Article
Chemistry, Medicinal
Jussara J. Simao, Maysa M. Cruz, Fernanda M. Abdala, Andressa Bolsoni-Lopes, Lucia Armelin-Correa, Maria Isabel C. Alonso-Vale
Summary: The study demonstrates that palmitoleic acid (n7) can alleviate or prevent low-grade chronic inflammation caused by obesity by inducing adipocyte differentiation and reducing the expression of inflammatory cytokines.
Article
Cell & Tissue Engineering
Fermin Robledo, Lila Gonzalez-Hodar, Pablo Tapia, Ana-Maria Figueroa, Fernando Ezquer, Victor Cortes
Summary: Researchers successfully extracted multipotent cells from the brown adipose tissue of newborn mice, forming adipose spheroids with complex organoid structure and enhanced leptin secretion upon insulin stimulation. These findings have important implications for the potential therapeutic application of adipose tissue-derived stromal vascular fraction cells.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Hsiao-Chi Lai, Pei-Hsuan Chen, Chia-Hua Tang, Lee-Wei Chen
Summary: DPP4 stimulation may be a novel therapy to increase local adipogenesis and systemic adiponectin levels, as it increases the expression of adipogenesis-related genes and the population of DPP4(+) cells among PDGFR alpha(+) cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Anna Kornmuller, Tyler T. Cooper, Ammi Jani, Gilles A. Lajoie, Lauren E. Flynn
Summary: This study investigated the effects of expanding human adipose-derived stromal cells (hASCs) on microcarriers fabricated from decellularized adipose tissue (DAT) or decellularized cartilage tissue (DCT). The results showed that hASCs cultured on DCT microcarriers had a significantly higher cell density and altered gene and protein expression related to cell adhesion and ECM remodeling. The DCT microcarriers also promoted the secretion of IL-8 and PDGFAA by hASCs. Furthermore, microcarrier-cultured hASCs showed enhanced adipogenic differentiation.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Biology
Laura Le Pelletier, Matthieu Mantecon, Jennifer Gorwood, Martine Auclair, Roberta Foresti, Roberto Motterlini, Mireille Laforge, Michael Atlan, Bruno Feve, Jacqueline Capeau, Claire Lagathu, Veronique Bereziat
Summary: As individuals age, adipose-derived stromal cells (ASCs) exhibit distinct characteristics, with in vitro aging experiments showing the presence of senescence and oxidative stress.
Article
Polymer Science
Hwajung Lee, Hye Jin Hong, Sujeong Ahn, Dohyun Kim, Shin Hyuk Kang, Kanghee Cho, Won-Gun Koh
Summary: In this study, a one-pot double-crosslinked hydrogel was synthesized using bio-orthogonal chemistry for efficient encapsulation of stem cells and improved cell function. The addition of degradable components within the hydrogel regulated matrix stiffness and enhanced adipogenic differentiation efficiency. The synthetic (PEG) and natural (collagen) hybrid network showed adequate mechanical strength and higher cell adhesiveness, demonstrating better proliferation and function of stem cells compared to single-crosslinked hydrogels.
Article
Integrative & Complementary Medicine
Jung Hwan Oh, Fatih Karadeniz, Youngwan Seo, Chang-Suk Kong
Summary: This study found that treatment with hyunganol II (HNG) can inhibit adipogenesis of human bone marrow mesenchymal stromal cells (hBM-MSCs) and is associated with alterations in the MAPK signaling pathway. The findings suggest that HNG may prevent bone marrow adiposity by suppressing hBM-MSC adipogenesis and has potential as a drug or nutraceutical.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Cell & Tissue Engineering
Daisy D. Canepa, Elisa A. Casanova, Eirini Arvaniti, Vinko Tosevski, Sonja Maersmann, Benjamin Eggerschwiler, Sascha Halvachizadeh, Johanna Buschmann, Andre A. Barth, Jan A. Plock, Manfred Claassen, Hans-Christoph Pape, Paolo Cinelli
Summary: This study utilized CyTOF technology to explore the cellular composition of human AD-MSCs at single-cell level, revealing high heterogeneity and variability in the subcellular composition of AD-MSCs, with the identification of an ALP+/CD73+ subpopulation displaying enhanced osteogenic differentiation potential.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Nicolo Costantino Brembilla, Ali Modarressi, Dominik Andre-Levigne, Estelle Brioudes, Florian Lanza, Hubert Vuagnat, Stephane Durual, Laurine Marger, Wolf-Henning Boehncke, Karl-Heinz Krause, Olivier Preynat-Seauve
Summary: This study evaluates the influence of a gelatin sponge on adipose-derived stromal cells (ASC). The results show that the gelatin sponge strongly influences the transcriptome of ASC and acted as a concentrator and reservoir of the regenerative ASC secretome. The gelatin sponge also increased angiogenesis in vivo and promoted wound healing.
