Article
Multidisciplinary Sciences
Min Wang, Lili Li, Jun Qian, Nannan Wang, Jun Bao, Jiangyue Lu, Faming Chen, Yanfen Li, Yangheng Zhang, Fuhua Yan
Summary: In this study, the salivary microbiota from patients with periodontitis was transferred to obese mice induced by high-fat diet to investigate the influence on gut microbiome and liver metabolism. The results showed that periodontitis salivary microbiota altered gut microbiota composition and exacerbated intestinal barrier dysfunction, leading to activation of toll-like receptor 4 signaling and release of pro-inflammatory factors in the liver. Moreover, the upregulation of the tryptophan-kynurenine-AhR signal axis in the liver was associated with aggravated hepatic steatosis and dysregulation of glucolipid metabolism during NAFLD development. This study suggests that periodontitis salivary microbiota may worsen the pathological progression of NAFLD in the context of obesity, with the tryptophan-AhR pathway potentially playing a key role.
Article
Multidisciplinary Sciences
Anastasios Karydis, Indra Sandal, Jiwen Luo, Amanda Prislovsky, Amanda Gamboa, Edward F. Rosloniec, David D. Brand
Summary: The study found that carrying the shared epitope and inoculation with Porphyromonas gingivalis increased the percentage of Th17 cells, significantly increased bone loss, and allowed for the generation of CCP2 ACPAs not found in C57BL/6 or DBA/1 arthritic mouse serum.
Article
Biochemistry & Molecular Biology
Teppei Okabe, Yosuke Kamiya, Takeshi Kikuchi, Hisashi Goto, Masayuki Umemura, Yuki Suzuki, Yoshihiko Sugita, Yoshikazu Naiki, Yoshiaki Hasegawa, Jun-ichiro Hayashi, Shotaro Kawamura, Noritaka Sawada, Yuhei Takayanagi, Takeki Fujimura, Naoya Higuchi, Akio Mitani
Summary: This study found that the culture supernatant of Porphyromonas gingivalis accelerated inflammation in the lungs of Streptococcus pneumoniae-infected mice, and increased inflammatory cell infiltration. Compared to S. pneumoniae infection alone, PgSup synergistically increased TNF-alpha and IL-17 expression/production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Banndith Cheat, Coralie Torrens, Asmaa Foda, Brigitte Baroukh, Jeremy Sadoine, Lotfi Slimani, Veronique Witko-Sarsat, Olivier Huck, Marjolaine Gosset, Jerome Bouchet
Summary: Our study investigates the role of NLRP3 in periodontitis, demonstrating its ambiguous role in neutrophils and its interaction with Porphyromonas gingivalis. The study reveals that NLRP3 is involved in regulatory pathways that limit periodontitis in mouse models, but its protective effect is diminished in the presence of P. gingivalis. Overexpression of NLRP3 in connective tissue leads to increased production of mature IL-1 beta and mobilization of neutrophils, while NLRP3 deficiency results in lower IL-1 beta expression and almost no neutrophil mobilization.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ahmed Hamarsha, Kumarendran Balachandran, Ahmad Tarmidi Sailan, Nurrul Shaqinah Nasruddin
Summary: The aim of this study was to investigate the relationship between P. gingivalis and AD, and explore the role of genes and molecular targets. Through gene expression analysis, 10 common genes associated with both diseases were identified, and molecular simulations were conducted. The findings of this study revealed novel genes related to P. gingivalis-associated AD that require further validation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Aili Zhang, Zuokun Lu, Yanhao Xu, Tiantian Qi, Wenwen Li, Liang Zhang, Zhaohui Cui
Summary: The enzymes PPX/GppA play crucial roles in the bacterial stringent response by degrading inorganic polyphosphate to support cell survival. The crystal structure analysis of PPX from Porphyromonas gingivalis provides insights into how this enzyme functions and interacts with magnesium ions and substrate analogs.
JOURNAL OF STRUCTURAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Gina R. Lewin, Kendall S. Stocke, Richard J. Lamont, Marvin Whiteley
Summary: This study characterizes the behavior and virulence of the periodontal pathogen Porphyromonas gingivalis during human infection. It reveals that P. gingivalis is highly expressed in diseased samples, with genes related to virulence factors and growth/matabolism being actively expressed. The study also shows that the gene expression of P. gingivalis during periodontitis is highly conserved with logarithmic laboratory growth, suggesting that simple test tube growth can be used as a model for studying P. gingivalis biology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Analytical
Seonhwa Park, Kiryeon Park, Hee Sam Na, Jin Chung, Haesik Yang
Summary: An electrochemical biosensor for sensitive detection of Porphyromonas gingivalis in saliva was developed without the need for washing and separation steps. This biosensor can be used as an initial diagnosis method to distinguish between healthy people and patients with periodontal diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Marta Kaminska, Malgorzata Benedyk-Machaczka, Karina Adamowicz, Ardita Aliko, Kamila Drzazga, Klaudia Slysz, Ewa Bielecka, Jan Potempa, Piotr Mydel
Summary: This study evaluated the antimicrobial effects of bestatin, a potential drug candidate for chronic periodontitis (CP). The results showed that bestatin exhibited bacteriostatic activity against periodontopathogens and controlled the formation and composition of the biofilm. Additionally, bestatin promoted the phagocytosis of periodontopathogens by immune cells and reduced inflammation. These findings suggest that bestatin is a promising choice for the treatment and prevention of periodontitis.
