Review
Chemistry, Analytical
Katie M. Kohoutek, Peter de B. Harrington
Summary: Electrospray ionization ion mobility mass spectrometry (ESI-IMS-MS) is a rapidly progressing analytical technique that examines complex compounds in the gas phase. It separates isomers, provides structural information, and quantitatively identifies various compounds in biological samples. ESI-IMS-MS has wide applications in pharmaceutical, environmental, and manufacturing industries.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Chong Zhang, Kaitai Guo, Jiyao Wang, Tian Wang, Xiaohao Wang, Kai Ni
Summary: This study investigates the influence of electric field on the sensitivity of ion mobility spectrometry (IMS) and proposes an optimized design to enhance sensitivity. Experimental results demonstrate a significant improvement in the signal-to-noise ratio of DMMP dimer peak with the new design.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
C. Thoben, C-R Raddatz, M. Lippmann, Z. Salehimoghaddam, S. Zimmermann
Summary: This study investigated the performance of an electrospray ionization ion mobility spectrometer by varying the electric field strength in the desolvation region, introducing a new tristate ion shutter to enhance sensitivity for ions with higher molecular mass and lower ion mobility. The optimal ratio of electric field strengths in the drift and desolvation region was found to be affected by the electric field in the tristate ion shutter. The solvent flow rate also needs to be considered when setting the field strengths in the desolvation region to maintain proper electrospray function.
Article
Chemistry, Analytical
A. Bianco, I Neefjes, D. Alfaouri, H. Vehkamaki, T. Kurten, L. Ahonen, M. Passananti, J. Kangasluoma
Summary: Mass spectrometry is an effective method for identifying and quantifying environmentally relevant molecules, but it cannot distinguish between isomers. This study combines an experimental set-up using a differential mobility analyser with computational ion mobility calculations to successfully identify isomers. The experimental set-up allows for analysis in both the gas and liquid phase, making it a versatile tool for isomer separation.
Article
Chemistry, Multidisciplinary
Nina Khristenko, Frederic Rosu, Eric Largy, Jerome Haustant, Cedric Mesmin, Valerie Gabelica
Summary: Native ion mobility mass spectrometry has potential applications in the biophysical characterization of proteins, as the charge state distribution and collision cross section distribution depend on the solution conformation. This study investigates the charging and gas-phase conformation of multi-domain therapeutic proteins with globular domains tethered by disordered linkers. The results suggest a hybrid ionization mechanism, where globular domains are ejected one at a time from a parent droplet.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemical Research Methods
J. Michael Sutton, Noha M. El Zahar, Michael G. Bartlett
Summary: The study focuses on improving the mobile phase of electrospray oligonucleotides to reduce spectral complexity and increase sensitivity. By using different combinations of fluorinated alcohols and alkylamines, it is possible to selectively reduce the charge states of oligonucleotides. Additionally, anionic salts are used to stabilize complexes and provide unique structural information about the molecules.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Rebecca L. Cain, Ian K. Webb
Summary: Electrospray ionization mass spectrometry (ESI-MS) experiments were used to study the solution-like structures of intact proteins, specifically by controlling the protein unfolding process and analyzing folding intermediates. By mixing various pH solutions online with aqueous cytochrome C, the changes in charge states, arrival time distributions (ATDs), and electron capture dissociation (ECD) were measured to detect folding intermediates and unfolded protein structures.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yao Qian, Xiangyu Guo, Yunfang Wang, Zheng Ouyang, Xiaoxiao Ma
Summary: A mobility-modulated sequential dissociation (MMSD) strategy was developed for mass spectrometry imaging, enabling spatial lipidomics capable of multiplexed MS/MS imaging of 24 structurally distinct lipids. The method revealed a potential biomarker in a human hepatocellular carcinoma tissue.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Ziqi Fang, Yanfen Xu, Lian Duan, Yu Wei, Bo Hong, Ying Lv, Bo Yuan, Wen Liu, Guoxing Jing, Wenshan Li, Wenjie Liu, Jianna Yu
Summary: In this study, a method using ammonium acerate and secondary electrospray ionization ion mobility spectrometry (SESI-IMS) was evaluated for rapid determination of volatile biogenic amine contents in grass carp. The method showed minimum sample preparation and interference, and volatile biogenic amines displayed unique drift times without overlapping. The content of volatile biogenic amines correlated well with storage times and other freshness indicators.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Nattapong Chantipmanee, Peter C. Hauser
Summary: This article successfully demonstrated the electrospray ionization analysis of tobramycin using an open-source hardware ion mobility spectrometer. The instrument was assembled inexpensively in-house with very few purpose-made components. The quantitative determination of tobramycin only required 20 seconds and showed a linear calibration curve in the range of 50 to 200 µM. The method exhibited good reproducibility and a low limit of detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Max L. Feuerstein, Maykel Hernandez-Mesa, Andrea Kiehne, Bruno Le Bizec, Stephan Hann, Gaud Dervilly, Tim Causon
Summary: This study compared the collision cross section (CCS) derived from three different IM-MS technologies for the analysis of steroids and small molecules. The interlaboratory performance was found to be excellent, but differences in ion conformation sampled on different instrument types need further investigation.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Frederik Lermyte, Alina Theisen, Peter B. O'Connor
Summary: Biomolecular ions in mass spectrometry are characterized by their net charge, with methods existing to manipulate this quantity in the gas phase. The charge site of ions can significantly affect their gas-phase behavior, and research on this is particularly lacking for peptides and intact proteins. A study identified two distinct protonation site isomers of α-synuclein at the same charge state, showing differences in gas-phase fragmentation behavior and distinguishable by ion mobility spectrometry. Charge sites for both protomers were determined using ultraviolet photodissociation.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Environmental Sciences
Alina T. Roman-Hubers, Thomas J. McDonald, Erin S. Baker, Weihsueh A. Chiu, Ivan Rusyn
Summary: This study examined the potential of IMS-MS as a high-throughput method for the chemical characterization of crude oils, finding it to be either equal or better than GC-MS in classifying the origins of crude oils and greatly increasing sample analysis throughput. The study demonstrated the utility of IMS-MS for rapid fingerprinting of complex samples and showed its advantages over traditional GC-MS-based analyses in emergency decision-making situations.