Article
Cell Biology
Isis Cortes, Gutemberg Alves, Cesar Claudio-Da-Silva, Leandra Santos Baptista
Summary: This study found that different subpopulations of adipose stem/stromal cells (ASCs) exhibit distinct physiological properties in a 3D spheroid culture. ASC spheroids from superficial subcutaneous adipose tissue (sSAT) showed the highest response to adipogenic induction, while spheroids from deep subcutaneous adipose tissue (dSAT) showed the greatest lipolytic capacity. Spheroids from superficial retinacula cutis (sRC) exhibited a greater secretory capacity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Chemistry, Medicinal
Milena Deptula, Agnieszka Brzezicka, Aneta Skoniecka, Jacek Zielinski, Michal Pikula
Summary: Wound healing complications are a significant burden economically and medically, especially for patients with comorbidities such as diabetes and cancer. Current treatment methods are not fully effective, leading to a search for new solutions, with cell-based therapies showing potential. AD-MSCs, easily obtained from adipose tissue, have high proregenerative potential mainly through paracrine activities.
MEDICINAL RESEARCH REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Giovannamaria Petrocelli, Provvidenza Maria Abruzzo, Luca Pampanella, Riccardo Tassinari, Serena Marini, Elena Zamagni, Carlo Ventura, Federica Facchin, Silvia Canaider
Summary: This study investigated the effects of oxytocin on the proliferation, migratory ability, senescence, and autophagy of human adipose-derived stem cells (hASCs). The results showed that oxytocin did not affect hASC proliferation and migratory ability, but promoted autophagy and osteogenic differentiation. This suggests that oxytocin may promote osteogenesis in hASCs by modulating the autophagic process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
In Sil Park, Youngjin Han, HyunA Jo, Ki Won Lee, Yong Sang Song
Summary: Resveratrol and piceatannol, major stilbene compounds found in natural foods, exhibit inhibitory effects on lipid accumulation and adipogenesis, with piceatannol showing superior anti-adipogenic properties compared to resveratrol in a model of visceral obesity.
Article
Nutrition & Dietetics
Antonio Casado-Diaz, Angel Rodriguez-Ramos, Barbara Torrecillas-Baena, Gabriel Dorado, Jose Manuel Quesada-Gomez, Maria Angeles Galvez-Moreno
Summary: Study found that phloretin can inhibit human adipogenesis by increasing beta-catenin and inducing apoptosis in adipocytes during late differentiation. This suggests the potential of phloretin-rich foods for clinical use and regenerative-medicine applications to combat obesity and prevent bone-marrow adiposity.
Article
Oncology
Ronnie L. Shammas, Geoffroy C. Sisk, Christopher J. Coroneos, Anaeze C. Offodile Ii, Rene D. Largo, Arash Momeni, Nicholas L. Berlin, Summer E. Hanson, Adeyiza O. Momoh, Jonas A. Nelson, Evan Matros, Kristen Rezak, Brett T. Phillips
Summary: This study utilized a Delphi process to define a textbook outcome in DIEP flap breast reconstruction and identified exclusion criteria for future studies. Most surgeons believed that textbook outcomes are effective metrics for evaluating the quality of breast reconstruction care.
