Article
Fisheries
Yun Ho Lee, Jung Yeol Park, Il Young Lee, Irfan Zidni, Han Kyu Lim
Summary: This study aimed to develop sperm cryopreservation techniques for stone flounder, and through experiments on key factors such as cryoprotective agents, diluents, equilibration times, and dilution ratios, optimal conditions for sperm cryopreservation were determined.
Article
Biochemistry & Molecular Biology
Pablo C. Caracciolo, Patricia Diaz-Rodriguez, Ines Ardao, David Moreira, Florencia Montini-Ballarin, Gustavo A. Abraham, Angel Concheiro, Carmen Alvarez-Lorenzo
Summary: The study developed bioresorbable small-diameter vascular grafts functionalized with heparin to promote endothelial cell growth and inhibit smooth muscle cells over-proliferation. The tensile properties of the heparin-functionalized vascular grafts resembled those of saphenous veins, indicating potential clinical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Pharmacology & Pharmacy
Siyu Liu, Rong Hu, Jie Du, Yuanjian Li, Xiaohui Li
Summary: The senescence and dysfunction of vascular endothelial cells are important features of diabetic vascular disease. High mobility group box-1 (HMGB1) may be involved in vascular injury in response to high glucose. Glycyrrhizin (GL) is an HMGB1 inhibitor that significantly reduces HMGB1 and protects blood vessels from damage.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Food Science & Technology
Ho-Jeong Bang, Mok-Ryeon Ahn
Summary: This study found that pinobanksin exhibits antiangiogenic effect by inducing endothelial cell apoptosis, and it can be developed as an antiangiogenic agent. The mechanism involves inactivation of the Raf/MEK/ERK pathway and activation of apoptotic signaling such as caspase cascades.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Cell Biology
Hiroki Takeuchi, Mikiko Nishioka, Tadashi Maezawa, Yuko Kitano, Kento Terada-Yoshikawa, Ryota Tachibana, Manabu Kato, Suong-hyu Hyon, Yuki Gen, Kayo Tanaka, Kuniaki Toriyabe, Masafumi Nii, Eiji Kondo, Tomoaki Ikeda
Summary: Due to the risks of infection and unstable supply associated with human serum albumin, there is a need to develop xeno-free human sperm cryopreservation reagents. Research findings suggest that the newly developed reagent containing glycerol, carboxylated poly-l-lysine, and raffinose is comparable to commercial reagents containing human serum albumin in terms of sperm motility, viability, and DNA fragmentation.
Article
Environmental Sciences
Jiajing Cui, Pingwei Wang, Shuqi Yan, Yujun Liang, Dongge Liu, Shuping Ren
Summary: The study aims to explore the role of ferroptosis in endothelial cell dysfunction and its underlying mechanisms.
Article
Biology
David Pruss, Huaqing Yang, Xing Luo, Dejia Liu, Jan Hegermann, Willem F. Wolkers, Harald Sieme, Harriette Oldenhof
Summary: Successful sperm vitrification requires finding the right balance between droplet size, sperm concentration, CPA type and concentration, and exposure time.
Article
Environmental Sciences
Sarah Easson, Radha Dutt Singh, Liam Connors, Taylor Scheidl, Larissa Baker, Anshul Jadli, Hai-Lei Zhu, Jennifer Thompson
Summary: This study found that the commonly used BPA substitute BPS can disturb redox balance and increase the production of reactive oxygen species (ROS), leading to impaired endothelial function.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Kejia Kan, Yifei Mu, Marielle Bouschbacher, Carsten Sticht, Natalia Kuch, Martin Sigl, Nuh Rahbari, Norbert Gretz, Prama Pallavi, Michael Keese
Summary: The study revealed a novel biphasic response of human umbilical vein endothelial cells to blue light illumination. Low fluence blue light promoted cell activities, while high fluence illumination had the opposite effect by upregulating pro-apoptotic signaling pathways. These findings provide insights into photobiomodulation by blue light and potential treatment strategies for angiogenesis-dependent diseases.
Article
Chemistry, Medicinal
Chin-Feng Hsuan, Thung-Lip Lee, Wei-Kung Tseng, Chau-Chung Wu, Chi-Chang Chang, Tsui-Ling Ko, Ya-Ling Chen, Jer-Yiing Houng
Summary: The study demonstrated that extracts from Glossogyne tenuifolia (GT) can significantly increase nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs), with the root methanol extract showing the most potent effect. The root methanol extract increased NO production by increasing eNOS expression and activating eNOS phosphorylation at serine(1177).
