Article
Chemistry, Analytical
Zi-Hong Xu, Jun-Kai Wang, Qiu-Xiong Ye, Lin-Feng Jiang, Hao Deng, Jun-Fa Liang, Run-Xi Chen, Wei Huang, Hong-Tao Lei, Zhen-Lin Xu, Lin Luo
Summary: A new hapten with a carboxyl-containing spacer arm was synthesized to elicit an anti-FPT monoclonal antibody. The monoclonal antibody exhibited high affinity and selectivity to FPT with minimal cross-reactivities. A fluorescence immunochromatographic assay (FICA) was developed based on the monoclonal antibody for the detection of FPT, which showed a much lower detection limit than the maximum residue limit for food samples. The FICA demonstrated good recovery rates and results consistent with gas chromatography-tandem mass spectrometry.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Riikka Peltomaa, Asmaa Abbas, Tapani Yli-Mattila, Urpo Lamminmaki
Summary: Due to the high carcinogenicity and economic implications of aflatoxins, developing a noncompetitive immunoassay for rapid and simple detection of aflatoxins is of great importance. In this study, a noncompetitive immunoassay based on a monoclonal capture antibody and a unique antiimmunocomplex (anti-IC) antibody fragment was developed, enabling the detection of aflatoxins with a low detection limit.
Review
Biochemistry & Molecular Biology
Ge Li, Qingmei Li, Xun Wang, Xiao Liu, Yuhang Zhang, Rui Li, Junqing Guo, Gaiping Zhang
Summary: Lateral flow immunoassay (LFIA) is a popular technique in point-of-care testing (POCT) for its accessibility, speed, and affordability, and has gained significant attention during the COVID-19 outbreak for its ability to provide immediate diagnosis to users. This review introduces the principles, key components, and various detection formats of LFIA for antigens, antibodies, and haptens, as well as the integration of new trends such as novel labels, multiplexing, and digital assays. The development and future perspectives of LFIA are also discussed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Analytical
Yuchen Bai, Jie Fei, Weilin Wu, Leina Dou, Minggang Liu, Shibei Shao, Wenbo Yu, Kai Wen, Jianzhong Shen, Zhanhui Wang
Summary: This study determined the minimum distance between two epitopes in sandwich immunoassays for small molecules. The results provide a theoretical basis for hapten design, antibody discovery and development, and the establishment of sandwich immunoassays.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Yuchen Bai, Yahui Wang, Qiang Li, Leina Dou, Minggang Liu, Shibei Shao, Jianyu Zhu, Jianzhong Shen, Zhanhui Wang, Kai Wen, Wenbo Yu
Summary: In this study, a novel hapten of halofuginone was synthesized to generate a monoclonal antibody, and a noncompetitive immunoassay was established to achieve higher sensitivity and wider analytical range compared to traditional competitive assays.
Article
Chemistry, Applied
Wenting Zou, Ruirui Shi, Guanqun Wang, Zhiling Zhao, Fengchun Zhao, Zhengyou Yang
Summary: In this study, a noncompetitive immunoassay for detecting AFB1 was developed using phage-display technology. Compared with competitive immunoassays, this method exhibited higher sensitivity and efficiency, and achieved satisfactory recovery rates in various sample matrices.
Article
Chemistry, Applied
He Chen, Tianyang You, Lingfeng Zong, Liliya Mukhametova, Dmitry O. Zherdev, Sergei A. Eremin, Yuan Ding, Minghua Wang, Xiude Hua
Summary: This study designed and synthesized different types of tracers for the development of competitive and noncompetitive FPIAs for benzothiostrobin. Tetramer tracers could generate a higher signal-to-noise ratio and sensitivity in the assays.
