Review
Biochemistry & Molecular Biology
Juan P. Rodrigo, Mario Sanchez-Canteli, Fernando Lopez, Gregory T. Wolf, Juan C. Hernandez-Prera, Michelle D. Williams, Stefan M. Willems, Alessandro Franchi, Andres Coca-Pelaz, Alfio Ferlito
Summary: Tumor-infiltrating lymphocytes (TIL) have been shown to be prognostically significant in Laryngeal Squamous Cell Carcinoma (LSCC), with high stromal TIL, CD8+ TIL, and CD3+/CD4+ TIL associated with better clinical outcomes. Evaluation of TIL in HE sections and assessment of CD3+, CD4+, and/or CD8+ TIL levels may serve as predictive markers for patient prognosis in LSCC.
Article
Oncology
Joshua D. Smith, Emily L. Bellile, Susan E. Ellsperman, Molly E. Heft-Neal, Jacqueline E. Mann, Andrew C. Birkeland, Rebecca C. Hoesli, Paul L. Swiecicki, Francis P. Worden, Caitlin Schonewolf, Jennifer L. Shah, Michelle L. Mierzwa, Andrew J. Rosko, Chaz L. Stucken, Steven B. Chinn, Andrew G. Shuman, Keith A. Casper, Kelly M. Malloy, Mark E. P. Prince, Gregory T. Wolf, Dafydd G. Thomas, Jonathan B. McHugh, J. Chad Brenner, Matthew E. Spector
Summary: In patients with persistent or recurrent LSCC, CD103(+)TILs showed a modest correlation with PD-L1 CPS, while no other TIL subsets correlated with PD-L1 CPS. Patients with high CD103(+)TILs and/or high PD-L1 CPS staining exhibited the most favorable survival outcomes.
Article
Medicine, Research & Experimental
Duo Zhang, Di Tang, Yu Heng, Xiao-Ke Zhu, Liang Zhou, Lei Tao, Li-ming Lu
Summary: Immunohistochemical assessment of TILs and neutrophil densities in tumor tissues is associated with the prognosis of patients with LSCC, with high TILs density correlating with better prognosis, and high neutrophil density correlating with poorer prognosis.
Article
Pathology
Omar Kujan, Muhamed Agag, Monika Smaga, Yash Vaishnaw, Majdy Idrees, Kate Shearston, Camile S. Farah
Summary: This study aimed to evaluate the diagnostic utility of specific immunomodulator proteins and their role in the progression of oral squamous cell carcinoma (OSCC). The results showed that the PD-1/PD-L1 pathway, Treg-related proteins, and tumor infiltrating lymphocytes (TILs) were associated with the development of OSCC. Diagnostic models using these proteins and TILs accurately predicted the grade of oral epithelial disorder.
Article
Oncology
Menekse Goker, Stephanie Deblaere, Hannelore Denys, Glenn Vergauwen, Eline Naert, Liv Veldeman, Chris Monten, Rudy Van den Broecke, Jo Van Dorpe, Geert Braems, Koen Van de Vijver
Summary: This study investigated the expression of sTILs and PD-L1 in pleomorphic invasive lobular cancer (pILC). It found that sTILs were present in the majority of pILC patients, while PD-L1 expression was not associated with survival improvement.
Article
Immunology
Di Tang, Duo Zhang, Yu Heng, Xiao-Ke Zhu, Han-Qing Lin, Jian Zhou, Lei Tao, Li-Ming Lu
Summary: TANs play a significant role in the development and prognosis of LSCC by exhibiting low apoptosis and immunosuppressive phenotype. They impair anti-tumor immunity by suppressing proliferation and activation of T cells. Targeting PD-L1+TANs or GM-CSF as a new immunotherapeutic strategy may be beneficial for LSCC.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Immunology
Juan Wang, Limin Lun, Xin Jiang, Yi Wang, Xiaopeng Li, Guoqiang Du, Jin Wang
Summary: Laryngeal squamous cell carcinoma (LSCC) and hypopharyngeal squamous cell carcinoma (HSCC) significantly impact patients' quality of life, and immunotherapy plays a crucial role in cancer treatment. Studies have shown that the upregulation of PD-L1 and APE1 is associated with tumorigenesis and poor prognosis, while PD-1 and PD-L1 are overexpressed in LSCC and HSCC tissues.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Oncology
Romain Banchereau, Avantika S. Chitre, Alexis Scherl, Thomas D. Wu, Namrata S. Patil, Patricia de Almeida, Edward E. Kadel, Shravan Madireddi, Amelia Au-Yeung, Chikara Takahashi, Ying-Jiun Chen, Zora Modrusan, Jacqueline McBride, Rhea Nersesian, Ehab A. El-Gabry, Mark D. Robida, Jeffrey C. Hung, Marcin Kowanetz, Wei Zou, Mark McCleland, Patrick Caplazi, Shadi Toghi Eshgi, Hartmut Koeppen, Priti S. Hegde, Ira Mellman, W. Rodney Mathews, Thomas Powles, Sanjeev Mariathasan, Jane Grogan, William E. O'Gorman
