Article
Immunology
Shuaijuan Han, Yang Wen, Fengfan Yang, Pingli He
Summary: Chicken egg yolk antibody (IgY) has been proven to inhibit the growth of enterotoxigenic Escherichia coli (ETEC) and protect against ETEC infection in mice by reducing colonization, improving intestinal health, and modulating immune response. High and medium doses of anti-ETEC IgY demonstrated more effective protective effects against ETEC infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Alberto Prieto, Cynthia Lopez-Novo, Pablo Diaz, Jose Manuel Diaz-Cao, Gonzalo Lopez-Lorenzo, Claudia Anton, Susana Remesar, David Garcia-Dios, Ceferino Lopez, Rosario Panadero, Pablo Diez-Banos, Patrocinio Morrondo, Gonzalo Fernandez
Summary: Neonatal calf diarrhoea is a significant issue in cattle production worldwide, with enterotoxigenic Escherichia coli (ETEC) being one of the major pathogens. Veterinary surgeons commonly use antimicrobials to treat the disease, but this study found that all ETEC strains were resistant to three or more families of antimicrobials. Only a few antibiotics showed efficacy against most ETEC strains, while many strains were resistant to first-line antimicrobials used in veterinary practice. The study emphasizes the importance of performing antimicrobial susceptibility tests in each outbreak to ensure effective treatment and prevent the emergence of new resistance mechanisms.
Article
Agriculture, Dairy & Animal Science
A. Karamzadeh-Dehaghani, A. Towhidi, M. Zhandi, N. Mojgani, A. Fouladi-Nashta
Summary: The study demonstrates that feeding a combination of probiotics and ETEC K99-specific antibody to neonatal Holstein calves can enhance feed efficiency, boost immunity, and reduce diarrhea prevalence.
Article
Chemistry, Applied
Wanyi Dong, Chaoqing Tang, Minquan Xia, Long Sheng, Zhaoxia Cai
Summary: The egg yolk immunoglobulin loaded chitosan-liposomes prepared with the assistance of supercritical carbon dioxide showed better stability and high encapsulation efficiency, serving as an effective carrier to enhance human immunity.
Article
Biochemistry & Molecular Biology
Yuankang Zhou, Yuheng Luo, Bing Yu, Ping Zheng, Jie Yu, Zhiqing Huang, Xiangbing Mao, Junqiu Luo, Hui Yan, Jun He
Summary: The study found that dietary beta-glucan (BGL) supplementation has a protective effect on intestinal epithelium exposed to enterotoxigenic Escherichia coli (ETEC). BGL supplementation improves intestinal absorption, reduces inflammation, enhances intestinal immunity and antioxidant capacity, and improves intestinal microbiota.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Jiameng Hu, Junlin Li, Xiaobo Huang, Jing Xia, Min Cui, Yong Huang, Yiping Wen, Yue Xie, Qin Zhao, Sanjie Cao, Likou Zou, Xinfeng Han
Summary: The study identified and characterized 19 enterotoxigenic Escherichia coli (ETEC) isolates from diarrheic pigs in China. The isolates showed high resistance to multiple antibiotics and carried various virulence factors. Genetic diversity was observed among the isolates, with certain serotypes being dominant. Hybrid ETEC/STEC isolates carrying Shiga-like toxin-related virulence factors were also identified. The presence of plasmids carrying antimicrobial resistance genes highlighted their role in the emergence of hybrid pathotypes. These findings emphasize the importance of controlling and intervening in porcine-origin ETEC strains to mitigate the impact on animal and human health.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Immunology
Kwangwook Kim, Minho Song, Yanhong Liu, Peng Ji
Summary: Enterotoxigenic Escherichia coli (ETEC) infection-induced post-weaning diarrhea is a major health issue in newly weaned pigs, leading to the overuse of antimicrobial drugs. Nutritional intervention is an effective alternative practice to reduce reliance on antibiotics.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Cynthia Jinno, Xunde Li, Yanhong Liu
Summary: This study aimed to characterize the effects of ETEC infection, supplementing B. subtilis DSM 25841, or carbadox on intestinal microbiota of pigs. The results indicated that AGP reduced the relative abundance of beneficial bacteria in the intestines, while DFM had higher relative abundance of beneficial bacteria in some intestinal segments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Cynthia Jinno, Kwangwook Kim, Braden Wong, Emma Wall, Ravichandran Sripathy, Yanhong Liu
Summary: The objective of this study was to investigate the effects of supplementation of botanical blends (BB) on the gut microbiota and metabolomic profiles in pigs infected with Escherichia coli. The results showed that supplementing 100 ppm BB1 increased the relative abundances of Enterobacteriaceae and Escherichia-Shigella in the ileum, and the relative abundances of Bacteroidota and Prevotellaceae in the cecum. Supplementing 100 ppm BB2 upregulated serum pinitol on day 4 post-inoculation and serum cholesterol and aminomalonic acids on day 21 post-inoculation. Addition of 50 ppm BB2 reduced asparagine in the ileal mucosa on day 5 post-inoculation.
