期刊
TALANTA
卷 152, 期 -, 页码 475-481出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2016.02.043
关键词
Vortex-assisted liquid-liquid microextraction; Derivatization; Amine; Naphthalene-2,3-dicarboxaldehyde; HPLC; Fluorometric determination
资金
- Ministry of Science and Technology, Taiwan, Republic of China [NSC 100-2113-M-007-004, NSC 101-2113-M-007-011]
A highly sensitive liquid chromatographic method was developed for the fluorometric determination of trace amounts of linear aliphatic primary amines. Prior to extraction, amines were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) in the presence of cyanide ion (CN) and extracted by vortex assisted liquid-liquid microextraction (VALLME). The optimum conditions were as follows: derivatization reaction time for 5 min in 2.0 mL aqueous donor samples with 50 mu M NDA/CN, and 10 mM borate buffer at pH 9; vortex extraction time for 20 s in the VALLME step with 50 mu L of isooctane as the extractant phase; centrifugation for 1 min at 6000 rpm. Under the optimum conditions, the limits of detection (LOD) were between 0.01 and 0.04 nmol L-1. The calibration curves showed good linearity in the range of 0.1-20 nmol L-1. In comparison with previous work using o-phthalaldehyde/2-mercaptoethanol derivatization, the method has much more stable fluorescent derivatives, higher fluorescence intensities, and greater extraction efficiencies. The sensitivity enhancement factors (SEF) were between 2 and 70, which is in good agreement with the theoretical values calculated from partition coefficients in VALLME system. (C) 2016 Elsevier B.V. All rights reserved.
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