Summary: CD8+ tissue-resident memory T (T-RM) cells expressing CD103 (ITGAE) are believed to actively suppress cancer progression, and their presence in tumors may predict response to immunotherapy. This study found that in inflamed tumors, CD103+ CD8+ T-RM cells exhibit a complex phenotype characterized by increased clonal expansion, expression of checkpoint regulators, and cytotoxic proteins, suggesting their involvement in antitumor response.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Oncology
Lata Singh, Mithalesh Kumar Singh, Maria Cristina Kenney, Martine J. Jager, Moshahid Alam Rizvi, Rachna Meel, Neiwete Lomi, Sameer Bakhshi, Seema Sen, Seema Kashyap
Summary: The study showed that the expression of PD-1 and PD-L1 in UM is related to TILs presence and patient prognosis. TILs promote the expression of PD-1 and PD-L1, impacting the DFS rate. Treatment of UM cells with IFN-gamma induced PD-1 expression, while also leading to increased PD-L1 expression.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Yufan Qiu, Yi Yang, Riyao Yang, Chunxiao Liu, Jung-Mao Hsu, Zhou Jiang, Linlin Sun, Yongkun Wei, Chia-Wei Li, Dihua Yu, Jin Zhang, Mien-Chie Hung
Summary: The study reveals that PD-1 can be secreted in an exosomal form by activated T cells and interact remotely with PD-L1. This interaction can attenuate the suppression of tumor-specific cytotoxic T cell activity by PD-L1, restoring tumor surveillance.
Article
Pathology
Nicoletta D'Alessandris, Innocenza Palaia, Angelina Pernazza, Federica Tomao, Anna Di Pinto, Lucia Musacchio, Martina Leopizzi, Valeria Di Maio, Irene Pecorella, Pierluigi Benedetti Panici, Carlo Della Rocca
Summary: Cervical cancer shows potential for immunotherapy, specifically in the neoadjuvant setting. The study found a significant association between PD-L1 expression on tumor cells and immune cells with TIL percentage, and a correlation between TILs and PD-L1 expression with pathological response to NACT. These results suggest that cervical cancer could be a promising target for immunotherapy, with PD-L1 expression potentially predicting response to neoadjuvant therapy.
Article
Health Care Sciences & Services
Bogdan Marian Caraban, Elena Matei, Georgeta Camelia Cozaru, Mariana Aschie, Mariana Deacu, Manuela Enciu, Gabriela Izabela Baltatescu, Anca Chisoi, Nicolae Dobrin, Lucian Petcu, Emma Gheorghe, Laurentiu-Tony Hangan, Mihai Catalin Rosu, Cristian Ionut Orasanu, Antonela-Anca Nicolau
Summary: This study aimed to characterize the PD-L1 expression in melanomas and its association with T cell infiltrates. The results showed that most of the PD-L1 positive tumors had moderate scores of CD4+ and CD8+ TILs in tumoral melanoma cells. PD-L1 expression was associated with different degrees of lymphocytic infiltration and with Breslow tumor thickness. PD-L1 expression is a predictive biomarker for discriminating the presence or absence of malign tumoral melanoma cells and an independent predictor of good prognosis in melanoma patients.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Oncology
Yunbi Ni, Julia Y. Tsang, Yan Shao, Ivan K. Poon, Fiona Tam, Ka-Ho Shea, Gary M. Tse
Summary: This study investigated the clinicopathological features of breast cancer associated with PD-L1 expression and its relationship with other immune components. The results showed a positive correlation between PD-L1 expression and higher tumor grade, morphological apocrine features, presence of necrosis, and higher stromal tumor-infiltrating lymphocytes (sTIL). PD-L1 expression was also associated with certain receptors and markers. Survival analysis revealed associations between PD-L1 expression and better disease-free survival (DFS) and breast cancer-specific survival (BCSS) in specific subtypes of breast cancer. The combination of PD-L1 expression and tumor-infiltrating lymphocytes (TIL) further refined the prognosis of breast cancer subtypes.