Article
Microbiology
Djamel Drider, Rabah Boukherroub, Laetitia Le Devendec, Yanath Belguesmia, Noura Hazime, Gwenaelle Mourand, Frederic Paboeuf, Isabelle Kempf
Summary: This study evaluated the therapeutic efficacy of colistin-loaded alginate nanoparticles in the treatment of post-weaning diarrhoea. The results showed that the nanoparticles had a slightly better therapeutic effect compared to free colistin. However, further studies are needed to evaluate the efficacy of this novel drug in different settings.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Leli Wang, Yuwei Wu, Juan Xu, Qiuyun Huang, Ying Zhao, Sheng Dong, Xuxiang Wang, Xiaoni Cao, Chuni Wang, Anqi Wu, Diao Zhou, Cang Chen, Huansheng Yang, Jianzhong Li, Papadimitriou Konstantinos, Qiang Tu, Gaihua Zhang, Jia Yin
Summary: This study revealed the heterogeneity of E. coli strains from pigs, focusing on two strains studied in detail through both in silico and wet experiments and 14 strains analyzed through bioinformatics analysis. E. coli Y18J may enhance pig adaptability to disease resistance by producing colicins B and M. These findings offer insights into the roles of E. coli in modern animal husbandry, contributing to a better understanding of intestinal-microbe-pathogen interactions during evolution.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Shan-Shan Peng, Yingjie Li, Qiuhong Chen, Qi Hu, Ying He, Lianqiang Che, Ping-Ping Jiang
Summary: This study investigated the changes in mucosal microbiome and protein expression in the ileum induced by ETEC infection using pigs as a model. The results showed that ETEC infection caused inflammation, impaired bile acid circulation, and the mucosal microbiome may affect the expression of intestinal proteins.
Article
Biochemistry & Molecular Biology
Haixiu Wang, Eric Cox, Bert Devriendt
Summary: Enterotoxigenic Escherichia coli (ETEC) strains recognize and respond to factors secreted by gut epithelial cells, leading to transcriptional modulation of key ETEC virulence genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Xi Lu, Dingyan Wu, Xin Zhao, Mingxin Zhang, Ke Ren, Ningning Zhou, Yanni Zhao, Weisheng Qian
Summary: Enterotoxigenic Escherichia coli (ETEC), a diarrheal pathogen, can utilize ethanolamine (EA) as a nitrogen source, which enhances its virulence phenotype and causes damage to host intestinal cells. The concentration of EA in the intestine also impacts the metabolic profile and pathogenicity of ETEC.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Pedro G. Castelo, Lucas A. Rodrigues, Michele de P. Gabardo, Roberto M. Carvalho Guedes, Andrea M. Moreno, Fernanda M. Coura, Marcos Bryan Heinemann, Bruno O. Rosa, Ana P. L. Brustolini, Itallo C. S. Araujo, Dalton de O. Fontes
Summary: This study demonstrates that adding spray-dried plasma (SDP) to post-weaning diets can improve growth performance and reduce bacterial shedding in pigs.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2023)
Article
Polymer Science
Silvestre Bongiovanni Abel, Maria Molina, Claudia R. Rivarola, Cesar A. Barbero
Summary: Micrometric Pickering emulsions stabilized with water-dispersible electroactive polyaniline nanoparticles were studied, showing that the nanoparticles retain their functional properties in the emulsions. Smart Pickering emulsions stabilized with PANI-NP and a thermosensitive polymer were successfully obtained, and their thermal behavior under RF irradiation was evaluated, demonstrating a significant local temperature increase and emulsion breaking.
JOURNAL OF APPLIED POLYMER SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Candelaria Tejada-Tovar, Diofanor Acevedo, Angel Villabona-Ortiz, Norida Pajaro-Gomez, Math Otero
Summary: The research evaluated the adsorption capacity of lemon and cassava peels for Ni (II), finding that they can effectively remove metal contaminants in synthetic aqueous solutions. The best adsorbent particle sizes for lemon and cassava peel were 0.5 mm and 1 mm, respectively, at a pH of 6.
Article
Polymer Science
Silvestre Bongiovanni Abel, Maria Martinez, Mariano M. Bruno, Cesar A. Barbero, Gustavo A. Abraham, Diego F. Acevedo
Summary: In this study, a modular sensor platform was constructed by combining carbonized poly(acrylonitrile) electrospun mat and poly(N-isopropylacrylamide) hydrogel for portable electrochemical sensing. The platform showed promising performance with a detection limit of 0.103 mu mol L-1 for iron sensing. The results indicated a synergistic combination of large surface area carbon fiber electrodes and ion retaining hydrogel for improved electrochemical sensing applications.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2021)
Article
Nanoscience & Nanotechnology
J. Y. Del C. Pereyra, C. A. Barbero, D. F. Acevedo, E. Yslas
Summary: This study reports the antibacterial activity of a nanocomposite made of zinc oxide nanoparticles dispersed in a poly(acrylamide-co-hydroxyethylmethacrylate) matrix against Pseudomonas aeruginosa. The in situ synthesis of ZnONPs inside the crosslinked network of PAAm-Hema is described and the physicochemical properties of the nanocomposite are analyzed. The results show that the nanocomposite has the potential to be used as an antimicrobial agent in biomedical applications.
