Article
Chemistry, Analytical
Juan Chen, Mingyue Xie, Mingqing Shi, Kun Yuan, Yanan Wu, Hong-Min Meng, Lingbo Qu, Zhaohui Li
Summary: In this study, an accelerated DNA nanoprobe was developed for fast, in situ monitoring of miRNA in exosomes. The nanoprobe not only detects miRNA, but also distinguishes tumor exosomes from those derived from normal cells, and can be used to study exosome biological functions.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Jihen Elleuch, Faten Ben Amor, Mohamed Barkallah, Jihen Haj Salah, Kirsty F. Smith, Lotfi Aleya, Imen Fendri, Slim Abdelkafi
Summary: A quantitative-PCR assay based on the ITS molecular marker was developed for detecting and quantifying K. selliformis, with high specificity and a low detection limit. This assay shows potential for field application in monitoring K. selliformis blooms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Thanh-Luu Pham, Thi Hoang Yen Tran, Kazuya Shimizu, Qintong Li, Motoo Utsumi
Summary: Toxic cyanobacterial blooms are a global concern, and this study focused on the dynamics of toxic cyanobacteria and microcystin concentrations in the Tri An Reservoir in Vietnam. Results showed that Microcystis was the dominant group and primary microcystin producer, with environmental factors such as nutrient loading and suspended solids influencing blooms and toxic genotype prevalence.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Food Science & Technology
Epaminondas D. Christou, Ioanna Varkitzi, Isabel Maneiro, Soultana Zervoudaki, Kalliopi Pagou
Summary: Researchers conducted a series of experiments to study the effects of toxic dinoflagellate on the feeding, reproduction, and toxin excretion of copepods. They found that the toxicity of the dinoflagellate affected the reproduction, toxin excretion, and feeding behavior of the copepods, indicating the significant impact of toxins on copepods.
Article
Materials Science, Multidisciplinary
Audrey Wang, Xindan Zhang, Haoran Shi, Youngkwan Lee, Zhenqi Jiang, Soochan Kim, Jieling Qin
Summary: Mycoplasma contamination in cell/tissue cultures can significantly affect cell characteristics and drug reactions without obvious changes in morphology or growth rates. A newly developed electrochemical screening system offers rapid, accurate, and convenient detection of Mycoplasma at low concentrations.
MATERIALS TODAY ADVANCES
(2023)
Review
Chemistry, Multidisciplinary
Barathan Balaji-Prasath, Ying Wang, Yu Ping Su, David P. Hamilton, Hong Lin, Luwei Zheng, Yong Zhang
Summary: This article reviews physical, chemical, and biological control methods applicable to red tide species in marine and estuarine water bodies, and discusses the mechanisms of algal bloom outbreaks and their applications.
ENVIRONMENTAL CHEMISTRY LETTERS
(2022)
Article
Biotechnology & Applied Microbiology
Chang-ye Hui, Yan Guo, Chao-xian Gao, Han Li, Yi-ran Lin, Jian-pei Yun, Yu-ting Chen, Juan Yi
Summary: A visual whole-cell biosensor was developed for detecting heavy metal pollutants, with a strong response to Cd(II) and the ability to detect low concentrations at different growth stages. The biosensor showed advantages of high-throughput detection and approximate quantification.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Marine & Freshwater Biology
Zabdiel Roldan Ayala, Savannah Judge, Silvia Angles, Dianne I. Greenfield
Summary: Light microscopy, FlowCam, and sandwich hybridization assay (SHA) were compared for monitoring harmful algal bloom (HAB) forming phytoplankton. The results showed that these techniques returned comparable cell concentrations, but SHA produced non-detect signals <2 cells mL-1 in field samples and the FlowCam slightly underestimated cell concentrations when abundances were high. The findings are important for HAB researchers, managers, and public health officials in reconciling cell abundance datasets and enhancing HAB monitoring and prediction.