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Karen E. Butler, Yoshihiko Takinami, Adam Rainczuk, Erin S. Baker, Blaine R. Roberts
Summary: Native mass spectrometry combined with IMS-MS technology can be used to study the tertiary and quaternary structure of proteins, validate other structural determination methods, and provide more comprehensive structural information.
FRONTIERS IN CHEMISTRY
(2021)
Review
Chemistry, Analytical
Daniel G. Delafield, Gaoyuan Lu, Cameron J. Kaminsky, Lingjun Li
Summary: Mass spectrometry-based biomolecular analyses have become popular in research settings. Gas phase ion separation techniques provide a fourth dimension of analysis, enabling rapid characterization and high throughput profiling. This review highlights the latest developments and applications of high-end ion-mobility enabled instruments and their advantages for biomolecular research.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ryoichi Nakagawa, Kai Saito, Hideyuki Kanematsu, Hidekazu Miura, Masatou Ishihara, Dana M. Barry, Takeshi Kogo, Akiko Ogawa, Nobumitsu Hirai, Takeshi Hagio, Ryoichi Ichino, Masahito Ban, Michiko Yoshitake, Stefan Zimmermann
Summary: Biofilms are the result of bacterial activity and a sensor that can detect biofilms is important. Graphene, which easily bonds with carbon compounds, could serve as a sensor for monitoring biofilm formation and growth. Previous research showed that CVD graphene film has higher sensitivity for biofilm formation. This new research focuses on preparing bilayer graphene with high mechanical strength to overcome the mechanical disadvantage of monolayer graphene when used as a biofilm sensor.
Article
Computer Science, Information Systems
Moritz Hitzemann, Kirsten J. Dehning, Adrian Gehl, Erk-Fietje Sterr, Stefan Zimmermann
Summary: Split-ring resonators (SRR) are simple electrical circuits that show a significant shift in resonance frequency even with the smallest changes in split capacitance. We present a new capillary split-ring resonator CaSRR, which is capable of accurately detecting very fast changes in split capacity. The proposed method is based on the analysis of the transmitted signal at a single frequency using demodulation.
Article
Computer Science, Information Systems
Moritz Hitzemann, Martin Lippmann, Jonas Trachte, Alexander Nitschke, Olaf Burckhardt, Stefan Zimmermann
Summary: This paper introduces a new concept of an isolated power supply for electronic components, with high insulation strength and power transmission, achieving high overall efficiency. Through the use of a coreless PCB transformer and investigation of different coil geometries, as well as comparison with a low-cost design, high efficiency and high insulation voltage are achieved.
Article
Chemistry, Analytical
Moritz Hitzemann, Christoph Schaefer, Ansgar T. Kirk, Alexander Nitschke, Martin Lippmann, Stefan Zimmermann
Summary: In this paper, a new and easy to assemble dielectric barrier discharge plasma ionization source is presented, which is based on printed circuit boards with two parallel isolated electrodes for generating plasma inside an inert fused silica capillary. The plasma source is coupled to an ion mobility spectrometer for demonstration. Different ionization pathways can be achieved by whether the analytes pass through the plasma or bypass it before entering the reaction region of the ion mobility spectrometer. The plasma source has been designed using electric field simulations and can reach detection limits in the mid pptv range.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Physical
Cameron N. Naylor, Christoph Schaefer, Ansgar T. Kirk, Stefan Zimmermann
Summary: Although the origin of the two peaks observed in ion mobility spectrometry (IMS) for aniline has been debated for 30 years, this study shows that they can be fully separated using high kinetic energy ion mobility spectrometry (HiKE-IMS) and are not in equilibrium. The speciation of the two aniline peaks is determined by the ionization source settings, and a third peak appears for certain fluoroanilines when the drift and reactant gas are changed to nitrogen, indicating increased separation. This provides valuable insights into the behavior of aniline and related compounds in IMS.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Analytical
Kirsten J. Dehning, Moritz Hitzemann, Alexander Gossmann, Stefan Zimmermann
Summary: Amino acids play a vital role in life as they are essential components of proteins and necessary for cell growth and maintenance. This study introduces a split-ring resonator as a sensitive and simple detector for amino acids. The split-ring resonator uses the electromagnetic properties of a liquid sample to determine the resonance frequency, and initial measurements show detection limits for tested amino acids ranging from 105 μM to 1564 μM. With an envelope detector, the split-ring resonator can be used in ion chromatography and achieve detection limits of 485 μM for aspartic acid and 956 μM for lysine at a flow rate of 0.5 mL/min.