Article
Environmental Sciences
Lianrun Huang, He Chen, Panpan Cui, Yuan Ding, Minghua Wang, Xiude Hua
Summary: In this study, haptens with carboxylic groups were synthesized and used to produce antibodies, leading to the development of a rapid and accurate assay for determining PND. The assay showed low detection limit, minimal cross-reactivity with PND analogs, and good correlation with traditional analysis methods.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biophysics
Keine Nishiyama, Kazuki Takahashi, Mao Fukuyama, Motohiro Kasuya, Ayuko Imai, Takumi Usukura, Nako Maishi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Kyoko Hida, Koji Shigemura, Akihide Hibara, Manabu Tokeshi
Summary: This study presents an analytical method using fluorescence polarization immunoassay (FPIA) for rapid and quantitative detection of SARS-CoV-2 antibody in human serum. The FPIA system showed high accuracy in discriminating COVID-19 positive or negative serum samples. The total measurement time was about 20 minutes, and a serum volume of 0.25 μL was required, making it a rapid and convenient method for quantification of SARS-CoV-2 antibody.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Asanka Gurukandure, Subramaniam Somasundaram, Amanda S. N. Kurian, Niamat Khuda, Christopher J. Easley
Summary: This study reports a new nucleic acid nanostructure sensor that allows for the general detection of larger proteins by conjugating the protein's antibody-binding epitope to the DNA strand. The sensor was validated for the quantification of creatine kinase, a clinically relevant protein, through competitive immunoassay and signal-on mechanism. However, further improvement in the purification of DNA-epitope conjugates is needed.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Arantxa Valdivia, Fabian Tarin, Maria Jesus Alcaraz, Paula Pinero, Ignacio Torres, Francisco Marco, Eliseo Albert, David Navarro
Summary: This study compared three methods for detecting SARS-CoV-2-specific antibodies, and found that IgG/IgA flow cytometry-based immunoassay (FCI) had the highest number of positive results and showed the highest number of positive results for early collected sera (within 15 days). The positive percent agreement between FCI and other immunoassays was highest for Roche electrochemiluminiscent assay (ECLIA), while the negative percent agreement was higher between FCI and Diasorin chemiluminiscent assay (CLIA). Thus, FCI may have higher clinical sensitivity for serological diagnosis of SARS-CoV-2 infection compared to Roche ECLIA and Diasorin CLIA.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Adam S. Kinne, Erik J. Tillman, Sanofar J. Abdeen, Derrick E. Johnson, Elijah S. Parmer, Jacob P. Hurst, Brittany de Temple, Sherri Rinker, Timothy P. Rolph, Ronald R. Bowsher
Summary: Efruxifermin (EFX) is a fusion protein that shows potential for treating liver fibrosis due to NASH, a common metabolic disease without approved treatment. A sensitive immunoassay has been developed to measure the concentration of biologically active EFX in human serum, supporting pharmacokinetic assessments in NASH patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Virology
Jorge Camacho, Eliseo Albert, Joao Zulaica, Beatriz Alvarez-Rodriguez, Luciana Rusu, Beatriz Olea, Maria Jesus Alcaraz, Ron Geller, Estela Gimenez, David Navarro
Summary: The information provided by SARS-CoV-2 spike (S)-targeting immunoassays is important in clinical-decision making. The performance of two immunoassays, Elecsys (R) Anti-SARS-CoV-2 S assay and LIAISON (R) SARS-CoV-2 TrimericS IgG assay, was compared using sera from vaccinated and unvaccinated individuals. The results showed that the Roche assay had a higher number of positive results and overall sensitivity, and the concordance between the two assays varied depending on the vaccination and infection status.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Food Science & Technology
Luis G. Addante-Moya, Antonio Abad-Somovilla, Antonio Abad-Fuentes, Consuelo Agullo, Josep V. Mercader
Summary: Immunochemical methods for mycotoxin analysis require well-defined antigens and antibodies with outstanding binding properties. This study successfully synthesized antigens and prepared immunoagents with unequivocal structures, leading to the development of novel highly sensitive immunoassays selective of Alternaria mycotoxins. Through extensive evaluation, antibodies with high affinity were raised from generated immunoagents, establishing a structure-activity relationship between synthetic haptens and the specificity of the antibodies.
Article
Immunology
Lisha Ma, Wensheng Wang, Lisha Li, Ying Chen, Binxuan Chen, Miaoli Shao, Yongjun Cheng, Renfang Zhou
Summary: This study evaluated a novel fully automated immunoturbidimetric assay for the detection of anti-CCP antibodies and compared it with the conventional ELISA method. The results showed that the four anti-CCP assays provided similar diagnostic information for rheumatoid arthritis (RA), with Qiangsheng's method being potentially more useful for large scale clinical screening due to its shorter testing time.
FRONTIERS IN IMMUNOLOGY
(2022)