期刊
STEM CELL RESEARCH
卷 17, 期 3, 页码 498-503出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scr.2016.09.025
关键词
Let-7; LIN28; Pluripotency; Human embryonic stem cell
资金
- Turku Doctoral Programme of Biomedical Sciences
- Finnish Cancer Organization
- Hospital District of Southwest Finland
- Finnish Cultural Foundation
- Emil Aaltonen Foundation
- Ida Montin Foundation
- Waldemar von Frenckell Foundation
- Finnish-Norwegian medical Foundation
- Sigrid Juselius Foundation
- Paulo Foundation
- Academy of Finland Centre of Excellence in Molecular Systems Immunology and Physiology Research
- Academy of Finland [123322, 116713, 250114]
- Academy of Finland (AKA) [250114, 123322, 116713, 250114, 116713, 123322] Funding Source: Academy of Finland (AKA)
MicroRNAs (miRNA) are central regulators of diverse biological processes and are important in the regulation of stem cell self-renewal. One of the widely studied miRNA-protein regulators is the Lin28-Let-7 pair. In this study, we demonstrate that contrary to the well-established models of mouse ES cells (mESC) and transformed human cancer cells, the pluripotent state of human ES cells (hESC) involves expression of mature Let-7 family miRNAs with concurrent expression of all LIN28 proteins. We show that mature Let-7 miRNAs are regulated during hESC differentiation and have opposite expression profile with LIN28B. Moreover, mature Let-7 miRNAs fine tune the expression levels of LIN28B protein in pluripotent hESCs, whereas silencing of LIN28 proteins have no effect on mature Let-7 levels. These results bring novel information to the highly complex network of human pluripotency and suggest that maintenance of hESC pluripotency differs greatly from the mESCs in regard to LIN28-Let-7 regulation. (C) 2016 The Authors. Published by Elsevier B.V.
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