Article
Pharmacology & Pharmacy
Maria Laura Soriano Perez, Javier Alejandro Funes, Carolina Flores Bracamonte, Luis Exequiel Ibarra, Marina Andrea Forrellad, Oscar Taboga, Laura Noelia Cariddi, Facundo Jose Salinas, Hugo Hector Ortega, Fabrisio Alustiza, Maria Molina
Summary: This study reported the synthesis and performance of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM). The cytotoxicity, internalization ability, antigen protection and delivery capability of the nanogels were evaluated in vitro and in vivo. The results demonstrated that the pNIPAM nanogels can stimulate a humoral immune response.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Review
Polymer Science
Cesar A. Barbero, Diego F. Acevedo
Summary: This review discusses the mechanochemical synthesis of polyanilines (PANIs) through oxidative polymerization of anilines. The effects of different parameters on the properties of conducting polymers are analyzed, including molecular weight, degradation, doping/oxidation level, conductivity, and nanostructure. The production of acids during polymerization and the comparison of PANI nanostructures made by mechanochemical polymerization (MCP) and solution are also investigated. The extensive work on the production of nanocomposites by MCP of anilines together with different nanomaterials is described.
Article
Chemistry, Physical
Mariano M. M. Bruno, N. Gustavo Cotella, Cesar A. A. Barbero
Summary: By incorporating cellulosic fiber fabric into the resorcinol/formaldehyde (RF) precursor resins, the mechanical properties of porous hierarchical carbon were improved. The carbonized fiber fabric enhanced the elastic modulus of the composites. The adsorption of RF resin precursor onto the fabric stabilized its porosity during drying. The textural properties were evaluated, showing a surface area of 558 m^2/g. The electrochemical properties of the porous carbon were also evaluated, measuring specific capacitances of up to 182 Fg^-1 (CV) and 160 Fg^-1 (EIS).
Review
Polymer Science
Cesar A. Barbero
Summary: Hydrogels made of cPAM and conducting materials made of PANIs are widely used due to their accessible monomers, easy synthesis and excellent properties. The combination of these materials produces composites with enhanced properties and synergy between cPAM and PANIs. The composites can be formed by radical polymerization to create a gel and incorporating PANIs through oxidative polymerization. Technological applications of the composites include photothermal actuators, supercapacitors, and sensors.
Article
Chemistry, Physical
Maria Paula Militello, Maria Victoria Martinez, Luciano Tamborini, Diego F. Acevedo, Cesar A. Barbero
Summary: The synthesis and characterization of resin nanoparticles and carbonization and sulfonation processes are studied in this work. The properties and catalytic activity of the nanoparticles are investigated using acid/base titration and TGA methods. The results show that sulfonated nanoparticles can effectively catalyze ester exchange reactions and demonstrate photothermal catalysis phenomenon.
Review
Polymer Science
Silvestre Bongiovanni Abel, Evelina Frontera, Diego Acevedo, Cesar A. Barbero
Summary: This study summarizes the use of organic chemical reactions to functionalize preformed conducting polymers (CPs), focusing on polyaniline (PANI), polypyrrole (PPy), and polythiophene (PT), as well as limited work on other CPs. Two approaches have been taken for the functionalization: direct reactions on the CP chains and reaction with substituted CPs bearing reactive groups. Electrophilic aromatic substitution and nucleophilic conjugate addition are the main reaction mechanisms involved. The improved properties achieved by functionalization of different polymers are also discussed.
Article
Polymer Science
Ana Sofia Farioli, Maria Victoria Martinez, Cesar Barbero, Edith Yslas, Diego Acevedo
Summary: Biopolymers made by inverse vulcanization of soybean or sunflower oil with sulfur show potential for decontaminating water from hydrocarbons. The structure and remediation capability of these materials are influenced by the feedstock, and they can be reused multiple times.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Engineering, Environmental
Gustavo A. Monti, Gustavo A. Pino, Diego Acevedo
Summary: This study describes the synthesis and characterization of AuNCs supported on PNIPAM and PAAM polymer matrices. The nanomaterials are selective and sensitive for detecting mercury in water through fluorescence. The study also demonstrates the influence of polymer matrices on the selectivity of the nanomaterials.
ENVIRONMENTAL SCIENCE-ADVANCES
(2023)
Article
Food Science & Technology
Ramiro Torres-Gallo, Ricardo Duran, Jose Garcia-Camargo, Oswaldo Morales, Diofanor Acevedo, Diego F. Tirado
Summary: The study aimed to evaluate the impact of blending wheat flour with ackee aril flour on pasting and dough rheological properties. Gradual addition of ackee aril flour increased ash, fat, protein, and fiber content, while water and carbohydrate content showed opposite behavior.
INTERNATIONAL JOURNAL OF FOOD SCIENCE
(2021)