JOURNAL OF PERIODONTOLOGY
(2023)
Article
Microbiology
Mariusz Madej, Zuzanna Nowakowska, Miroslaw Ksiazek, Anna M. Lasica, Danuta Mizgalska, Magdalena Nowak, Anna Jacula, Monika Bzowska, Carsten Scavenius, Jan J. Enghild, Joseph Aduse-Opoku, Michael A. Curtis, F. Xavier Gomis-Ruth, Jan Potempa
Summary: The T9SS component PorZ interacts with sortase and specifically binds A-LPS, mediated by a phosphorylated branched mannan repeat in A-LPS polysaccharide. ALPS-bound PorZ interacts with sortase with significantly higher affinity, facilitating modification of cargo proteins by the cell surface attachment complex of the T9SS.
Review
Microbiology
Sara Sharaf, Karolin Hijazi
Summary: The development of periodontitis is a result of the interaction between bacterial virulence factors and immune responses, leading to inflammation and tissue degradation. This disease is associated with a shift in the oral microbiome, where harmless bacteria become pathogenic and evade immune responses. This review focuses on the genetic, transcriptional, and post-translational mechanisms that mediate this commensal-pathogen transition, with a particular emphasis on Porphyromonas gingivalis.
Article
Biotechnology & Applied Microbiology
Rui Li, Mengjie Hou, Liying Yu, Wen Luo, Jie Kong, Renmei Yu, Ruihan Liu, Qian Li, Lisi Tan, Chunling Pan, Hongyan Wang
Summary: This study aimed to investigate the effects of salivary histatin 5 (Hst5) on Porphyromonas gingivalis (P. gingivalis) biofilms in vitro and in vivo and the possible mechanisms. The results showed that 25μg/mL Hst5 effectively inhibited biofilm formation and increased concentrations of Hst5 increased the inhibitive effect. Hst5 could regulate membrane function and metabolic processes in P. gingivalis, and RpoD and FeoB proteins might play important roles in this process. Moreover, 100μg/mL Hst5 inhibited periodontal inflammation and alveolar bone loss in rat periodontitis through its antibacterial and anti-inflammatory effects.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Shuyu Guo, Jiawen Gu, Yue Jiang, Wenxiao Cui, Jialu Chen, Lu Li, Kai Zheng, Yan Xu
Summary: In this study, a new drug delivery strategy was developed to target Porphyromonas gingivalis (Pg)-accelerated atherosclerosis (AS). The biomimetic nanoparticles coated with Pg-treated macrophage membranes effectively eliminated Pg and resolved inflammation in Pg-infected regions, leading to a reduction in atheromatous plaque formation and alveolar bone loss.
MATERIALS & DESIGN
(2022)
Article
Biochemistry & Molecular Biology
Wendan He, Xianlong Xie, Chenxi Li, Huang Ding, Jishi Ye
Summary: This study investigated the role of SCH58261 in mice with chronic periodontitis-induced cognitive impairment. The results showed that SCH58261 could improve cognitive ability in mice, inhibit inflammatory responses and glutamate levels, and increase the expression of glutamate transporters. This suggests that SCH58261 may be used as a novel agent to treat cognitive impairment.
Article
Polymer Science
Wei-Fang Lee, Lu-Ying Wang, Ting-Yi Renn, Jen-Chang Yang, Lih-Sheng Fang, Yi-Huan Lee, Pei-Wen Peng
Summary: In this study, a polymeric substrate PEKK coated with 0.5% AgNPs was successfully synthesized and demonstrated to have antibacterial properties, making it suitable for implant applications.
Article
Chemistry, Organic
Fuying Zhu, Yamei Lin
Summary: In this study, a low-metal iron-supported catalyst (Fe20/NC-Mg) was reported for the efficient synthesis of quinoxaline compounds, using water as a solvent and without additional bases. The synergistic effect of FeNx site and Mg (OH)2 nanorods in the catalyst played a key role in achieving high yields (82-91%) in 16 examples. The gram-level synthesis and reusability of the catalysts after four cycles demonstrated its industrial application potential.
Article
Chemistry, Organic
Mikhail Kozlov, Alexey Tyurin, Andrey Dmitrenok, Vyacheslav Rusak, Aleksander Fedorov, Igor Zavarzin, Yulia Volkova
Summary: This study presents a novel and practical synthesis method for phosphorus-substituted 1,3,4-thiadiazolines using phosphorylthioformic acid hydrazides and ketones. The protocol demonstrates operational simplicity, availability of reagents, and tolerance towards different functional groups.
Article
Chemistry, Organic
Jisna Jose, Thomas Mathew
Summary: 2-Cyclopentenone and its derivatives are highly esteemed synthetic intermediates with exceptional utility in organic synthesis. They are favored structural motifs in numerous pharmaceutical drugs and natural products, highlighting their significance. The review discusses the various methods used to synthesize cyclopentenones from 2016 to 2023.