Article
Oncology
Fenglong Bie, He Tian, Nan Sun, Ruochuan Zang, Moyan Zhang, Peng Song, Lei Liu, Yue Peng, Guangyu Bai, Bolun Zhou, Shugeng Gao
Summary: The study found significant differences in PD-L1 expression, TILs, and TME characteristics between Caucasian and Asian LUAD patients. This suggests that specific immunotherapy strategies should be tailored separately for these two populations.
CLINICAL EPIGENETICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Songtao Zhang, Qiujie Yan, Song Wei, Xiaobo Feng, Miaomiao Xue, Lina Liu, Jili Cui, Yuanyuan Zhang
Summary: The study showed that the expression of CMTM6 and PD-1/PD-L1 was significantly higher in OSCC tissues compared to paracancerous tissues. PD-L1 expression was associated with clinical stage and lymph node metastasis in OSCC. qRT-PCR results confirmed the relationship between mRNA and protein expression.
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY
(2021)
Review
Cell Biology
Maria-Alexandra Papadimitriou, Konstantina Panoutsopoulou, Katerina-Marina Pilala, Andreas Scorilas, Margaritis Avgeris
Summary: Methylation of DNA/RNA and proteins is crucial for cellular homeostasis and development, and disrupted methylation imprinting is strongly associated with human pathologies, especially cancer. Recent evidence has shown that miRNAs play a significant role in regulating cellular methylation profiles during cancer onset and progression. Epi-miRNAs are a novel subclass of miRNAs that have potential clinical utility in cancer diagnostics and personalized therapeutics.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Medical Laboratory Technology
Konstantina Panoutsopoulou, Yueyang Liu, Margaritis Avgeris, Tobias Dreyer, Julia Dorn, Viktor Magdolen, Andreas Scorilas
Summary: The evaluation of miR-146a in triple-negative breast cancer (TNBC) could improve personalized prognosis and guide precision medicine decisions.
CLINICAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Maria Papasavva, Styliana Amvrosiou, Katerina-Marina Pilala, Konstantinos Soureas, Panayiota Christodoulou, Yuan Ji, Konstantinos Stravodimos, Damo Xu, Andreas Scorilas, Margaritis Avgeris, Maria-Ioanna Christodoulou
Summary: Cellular and molecular immune components, including interleukin-37 (IL-37) and its receptor SIGIRR, play a crucial role in the development and progression of bladder urothelial carcinoma (BLCA). IL-37 levels are associated with tumor development and longer overall survival in BLCA patients. Mutations in SIGIRR are related to increased infiltration of regulatory T cells and dendritic cells in the tumor. These findings suggest that IL-37 and SIGIRR could be potential prognostic and diagnostic markers for BLCA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Maria-Alexandra Papadimitriou, Konstantinos Soureas, Aristea-Maria Papanota, Panagiotis Tsiakanikas, Panagiotis G. G. Adamopoulos, Ioannis Ntanasis-Stathopoulos, Panagiotis Malandrakis, Maria Gavriatopoulou, Diamantis C. C. Sideris, Efstathios Kastritis, Margaritis Avgeris, Meletios-Athanasios Dimopoulos, Evangelos Terpos, Andreas Scorilas
Summary: The overexpression of miR-25 is associated with poor prognosis and disease progression in multiple myeloma patients, and it can be used as a powerful predictive tool for personalized treatment decisions.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Ioannis S. Pateras, Chloe Williams, Despoina D. Gianniou, Aggelos T. Margetis, Margaritis Avgeris, Pantelis Rousakis, Aigli-Ioanna Legaki, Peter Mirtschink, Wei Zhang, Konstantina Panoutsopoulou, Anastasios D. Delis, Stamatis N. Pagakis, Wei Tang, Stefan Ambs, Ulrika Warpman Berglund, Thomas Helleday, Anastasia Varvarigou, Antonios Chatzigeorgiou, Anders Nordstrom, Ourania E. Tsitsilonis, Ioannis P. Trougakos, Jonathan D. Gilthorpe, Teresa Frisan
Summary: The study found that short-term fasting enhances the sensitivity of triple negative breast cancer cells to chemotherapy and reduces associated toxic side effects. The research also revealed the mechanism by which fasting promotes cell death through increased oxidative stress and DNA damage. The safety and efficacy of combining short-term fasting with chemotherapy were further confirmed in a TNBC mouse model.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Obstetrics & Gynecology
Danai Mavreli, Mariana Theodora, George Lambrou, Margaritis Avgeris, Nikolas Papantoniou, Jan Treager-Synodinos, George Daskalakis, Aggeliki Kolialexi
Summary: This study aimed to identify differentially expressed proteins (DEPs) in first trimester maternal plasma between pregnant women with subsequent spontaneous moderate/late Preterm Delivery (sPTD) and women with term deliveries. A total of 236 DEPs associated with coagulation and complement cascade were identified in the sPTD group. Furthermore, the decreased levels of VCAM-1, SAA, and Talin-1 were confirmed using ELISA, suggesting their potential as predictive biomarkers for sPTD at 32-36 weeks of gestation.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Christos K. Kontos, Paraskevi Karousi, Pinelopi I. Artemaki, Ahmed Abdelgawad, Aspasia Dimitriadou, Nikolaos P. Machairas, Diamantis C. Sideris, Vasiliki Pappa, Andreas Scorilas, Mona Batish, Sotirios G. Papageorgiou
Summary: In this study, we identified several novel circRNAs produced by BAX and BCL2L12, characterized by diverse exon structures. Interestingly, visualization of the most abundant circRNAs showed distinct intracellular localization. Additionally, a complex expression pattern of BAX and BCL2L12 circRNAs was revealed in CLL patients and non-leukemic blood donors, suggesting a multifaceted role in B-cell CLL.