Article
Chemistry, Analytical
Xiu Liu, Shengjun Bu, Hongguo Wei, Ze Wang, Songling Yu, Zhongyi Li, Zhuo Hao, Xiuxia He, Jiayu Wan
Summary: A simple, sensitive, and enzyme-free method for visual point-of-care detection of 16S rRNA of Escherichia coli O157:H7 has been developed based on an isothermal strand displacement-hybrid chain reaction (ISD-HCR) and lateral flow strip (LFS). This approach allows for detection of E. coli O157:H7 at concentrations as low as 10(2) CFU mL(-1) without the need for instrumentation, providing a valuable tool for point-of-care detection of pathogenic bacteria in resource-limited countries.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Jinshan Yuan, Guoming Xie, Junjie Li, Yaxing Xie, Yuan Yao, Jianhong Zhang, Yulei Hou, Hui Chen
Summary: A fluorescent sensing platform was developed for the simple and sensitive monitoring of vancomycin, using an allosteric probe that undergoes a conformational change in the presence of vancomycin. This platform has the potential to support therapeutic drug monitoring of vancomycin, promoting the rational use of antibiotics in clinics.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biotechnology & Applied Microbiology
Barathan Balaji-Prasath, Ying Wang, Yuping Su, Minghua Chen, Yi Zheng
Summary: The fermentation processes of algicidal bacteria offer a promising and eco-friendly approach for controlling harmful algae blooms (HABs). This study analyzed the microbial fermentation of algicidal bacterium Ba3 and its potential ability to produce algicidal compounds. The results show that the fermentation products have the potential to control dinoflagellate blooms, providing an encouraging basis for large-scale fermentation production and the control of red tide outbreaks.
FERMENTATION-BASEL
(2022)
Review
Engineering, Chemical
Reyna Berenice Gonzalez-Gonzalez, Elda A. Flores-Contreras, Everardo Gonzalez-Gonzalez, Nora E. Torres Castillo, Roberto Parra-Saldivar, Hafiz M. N. Iqbal
Summary: Water and soil contamination is a global environmental concern that affects food and water security. Biosensors have shown effectiveness in accurate, on-site, and real-time detection of various pollutants. Different bioreceptors such as cells, antibodies, aptamers, enzymes, and nanozymes have been explored, exhibiting excellent performance. This review discusses the recent advances in each type of biosensor and compares different strategies and challenges for detecting pollutants.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Chemistry, Analytical
Dedi Futra, Ling Ling Tan, Su Yin Lee, Benchaporn Lertanantawong, Lee Yook Heng
Summary: An ultrasensitive electrochemical DNA biosensor was developed for rapid detection of Vibrio cholerae DNA in environmental samples. The biosensor utilized silica nanospheres functionalized with 3-aminopropyltriethoxysilane and gold nanoparticles for effective immobilization of the capture probe and acceleration of electron transfer. The targeted DNA sequence of V. cholerae was detected using a sandwich hybridization strategy with a pair of DNA probes and evaluated by differential pulse voltammetry.
Review
Chemistry, Multidisciplinary
Islam M. El-Sewify, Ahmed Radwan, Nehal H. Elghazawy, Wolfgang Fritzsche, Hassan M. E. Azzazy
Summary: Alzheimer's disease is the most common type of dementia, and heavy metals in water may be connected to its onset. Optical chemosensors offer advantages in detecting toxic metals in water, contributing to the fight against AD.
Article
Biodiversity Conservation
Juan Li, Peter Seeber, Jan Axtner, Rachel Crouthers, Milou Groenenberg, Arnulf Koehncke, Alexandre Courtiol, Pin Chanratana, Alex D. Greenwood
Summary: Observation-based methods in wildlife monitoring are limited to well-characterized species, while environmental DNA (eDNA) can complement them by detecting multiple taxa with less labor-intensive sample collection. Hybridization capture and metabarcoding are two eDNA methods with different strengths, and combining them can improve sensitivity and species detection.
BIOLOGICAL CONSERVATION
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).