Review
Chemistry, Analytical
Xiaoyu Zhou, Wenpeng Zhang, Zheng Ouyang
Summary: Mass spectrometry is gaining increasing attention in clinical applications, requiring technical innovations to transfer it from conventional laboratories to clinical settings. This review highlights recent advances in direct sampling ionization and miniature MS systems, discusses the future development trends, and the technical challenges raised by diseases.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Liang Chen, Alireza Ghiasvand, Estrella Sanz Rodriguez, Peter C. Innis, Brett Paull
Summary: Ambient ionization mass spectrometry (AIMS) has developed rapidly in recent years with the introduction of various new ionization techniques, while the application of nanomaterials has also received significant attention, effectively improving selectivity and ionization efficiency.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Margot De Spiegeleer, Vera Plekhova, Jozefien Geltmeyer, Ella Schoolaert, Beata Pomian, Varoon Singh, Kathleen Wijnant, Kimberly De Windt, Volter Paukku, Alexander De Loof, Inge Gies, Nathalie Michels, Stefaan De Henauw, Marilyn De Graeve, Karen De Clerck, Lynn Vanhaecke
Summary: In recent years, ambient ionization mass spectrometry (AIMS) has allowed for direct analysis of biofluid metabolome. However, AIMS procedures are hindered by analytical and practical limitations, which affect metabolome coverage. This study aimed to develop biofluid-specific metabolome sampling membranes (MetaSAMPs) to improve AIMS. Customized MetaSAMPs made of hydrophilic and lipophilic polymers demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis. This new approach was successfully validated and has potential applications in metabolic health stratification.
Review
Chemistry, Analytical
Chi-Wei Lee, Hung Su, Jentaie Shiea
Summary: Rapid identification of toxicants in cases of acute poisoning is crucial for emergency care, but many existing laboratory methods are not timely enough or too complex. Two ambient ionization mass spectrometric platforms, TD-ESI-MS and ELDI-MS, have been successfully used for qualitative and semi-quantitative analysis of toxicants and toxins in clinical specimens collected in emergency care scenarios. The goal is to enable nurses and emergency physicians to use these technologies without extensive pretreatment and understand the analytical reports they generate.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Vincen Wu, Jocelyn Tillner, Emrys Jones, James S. McKenzie, Dipa Gurung, Anna Mroz, Liam Poynter, Daniel Simon, Cristina Grau, Xavier Altafaj, Marc-Emmanuel Dumas, Ian Gilmore, Josephine Bunch, Zoltan Takats
Summary: This study examines the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue, comparing its performance to the traditional desorption electrospray ionization (DESI). The flow focusing mechanism in DESI offers advantages such as a rotationally symmetric electrospray jet, higher intensity, controllable parameters, and better portability. The optimized parameters for DEFFI result in similar images to DESI but with better sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Vera Plekhova, Kimberly De Windt, Margot De Spiegeleer, Marilyn De Graeve, Lynn Vanhaecke
Summary: Biofluid metabolomics is a valuable tool for discovering biomarkers related to disease, genetics, and exposure. Direct infusion and ambient ionization mass spectrometry offer attractive alternatives for rapid analysis with minimal sample handling.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hung Su, Zong-Han Jiang, Shu-Fen Chiou, Jentaie Shiea, Deng-Chyang Wu, Sung-Pin Tseng, Shu-Huei Jain, Chung-Yu Chang, Po-Liang Lu
Summary: An analytical platform combining probe sampling, thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS), and multivariate statistical analysis was developed to rapidly differentiate bacterial species based on the differences in their lipid profiles. The results were validated by protein fingerprinting using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF). The developed platform showed promising potential as a useful diagnostic tool for rapidly distinguishing bacterial species.
Article
Chemistry, Analytical
Xiaoping Zhang, Xiang Ren, Yuan Zhong, Konstantin Chingin, Huanwen Chen
Summary: The study detected ketones in exhaled breath using mass spectrometry and developed a fast, sensitive, noninvasive method for ketone analysis. Results showed higher levels of acetone in the breath of smokers compared to non-smokers.