Article
Biochemistry & Molecular Biology
Zeqing Zhao, Yaxi Sun, Qingchen Qiao, Li Zhang, Xianju Xie, Michael D. Weir, Abraham Schneider, Hockin H. K. Xu, Ning Zhang, Ke Zhang, Yuxing Bai
Summary: The study successfully prevascularized the CPC scaffold by co-culturing hPDLSCs and hUVECs, showing excellent osteogenic and angiogenic capabilities. The co-culture group outperformed monoculture group, presenting promising applications in dental, craniofacial, and orthopedic fields.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Chikahiro Imashiro, Tetsuya Azuma, Shun Itai, Taiki Kuribara, Kiichiro Totani, Hiroaki Onoe, Kenjiro Takemura
Summary: In this study, it was found that ultrasound can promote vasculogenesis in vitro. The use of regulated ultrasound on three-dimensional-monocultured HUVECs clarified the effect of ultrasound on vasculogenesis, showing enhanced protein expression related to vasculogenesis and response to mechanical stress. This finding may be an innovation in the tissue engineering field.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Pharmacology & Pharmacy
Wei Liu, Yanghui Ou, Yumeng Yang, Xuemei Zhang, Liqi Huang, Xiaohua Wang, Buling Wu, Mingcheng Huang
Summary: The study demonstrated that punicalagin has a protective effect on HUVECs activation through an endothelial-mediated mechanism, which may be used in the treatment of various disorders such as cancer, rheumatoid arthritis, and cardiovascular disease. This effect is achieved by reducing adhesion of monocyte cells to HUVECs, decreasing expression of ICAM-1 and VCAM-1, and inhibiting permeability, proliferation, migration, and tube formation in VEGF-induced HUVECs. Additionally, punicalagin suppresses IKK-mediated activation of NF-kappa B signaling and inhibits VEGFR2 activation and downstream p-PAK1.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lukasz Gwozdzinski, Joanna Bernasinska-Slomczewska, Pawel Hikisz, Anna Wiktorowska-Owczarek, Edward Kowalczyk, Anna Pieniazek
Summary: In this study, the properties of human varicose vein endothelial cells (HVVEC) and human umbilical vein endothelial cells (HUVEC) were compared. Three bioactive compounds, diosmin, escin, and bromelain, were tested on the cells. The drugs showed different effects on reactive oxygen species and nitric oxide generation in the cells. The study revealed a potential mechanism of action for VV therapy drugs on HVVEC cells.
Article
Genetics & Heredity
Regina L. Binz, Rupak Pathak
Summary: This study investigated the cytogenetic integrity of primary human umbilical vein endothelial cells (HUVECs) and found that approximately 50% of the cells have a deletion of the Xq terminal region. Additionally, a similar Xq deletion was identified in the daughter cell line generated by fusing HUVECs with A549 cells.
Article
Anatomy & Morphology
Jenna Dumbleton, James G. Shamul, Bin Jiang, Pranay Agarwal, Haishui Huang, Xiaofeng Jia, Xiaoming He
Summary: This study investigates directed neural differentiation of murine ESCs using a non-planar microfluidic device to encapsulate cells in 3D microcapsules with alginate hydrogel shells. Modifications of the hydrogel shell facilitate early neurite extension and demonstrate potential for further neural differentiation into specialized neurons for therapeutic applications.
CELLS TISSUES ORGANS
(2022)
Article
Engineering, Biomedical
Bin Jiang, Wenquan Ou, James G. Shamul, Hao Chen, Sarah Van Belleghem, Samantha Stewart, Zhenguo Liu, John P. Fisher, Xiaoming He
Summary: The use of Rock inhibitor (RI) in 3D cardiac differentiation of human induced pluripotent stem cells (iPSCs) poses challenges, but reducing the RI concentration can improve efficiency and homogeneity, which is important for understanding and treating heart diseases.
BIOACTIVE MATERIALS
(2022)
Article
Thermodynamics
Elyahb Allie Kwizera, Samantha Stewart, Md Musavvir Mahmud, Xiaoming He
Summary: Magnetic nanoparticles, especially superparamagnetic iron oxide nanoparticles, have garnered significant attention for their easy synthesis, functionalization, and control over size and shape. They have shown potential in various biomedical applications, including thermal treatments for cancer and enhancement of cryopreservation. Challenges in the use of magnetic nanoparticles in these biomedical applications are also discussed.
JOURNAL OF HEAT TRANSFER-TRANSACTIONS OF THE ASME
(2022)
Article
Polymer Science
Chen Gao, Junbo Jiang, Juan Wang, Yutong Wang, Yangyang Zhang, Caihua Rao, Yue Cheng, Zhongrong Chen, Runhuai Yang, Gang Zhao
Summary: The mineralization of hydroxyapatite mineralized by acidic metal oxides in orthopedic substitutes has been extensively researched. In this study, Polycaprolactone (PCL) nanofibers with nano-tantalum oxide (Ta2O5) were successfully fabricated through electrospinning. The chemical reaction between hydrophobic scaffolds and acid oxides in the mineralization process has a significant impact on the biological effects of the scaffolds.
