloxacin among ESBL-producing isolates. The most frequent PMQR gene was aac(6')-Ib-cr variant. The coexistence of aac(6')-Ib-cr variant and qnrB was the most widely distributed genotype among quinolone resistance isolates.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Infectious Diseases
Marwa Atef Yakout, Ghada Hani Ali
Summary: This study investigated the mutations in gyrA and parC genes as well as the acquisition of qnrA and qnrB genes. The results showed that the most common mutation in the gyrA gene was ser83leu, followed by asp87asn and lys154arg. The most detected mutation in the parC gene was ser80ile. Other mutations, including a substitution mutation at codon 157, were also observed. The qnrB gene was the most commonly detected, with 64.7% of Klebsiella pneumoniae and 57.1% of Escherichia coli being positive. The qnrA gene was detected in 11% of Klebsiella pneumoniae and 4% of Escherichia coli isolates tested.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2022)
Article
Microbiology
Dimard E. Foudraine, Lennard J. M. Dekker, Nikolaos Strepis, Stan J. Nispeling, Merel N. Raaphorst, Wendy Kloezen, Piet Colle, Annelies Verbon, Corne H. W. Klaassen, Theo M. Luider, Wil H. F. Goessens
Summary: A multiplex-targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay was developed and validated for the rapid and accurate detection of resistance mechanisms in Escherichia coli or Klebsiella pneumoniae complex from positive blood cultures. The assay showed high agreement with whole-genome sequencing results and correctly predicted the majority of susceptible and non-susceptible phenotypes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Environmental Sciences
Kaja Rola, Anna Lenart-Boron, Piotr Boron, Piotr Osyczka
Summary: This study reveals the genetic and anatomical diversity of Asterochloris photobionts in different lichens, showing their ability to tolerate heavy-metal pollution. The mycobionts demonstrate low selectivity and can associate with various Asterochloris lineages, indicating adaptability. The photobiont characteristics may be closely dependent on heavy-metal pollution, with certain lineages showing superior adaptation to polluted sites.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biology
Maria Chmiel, Anna Lenart-Boron
Summary: This study aimed to investigate the changes in bacterial and fungal aerosol levels in historical objects in Krakow, as well as the factors influencing air quality using data mining techniques. The results showed that the concentrations of bacterial and fungal aerosols were not always correlated with particulate matter levels, with temperature and relative humidity playing a more significant role in shaping microbial aerosol levels.
Article
Microbiology
Martin S. Mullett, Rein Drenkhan, Kalev Adamson, Piotr Boron, Anna Lenart-Boron, Irene Barnes, Michal Tomsovsky, Zuzana Janosikova, Katarina Adamcikova, Emilia Ondruskova, Valentin Queloz, Barbara Piskur, Dmitry L. Musolin, Kateryna Davydenko, Margarita Georgieva, Sophie Schmitz, Audrius Kacergius, Luisa Ghelardini, Jelena Kranjec Orlovic, Michael Muller, Funda Oskay, Tine Hauptman, Agnes Halasz, Svetlana Markovskaja, Halvor Solheim, Martti Vuorinen, Renate Heinzelmann, Richard C. Hamelin, Adam Konecny
Summary: The study revealed the global origin and migration pathways of Dothistroma septosporum, indicating its native range to be in Eastern Europe and Western Asia, with Eastern Europe having highly diverse populations.
Article
Agronomy
Piotr Boron, Anna Lenart-Boron, Martin Mullett, Bartlomiej Grad, Katarzyna Nawrot-Chorabik
Summary: The population of D. septosporum in Poland is highly structured, with specific population clusters identified in different regions. Multiple independent introduction events/migration waves were observed, suggesting a single introduction, likely human-mediated, at the original outbreak site, and at least two subsequent waves representing a general shift in distribution as a result of natural spread.
Article
Chemistry, Physical
Karen Khachatryan, Lusine Khachatryan, Marcel Krzan, Magdalena Krystyjan, Lidia Krzeminska-Fiedorowicz, Anna Lenart-Boron, Aneta Koronowicz, Mariola Drozdowska, Gohar Khachatryan
Summary: This study prepared foils of pure sodium hyaluronate and sodium hyaluronate containing nanosilver, graphene oxide, nanosilver/graphene oxide, and characterized their properties. The addition of nanoparticles caused changes in molecular weights in the samples. The foils containing nanosilver and nanosilver/graphene oxide exhibited bacteriostatic activity and cytotoxic effects on human melanoma cell lines.
Article
Environmental Sciences
Anna Lenart-Boron, Dagmara Drab, Justyna Chrobak
Summary: The study assessed bioaerosol, particulate matter concentration, and antibiotic resistance of airborne Staphylococcus in various premises of Poland's oldest agricultural school. Different levels of bioaerosol were found between sampling points, with the highest levels in the newborn calf shed. Particulate matter concentrations were lowest in school rooms and highest in the newborn calf shed, but did not exceed workplace threshold values. The most prevalent Staphylococcus species were S. equorum, S. succinus, and S. xylosus, with high resistance to macrolides and lincosamides observed.