Review
Chemistry, Analytical
Hong Zhang, Yali Yang, Yanxiao Jiang, Meng Zhang, Zhilong Xu, Xiaofei Wang, Jie Jiang
Summary: Mass spectrometry (MS) is an attractive analytical method in clinical analysis due to its high sensitivity, specificity and throughput. It has shown unique advantages over immunoassay and has become the golden criterion for many clinical applications. This review summarizes the characteristics and applications of MS, emphasizing its high efficiency in clinical research. Furthermore, it discusses the future prospects of MS technology, including the use of miniature MS instruments, point-of-care detection and high-throughput analysis for better development in clinical applications. Additionally, the potential applications of ambient ionization mass spectrometry (AIMS) in clinic are also summarized.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Katerina V. Djambazova, Jacqueline M. Van Ardenne, Jeffrey M. Spraggins
Summary: Imaging mass spectrometry allows for the untargeted mapping of biomolecules directly from tissue sections, with widespread clinical applicability in disease diagnosis and classification. This review summarizes the two most widely employed IMS technologies and their advancements in biomedical applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Nils Kunze-Szikszay, Maximilian Euler, Thorsten Perl
Summary: Traditional microbiological methods for diagnosing bacterial infections have led to long turnaround times, but detecting VOCs emitted by bacteria presents a potential innovative approach for rapid diagnosis. Spectrometric methods allow for the detection and quantification of bacterial VOCs with high sensitivity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Christopher C. Goudarzi, Ashley G. Woolfork, Arjun Sengupta, Aalim M. Weljie
Summary: Increased access to cheap and rapid mass spectrometry testing of biofluids is desired for the analysis of disorders and diseases related to metabolite or lipid level changes. This study establishes a customized high-throughput workflow for biological sample analysis using DESI-MS, which utilizes low-cost, open-source, and readily accessible materials. The workflow includes surface customization, high-throughput sample spotting, and an open-source interface for data analysis. The developed system allows for cost-effective custom surface design workflows and high-throughput analyses for a wide range of researchers.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Luan Felipe Campos Oliveira, David Ulisses Tega, Marcos Nogueira Eberlin, Alessandra Sussulini
Summary: V-EASI is a soft ambient ionization source suitable for samples in solution, without the need for voltage or heat, with low sample preparation requirements. The study validated the quantification method for bixin in annatto using the LC-V-EASI-MS system, showing good performance.
Review
Chemistry, Analytical
Jihye Kim, Jong-Yun Kim, Sang-Eun Bae, Kyuseok Song, Jong-Ho Park
Summary: The review focuses on quantifying I-129 using techniques such as TIMS, AMS, and ICP-MS, highlighting their advantages in sensitivity, analysis time, sample preparation, and applicability. Strategies discussed include reducing interference, matrix effects, improving detection limits, and the availability of standard materials.
MICROCHEMICAL JOURNAL
(2021)
Review
Spectroscopy
Hanlu Yue, Feiyao He, Zhongjun Zhao, Yixiang Duan
Summary: Ambient mass spectrometry (AMS) is a group of advanced analytical techniques that enable direct sampling and ionization of analytes in their native environment, offering advantages such as rapid and real-time analysis. Plasma-based AMS, as a significant category of AMS, has been widely applied in various fields and has undergone continuous development and innovation.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Biochemical Research Methods
Lisa Kobos, Christina R. Ferreira, Tiago J. P. Sobreira, Bartek Rajwa, Jonathan Shannahan
Summary: This study evaluated the impact of different storage conditions on signaling lipids, finding that time had the most significant effect on lipid sample changes, while temperature and solvent had relatively minor effects. The optimal storage condition for lipid extracts was found to be storing at -80 degrees Celsius without solvent for less than 2 weeks.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Madison E. Edwards, Thomas De Luca, Christina R. Ferreira, Kimberly S. Collins, Michael T. Eadon, Eric A. Benson, Tiago J. P. Sobreira, Robert Graham Cooks
Summary: A simple workflow for lipid profiling of EVs using microgram amounts of sample was reported. Human lymphocyte EVs and rat cell line EVs showed distinguishable lipid profiles in terms of protein and drug exposure as well as tissue origin.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Agriculture, Dairy & Animal Science
Thamiris Vieira Marsico, Jose Nelio de Sousa Sales, Christina Ramires Ferreira, Mateus Jose Sudano, Joao Henrique Moreira Viana, Luiz Sergio de Almeida Camargo, Marcos Nogueira Eberlin, Marcelo Marcondes Seneda, Pietro Sampaio Baruselli
Summary: This study aimed to characterize the lipid profiles of oocytes from different dairy cattle breeds (Bos indicus, Bos taurus, and their crossbreed), and revealed distinct lipid profile patterns between Gir and Holstein, while Gir and crossbreed oocytes showed greater similarities. The predominant lipids identified were phospholipids in Gir (Bos indicus) oocytes and triacylglycerol in Holstein (Bos taurus) oocytes.
Article
Biochemistry & Molecular Biology
Zhuoer Xie, Christina R. Ferreira, Alessandra A. Virequ, R. Graham Cooks
Summary: The use of MRM profiling in metabolomics and lipidomics can improve speed and efficiency by simplifying processes and reducing data volume, making sample classification and compound identification more effective.
CHEMISTRY AND PHYSICS OF LIPIDS
(2021)
Meeting Abstract
Agriculture, Dairy & Animal Science
Sarah Luecke, Aridany Suarez-Trujillo, Lea Logan, Kara R. Stewart, Christina Ferreira, Theresa Casey
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
A. Suarez-Trujillo, S. M. Luecke, L. Logan, C. Bradshaw, K. R. Stewart, R. C. Minor, C. Ramires Ferreira, T. M. Casey
Summary: This study investigated the changes in lipid composition of sow milk during lactation, finding differences in lipid classes and fatty acyl residue composition between different lactation days, while the relative concentration of certain lipid species remained constant. The abundance of phosphatidylglycerols and triacylglycerides increased with lactation progression, potentially reflecting the metabolic activity of the mammary gland and developmental needs of neonates.
