期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 161, 期 -, 页码 64-69出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2016.02.028
关键词
Flucloxacillin; Amoxicillin; Ratio subtraction; Absorbance subtraction; Amplitude modulation
类别
Three different spectrophotometric methods were applied for the quantitative analysis of flucloxacillin and amoxicillin in their binary mixture, namely, ratio subtraction, absorbance subtraction and amplitude modulation. A comparative study was done listing the advantages and the disadvantages of each method. All the methods were validated according to the ICH guidelines and the obtained accuracy, precision and repeatability were found to be within the acceptable limits. The selectivity of the proposed methods was tested using laboratory prepared mixtures and assessed by applying the standard addition technique. So, they can be used for the routine analysis of flucloxacillin and amoxicillin in their binary mixtures. (C) 2016 Elsevier B.V. All rights reserved.
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