Article
Biochemistry & Molecular Biology
Christos K. Kontos, Despina Hadjichambi, Maria Papatsirou, Paraskevi Karousi, Spyridon Christodoulou, Diamantis C. Sideris, Andreas Scorilas
Summary: This study identifies 23 novel BOK circRNAs, which consist of both exonic and intronic regions of the BOK gene. These circRNAs could play a role in the molecular landscape and clinical investigation of ovarian and prostate cancer.
Article
Biochemistry & Molecular Biology
Elena Fountzilas, Maria Kouspou, Alexia Eliades, Kyriaki Papadopoulou, Evangelos Bournakis, Anna Goussia, Marinos Tsiatas, Achilleas Achilleos, Kyriakos Tsangaras, Gaetan Billioud, Charalambos Loizides, Christos Lemesios, Elena Kypri, Marios Ioannides, George Koumbaris, Sofia Levva, Ioannis Vakalopoulos, Athanasios Paliouras, Stavroula Pervana, Filippos Koinis, Redi Bumci, Athina Christopoulou, Soultana Meditskou, Amanda Psyrri, Ioannis Boukovinas, Anastasios Visvikis, Vasilios Karavasilis, George K. K. Koukoulis, Athanasios Kotsakis, Dimitrios Giannakis, George Fountzilas, Philippos C. C. Patsalis
Summary: The data on tumor molecular profiling of European patients with prostate cancer is limited. Our study aimed to assess the prevalence and significance of gene alterations in unselected patients with prostate cancer. We found that clinically relevant somatic gene alterations were present in a significant proportion of European patients with prostate cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Dareilena Karaviti, Marietta Charakida, Dimitra Dimopoulou, Irini Eleftheriou, Marianthi Liaska, Maria Papadaki, Despoina Maritsi, Irine-Ikbale Sakou, Antonios Marmarinos, Margaritis Avgeris, Dimitrios Gourgiotis, Nikos Spyridis, Maria Tsolia
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Glykeria N. Daneva, Andreas Scorilas
Summary: Recent advances in molecular biology have allowed for the identification of post-transcriptional marks on RNAs, leading to the emerging field of epitranscriptomics. Over 170 RNA modifications have been reported, playing crucial roles in RNA biogenesis, stability, and accuracy of transcription and translation. Dysfunctions in the enzymes responsible for RNA modifications can lead to human diseases and cancer. This review focuses on the landscape of epitranscriptomics in human RNAs, describing the most common modifications and their functional and regulatory roles, as well as their involvement in cellular homeostasis and disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Katerina Katsaraki, Christos K. Kontos, Gerasimos Ardavanis-Loukeris, Alexandros A. Tzovaras, Diamantis C. Sideris, Andreas Scorilas
Summary: This study investigates the regulatory effect of proteasome inhibitors (PIs) on miRNA expression in breast cancer (BrCa) cell lines. The results show a high heterogeneity in miRNA levels among different cell lines and different treatment durations. Three miRNAs (miR-1-3p, miR-421-3p, and miR-765-3p) are identified as potential key players in BrCa. Various biological processes, molecular functions, and pathways are predicted to be affected.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Genetics & Heredity
Paraskevi Karousi, Martina Samiotaki, Manousos Makridakis, Jerome Zoidakis, Diamantis C. Sideris, Andreas Scorilas, Thomas Carell, Christos K. Kontos
Summary: This study investigates the role of 3'-tRF-Cys(GCA) in gene expression regulation in HEK-293 cells and finds that it can regulate gene expression by affecting multiple cellular pathways. Additionally, the study identifies the direct interaction between 3'-tRF-Cys(GCA) and thymopoietin and its modulation of the protein's levels.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)