Article
Chemistry, Analytical
Renata Konasova, Maria Butnariu, Veronika Solinova, Vaclav Kasicka, Dusan Koval
Summary: Charged polymer coatings on the inner fused silica capillary wall enable tunable electroosmotic flow velocity for optimizing peak resolution and separation efficiency in capillary electrophoresis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Nuzhet Nihaar Nasir Ahamed, Carlos A. Mendiola-Escobedo, Olivia D. Ernst, Victor H. Perez-Gonzalez, Blanca H. Lapizco-Encinas
Summary: This study demonstrates that electrokinetic phenomena can be used to develop rapid and reliable methods for microparticle and cell assessments in a cost-effective and label-free manner. By using direct current (DC)-biased low-frequency alternating current (AC) voltages in an insulator-based electrokinetic (iEK) system, microparticles with a small difference in zeta potentials can be effectively separated.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
M. Hannah Choi, Liu Hong, Leonardo P. Chamorro, Boyd Edwards, Aaron T. Timperman
Summary: The ability to measure the charge and size of single particles is crucial for understanding particle adhesion and interaction with the environment. The new technique called TrACE combines particle tracking velocimetry (PTV) and AC electrophoresis to accurately measure the charge and size of particles. This technique shows promise in studying the physical properties of cells and has potential applications in various fields.
Review
Chemistry, Analytical
Piotr Kowalski, Ilona Oledzka, Alina Plenis, Anna Roszkowska, Tomasz Baczek
Summary: This article discusses the applications of ionic liquids (ILs) in capillary electrophoresis (CE). ILs are used to modify the electroosmotic flow in CE by influencing the silanol groups on the capillary surface. The interactions between ILs and analytes can change the separation mechanism and efficiency of CE, making ILs important additives in CE.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Andaravaas Patabadige Jude P. Vaas, Joselito P. Quirino
Summary: A stacking technique using CTAB and SDS was proposed to enhance the detection sensitivity of capillary zone electrophoresis (CZE) with UV detection. The method involves enriching and releasing analytes from the inner walls of a fused silica capillary by forming catanionic micelles, leading to improved detection sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Natalia Kaczmarczyk, Julita Cizewska, Natalia Treder, Natalia Miekus, Alina Plenis, Piotr Kowalski, Anna Roszkowska, Tomasz Baczek, Ilona Oledzka
Summary: This study evaluates the impact of using ionic liquids (ILs) on the separation efficiency of biogenic amines (BAs) in micellar electrokinetic chromatography (MEKC). It found that ILs with chloride and tetrafluoroborate ions were the most effective, while ILs with trifluoromethanesulfonate and bis(trifluoromethylsulfonyl)imide ions disrupted the separation process. Overall, this study provides a critical assessment of the different types and concentrations of ILs in MEKC analysis of selected BAs.
Article
Chemistry, Analytical
Niu Feng, Xuewen Peng, Zhipan Wang, Xiaoping Yu, Xuping Shentu, Yiping Chen
Summary: Pursuing convenient operations and precise testing has become an urgent requirement in clinical diagnosis, treatment, and prognosis. This study constructed a label-free electroosmotic flow-driven microchannel (LF-EMB) biosensor based on the dual properties of highly selective antibody-antigen recognition and potential signaling of biomolecules. The LF-EMB demonstrated outstanding sensitivity and detection speed, providing a convenient option for the quantitative detection of inflammatory markers and antibiotics.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Andaravaas Patabadige Jude P. Vaas, Raymond B. Yu, Joselito P. Quirino
Summary: Cyclodextrins are proposed as a pseudophase in pseudophase-to-pseudophase microextraction in capillary zone electrophoresis. This method, known as CD to admicelle ME, involves the injection of a dilute analyte solution into the capillary, forming admicelles at the interface. CD solution is then injected to release the analytes from the capillary surface, resulting in concentration of the analytes. The method shows enhanced sensitivity and has potential for analysis of urine samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Pingjing Li, Lulu Wang, Rui Guo, Huatao Feng, Ya Ji, Si Ying Lim, Bao Hui Ng, Anna Karen Carrasco Laserna, Shaheer Khan, Shiaw-Min Chen, Sam Fong Yau Li
Summary: Recombinant protein biopharmaceuticals are important in current drug development, and the glycosylation profile of these proteins plays a crucial role in their quality. This study developed a CE-LIF method for accurate glycan identification by cross-identification using two capillary coatings and three labeling dyes. The use of homemade PVA and commercial coatings showed similar separation profiles, and the combination of fluorescent dyes provided better accuracy.