Article
Agriculture, Dairy & Animal Science
Kayla M. Mills, Christina R. Ferreira, Jebadiah G. Stevens, Kara R. Stewart, Theresa M. Casey
Summary: By analyzing vaginal lipid profiles at 21 days postnatal, this study found that infertile replacement gilts had lower abundance of certain fatty acids and higher abundance of free fatty acids compared to high fertility gilts. Additionally, infertile gilts had higher levels of eicosapentaenoic acid, a precursor of prostaglandins. The potential of using a panel of lipids captured with vaginal swabs to predict the reproductive efficiency of replacement gilts shows promise for future research in this area.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
R. N. Klopp, C. R. Ferreira, T. M. Casey, J. P. Boerman
Summary: This study aimed to investigate the relationships between circulating and colostrum lipids in cows and calves, and the effects of maternal metabolic status on circulating and colostrum lipids. The results showed that circulating lipids in cows and calves were distinct from colostrum lipids, except for phosphatidylglycerol (PG), where colostrum appeared to serve as the source for PG in the calf's circulation.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Saeed Alqahtani, Li Xia, Amber Jannasch, Christina Ferreira, Jackeline Franco, Jonathan H. Shannahan
Summary: The study found that individuals with Metabolic Syndrome (MetS) experience exacerbated acute pulmonary inflammatory responses following exposure to silver nanoparticles (AgNP), with a more pronounced impact on inflammatory resolution signaling mediators compared to healthy mice. Treatment with 14-HDHA and 17-HDHA can alleviate the exacerbated inflammatory response in AgNP-exposed MetS mice, indicating a potential mechanism responsible for enhanced susceptibility in this population.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Cesar A. Marasco Junior, Madison E. Edwards, Rafaela S. Lamarca, Tiago J. P. Sobreira, Jeffrey M. Caterino, David S. Hains, Andrew L. Schwaderer, Paulo Clairmont F. de Lima Gomes, Christina R. Ferreira
Summary: Thousands of chemical compounds produced by industry are widely spread in the human environment and new chemicals continue to be introduced. This study proposed a screening workflow to detect exogenous compounds in human urine samples, allowing for the selection and further analysis of these compounds. The results of the screening suggested the presence of five exogenous compounds in the samples.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Review
Biochemical Research Methods
Gustavo de Sa Schiavo Matias, Rodrigo da Silva Nunes Barreto, Ana Claudia Oliveira Carreira, Milton Yutaka Nishiyama, Paula Fratini, Christina Ramires Ferreira, Maria Angelica Miglino
Summary: The placenta's ability for growth and remodeling is closely related to its extracellular matrix (ECM) components. A study on the proteome profile of decellularized canine placenta revealed proteins related to skeleton structuration, cell adhesion, vasculogenesis, and other functions. These findings support the importance of maintaining essential protein groups for ECM structuring and physiology in tissue engineering applications.
JOURNAL OF PROTEOMICS
(2022)
Article
Chemistry, Multidisciplinary
Li Xia, Saeed Alqahtani, Christina R. Ferreira, Uma K. Aryal, Katelyn Biggs, Jonathan H. Shannahan
Summary: Nanoparticles interact with biomolecules by forming a biocorona on their surface, which alters cell interactions and toxicity. Metabolic syndrome may result in distinct interactions between nanoparticles and biomolecules in the lungs, leading to the formation of unique biocoronas and enhanced toxicity.
Article
Reproductive Biology
Kelly Annes, Christina Ramires Ferreira, Roniele Santana Valente, Thamiris Vieira Marsico, Juliana Hayashi Tannura, Juliano Coelho da Silveira, Flavio Henrique Silva, Fernanda da Cruz Landim-Alvarenga, Fernando Silveira Mesquista, Mateus Jose Sudano
Summary: Each living organism is unique due to the specific lipid identity of its organelles, and the distribution of these molecules plays a role in cellular activity. Studying the lipid profiles of bovine embryos at the organelle level can provide valuable insights into key physiological processes during preimplantation development. This study aimed to characterize four organelles in vitro-produced bovine embryos and evaluate the lipid species associated with each organelle.
Article
Obstetrics & Gynecology
Thalita S. Berteli, Alessandra A. Vireque, Caroline M. Da Luz, Eduardo D. Borges, Christina R. Ferreira, Paula A. Navarro
Summary: This study found that longer exposure time to equilibration solutions during oocyte vitrification can affect embryonic development and induce changes in oocyte lipid composition. The addition of oleic and linoleic acids to the vitrification media can help preserve the integrity of oocyte membrane phospholipids.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Biochemistry & Molecular Biology
Nicolas M. Morato, Judy E. Hallett, Wen-Hung Wang, Bennett D. Elzey, Gregory M. Cresswell, Bruce R. Cooper, Christina R. Ferreira
Summary: The embryonic environment can modify cancer cell metabolism and properties, and this study investigates the changes in gene expression and lipid composition of tumor cells in response to co-culturing with mouse embryos or extracts. The findings suggest that small molecules secreted from and present within the embryos may be involved in inducing metabolic changes in the tumor cells.