BREAST CANCER RESEARCH AND TREATMENT
(2023)
Article
Surgery
Rachel Pedreira, Ruth Tevlin, Michelle Griffin, Derrick Wan, Arash Momeni
Summary: This study investigates the impact of reconstructive modality on complication severity in breast reconstruction. The results show a significant association between implant-based breast reconstruction and major complications, and autologous breast reconstruction and minor complications.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2023)
Article
Multidisciplinary Sciences
Ruth Tevlin, Michelle Griffin, Kellen Chen, Michael Januszyk, Nick Guardino, Amanda Spielman, Shannon Walters, Garry Evan Gold, Charles K. F. Chan, Geoffrey C. Gurtner, Derrick C. Wan, Michael T. Longaker
Summary: Mandibular distraction osteogenesis (DO) is mediated by skeletal stem cells (SSCs) in mice, which promote bone regeneration through neural crest re-activation. Denervation prior to DO leads to reduced SSC amplification and osteogenic potential. Innervated SSCs dominate clusters related to bone formation.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Norah E. Liang, Michelle F. Griffin, Charlotte E. Berry, Jennifer B. Parker, Mauricio A. Downer, Derrick C. Wan, Michael T. Longaker
Summary: Surgical implants have limitations due to the body's immune response, known as the foreign body response (FBR). Acellular dermal matrix (ADM) has shown promise in reducing the fibrotic reaction caused by FBR, but its mechanism is still unclear. This article provides an overview of ADM use in various surgical models and anatomical applications.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Medicine, General & Internal
Jennifer B. Parker, Michelle F. Griffin, Mauricio A. Downer, Deena Akras, Charlotte E. Berry, Asha C. Cotterell, Geoffrey C. Gurtner, Michael T. Longaker, Derrick C. Wan
Summary: Translating basic science research into clinical practice has benefits, but most therapies fail to gain approval. The gap between basic research and approved treatment continues to grow, with long approval processes. Recent research on DFO shows promise for treating radiation-induced soft tissue injury.
FRONTIERS IN MEDICINE
(2023)
Review
Surgery
Charlotte E. E. Berry, Mauricio Downer, Annah G. G. Morgan, Michelle Griffin, Norah E. E. Liang, Lionel Kameni, Jennifer B. B. Laufey Parker, Jason Guo, Michael T. T. Longaker, Derrick C. C. Wan
Summary: Wound healing can lead to scar tissue formation, which has negative impacts on function, mental health, and the economy. Excessive fibroblast activity and accumulation of extracellular matrix proteins contribute to pathological scar formation. Studies have begun to uncover the cellular mechanisms that underlie the increased scar formation due to mechanical stress on wounds.
FRONTIERS IN SURGERY
(2023)
Editorial Material
Surgery
Charlotte E. Berry, Michael T. Longaker, Derrick C. Wan
AESTHETIC SURGERY JOURNAL
(2023)
Article
Surgery
Charlotte E. Berry, Alexander Z. Fazilat, Andrew A. Churukian, Darren B. Abbas, Michelle Griffin, Mauricio Downer, Micheal Januszyk, Arash Momeni, Shane D. Morrison, Derrick C. Wan
Summary: This study examines the readability, accessibility, and quality of online resources related to gender-affirming surgery (GAS) using search engine trend analysis, readability formulas, and DISCERN score. The results show an increasing frequency of GAS-related searches, but most online resources have a readability level above patients' proficiency, making them difficult to understand. Although patient-facing online information about GAS is somewhat accessible, it has low quality. There is a need to improve the quality and readability of online GAS resources to meet the needs of patients.
PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN
(2023)
Meeting Abstract
Surgery
Charlotte Berry, Michelle Griffin, Lionel Kameni, Darren Abbas, Mauricio Downer, Jennifer B. Parker, Norah Liang, Michael Januszyk, Michael T. Longaker, Derrick C. Wan
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2023)
Meeting Abstract
Surgery
Charlotte Berry, Lionel Kameni, Michelle Griffin, Mauricio Downer, Jennifer B. Parker, Jason L. Guo, Norah Liang, Michael Januszyk, Michael T. Longaker, Derrick C. Wan
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2023)
Meeting Abstract
Surgery
Charlotte Berry, Darren Abbas, Lionel Kameni, Michelle Griffin, Mauricio Downer, Jennifer B. Parker, Norah Liang, Jason L. Guo, Michael T. Longaker, Derrick C. Wan
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2023)
Meeting Abstract
Surgery
Charlotte Berry, Darren Abbas, Michelle Griffin, Lionel Kameni, Mauricio Downer, Jennifer B. Parker, Norah Liang, Sarah Dilorio, Michael T. Longaker, Derrick C. Wan
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2023)
Meeting Abstract
Surgery
Charlotte Berry, Darren Abbas, Lionel Kameni, Michelle Griffin, Mauricio Downer, Jennifer B. Parker, Jason L. Guo, Michael Januszyk, Michael T. Longaker, Derrick C. Wan
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2023)
Meeting Abstract
Surgery
Sarah Diiorio, Jason L. Guo, Michelle Griffin, Julika L. Huber, Mauricio Downer, Charlotte Berry, Derrick C. Wan, Michael T. Longaker
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2023)