Article
Biochemical Research Methods
Eva Martinkova, Tomas Krizek, Anna Kubickova, Pavel Coufal
Summary: UV-absorbing neutral substances are commonly used as markers of mean electroosmotic flow in capillary electrophoresis for their zero electrophoretic mobility in an electric field. However, some of these markers can interact with background electrolyte components and migrate at a different velocity than the electroosmotic flow. We tested 11 markers primarily varying in their degree of methylation and type of central atom in combination with five background electrolyte cations differing in their ionic radii and surface charge density, measuring the relative electrophoretic mobility using thiourea as a reference marker. Our results from this set of experiments showed some general trends in the mobilization of the markers based on the effects of marker structure and type of background electrolyte cation on the relative electrophoretic mobility. As an example, the effects of an inadequate choice of marker on analyte identification were illustrated in the electrophoretic separation of glucosinolates. Therefore, our findings may help electrophoretists appropriately select electroosmotic flow markers for various electrophoretic systems.
Article
Chemistry, Physical
Lijian Zhan, Yin Zhang, Wei Si, Jingjie Sha, Yunfei Chen
Summary: This study demonstrates a solid-state nanopore platform for label-free detection and separation of short single-stranded DNA fragments based on length-dependent translocation behaviors. The controllable selectivity of ssDNA can be achieved by tuning nanopore size, with a maximum selectivity limitation of 30nt.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Review
Biotechnology & Applied Microbiology
Amadeo Sena-Torralba, Yulieth D. Banguera-Ordonez, Laia Mira-Pascual, Angel Maquieira, Sergi Morais
Summary: To decentralize health care, the development of point-of-care (PoC) assays has gained attention. The lateral flow immunoassay (LFIA) is seen as a promising bioanalytical method due to its low cost and single-step detection process. However, its limited sensitivity and inability to detect disease biomarkers at clinically relevant levels have limited its application. This review explores the potential of merging different electrokinetic phenomena into paper-based assays to enhance their analytical performance.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Mostafa A. Atia, Petr Smejkal, Vipul Gupta, Paul R. Haddad, Michael C. Breadmore
Summary: This study demonstrates the superior performance of chemical vapour deposition (CVD) coating for suppressing the electroosmotic flow (EOF) compared to liquid phase deposition (LPD) in capillary electrophoresis (CE). By adjusting the modification method and surface chemistry, the capillary can be easily and effectively modified, and the stability of the coating meets the long-term monitoring requirements for water chemistry.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Veronika Solinova, Petr Tuma, Maria Butnariu, Vaclav Kasicka, Dusan Koval
Summary: We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. The method involves surface modification of the separation fused silica capillary using a covalently attached copolymer to control the charge density and achieve adjustable EOF. The properties of EOF in the treated capillaries are observed and the importance of adjustable EOF in separations is demonstrated.
Article
Biochemical Research Methods
Raymond B. Yu, Joselito P. Quirino
Summary: The application of ionic liquids as additives in EKC plays an important role in chiral separations, as evidenced by numerous research articles. Studies show that the use of ionic liquids can improve separation efficiency, with further research needed to validate their mechanisms of action.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Chemistry, Analytical
Julien Camperi, Valerie Pichon, Nathalie Delaunay
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Chemistry, Analytical
Stan Perchepied, Nicolas Eskenazi, Chiara Giangrande, Julien Camperi, Thierry Fournier, Joelle Vinh, Nathalie Delaunay, Valerie Pichon
Article
Biochemical Research Methods
Julien Camperi, Audrey Combes, Thierry Fournier, Valerie Pichon, Nathalie Delaunay
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Amira Al Matari, Audrey Combes, Julien Camperi, Thierry Fournier, Valerie Pichon, Nathalie Delaunay
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Analytical
Thomas Bouvarel, Nathalie Delaunay, Valerie Pichon
Summary: This review discusses the latest developments in miniaturization of molecularly imprinted polymers to meet the societal demand for reliable, fast, affordable, and solvent/sample saving analyses. Different polymerization routes for producing miniaturized molecularly imprinted polymers in capillaries or chip channels are investigated, along with their potential applications in various separation techniques.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Biochemical Research Methods
Amira Al Matari, Anastasia Goumenou, Audrey Combes, Thierry Fournier, Valerie Pichon, Nathalie Delaunay
Summary: The hCG protein, consisting of alpha and beta subunits with various glycoforms, was previously analyzed for glycoforms at the intact level. A modified method now allows for improved separation and detection of hCG alpha and beta glycoforms, with a higher number of glycoforms detected. The new method shows potential for rapid quality control of glycoprotein-based pharmaceutical preparations.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Chemistry, Analytical
Maria Vergara-Barberan, Maria Jesus Lerma-Garcia, Ancuta Moga, Enrique Javier Carrasco-Correa, Hector Martinez-Perez-Cejuela, Miriam Beneito-Cambra, Ernesto Francisco Simo-Alfonso, Jose Manuel Herrero-Martinez
Summary: Sample preparation is essential in food analysis to achieve adequate extraction and preconcentration of target analytes. Aptamer-based sorbents have shown great potential in isolating and determining various compounds in different food matrices.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Thomas Bouvarel, Christophe Chendo, Nathalie Delaunay, Valerie Pichon
Summary: A simple, selective, and sensitive method for determining benzoylecgonine in complex biological samples was developed, utilizing a miniaturized solid phase extraction step with a monolithic molecularly imprinted polymer directly coupled to UV detection. The method achieved high selectivity and reproducibility, with limits of detection and quantification meeting regulatory standards for BZE detection in urine without the need for additional chromatographic separation. The photopolymerization technique allowed for the production of monoliths of varying lengths to adapt to different applications.