Article
Chemistry, Physical
Milosz Rutkowski, Lidia Krzeminska-Fiedorowicz, Gohar Khachatryan, Julia Kabacinska, Marek Tischner, Aleksandra Suder, Klaudia Kulik, Anna Lenart-Boron
Summary: This study successfully synthesized biodegradable silver nanoparticle-containing foils and confirmed their antibacterial activity against bacterial pathogens isolated from the oral cavities of cats, dogs, and horses. The size of the silver nanoparticles varied depending on the reducing agent used, but all types of examined foils were effective against the isolated bacteria, including E. coli, ESBL, S. aureus, and MRSA.
Article
Chemistry, Multidisciplinary
Anna Lenart-Boron, Anna Bajor, Marek Tischner, Klaudia Kulik, Julia Kabacinska
Summary: This study provides scientific evidence for improving the welfare of horses in stables and finding ways to eliminate the causal agents of recurrent airway disease. It suggests that regular examinations of particulate matter and fungal aerosol concentrations, along with species composition assessment, should be conducted in horse stables.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Gohar Khachatryan, Lusine Khachatryan, Magdalena Krystyjan, Anna Lenart-Boron, Marcel Krzan, Klaudia Kulik, Anna Bialecka, Maja Grabacka, Nikola Nowak, Karen Khachatryan
Summary: In this study, an innovative hydrogel containing micro/nanocapsules of ozonated olive oil based on hyaluronic acid was developed. The hydrogel exhibited regenerative properties and a weak inhibitory effect against bacterial commensal skin microbiota and pathogenic Candida-like yeasts. The results also showed that the emulsions were rheologically stable, exhibited an antimicrobial effect, and were non-toxic in the HaCat keratinocyte model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Ewa Dacewicz, Anna Lenart-Boron
Summary: This study assessed the effectiveness of waste polyurethane sponge materials as biomass carriers in treating domestic sewage with increased ammonium nitrogen content. Comparative experiments were conducted to evaluate different carriers and the microbial community inhabiting the spongy material was characterized. Results showed that Nitrosomonas and Nitrospira were the predominant nitrifiers in the waste polyurethane foams, and unidentified Planctomycetes were responsible for high removal of N-NH4+. Dominant denitrifying bacteria involved in the treatment of ammonium nitrogen-enriched sewage were identified as Burkholderia and Sphingopyxis phyla.
Article
Chemistry, Multidisciplinary
Anna Lenart-Boron, Anna Bojarczuk, Miroslaw Zelazny
Summary: This study examined the impact of the construction and operation of a new ski slope on nearby stream waters. It found that during the construction period, there were significant changes in electrolytic conductivity, pH, chemical substances, and bacteria. However, after construction, some indicators returned to their pre-construction state, possibly due to vegetation recovery and water self-purification. Thus, conducting impact assessments prior to new ski slope construction is crucial.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Anna Lenart-Boron, Justyna Prajsnar, Maciej Guzik, Piotr Boron, Bartlomiej Grad, Miroslaw Zelazny
Summary: This study investigated the presence and concentration of antibiotics in surface water and groundwater in southern Poland, revealing spatial and temporal trends in antibiotic occurrence along the Bialka river valley, with sewage treatment plant effluent identified as the main source of antibiotic contamination downstream. The detection rates of antibiotics in the examined region appear to be primarily driven by the stability of antibiotics in the environment.
APPLIED SCIENCES-BASEL
(2022)
Article
Polymer Science
Gohar Khachatryan, Karen Khachatryan, Magdalena Krystyjan, Lidia Krzeminska-Fiedorowicz, Anna Lenart-Boron, Anna Bialecka, Magdalena Krupka, Marcel Krzan, Karolina Blaszynska, Monika Hanula, Leslaw Juszczak
Summary: The dynamic development of nanotechnology has led to the innovation and improvement of nanocapsules based on biodegradable biopolymer composites. These nanocapsules gradually release biologically active substances with antimicrobial activity, which have regular, prolonged and targeted effects on pathogens. The antimicrobial properties of the obtained nanocomposites were strong against various bacterial and yeast strains isolated from different regions of the human body, indicating their high potential applicability as effective materials for dressings on infected wounds.
Review
Environmental Sciences
Klaudia Kulik, Anna Lenart-Boron, Kinga Wyrzykowska
Summary: Environmental aquatic pollution with antibiotics is a global challenge, including pristine mountain environments. Monitoring antibiotic concentrations in water is crucial for water resource management. This review focuses on sources and degradation routes of antibiotics in surface waters, particularly in mountains and pristine areas. Pollution is strongly linked to anthropogenic pressure from intensive tourism. The presence of antibiotics at sub-inhibitory concentrations affects bacterial populations and drives microbial evolution, impacting ecological functions and drug resistance. Stability and half-lives of antibiotics in water, influenced by molecular structures and environmental conditions, are important considerations. Wastewater treatment, especially advanced techniques, can effectively remove antibiotics and enable safe reuse of wastewater. Current research on antibiotic emergence in mountain areas and knowledge gaps are discussed.
Article
Mycology
Piotr Osyczka, Anna Lenart-Boron, Piotr Boron, Kaja Rola
Summary: Lichens demonstrate strong selectivity in choosing their algal partners, with the biological properties of the photobiont significantly affecting their habitat requirements. The flexibility in photobiont choice expands the ecological range of lichens and may serve as an important adaptive strategy for colonization of extreme habitats. The presence of specific algae in lichen species is influenced by habitat type and levels of soil pollution, with potential alternative photosynthetic partners like Trebouxia adapting well to metal pollution.