JOURNAL OF APPLIED POLYMER SCIENCE
(2022)
Article
Materials Science, Biomaterials
Jianbo Yao, Lingxiao Shen, Zhongrong Chen, Bing Zhang, Gang Zhao
Summary: This study explored a microencapsulation method for cryopreservation of red blood cells (RBCs). The results showed that the recovery of RBCs after microencapsulation was significantly improved, and the washing process was simplified. The microencapsulation method provides a promising, referenceable, and more practical strategy for future clinical transfusion medicine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Materials Science, Multidisciplinary
Chao Ma, Meng Wang, Pierre Claver Uzabakiriho, Gang Zhao
Summary: A high-performance, safe, comfortable, waterproof, breathable, economical, and reliable wearable strain sensor was developed through mature electrospinning and screen-printing technology. The sensor exhibits excellent repeatability and stability, remarkable sensitivity, a broad working strain range, and an ultrafast response time. Its mechanical properties are similar to natural skin and it has unmatched biofriendliness.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Optics
Mengduo Jiang, Meng Shao, Xiao Yang, Linna He, Tao Peng, Tao Wang, Zeyu Ke, Zixin Wang, Shu Fang, Yuxin Mao, Xilin Ouyang, Gang Zhao, Jinhua Zhou
Summary: Classification of the morphology of red blood cells (RBCs) is crucial for evaluating the quality of long-term stored blood. This study combines quantitative phase imaging (QPI) technology with deep learning to automatically classify RBC morphology. The developed method shows higher accuracy and shorter processing time compared to traditional methods, especially for RBCs with more discocytes.
INTERNATIONAL JOURNAL OF OPTICS
(2022)
Article
Nanoscience & Nanotechnology
Meng Wang, Kai Wang, Chao Ma, Pierre Claver Uzabakiriho, Xi Chen, Gang Zhao
Summary: In this study, a mechanical gradient strategy was developed to fabricate high-performance stretchable electronic devices. By using polyvinyl alcohol glue to fix integrated circuits on stretchable circuits, the strain gradient during the stretching process was achieved, ensuring the stability and reliability of the devices. The flexible electronic devices fabricated by this method are highly stretchable and comfortable, making them suitable for various applications.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Genetics & Heredity
Pei-Chih Lee, Samantha Stewart, Olga Amelkina, Hannah Sylvester, Xiaoming He, Pierre Comizzoli
Summary: Intracellular trehalose delivery through CRNPs improves the dehydration tolerance of COCs, opening up new possibilities for oocyte storage and preservation of fertility at ambient temperatures.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Retraction
Chemistry, Multidisciplinary
Hai Wang, Pranay Agarwal, Gang Zhao, Guang Ji, Christopher M. Jewell, John P. Fisher, Xiongbin Lu, Xiaoming He
ACS CENTRAL SCIENCE
(2023)
Article
Engineering, Biomedical
Samantha Stewart, Wenquan Ou, Helim Aranda-Espinoza, Shaik O. Rahaman, Xiaoming He
Summary: Ovarian follicles develop in a regulated mechanical microenvironment, and disruptions to this microenvironment can cause infertility. The viscoelastic properties of ovarian tissue have not been extensively studied. This study characterizes the elastic and viscoelastic properties of ovarian tissue from both reproductive age groups of domestic cats and identifies possible connections between mechanical and compositional heterogeneities. These findings may have important implications for biomimetic follicle culture.
ACTA BIOMATERIALIA
(2023)
Article
Biotechnology & Applied Microbiology
Liyuan Zhang, Mengqiao Chi, Yue Cheng, Zhongrong Chen, Yunxia Cao, Gang Zhao
Summary: This study proposes a new method to improve the antioxidant capacity of whole ovaries during cryopreservation, static magnetic field assisted thawing. The results show that the antioxidant capacity of ovarian tissue was significantly improved by static magnetic field treatment. In addition, successful vascular regeneration and follicular development were achieved through ovarian tissue allograft transplantation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Cell & Tissue Engineering
Xiaohu Wang, Enyu Wang, Gang Zhao
Summary: This review provides an overview of the background and basic principles of cryopreservation in the field of stem cells. It discusses the main bioengineering technologies and strategies used in cryopreservation, as well as the current challenges and future prospects.
Review
Reproductive Biology
Pierre Comizzoli, Xiaoming He, Pei-Chih Lee
Summary: Preserving germ cells and gonadal tissues for the long term is critical in fertility treatment and animal population management. Current options at freezing temperatures are often constraining, but recent research suggests alternative strategies based on dehydration and storage at supra-zero temperatures. Further studies are needed for rehydration and reanimation of germ cells.
REPRODUCTION AND FERTILITY
(2022)