Review
Biochemistry & Molecular Biology
Anastasia Goumenou, Nathalie Delaunay, Valerie Pichon
Summary: Glycosylation is a significant post-translational modification of proteins that can affect their basic properties, but a full characterization of glycosylation remains challenging due to sample complexity. Lectins, a group of proteins with affinity for specific sugar moieties, are commonly used for selective enrichment in affinity chromatography and solid phase extraction methods.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Biochemical Research Methods
Hector Martinez-Perez-Cejuela, Enrique Javier Carrasco-Correa, Ancuta Moga, Maria Vergara-Barberan, Miriam Beneito-Cambra, Maria Jesus Lerma-Garcia, Ernesto Francisco Simo-Alfonso, Jose Manuel Herrero-Martinez
Summary: In recent years, there have been significant efforts in miniaturization, with the emergence of formats such as chips, 3D-printed objects, and paper-based devices. These systems have been utilized in biological and chemical processes, leveraging their advantages of waste reduction, low cost, and portability. However, there is a need to further develop more user-friendly devices with improved performance to address current analytical challenges. This review aims to address this research gap by highlighting the recent contributions of reticular porous materials, such as metal-organic frameworks (MOFs) and covalent-organic frameworks (COFs), in these miniaturized and emerging formats.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Environmental Sciences
Marine Boudias, Alkiviadis Gourgiotis, Gilles Montavon, Charlotte Cazala, Valerie Pichon, Nathalie Delaunay
Summary: The article discusses the performances of different analytical approaches, followed by an overview of applications.
JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
(2022)
Article
Chemistry, Analytical
Pengchao Cao, Valerie Pichon, Catherine Dreanno, Kada Boukerma, Nathalie Delaunay
Summary: Several ion-imprinted polymers (IIPs) were synthesized for selective extraction of Cu(II) ions. The best IIP exhibited high specificity and good selectivity, with an adsorption capacity of 63 μg g(-1) and an enrichment factor of 24. The method was successfully applied to real samples and Brunauer-Emmett-Teller analysis showed the impact of post-treatment on the porous structure.
Article
Biochemical Research Methods
Amira Al Matari, Christophe Chendo, Thomas Bouvarel, Mael Arveiler, Michael Tatoulian, Olivier Venier, Nathalie Delaunay, Valerie Pichon
Summary: Monitoring synthesis reactions in real time is important for detecting intermediates, understanding reaction mechanisms, and identifying impurities. Spectroscopic methods may not be effective for complex mixtures, while mass spectrometry (MS) offers accuracy and sensitivity for detecting, quantifying, identifying, and characterizing reagents and species. This study developed a universal setup using two valves to hyphenate a microreactor with MS, which showed good repeatability and a linear response for analyzing molecules of different nature and hydrophilicity, even in non-MS-compatible solvents. The setup allowed for monitoring compounds synthesized at different concentrations and was successfully used in studying an amination reaction with high repeatability.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Marine Boudias, Sofiane Korchi, Alkiviadis Gourgiotis, Audrey Combes, Charlotte Cazala, Valerie Pichon, Nathalie Delaunay
Summary: In this study, a selective and specific sorbent for radium extraction was developed using ion-imprinted polymer technology. The researchers identified the best synthesis conditions by testing various parameters and comparing the properties of different polymers. The resulting ion-imprinted polymer showed both selectivity and specificity in retaining radium ions while minimizing interference from other ions.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Chemistry, Analytical
Miriam Beneito-Cambra, David Moreno-Gonzalez, Juan F. Garcia-Reyes, Marcos Bouza, Bienvenida Gilbert-Lopez, Antonio Molina-Diaz
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).