Article
Chemistry, Analytical
Alexander Bohnhorst, Anne Zygmanowski, Yu Yin, Ansgar T. Kirk, Stefan Zimmermann
Summary: In this work, a tandem ion mobility spectrometer with a highly efficient ion manipulator is presented, allowing for the storage, manipulation, and analysis of ions under high electric field strengths and controlled ion-neutral reactions at ambient conditions. The instrument arrangement enables mobility selection, storage, and analysis of precursor ions, as well as mobility separation and detection of resulting product ions. The study demonstrates the capabilities of the instrument by characterizing eight different primary alcohols under various conditions, revealing fragmentation patterns that can be used for classification of unknown substances and gathering further information on product ion relations.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Christian Thoben, Christian-Robert Raddatz, Aykut Tataroglu, Tim Kobelt, Stefan Zimmermann
Summary: Every drift tube ion mobility spectrometer (IMS) has an optimum drift voltage to achieve maximum resolving power, which depends on the temporal and spatial width of the injected ion packet and the pressure within the IMS. By reducing the spatial width of the ion packet and slightly increasing the pressure, the performance of electrospray ionization (ESI)-IMS can be significantly improved, resulting in higher resolving power and better separation of different compounds.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Florian Schlottmann, Christoph Schaefer, Ansgar T. Kirk, Alexander Bohnhorst, Stefan Zimmermann
Summary: High Kinetic Energy Ion Mobility Spectrometers (HiKE-IMS) are operated at 20 mbar to achieve high reduced electric field strengths and improve linear range. Higher pressures promise increased sensitivity and smaller instrument size. In this study, theoretical requirements to avoid dielectric breakdown while maintaining high reduced electric field strengths at higher pressures were investigated. Experimental results led to the development of a HiKE-IMS operating at 60 mbar and achieving a limit of detection of 5 ppbV for n-hexane.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemical Research Methods
Christian Thoben, Nora T. Hartner, Moritz Hitzemann, Christian-Robert Raddatz, Manuel Eckermann, Detlev Belder, Stefan Zimmermann
Summary: The influence of solvents and buffer additives on the detected ion mobility peaks using ESI is investigated in this study. It is found that the ionization process during electrospray can be influenced by the choice of additives. By carefully selecting the solvent additives, the formation of different ion mobility peaks can be observed.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Anesthesiology
Simon Zumsande, Christian Thoben, Nils Dennhardt, Terence Krauss, Robert Suempelmann, Stefan Zimmermann, Henrik Rueffert, Sebastian Heiderich
Summary: This study evaluates the development of sevoflurane rebounds during trigger-free anesthesia. The results show that after guideline-compliant preparation, anesthetic machines may experience sevoflurane rebounds > 5 ppm during typical maneuvers used in clinical practice. The changes in internal gas flow during different ventilation modes and maneuvers could be possible explanations.
BMC ANESTHESIOLOGY
(2023)
Article
Biochemical Research Methods
Maximilian J. Kueddelsmann, Jannie J. Stadtler, Ansgar T. Kirk, Erik Bunert, Stefan Zimmermann
Summary: This paper presents a new electron capture detector (X-ECD) based on a compact X-ray tube for electron generation. The X-ECD has limits of detection in the pptv range and shows linearity over a wide concentration range. It is able to detect halogenated hydrocarbons and low volatile pesticides when combined with a conventional gas chromatograph.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Physical
Martin Zuehlke, Justin Koenig, Chris Pruefert, Stephan Sass, Toralf Beitz, Hans-Gerd Loehmannsroeben, Christian Thoben, Stefan Zimmermann, Raphael D. Urban, Bernd Abel
Summary: Precise control of chemical reaction conditions is important for environmental and resource management. In electrospray ionization, the dynamic changes in parameters significantly affect the reaction rate. The study focused on the tri-molecular Mannich reaction to investigate the impact of thermal, electronic, and fluidic manipulation on reaction rates compared to batch reactions.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
